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Full-Text Articles in Molecular Biology

Rapid Molecular Detection And Population Genetics Of Pityophthorus Juglandis, A Vector Of Thousand Cankers Disease In Juglans Spp., Emel Oren Dec 2016

Rapid Molecular Detection And Population Genetics Of Pityophthorus Juglandis, A Vector Of Thousand Cankers Disease In Juglans Spp., Emel Oren

Masters Theses

Thousand Cankers Disease (TCD) is a disease complex involving the fungal pathogen Geosmithia morbida, an insect vector Pityophthorus juglandis, and the hosts, Juglans spp. and Pterocarya spp. Signs and symptoms of TCD include crown thinning due to branch dieback, yellowing and wilting of the leaves, appearance of epicormic shoots, numerous entrance/exit holes, gallery formation by P. juglandis, and the development of small, dark brown cankers underneath the bark. TCD originally described from western U.S., has now expanded to eastern U.S. and northwestern Italy. The disease complex is often difficult to diagnose due to the absence of symptoms ...


Promoting Extracellular Matrix Crosslinking In Synthetic Hydrogels, Marcos M. Manganare Jan 2015

Promoting Extracellular Matrix Crosslinking In Synthetic Hydrogels, Marcos M. Manganare

Masters Theses

The extracellular matrix (ECM) provides mechanical and biochemical support to tissues and cells. It is crucial for cell attachment, differentiation, and migration, as well as for ailment-associated processes such as angiogenesis, metastases and cancer development. An approach to study these phenomena is through emulation of the ECM by synthetic gels constructed of natural polymers, such as collagen and fibronectin, or simple but tunable materials such as poly(ethylene glycol) (PEG) crosslinked with short peptide sequences susceptible to digestion by metalloproteases and cell-binding domains. Our lab uses PEG gels to study cell behavior in three dimensions (3D). Although this system fosters ...


Engineering Camelina Sativa For Biofuel Production Via Increasing Oil Yield And Tolerance To Abiotic Stresses, Kenny Ablordeppey Jan 2014

Engineering Camelina Sativa For Biofuel Production Via Increasing Oil Yield And Tolerance To Abiotic Stresses, Kenny Ablordeppey

Masters Theses

In an effort to engineer Camelina sativa for enhanced oil yield and tolerance to abiotic stresses, we have cloned and overexpressed Camelina γ-Glutamyl Cyclotransferase (GGCT2;1); a gene involved in oxidative stress tolerance via glutathione homeostasis and Wrinkled 1; a transcription factor that regulates genes involved in fatty acid biosynthesis to increase triacylglycerol (TAG) accumulation in seeds. The GGCT gene family in Camelina consists of three genes-GGCT1, GGCT2;1 and GGCT2;2. Camelina GGCT genes showed differential expression under oxidative stress caused as a result of exposure to various abiotic stresses. The GGCT2;1 gene, which showed strong up-regulation ...


Bioconfinement Of A Putatively Sterile Nicotiana Hybrid And Development Of Tools For Assessing Gene Flow, John Hollis Rice Aug 2013

Bioconfinement Of A Putatively Sterile Nicotiana Hybrid And Development Of Tools For Assessing Gene Flow, John Hollis Rice

Masters Theses

Production of transgenic crops in open field environments is an ongoing concern of due to the potential for gene flow. New transgenic crops, such as plant-made-pharmaceuticals may generate additional concerns about effects of adventitious transgenes. Use of a bioconfinement strategy may alleviate any consequences by preventing gene flow. The following chapters discuss previous and current research on gene flow, testing of a Nicotiana hybrid system for bioconfinement efficiency, and development of methods for transgene detection. The candidate ‘platform plant’ that was tested is a Nicotiana hybrid (Nicotiana tabacum ‘TN 90’ × Nicotiana glauca) previously identified to be sexually sterile. To quantify ...