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Developmental Biology

University of Massachusetts Medical School

Peter Jones Lab Publications

Pat

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Full-Text Articles in Molecular Biology

C. Elegans Pat-9 Is A Nuclear Zinc Finger Protein Critical For The Assembly Of Muscle Attachments, Qian Liu, Takako I. Jones, Rebecca A. Bachmann, Mitchell Meghpara, Lauren Rogowski, Benjamin D. Williams, Peter L. Jones May 2012

C. Elegans Pat-9 Is A Nuclear Zinc Finger Protein Critical For The Assembly Of Muscle Attachments, Qian Liu, Takako I. Jones, Rebecca A. Bachmann, Mitchell Meghpara, Lauren Rogowski, Benjamin D. Williams, Peter L. Jones

Peter Jones Lab Publications

BACKGROUND: Caenorhabditis elegans sarcomeres have been studied extensively utilizing both forward and reverse genetic techniques to provide insight into muscle development and the mechanisms behind muscle contraction. A previous genetic screen investigating early muscle development produced 13 independent mutant genes exhibiting a Pat (paralyzed and arrested elongation at the two-fold length of embryonic development) muscle phenotype. This study reports the identification and characterization of one of those genes, pat-9.

RESULTS: Positional cloning, reverse genetics, and plasmid rescue experiments were used to identify the predicted C. elegans gene T27B1.2 (recently named ztf-19) as the pat-9 gene. Analysis of pat-9 showed ...