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Articles 31 - 60 of 290
Full-Text Articles in Life Sciences
Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens, Adam M. Baker, Carl T. Redmond, Stephen B. Malcolm, Daniel A. Potter
Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens, Adam M. Baker, Carl T. Redmond, Stephen B. Malcolm, Daniel A. Potter
Entomology Faculty Publications
Public interest in ecological landscaping and gardening is fueling a robust market for native plants. Most plants available to consumers through the horticulture trade are cultivated forms that have been selected for modified flowers or foliage, compactness, or other ornamental characteristics. Depending on their traits, some native plant cultivars seem to support pollinators, specialist insect folivores, and insect-based vertebrate food webs as effectively as native plant species, whereas others do not. There is particular need for information on whether native cultivars can be as effective as true or “wild-type” native species for supporting specialist native insects of conservation concern. Herein …
Data For Rosenberger & Conforti. Native And Agricultural Grassland Use By Stable And Declining Bumble Bees In Midwestern North America. Insect Cons. & Div., Derek W. Rosenberger, Mckenna Conforti
Data For Rosenberger & Conforti. Native And Agricultural Grassland Use By Stable And Declining Bumble Bees In Midwestern North America. Insect Cons. & Div., Derek W. Rosenberger, Mckenna Conforti
Faculty Scholarship – Biology
Little is known about how agricultural and native grasslands impact bumble bee populations in the Midwestern United States. We surveyed bumble bee populations over 3 years (2017-2019) at Midewin National Tallgrass Prairie in restored prairie, cattle and bison pastures. Raw data from that work is included in this file. These data were used in the manuscript "Native and agricultural grassland use by stable and declining bumble bees in Midwestern North America" by Derek Rosenberger and McKenna Conforti, and published in the journal Insect Conservation and Diversity in 2020.
Wireworms, Ryan Davis, Jay B. Karren, Alan H. Roe
Wireworms, Ryan Davis, Jay B. Karren, Alan H. Roe
All Current Publications
Wireworms are the larvae (immatures) of click beetles. Adults are elongate, slender, hardshelled beetles that take their name from their habit of flipping into the air (sometimes with an audible clicking sound) when they are placed on their backs. This fact sheet reviews the management of wireworms.
The Impacts Of Prescribed Burning And Mechanical Thinning On Insect Communities In The Arkansas Ozarks, Aaron P. Tormanen
The Impacts Of Prescribed Burning And Mechanical Thinning On Insect Communities In The Arkansas Ozarks, Aaron P. Tormanen
Theses and Dissertations from 2020
Insects are important in woodland ecosystems due to their role as pollinators and as prey for bats. My research investigated the relationships between forest management, vegetation, and insects in the Ozark National Forest in Arkansas. I selected 30 stands burned at varying frequencies in the last 12 years. Twelve of these stands were burned and mechanically thinned, 12 were only burned, and 6 were untreated. I deployed blacklight traps and malaise traps in each stand monthly from mid-March to mid- November 2019. Over 42,391 insects were collected, and Lepidoptera was the most abundant order. Insects were dried, weighed, and identified …
Current Applications And Limitations Of Forensic Entomology, Helena Volckaert
Current Applications And Limitations Of Forensic Entomology, Helena Volckaert
Themis: Research Journal of Justice Studies and Forensic Science
Forensic entomology is the study of arthropods concerning their application in crime scene investigation as a method of predicting postmortem interval (PMI) in deceased or missing individuals. PMI is determined by the life stage of insects present on-scene. Insects used in criminal investigations are primarily beetles, flies, or moths; this may also include various scavenging insects and hitchhiking mites. These creatures help colonize decayed tissue, providing crime scene personnel with live specimens collected and examined for biological evidence. Forensic entomology is currently a niche application in most investigations—as qualified entomologists are often needed—and proper analysis of collected evidence can be …
Recent Hybrids Recapitulate Ancient Hybrid Outcomes, Samridhi Chaturvedi, Lauren K. Lucas, C. Alex Buerkle, James A. Fordyce, Matthew L. Forister, Chris C. Nice, Zachariah Gompert
Recent Hybrids Recapitulate Ancient Hybrid Outcomes, Samridhi Chaturvedi, Lauren K. Lucas, C. Alex Buerkle, James A. Fordyce, Matthew L. Forister, Chris C. Nice, Zachariah Gompert
Ecology Center Publications
