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Full-Text Articles in Molecular Biology

Association Of Modified Cytosines And The Methylated Dna-Binding Protein Mecp2 With Distinctive Structural Domains Of Lampbrush Chromatin, Garry T. Morgan, Peter L. Jones, Michel Bellini Dec 2012

Association Of Modified Cytosines And The Methylated Dna-Binding Protein Mecp2 With Distinctive Structural Domains Of Lampbrush Chromatin, Garry T. Morgan, Peter L. Jones, Michel Bellini

Peter Jones Lab Publications

We have investigated the association of DNA methylation and proteins interpreting methylation state with the distinctive closed and open chromatin structural domains that are directly observable in the lampbrush chromosomes (LBCs) of amphibian oocytes. To establish the distribution in LBCs of MeCP2, one of the key proteins binding 5-methylcytosine-modified DNA (5mC), we expressed HA-tagged MeCP2 constructs in Xenopus laevis oocytes. Full-length MeCP2 was predominantly targeted to the closed, transcriptionally inactive chromomere domains in a pattern proportional to chromomeric DNA density and consistent with a global role in determining chromatin state. A minor fraction of HA-MeCP2 was also found to associate ...


C. Elegans Pat-9 Is A Nuclear Zinc Finger Protein Critical For The Assembly Of Muscle Attachments, Qian Liu, Takako I. Jones, Rebecca A. Bachmann, Mitchell Meghpara, Lauren Rogowski, Benjamin D. Williams, Peter L. Jones May 2012

C. Elegans Pat-9 Is A Nuclear Zinc Finger Protein Critical For The Assembly Of Muscle Attachments, Qian Liu, Takako I. Jones, Rebecca A. Bachmann, Mitchell Meghpara, Lauren Rogowski, Benjamin D. Williams, Peter L. Jones

Peter Jones Lab Publications

BACKGROUND: Caenorhabditis elegans sarcomeres have been studied extensively utilizing both forward and reverse genetic techniques to provide insight into muscle development and the mechanisms behind muscle contraction. A previous genetic screen investigating early muscle development produced 13 independent mutant genes exhibiting a Pat (paralyzed and arrested elongation at the two-fold length of embryonic development) muscle phenotype. This study reports the identification and characterization of one of those genes, pat-9.

RESULTS: Positional cloning, reverse genetics, and plasmid rescue experiments were used to identify the predicted C. elegans gene T27B1.2 (recently named ztf-19) as the pat-9 gene. Analysis of pat-9 showed ...