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Developmental Biology

University of Massachusetts Medical School

Peter Jones Lab Publications

Lampbrush chromosomes

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Full-Text Articles in Molecular Biology

Association Of Modified Cytosines And The Methylated Dna-Binding Protein Mecp2 With Distinctive Structural Domains Of Lampbrush Chromatin, Garry T. Morgan, Peter L. Jones, Michel Bellini Dec 2012

Association Of Modified Cytosines And The Methylated Dna-Binding Protein Mecp2 With Distinctive Structural Domains Of Lampbrush Chromatin, Garry T. Morgan, Peter L. Jones, Michel Bellini

Peter Jones Lab Publications

We have investigated the association of DNA methylation and proteins interpreting methylation state with the distinctive closed and open chromatin structural domains that are directly observable in the lampbrush chromosomes (LBCs) of amphibian oocytes. To establish the distribution in LBCs of MeCP2, one of the key proteins binding 5-methylcytosine-modified DNA (5mC), we expressed HA-tagged MeCP2 constructs in Xenopus laevis oocytes. Full-length MeCP2 was predominantly targeted to the closed, transcriptionally inactive chromomere domains in a pattern proportional to chromomeric DNA density and consistent with a global role in determining chromatin state. A minor fraction of HA-MeCP2 was also found to associate ...