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Full-Text Articles in Life Sciences
Natural Variation And Evolutionary Responses To Climate Change Stressors In Marine Invertebrates, Joanna Sarah Griffiths
Natural Variation And Evolutionary Responses To Climate Change Stressors In Marine Invertebrates, Joanna Sarah Griffiths
LSU Doctoral Dissertations
Our rapidly changing climate is putting many species at risk of extinction and there is an urgent need to understand how species will respond to these changes. In this dissertation, I evaluate how three species of marine invertebrates (corals, oysters, and copepods) respond to stressful conditions in their current environments and how plasticity and evolutionary adaptation could alter their response to future climate change stressors. I first employed a space for time study to elucidate population differences in the response of cold-water corals, Balanophyllia elegans, to future ocean acidification. I found evidence that upwelling history (natural low pH exposure) influences …
Antimicrobial Properties Of Different Chitosans Against Gram-Negative And Gram-Positive Foodborne Pathogens In Food Products, Nancy Katherine Rubio Zapata
Antimicrobial Properties Of Different Chitosans Against Gram-Negative And Gram-Positive Foodborne Pathogens In Food Products, Nancy Katherine Rubio Zapata
LSU Doctoral Dissertations
Chitosan is a polymer derived of the deacetylation of chitin that is one of the most abundant material in nature. Chitosan solutions applied as edible coatings, and dipping solutions have shown positive results in the extension of shelf life on seafood and meat products. The purpose of this study was to evaluate the antimicrobial activities of a newly invented high molecular weight water-soluble (HMWWS) chitosan against selected Gram-negative (Salmonella Typhimurium, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae) and Gram-positive (Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) foodborne pathogens (initial inoculation of 6.5 Log). Chitosans with …
Human Norovirus In Artificial And Environmental Marine Water: Development Of Antibody Based Rapid Methods, Morgan Maite
Human Norovirus In Artificial And Environmental Marine Water: Development Of Antibody Based Rapid Methods, Morgan Maite
LSU Doctoral Dissertations
Norovirus (NoV) is the principal cause of viral gastroenteritis in the United States. It has been linked to filter-feeding molluscan shellfish, that bioaccumulate the virus from contaminated surrounding waters. The consumption of raw or undercooked contaminated oysters may result in acute gastroenteritis. We investigated the occurrence of NoV GI and GII and microbial indicators of fecal contamination in oysters and harvesting water from areas along the Louisiana Gulf Coast. We developed a filtration and concentration method for the detection of NoV from oyster harvesting waters. Lastly, this body of work compares commonly used molecular techniques (RT-PCR) and a commercial enzyme …
Development Of Safe And Ready To Eat Frozen Oyster Products Using Microwave Steam-Venting Technology, Luis Alberto Espinoza
Development Of Safe And Ready To Eat Frozen Oyster Products Using Microwave Steam-Venting Technology, Luis Alberto Espinoza
LSU Doctoral Dissertations
The Gulf Coast region, principally Louisiana, leads the nation in oyster production. The National Marine Fisheries Service reported that 19.7 million pounds of oyster meat, valued at $62.3 million dollars were harvested from the Gulf Coast region in 2010. One of the main concerns for this industry is the health risk associated with the consumption of oysters, particularly for at-risk populations. Oysters are filter feeders. They tend to concentrate microbes present in surrounding waters, some of which can cause severe illness in susceptible humans. Among pathogens that contaminate the gastrointestinal system of oysters, Vibrio spp (especially V. parahaemolyticus and V. …
Antimicrobial Susceptibility, Genotypic Characterization, And Molecular Detection Of Vibrio Parahaemolyticus And Vibrio Vulnificus From Louisiana Oysters, Feifei Han
LSU Doctoral Dissertations
Members of the genus Vibrio are Gram-negative, halophilic bacteria that inhabit warm coastal and estuarine waters worldwide. Among pathogenic vibrios, Vibrio parahaemolyticus is the leading cause of seafood-related illnesses and Vibrio vulnificus causes the highest number of seafood-related deaths in the United States. Moreover, according to the U.S. Centers for Disease Control and Prevention, the incidence of infections of the two vibrios due to the consumption of oysters has shown a sustained increase since 2001, indicating further measures are needed to prevent human Vibrio illness. In this dissertation research, a total of 622 Vibrio isolates, consisting of 252 V. parahaemolyticus …
Control Of Vibrio Vulnificus And Vibrio Parahaemolyticus In Oysters, Ligia Virginia Antonia Da Silva
Control Of Vibrio Vulnificus And Vibrio Parahaemolyticus In Oysters, Ligia Virginia Antonia Da Silva
LSU Doctoral Dissertations
Vibrio vulnificus and Vibrio parahaemolyticus are gram-negative halophilic bacteria found in the natural aquatic environment. V. vulnifcus and V. parahaemolyticus have been implicated in foodborne illness and can cause gastroenteritis that has been associated with consumption of raw or undercooked seafood. V. vulnificus can cause primary septicemia after its ingestion, and secondary septicemia through skin lesions in individuals with preexisting conditions such as elevated serum iron levels. Bacteriophages, viruses that invade and lyse bacteria, specific to V. vulnificus and V. parahaemolyticus are naturally found in seawater and oysters. Every summer, the oyster industry is threatened by recall of oysters due …