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Molecular Biology Commons

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Developmental Biology

University of Massachusetts Medical School

Peter Jones Lab Publications

2007

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Full-Text Articles in Molecular Biology

The Tripartite Motif (Trim) Of Nuclear Factor 7 Is Required For Its Association With Transcription Units, Brent Beenders, Peter L. Jones, Michel Bellini Apr 2007

The Tripartite Motif (Trim) Of Nuclear Factor 7 Is Required For Its Association With Transcription Units, Brent Beenders, Peter L. Jones, Michel Bellini

Peter Jones Lab Publications

In amphibian oocytes, the maternal nuclear factor NF7 associates with the elongating pre-mRNAs present on the numerous lateral loops of the lampbrush chromosomes. Here, we have purified NF7 from an oocyte extract by using a combination of ion-exchange chromatography and gel filtration chromatography and demonstrated for the first time that nucleoplasmic NF7 exists primarily as free homotrimers. We confirmed the in vivo homotrimerization of NF7 by using a glutaraldehyde cross-linking assay, and we further showed that it only requires the coiled-coil domain of the NF7 tripartite motif/RBCC motif. Interestingly, we also obtained evidence that NF7 is recruited to the ...