Agriculture Commons^™

Effects Of Wilting Extent On The Phytoestrogen Levels, Nutritional Value, Microbial Populations, And In Vitro Ruminal Methane Emissions Of Red Clover Hay And Silage Across Stages, Diego Zamudio Ayala

Electronic Theses and Dissertations

The main objective of this thesis is to improve the understanding and awareness of methodologies to decrease phytoestrogens in conserved legumes without sacrificing forage nutritive value. In chapter 1, we discussed the main factors influencing each stage of hay production and our current understanding of the hay microbiome dynamics. The primary objective of haymaking is to dry forage enough (80-85% DM) to inhibit the growth of undesirable microbes and halt residual plant enzymatic activity that causes nutrient losses. During the field and storage phases of haymaking, the environment, management practices, and other factors influence the extent of DM losses. This …

Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler

Electronic Theses and Dissertations

Animals have trillions of microorganisms living in or on many body sites, these communities of microorganisms are called microbiomes. Microbiomes are typically host-specific, and a lot of information about the host can be determined from investigating them. Microbiome research has many real-world applications, and this thesis utilizes the One Health perspective, which acknowledges the connection of humans, animals, and environments, and emphasizes the need for collaborative, interdisciplinary research. The first interdisciplinary project is an investigation into the bacteria in wild and cultured Atlantic deep-sea scallop, Placopecten magellanicus larvae. Adults in hatcheries can be induced to spawn, but the last two …

To Know The Land With Hands And Minds: Negotiating Agricultural Knowledge In Late-Nineteenth-Century New England And Westphalia, Justus Hillebrand

Electronic Theses and Dissertations

Ever since the eighteenth century, experts have tried to tell farmers how to farm. The agricultural enlightenment in Europe marked the beginning of a long arc of new experts aiming to change agricultural knowledge and practice. This dissertation analyzes the pivotal period in the late nineteenth and early twentieth century in Germany and the United States when scientists, improvers, and market agents began to develop comprehensive ways to communicate agricultural innovation to farmers. In a functional approach to analyzing the negotiation of agricultural knowledge through its communication in things, words, and practices, this dissertation argues that the process of change …

Expanding Our Understanding On Feed Spoilage And Developing Novel Approaches For Its Mitigation, Marjorie A. Killerby

