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Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Sandip S. Magdum
Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 …