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Metabolic Support Of Anaerobiosis In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Andrew Mccracken
Metabolic Support Of Anaerobiosis In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Andrew Mccracken
Dissertations and Theses
Embryos of the annual killifish Austrofundulus limnaeus display a remarkable tolerance to anoxia during development, most notably during embryonic diapause. Little is known about the metabolic or enzymatic changes that accompany this state of anoxia tolerance. This study examined the metabolic changes associated with exposure to anoxia by measuring the activity of the enzyme phosphoenolpyruvate carboxykinase (PEPCK), and by profiling the concentration of 31 metabolites ranging from amino acids to citric cycle intermediates at 4 different developmental stages, diapause 2 (DII), 4 days post diapause (dpd), 12 and 22 dpd. Embryos of A. limnaeus showed stage specific changes in concentrations …