Electronic Theses and Dissertations

The main objective of this study is to improve the understanding and awareness of conserved feed spoilage and to develop novel technologies that can prevent it. In chapter 1, we conducted a meta-analysis to evaluate the effects of chemical (50 articles)and biological (21 articles)preservatives on hay spoilage during storage. Multi-level linear mixed-effects modelswere fit including moderators:Preservative Class (PC), forage type (FT); moisture class (MC), and application rate (AR). Dry matter (DM) loss was affected by PC×FT (P=0.045), PC×AR (P<0.001), and PC×MC (P=0.009),relative to the overall effect of preservatives (-0.37% units). DM loss in propionic acid (PropA)-treated hay was numerically reduced to a greater extent in grasses (-16.2), followed by mixed hay (-1.76), but it was actually increased (+2.2%)in legume hay.Increasing ARof PropA resulted in decrease in DM loss (β = -1.34).Application ofBuffered Organic Acids,Other Organic Acids, PropA,and Anhydrous Ammoniadecreased visual moldiness by -22.1, -29.4, -45.5 and -12.2% units, respectively (PC; P<0.001). Sugars increased in treated grass hay (+1.9) and decreased in treated legume hay -0.8% DM, respectively (P<0.001). Microbial inoculants had small effects on hay spoilage since the overall DM loss effect size was - 0.21%. Inoculated grass hay preserved moresugars (+1.47) than treated legume hay (+0.33) relative to untreated hay (x=4.63% DM; P<0.001). In conclusion, organic acid-based preservatives effectively prevent spoilage of hay as influenced by FT, MC and AR, but microbial inoculants had only small effects. In chapter 2, we evaluated the effects of chemical and biological preservatives and ensiling stage on spoilage, ruminal in vitro fermentation, and methane production of wet brewer’s grain (WBG) silage. Treatments (TRT) were sodium lignosulfonate at10 g/kg (NaL1) and 20 g/kg (NaL2; fresh WBG), propionic acid at 5 g/kg fresh WBG (PRP, 99%), a combination inoculant (INO; Lactococcus lactisand Lactobacillus buchneri each at 4.9 log cfu/fresh WBG g), and untreated (CON). Three stages (STG) were tested:treated WBG (Fresh) was ensiled for 60 d(Ensiled), after which they were opened and aerobically exposed for 10 d (AES). Data were analyzed as a RCBD (5 blocks) with a 5 TRT × 3 STG(Fresh, Ensiled and AES) factorial arrangement. Our results show that Ensiled PRP-treated WBG markedly preserved more sugars (250) and starch (190) than all other Ensiled TRT (x=136 ± 16.2sugarsg/kg DM and x=121 ± 6.15 starch g/kg DM, respectively; P<0.001). Due to its superior nutritive value, PRP-treated AES was less aerobically stable than CON (61.9 vs. 90.1 h; P = 0.03). Preservation was not improved by INO, NaL1 or NaL2, but the latter prevented the increase of neutral detergent fiber (NDF)across STG (x=384).In vitro ruminal fermentation of Fresh WBG resulted in higher methane concentration (0.94) and yield (0.27) than theother STG (x=0.84 ± 0.07mMand x=0.23 ± 0.03mmol/g fermented OM, respectively). In conclusion, PRP was the most effective at preserving WBG during ensiling but failed to improve aerobic stability. In chapter 3, we evaluated the effects of application rate (AR) of sodium lignosulfonate (NaL) and propionic acid(PRP)on high moisture alfalfa hay spoilage during storage and its nutritive value. Treatments (TRT; NaL and PRP) were applied at four AR: 0 (CON), 2.5, 5 and 10 g/kg (fresh basis) to alfalfa hay at 68.5% DM, packed into mini balesand stored for 33 d.Data were analyzed as a RCBD(5 blocks) with a 2 TRT x 4 AR factorial arrangement. At d 33, DM losses were decreased by PRP at 5 and 10 g/kg (0.9), vs.CON (6.92) andNaL (6.63 ± 1.13%). Visual moldiness (0-10) and mold counts were also decreased by PRP at 5 (2.4 and 5.30) and 10 g/kg (0 and 2.7) relative to CON (6.0 and 7.13) and NaL (5.85 ± 0.67 and 7.21 ± 0.31 log cfu/fresh g, respectively). Both TRT increased apparent in vitroDM digestibility at all AR relative to CON (x=543 vs 501 ± 12.0g/kgof DM, respectively), but the acetate-to-propionate ratio was higher for NaL at 10 g/kg (3.02) vs. CON (2.89 ± 0.04). In conclusion, NaL failed to prevent spoilage of high moisture alfalfa hay while PRP was effective at doses >5 g/kg. Both TRT at 10 g/kg improved the rumen in vitro fermentation measures of high moisture alfalfa hay but PRP …

Characterization Of The Rumen Bacterial Communities Of Bison Heifers Fed A Grass-Based Diet Vs A Grain-Based Free-Choice Diet, Anlly Miley Fresno Rueda

Electronic Theses and Dissertations

A century ago, the North American grasslands and prairie ecosystems were dominated by bison. At least 30 million bison roamed the Great Plains when the first explorers arrived. By 1900, there were little over a thousand bison remained in the United States and Canada. Recovery efforts has been made since the 20th century to reestablish the herds and increase the bison population. Today, over 500,000 bison are distributed across North America, with more than 90% of the existing bison population under commercial production. Modern conservation strategies are made via the collaborative efforts of conservationist, producers, and researchers, resulting in increased …

Management And Dietary Strategies To Mitigate Environmental Stressors In Northern Plains Cattle Feeding Systems, Warren Carl Rusche

Electronic Theses and Dissertations

Three studies were undertaken to examine strategies to mitigate environmental stress faced by Northern Plains cattle feeders. In the first study, 46 feedlot managers from SD (n = 21), MN (n = 6), and NE (n = 19) were surveyed on use and perceptions of heat stress (HS) mitigation strategies. All respondents avoided cattle handling during heat stress events and incorporated some method of HS mitigation. Buildings or shades were perceived as the most successful strategy (60.9%) and were most commonly named as strategies managers would like to employ (63% for shades and 17.4% for buildings). Increasing dietary corn silage …

