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Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Developmental Biology

University of Massachusetts Medical School

Peter Jones Lab Publications

Animals; Cell Differentiation; Chromatin; DNA; DNA Methylation; Embryo, Nonmammalian; Embryonic Development; Embryonic Stem Cells; Gene Expression Regulation, Developmental; Histones; Humans; Promoter Regions, Genetic; *Transcription, Genetic; Xenopus

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Full-Text Articles in Molecular Biology

Temporal Uncoupling Of The Dna Methylome And Transcriptional Repression During Embryogenesis, Ozren Bogdanovic, Steven W. Long, Simon J. Van Heeringen, Arie B. Brinkman, Jose Luis Gomez-Skarmeta, Hendrik G. Stunnenberg, Peter L. Jones, Gert Jan C. Veenstra Aug 2011

Temporal Uncoupling Of The Dna Methylome And Transcriptional Repression During Embryogenesis, Ozren Bogdanovic, Steven W. Long, Simon J. Van Heeringen, Arie B. Brinkman, Jose Luis Gomez-Skarmeta, Hendrik G. Stunnenberg, Peter L. Jones, Gert Jan C. Veenstra

Peter Jones Lab Publications

DNA methylation is a tightly regulated epigenetic mark associated with transcriptional repression. Next-generation sequencing of purified methylated DNA obtained from early Xenopus tropicalis embryos demonstrates that this genome is heavily methylated during blastula and gastrula stages. Although DNA methylation is largely absent from transcriptional start sites marked with histone H3 lysine 4 trimethylation (H3K4me3), we find both promoters and gene bodies of active genes robustly methylated. In contrast, DNA methylation is absent in large H3K27me3 domains, indicating that these two repression pathways have different roles. Comparison with chromatin state maps of human ES cells reveals strong conservation of epigenetic makeup ...