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Examination Of Promotor Hypermethylation Patterns In Magnetically Enriched Exfoliated Breast Milk Epithelial Cells, Chung M. Wong
Examination Of Promotor Hypermethylation Patterns In Magnetically Enriched Exfoliated Breast Milk Epithelial Cells, Chung M. Wong
Masters Theses 1911 - February 2014
Suppression of genes involved in DNA repair, tumor suppression and detoxification through epigenetic modifications has been implicated in the etiology of cancer. As such analysis of promoter methylation patterns in genes frequently down regulated in breast cancer in non-cancerous subjects may serve as an indicator of breast cancer risk. CpG-island hypermethylation of single genes has been detected in cells isolated from nipple aspirate and ductal lavage, yet both isolation methods yield insufficient cells to complete an extensive analysis on any one donor sample.
As an alternative we have turned to magnetic separation of human mammary epithelial cells from breast milk. …