Developing Whey Protein Encapsulated Probiotics And Evaluating Environmental Listeria Using Whole-Genome Sequencing, Shayanti Minj

Electronic Theses and Dissertations

In this study, a novel value-added dairy-based health formulation was developed using whey protein hydrolysate and probiotic organisms. In the first part of the study, different forms of whey proteins, concentrate WPC80, isolate WPI90, and hydrolysates WPH10, WPH15 and WPH20 were screened for bioactivities (antimicrobial, antioxidant, and antihypertensive activity). Hydrolysate WPH10, exhibiting the highest bioactivities was conjugated with maltodextrin. A batch of 2L conjugated solution was spray dried in a Niro drier with an inlet and outlet temperature of 200⁰C and 90⁰C, and alternatively, freeze dried at −80 °C under 50 mTorr vacuum. The bioactivities of the conjugated samples were …

Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez

Electronic Theses and Dissertations

This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …

Analyzing The Longevity Of Sperm Within The Female Japanese Quail By Assessing Sperm Penetration Of The Perivitelline Layer Under Optimal And Suboptimal Conditions., Garret G. Ashabranner, Joey L. Bray

Electronic Theses and Dissertations

After mating, birds have the ability to store semen within the female reproductive tract. The sperm storage tubules will store and subsequently release semen to travel up the oviduct. Sperm cells that make the trek up the oviduct have a chance to fertilize the ovum. These sperm cells will bind to the perivitelline layer of the ovum and hydrolyze a hole in the perivitelline layer, where it has the possibility to fertilize the female sex cell. Analyzing the number of penetration points on the perivitelline layer is an effective way to analyze reproductive efficiency. Many environmental factor has its effect …

Analyzing The Management Practices Of East Texas Beef Cattle Producers, Hiliary Cheyenne Swor

Electronic Theses and Dissertations

A survey of East Texas beef cattle producers was designed to evaluate the management practices that were prevalent in the area. Demographic and cattle operation data from 103 respondents were analyzed using SPSS (Version 25; Chicago, IL). This electronic-based survey was distributed through Qualtrics. Respondents were located in Angelina, Nacogdoches, or Cherokee county. Most producers (38.8%) had a Bachelor’s degree. A negative correlation was established between castration method and weaning weight (p = - 0.204). Calves were weaned 50 pounds heavier when band or surgically castrated. Females were less likely to castrate their calves or utilize a mineral program. Approximately, …

Optimization Of Conditions For Heat Pretreatment And Enzymatic Predigestion Of Ddgs For Pigs, Kevin Jerez-Bogota

Electronic Theses and Dissertations

The high fiber content of corn dried distillers’ grains with solubles (DDGS) can limit its utilization in swine diets. Pretreatment with heat and enzymatic predigestion of whole stillage (WS; slurry material that is dried into DDGS) can alleviate negative effects of dietary fiber and can improve digestive and fermentation characteristics of the feedstuff. However, optimal time and temperature of the heat pretreatment of WS, and best enzymes for predigestion of WS have not been identified. Experiments were conducted to identify optimal conditions for heat pretreatment and multienzyme predigestion of WS for pigs. First experiment was conducted to identify optimal temperature …

Understanding How Agricultural Intensification Impacts Ring-Necked Pheasant Distribution And Survival In Eastern South Dakota, Hilary R. Kauth

Electronic Theses and Dissertations

Historically, pheasants (Phasianus colchicus) have thrived in South Dakota in conjunction with successful land retirement programs or early farming practices through the 1950s, which created interspersions of agriculture and native landscapes that were ideal for pheasants. Recently, the Prairie Pothole ecosystem has undergone rapid agroeconomic expansion, effectively reducing ideal interspersions of native prairie and cropland into agriculturally homogeneous landscapes. Indices of pheasant abundance have suggested persistent population declines since 2008, raising concerns regarding landscape suitability. Our goal was to understand how agriculture intensification impacts pheasant ecology. The objectives were to: 1) estimate overwinter hen probability of survival, resource …

