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Full-Text Articles in Molecular Biology

Investigating The Essential Roles Of Dprl-1 In Drosophila Melanogaster, Alex Lee Jan 2017

Investigating The Essential Roles Of Dprl-1 In Drosophila Melanogaster, Alex Lee

Summer Research

Phosphatase of Regenerating Liver (PRL) proteins regulate a number of important cellular processes, including cell growth and division. Humans have three PRL proteins: PRL-1, PRL-2, and PRL-3. An accumulation of evidence has shown that elevated levels of PRLs are strongly correlated with uncontrollable growth and metastasis of tumors. However, contradictory findings have arisen indicating that PRLs instead function to halt cell division thereby preventing uncontrollable tumor growth. In light of these results, the underlying mechanisms regarding how PRLs function within cellular processes remains unclear. To investigate the functions of PRLs, we will create transgenic fruit flies (Drosophila melanogaster) with knockout ...


24 Hour Incubation Of Ovary Tissue In 17Α20Β-Dihyroxy-4-Pregnon-3-One Causes Bcl-2 And Pi3k-Iii Expression To Vary In Danio Rerio, Beth Onaga Jan 2014

24 Hour Incubation Of Ovary Tissue In 17Α20Β-Dihyroxy-4-Pregnon-3-One Causes Bcl-2 And Pi3k-Iii Expression To Vary In Danio Rerio, Beth Onaga

Summer Research

The present study investigated the effects of increasing concentrations of progesterone on cell death by monitoring the expression of mRNAs specific for genes in the apoptosis and autophagy cell death pathway in Danio rerio ovary tissue using quantitative (q)PCR. Progesterone is used to regulate oocyte maturation in vivo; however, synthetic progesterone in oral contraceptives is found in the environment. Primers for Bcl-2 and Pi3K-III were used to investigate gene activity via mRNA produced. The results of the present study revealed that gene activity was variable as revealed by qPCR analysis. After 24 hour exposure to progesterone Bcl-2 and Pi3K-III ...