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Full-Text Articles in Molecular Biology
Design And Development Of A Plasmid Vector For Protein Expression And Purification, Mahima Grover, Craig Sweet, David H. Thompson
Design And Development Of A Plasmid Vector For Protein Expression And Purification, Mahima Grover, Craig Sweet, David H. Thompson
The Summer Undergraduate Research Fellowship (SURF) Symposium
Production and isolation of proteins are difficult, costly and time-consuming processes. The aim of this project is for the development of plasmids, which allow for streamlined production and isolation of proteins. To allow for modular insertion of varying segments of DNA we are using ‘recursive directional ligation by plasmid reconstruction’. This technique uses type II restriction endonucleases, which cut downstream from their recognition site allowing multiple insertions without losing a restriction site. Using this process, we can ligate multiple DNA sequences together and express them to be able to construct a scar less fusion protein. In order to accomplish this, …
Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins
Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.