The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga

Electronic Theses and Dissertations

Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and …

Hydrodynamic Cavitation As In-Line Process To Control Common Dairy Sporeformers, Pratibha Chaudhary

Electronic Theses and Dissertations

Sporeforming bacteria and their endospores have the ability to survive thermal treatments and proliferate in the milk and dairy products to cause spoilages. We applied the effect of hydrodynamic cavitation in controlling these bacteria and their spores at pilot scale level. Our aim was to investigate a technology that would improve the microbial quality of milk and dairy products, and is capable of being scaled up to be adapted at industrial level. Cavitation damages the cell walls of the bacteria and their endospores (releasing dipicolinic acid, responsible for endospore resistance to extreme conditions), thereby making them susceptible to thermal treatments …

Evaluation Of Dietary Phytochemicals As Rumen Modifiers In Lactating Dairy Cows, Sonia Rodriguez Jimenez

Electronic Theses and Dissertations

Phytochemicals such as tannins included in dairy cow nutritional programs, as a natural feed additive, promise to improve rumen bypass protein and consequently reduce the feeding cost by decreasing the amount of CP needed in the ration. Additionally, if tannins are fed along with high quality protein sources, this could improve the AA profile reaching the small intestine and potentially improving health and performance of dairy cows. The black pepper, specifically its alkaloid piperine, is a nontoxic, natural dietary compound with a broad range of physiological activity. Lactating dairy cows ingesting these phytochemicals (tannin extracts and black pepper) will potentially …

Efficiency Of Utilizing Standardized Ileal Digestible Lys And Thr For Whole Body Protein Retention In Pregnant Gilts During Early, Mid And Late Gestation, Ron Aldwin Sapin Navales

Electronic Theses and Dissertations

In pregnant pigs, amino acid (AA) requirements represent the sum of those required for maintenance functions, protein retention and efficiency of utilizing AA intake for the aforementioned body processes. The NRC (2012) model assumed AA efficiency is constant across period of gestation; however this is not reflective of the changes in metabolic demand during gestation. Therefore, two experiments were conducted to evaluate the efficiency of utilizing SID Lys and Thr for whole body protein retention (kSIDLys and kSIDThr) in pregnant gilts during early, mid and late gestation. Three 12 d N-balance studies were conducted to represent different periods of gestation. …

Can Solvent-Or Mechanically-Extracted Carinata Meal Be Used As An Effective Source Of Supplemental Protein To Cows Fed Poor Quality Forages?, Emily Jacklyn Rosenthal

Electronic Theses and Dissertations

Brassica carinata is an oilseed crop that requires a relatively short growing season and produces high crop yields. It is a great source for aviation biofuel, however it produces a large amount of waste that may be used as a protein supplement for cattle. We tested the effects of solvent- or mechanically-extracted carinata meal as a protein supplement to ad libitum ground or long-stem corn residue (i.e., corn plant left over from grain harvest) had on cow performance and digestibility. Fifty-six non-pregnant cows in 8 pens had ad libitum access to either long-stem or ground corn residue in addition to …

Evaluation Of Absorbezz On Swine Reproductive Health And A Comparative Study Of Two Commercially Available Swine Show Feeds, Scott G. Sutton

Electronic Theses and Dissertations

The efficacy of a feed supplement fed to gilts and sows was tested in study I. The supplement was stated to improve reproductive soundness, conception rates, immune health, and overall well-being of livestock. Thirty gilts were split into a control group (Base diet) and a treatment group (Absorbezz® 0.32 mL on base diet). Upon completion of the trial results indicate the treatment group significantly underperformed (

Two commercially available show pig growing rations were tested in study II to find a high quality, cost efficient product. A total of 10 pigs (5 barrows, 5 gilts), at an average weight of …

Evaluation Of Different Probiotic Strains Supplemented In Commercial Broiler Rations And Their Influences On Performance, Yield, And Intestinal Microbiota., Justin M. Glasscock

