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Full-Text Articles in Dairy Science

Extracellular Atp Effects On Intracellular Actin Fibrils' Location And Characteristics [Poster], Dianna Huisman, David Mcclenahan, Ali Tabei, Joseph Tibbs Apr 2018

Extracellular Atp Effects On Intracellular Actin Fibrils' Location And Characteristics [Poster], Dianna Huisman, David Mcclenahan, Ali Tabei, Joseph Tibbs

Research in the Capitol

Epithelial cells lining secretory units and ducts of bovine mammary glands perform an important role in regulating movement of various macromolecules and whole cells during normal lactation and mastitis. During mastitis, host and bacterial produced substances can affect the “barrier” function of epithelial monolayers. One potential component is adenosine triphosphate (ATP). ATP likely interacts with P2X7, a purinergic receptor, in mediating some effects associated with mastitis. Bovine mammary gland epithelial cell line, Mac-T cells, were examined for cytoskeletal changes as result of P2X7 interactions. Actin cytoskeletons were stained with phalloidin and effects were examined by fluorescent microscopy. Observable increase in …


Effect Of Extracellular Atp On Cellular Actin Fibrils' Location And Characteristics, Dianna Huisman Jan 2018

Effect Of Extracellular Atp On Cellular Actin Fibrils' Location And Characteristics, Dianna Huisman

Honors Program Theses

Epithelial cells lining secretory units and ducts of bovine mammary glands perform an important role in regulating movement of various macromolecules and whole cells during normal lactation and mastitis. During mastitis, host-and bacteria-produced substances can affect the “barrier” function of epithelial monolayers. One potential component is adenosine triphosphate (ATP). ATP likely interacts with P2X7, a purinergic receptor, in mediating some effects associated with mastitis including changes in cell permeability. The bovine mammary gland epithelial cell line, Mac-T cells, were examined for cytoskeletal changes as result of P2X7 interactions. Actin cytoskeletons were stained with phalloidin and effects were examined by fluorescent …