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Full-Text Articles in Life Sciences
Cold Adaption Behaviors Of Vibrio Vulnificus And Vibio Parahaemolyticus In Oysters, Chiung-Ta Wu
Cold Adaption Behaviors Of Vibrio Vulnificus And Vibio Parahaemolyticus In Oysters, Chiung-Ta Wu
LSU Master's Theses
Vibrio vulnificus is an estuarine bacterium that can cause primary septicemia as well as other serious wound infections. Vibrio parahaemolyticus is a gram-negative, halophilic bacterium that may cause gastroenteritis, diarrhea, headache, vomiting, nausea, abdominal cramps and is the leading cause of gastroenteritis in United States. Consumption of raw oysters is believed to closely relate with infection with Vibrio vulnificus and Vibrio parahaemolyticus. All strains of V. parahaemolyticus produce tlh genes and the major virulence factors of V.parahaemolyticus are the tdh and trh genes. This study was conducted to determine if Vibrio vulnificus and Vibrio parahaemolyticus clinical and environmental strains display …
Detection Of Vibrio Vulnificus By Direct Colony Immunoblot, Reshani Nisansala Senevirathne
Detection Of Vibrio Vulnificus By Direct Colony Immunoblot, Reshani Nisansala Senevirathne
LSU Master's Theses
Vibrio vulnificus (Vv) is a natural occurring bacterium of world wide estuarine environments, which concentrate on filter feeding shellfish. Under cooked seafood contaminated with this pathogen is a leading cause of 95% of seafood-related, foodborne deaths. The development of a rapid, reliable and user-friendly method for Vv enumeration would help to reduce mortality rate. A direct colony immunoblot (DCI) method was developed and used as a rapid enumeration with high sensitivity and the specificity. This method was optimized using Vv agar plates incubated for 16 h at 35 °C. Colonies were transferred from incubated plates to polyvinylidene fluoride (PVDF) membranes …