Electronic Theses and Dissertations

The objective of this study was to evaluate Bacillus megaterium, Bacillus licheniformis, and Bacillus subtilis, as probiotic strains compared to Bacitracin Methylene Disalicylate (BMD) 50 as the antibiotic growth promotor, supplemented in commercial broiler rations and their influences on performance, yield, and intestinal microbiota. This trial was completed as a randomized-block design with 4,800 birds split into 96, 5’x10’ pens, and randomly assigned to one of eight treatment groups. The birds were placed at a stocking density of 1.00 ft²/bird, (50 birds/pen), and reared on used pine shaving for 55 days. Throughout the study, bird …

Local And Systemic Actions Of Gnrh Relating To Estradiol And Associated Reproductive Parameters In Beef Cattle, Jerica J.J. Rich

Electronic Theses and Dissertations

In cattle, estradiol is responsible for modulating many mechanisms involved in successful reproduction. Specifically estradiol is the primary signal for the initiation of standing estrus (Allrich, 1994), and cattle expressing estrus prior to fixed time AI (FTAI) have been reported to have increased preovulatory estradiol concentrations (Perry and Perry, 2008) and increased pregnancy success compared to animals that did not exhibit estrus (Richardson et al., 2016). Gonadotropin releasing hormone, classically, stimulates estradiol production via the two-cell two-gonadotropin hypothesis (Fortune and Quirk, 1988), and GnRH administered systemically in small doses (5 μg) has been reported to elicit an LH pulse similar …

Social Ecological Food Systems: Sustainability Lessons From Maine Dairy Networks, Julia B. Mcguire

Electronic Theses and Dissertations

Milk production has played an integral role in the culture, landscape, and economy of Maine’s agriculture. Maine dairy farmers have faced numerous sustainability challenges to economic, environmental, and social aspects of their industry. Like many other complex social ecological systems, the Maine dairy industry faces a gap between scientific knowledge and actionable management or policy. A cultural dichotomy exists between conventional and organic farming. Shifting the focus from this binary, metrics such as social capital may play a key role in solving sustainability issues. Difficulties arise in the governance of complex social ecological systems when the scales of assessment, management, …

Study Of The Effect Of Feeding Highly Digestible Corn Based Calf Starter On The Growth Performance Of Calves, Shruti Srivastava

Electronic Theses and Dissertations

New corn hybrids have been developed by Masters Choice (MC) that vary in energy density and starch digestibility. These MC hybrids have lower starch densities due to an altered starch structure, which allows for greater ruminal and intestinal starch digestion. The altered starch structure of these new hybrids results in reduced feed manufacturing costs (i.e., grinding). Thirty (30) 1 to 3 d old (40.6 ± 1.72 kg) Holstein heifer calves were randomly assigned to 1 of 2 calf starters to measure growth performance of Holstein heifer calves through 8 weeks of age. Treatments were: 1) Hard starch (HS) corn calf …

Response Of Soil And Water Quality To Winter Manure Application From Small Agricultural Watersheds In South Dakota, Shikha Singh

Electronic Theses and Dissertations

The extreme winter conditions prevailing in the state of South Dakota make it difficult for the livestock producers to manage the manure generated at the farm. The South Dakota Department of Environmental and Natural Resources does not recommend manure application in the state during the winter months when the ground is frozen. Thus, producers are left with the options such as storing the manure over a longer period until summer or spreading on snow or frozen ground. Storing manure for longer duration leads to increased risks of concentrated spills into the streams. Thus, it is important to develop management strategies …

Crop/Livestock Integration Effects On Soil Quality, Crop Production, And Soil Nitrogen Dynamics, Ellen B. Mallory

Electronic Theses and Dissertations

Regional integration of potato and dairy farms has developed in Maine through arrangements where manure, feed, and sometimes land, are exchanged between neighboring farms. The effects of integration on soil quality, crop production, nitrogen (N) cycling, and N loss were investigated in field and laboratory studies of contrasting amended (manure, compost, green manure, and supplemental fertilizer) and nonamended (fertilizer only) soil management systems within 2-year potato (Solanum tuberosum L.) rotations in the Maine Potato Cropping Systems Project (MPEP). Additionally, soil quality of 48 integrated and nonintegrated Maine potato and dairy farm fields was assessed. The MPEP’s amended soil system enhanced …