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Full-Text Articles in Aquaculture and Fisheries
Abstracts Of Technical Papers Presented At The 100th Annual Meeting National Shellfisheries Association Providence, Rhode Island April 6–10, 2008, National Shellfisheries Association
Abstracts Of Technical Papers Presented At The 100th Annual Meeting National Shellfisheries Association Providence, Rhode Island April 6–10, 2008, National Shellfisheries Association
VIMS Articles
No abstract provided.
Quantifying Fecundity In Macoma Balthica Using An Enzyme-Linked Immunosorbent Assay (Elisa), Wc Long, E Bromage, Rochelle D. Seitz, S Kaattari
Quantifying Fecundity In Macoma Balthica Using An Enzyme-Linked Immunosorbent Assay (Elisa), Wc Long, E Bromage, Rochelle D. Seitz, S Kaattari
VIMS Articles
Monoclonal antibodies specific to a heat shock protein 70-like protein expressed in the eggs of Macoma balthica (mb-HSP70) were employed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify fecundity in females. The assay was specific to egg tissue, with no demonstrable reactivity with juvenile or male tissues. The concentration of mb-HSP70 increased as the female gonads matured, necessitating the determination of a calibration curve for future experiments. The number of eggs in females was positively correlated with body mass index (BMI), and clams with a BMI < 1.4 did not produce eggs. The estimated number of eggs per clam during the fall spawning period was similar to that observed in eastern North Atlantic populations in clams of similar size; clams with shell lengths from 16 to 18 mm had from 7000 to 60 000 eggs apiece. Larger clams had up to 450 000 eggs. This assay effectively quantifies eggs at any stage of gonadal development in which eggs can be distinguished microscopically and is easier and cheaper to perform than other techniques of comparable precision.
Influence Of Sociality On Allometric Growth And Morphological Differentiation In Sponge-Dwelling Alpheid Shrimp, E Toth, Je Duffy
Influence Of Sociality On Allometric Growth And Morphological Differentiation In Sponge-Dwelling Alpheid Shrimp, E Toth, Je Duffy
VIMS Articles
Eusocial societies are defined by a reproductive division of labour between breeders and nonbreeders that is often accompanied by morphological differentiation. Some eusocial taxa are further characterized by a subdivision of tasks among nonbreeders, often resulting in morphological differentiation among different groups (subcastes) that specialize on different sets of tasks. We investigated the possibility of morphological castes in eusocial shrimp colonies (Zuzalpheus, formerly part of Synalpheus) by comparing growth allometry and body proportions of three eusocial shrimp species with three pair-forming species (species where reproductive females and males occur in equal sex ratios). Allometry of eusocial species differed in several …
Photosynthetic Maximum Quantum Yield Increases Are An Essential Component Of The Southern Ocean Phytoplankton Response To Iron, Mr Hiscock, Vp Lance, Am Apprill, Rr Bidigare, Zi Johnson, Walker O. Smith Jr., Et Al.
Photosynthetic Maximum Quantum Yield Increases Are An Essential Component Of The Southern Ocean Phytoplankton Response To Iron, Mr Hiscock, Vp Lance, Am Apprill, Rr Bidigare, Zi Johnson, Walker O. Smith Jr., Et Al.
VIMS Articles
It is well established that an increase in iron supply causes an increase in total oceanic primary production in many regions, but the physiological mechanism driving the observed increases has not been clearly identified. The Southern Ocean iron enrichment experiment, an iron fertilization experiment in the waters closest to Antarctica, resulted in a 9-fold increase in chlorophyll (Chl) concentration and a 5-fold increase in integrated primary production. Upon iron addition, the maximum quantum yield of photosynthesis (phi(m)) rapidly doubled, from 0.011 to 0.025 mol C.mol quanta(-1). Paradoxically, this increase in light-limited productivity was not accompanied by a significant increase in …
Co(2) Sensitivity Of Southern Ocean Phytoplankton, Pd Tortell, Cd Payne, Yy Li, S Trimborn, B Rost, Walker O. Smith Jr., Et Al.
Co(2) Sensitivity Of Southern Ocean Phytoplankton, Pd Tortell, Cd Payne, Yy Li, S Trimborn, B Rost, Walker O. Smith Jr., Et Al.
VIMS Articles
The Southern Ocean exerts a strong impact on marine biogeochemical cycles and global air-sea CO(2) fluxes. Over the coming century, large increases in surface ocean CO(2) levels, combined with increased upper water column temperatures and stratification, are expected to diminish Southern Ocean CO(2) uptake. These effects could be significantly modulated by concomitant CO(2)-dependent changes in the region's biological carbon pump. Here we show that CO(2) concentrations affect the physiology, growth and species composition of phytoplankton assemblages in the Ross Sea, Antarctica. Field results from in situ sampling and ship-board incubation experiments demonstrate that inorganic carbon uptake, steady-state productivity and diatom …
Biomarkers Of Oogenesis In Macoma Balthica Determined By Subtractive Immunization, E Bromage, Wc Long, S Kaattari
Biomarkers Of Oogenesis In Macoma Balthica Determined By Subtractive Immunization, E Bromage, Wc Long, S Kaattari
VIMS Articles
We employed the technique of subtractive immunization (SI) to identify specific biomarkers of oocyte development in Macoma balthica. Analysis revealed that 2 novel proteins (45 and 96 kDa) were induced during female gametogenesis that were neither present during other life stages, nor in male clam tissues. A total of 78 viable hybridomas were specific for either the 45 or 96 kD M balthica proteins. Subsequent peptide fragment analysis of the 96 kDa protein revealed that it was essentially identical (>99.9%) to the evolutionarily conserved heat shock protein 70 family. The resultant hybridomas were screened for their utility in a …