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Full-Text Articles in Molecular Biology

A Brain-Derived Mecp2 Complex Supports A Role For Mecp2 In Rna Processing, Steven W. Long, Jenny Y. Y. Ooi, Peter M. Yau, Peter L. Jones Oct 2011

A Brain-Derived Mecp2 Complex Supports A Role For Mecp2 In Rna Processing, Steven W. Long, Jenny Y. Y. Ooi, Peter M. Yau, Peter L. Jones

Peter Jones Lab Publications

Mutations in MECP2 (methyl-CpG-binding protein 2) are linked to the severe postnatal neurodevelopmental disorder RTT (Rett syndrome). MeCP2 was originally characterized as a transcriptional repressor that preferentially bound methylated DNA; however, recent results indicate MeCP2 is a multifunctional protein. MeCP2 binding is now associated with certain expressed genes and involved in nuclear organization as well, indicating that its gene regulatory function is context-dependent. In addition, MeCP2 is proposed to regulate mRNA splicing and a mouse model for RTT shows aberrant mRNA splicing. To further understand MeCP2 and potential roles in RTT pathogenesis, we have employed a biochemical approach to identify ...


Temporal Uncoupling Of The Dna Methylome And Transcriptional Repression During Embryogenesis, Ozren Bogdanovic, Steven W. Long, Simon J. Van Heeringen, Arie B. Brinkman, Jose Luis Gomez-Skarmeta, Hendrik G. Stunnenberg, Peter L. Jones, Gert Jan C. Veenstra Aug 2011

Temporal Uncoupling Of The Dna Methylome And Transcriptional Repression During Embryogenesis, Ozren Bogdanovic, Steven W. Long, Simon J. Van Heeringen, Arie B. Brinkman, Jose Luis Gomez-Skarmeta, Hendrik G. Stunnenberg, Peter L. Jones, Gert Jan C. Veenstra

Peter Jones Lab Publications

DNA methylation is a tightly regulated epigenetic mark associated with transcriptional repression. Next-generation sequencing of purified methylated DNA obtained from early Xenopus tropicalis embryos demonstrates that this genome is heavily methylated during blastula and gastrula stages. Although DNA methylation is largely absent from transcriptional start sites marked with histone H3 lysine 4 trimethylation (H3K4me3), we find both promoters and gene bodies of active genes robustly methylated. In contrast, DNA methylation is absent in large H3K27me3 domains, indicating that these two repression pathways have different roles. Comparison with chromatin state maps of human ES cells reveals strong conservation of epigenetic makeup ...