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Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Developmental Biology

University of Massachusetts Medical School

Program in Molecular Medicine Publications and Presentations

2016

Articles 1 - 2 of 2

Full-Text Articles in Molecular Biology

Staufen Negatively Modulates Microrna Activity In Caenorhabditis Elegans, Zhiji Ren, Isana Veksler-Lublinsky, David Morrissey, Victor R. Ambros Feb 2016

Staufen Negatively Modulates Microrna Activity In Caenorhabditis Elegans, Zhiji Ren, Isana Veksler-Lublinsky, David Morrissey, Victor R. Ambros

Program in Molecular Medicine Publications and Presentations

The double-stranded RNA-binding protein Staufen has been implicated in various post-transcriptional gene regulatory processes. Here we demonstrate that the Caenorhabditis elegans homolog of Staufen, STAU-1, functionally interacts with microRNAs. Loss-of-function mutations of stau-1 significantly suppress phenotypes of let-7 family microRNA mutants, a hypomorphic allele of dicer and a lsy-6 microRNA partial loss-of-function mutant. Furthermore, STAU-1 modulates the activity of lin-14, a target of lin-4 and let-7 family microRNAs, and this modulation is abolished when the 3' untranslated region of lin-14 is removed. Deep sequencing of small RNA cDNA libraries reveals no dramatic change in the levels of microRNAs, or other ...


Tenomodulin Promotes Human Adipocyte Differentiation And Beneficial Visceral Adipose Tissue Expansion, Ozlem Senol-Cosar, Rachel J. Roth Flach, Marina Distefano, Anil K. Chawla, Sarah M. Nicoloro, Juerg R. Straubhaar, Olga T. Hardy, Hye Lim Noh, Jason K. Kim, Martin Wabitsch, Philipp E. Scherer, Michael P. Czech Feb 2016

Tenomodulin Promotes Human Adipocyte Differentiation And Beneficial Visceral Adipose Tissue Expansion, Ozlem Senol-Cosar, Rachel J. Roth Flach, Marina Distefano, Anil K. Chawla, Sarah M. Nicoloro, Juerg R. Straubhaar, Olga T. Hardy, Hye Lim Noh, Jason K. Kim, Martin Wabitsch, Philipp E. Scherer, Michael P. Czech

Program in Molecular Medicine Publications and Presentations

Proper regulation of energy storage in adipose tissue is crucial for maintaining insulin sensitivity and molecules contributing to this process have not been fully revealed. Here we show that type II transmembrane protein tenomodulin (TNMD) is upregulated in adipose tissue of insulin-resistant versus insulin-sensitive individuals, who were matched for body mass index (BMI). TNMD expression increases in human preadipocytes during differentiation, whereas silencing TNMD blocks adipogenesis. Upon high-fat diet feeding, transgenic mice overexpressing Tnmd develop increased epididymal white adipose tissue (eWAT) mass, and preadipocytes derived from Tnmd transgenic mice display greater proliferation, consistent with elevated adipogenesis. In Tnmd transgenic mice ...