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Full-Text Articles in Life Sciences

Absence Of A Structural Basis For Intracellular Recognition And Differential Localization Of Nuclear And Plasma Membrane-Associated Forms Of Simian Virus 40 Large Tumor Antigen., Donald L. Jarvis, Charles N. Cole, Janet S. Butel Mar 1986

Absence Of A Structural Basis For Intracellular Recognition And Differential Localization Of Nuclear And Plasma Membrane-Associated Forms Of Simian Virus 40 Large Tumor Antigen., Donald L. Jarvis, Charles N. Cole, Janet S. Butel

Dartmouth Scholarship

The simian virus 40 large tumor antigen (T-ag) is found in both the nuclei (nT-ag) and plasma membranes (mT-ag) of simian virus 40-infected or -transformed cells. It is not known how newly synthesized T-ag molecules are recognized, sorted, and transported to their ultimate subcellular destinations. One possibility is that these events depend upon structural differences between nT-ag and mT-ag. To test this possibility, we compared the structures of nT-ag and mT-ag from simian virus 40-infected cells. No differences between the two forms of T-ag were detected by migration in polyacrylamide gels, by Staphylococcus aureus V8 partial proteolytic mapping of methionine- …


Inheritance Of Leucoanthocyanidin Content In Sorghum Leaves, Francis A. Haskins, Herman J. Gorz Mar 1986

Inheritance Of Leucoanthocyanidin Content In Sorghum Leaves, Francis A. Haskins, Herman J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Leucoanthocyanidins (LAC) are structurally related to condensed tannins, a class of compounds having significant effects on the nutritional value of sorghum [Sorghum bicolor (L.) Moench) grain. Little is known of LAC inheritance in sorghum. Mature leaves of 'Colman' and 'White Collier' forage sorghum are high and low, respectively, in LAC content. The objective of the present study was to determine the inheritance of LAC content in reciprocal crosses between these two cultivars, Leaves of field-grown plants were assayed for LAC by a procedure that involved treating methanolic leaf extracts with acid at 50°C to convert the colorless LAC to …


Inheritance Of Dhurrin Content In Mature Sorghum Leaves, Herman J. Gorz, Francis A. Haskins, K. P. Vogel Jan 1986

Inheritance Of Dhurrin Content In Mature Sorghum Leaves, Herman J. Gorz, Francis A. Haskins, K. P. Vogel

Department of Agronomy and Horticulture: Faculty Publications

Seedlings of both KS8 and N32 sorghum [Sorghum bicolor (L.) Moench] were high in dhurrin [p-hydroxy-(S)-mandelonitrile-β-D-glucoside] and thus in hydrocyanic acid potential (HCN-p), but the HCN-p of mature leaves from field-grown plants of KS8 was only about one-tenth as high as that of N32. A study of the inheritance of this large difference between KS8 and N32 revealed that a single major gene pair was responsible. There were no obvious maternal effects and F,'s were generally intermediate in HCN-p level between the two parents, indicating that neither high nor low HCN-p was completely dominant. HCN-p …