Genomic outcomes of hybridization depend on selection and recombination in hybrids. Whether these processes have similar effects on hybrid genome composition in contemporary hybrid zones versus ancient hybrid lineages is unknown. Here we show that patterns of introgression in a contemporary hybrid zone in Lycaeides butterflies predict patterns of ancestry in geographically adjacent, older hybrid populations. We find a particularly striking lack of ancestry from one of the hybridizing taxa, Lycaeides melissa, on the Z chromosome in both the old and contemporary hybrids. The same pattern of reduced L. melissa ancestry on the Z chromosome is seen in two …
Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey
Master's Theses
Aedes aegypti, the primary mosquito vector of the yellow fever virus, threatens global health by passing on this virus, as well as chikungunya, dengue, and Zika viruses. Through its natural tendency to live in highly urban areas and bite human hosts; understanding the factors that affect the historical and current range of the pest is invaluable (Gubler, 1998). Although these viruses are not normally found in the United States, lack of vaccinations and wide-spread presence of the mosquito could lead to these diseases being reintroduced with potentially devastating effects (Monath, 2001). To determine a partial historical range of A. aegypti …
An Overview Of Hypotheses And Supporting Evidence Regarding Drivers Of Insect Gigantism In The Permo-Carboniferous, Ryssa Parks
An Overview Of Hypotheses And Supporting Evidence Regarding Drivers Of Insect Gigantism In The Permo-Carboniferous, Ryssa Parks
WWU Honors College Senior Projects
Insects provide powerful examples of the responses of organisms to environmental change. For example, insect body size gives us insight into the consequences of climate change due to shifts in atmospheric composition, both in the present and in the past. Indeed, one common hypothesis behind the enormous sizes of insects during the late Carboniferous to early Permian (323.2 to 265.0 million years ago) is that such sizes were enabled by elevated oxygen levels (hyperoxia) during the Permo-Carboniferous, when atmospheric oxygen was as high as 60% greater than its present-day concentration. To examine whether the giant body sizes of insects were …
Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke
Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke
2020 Symposium Posters
The gut microbiome plays an essential role in the overall health of organisms. However, the presence and abundance of these microbes may be altered by environmental factors, such as exposure to pesticides. The goals of this project were to understand 1) the prevalence of pesticide residues in honey bees in eastern Washington, and 2) the impact of pesticides to the honey bee gut microbiome. Accordingly, we tested bees from 24 hives among six sites across eastern Washington. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was used to detect the presence and concentration of six commonly used agricultural pesticides: Carbaryl, Chlorpyrifos, Coumaphos, Cypermethrin, …
Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger
Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger
Master's Theses
Ground beetles (Carabidae) are increasingly used as ecological indicators in studies regarding land use because they are ubiquitous, respond quickly to environmental change, have a well-understood taxonomy, and can be trapped with ease. While the effects of various plant communities on ground beetle assemblages are relatively well-known, past studies have operated within boreal and tropical forests and have not placed much emphasis on the effects of native and nonnative species. In this study, ground beetles were investigated as indicators of invasion in a grassland setting. Ground beetles were sampled using pitfall traps throughout the 2018 growing season at Quivira National …
Fate Of White Fringetree Through The Invasion Wave Of Emerald Ash Borer And Its Variation In Resistance To Attack, Emily A. Ellison
Fate Of White Fringetree Through The Invasion Wave Of Emerald Ash Borer And Its Variation In Resistance To Attack, Emily A. Ellison
Browse all Theses and Dissertations
Ornamental white fringetrees in IL, IN, OH, and PA were reassessed in 2018 as a follow-up study to determine the fate of the white fringetree through the invasion wave of emerald ash borer. Attack rates decline by half from 2015-2018 and only 13% of trees were infested. Health of not reinfested trees in 2018 improved suggesting resilience against EAB attack whereas currently infested trees in 2018 displayed signs of declining health. Trees differed in their resistance to EAB attack and 41 additional ornamental and wild white fringetrees were studied in OH to determine if the anti-herbivory defense chemical, oleuropein, influenced …
Effects Of Forest Age And Composition On Coleoptera Associated With Fungal Fruiting Bodies In Southwest Ohio, Jeffrey M. Brown
Effects Of Forest Age And Composition On Coleoptera Associated With Fungal Fruiting Bodies In Southwest Ohio, Jeffrey M. Brown
Browse all Theses and Dissertations
Mature forests often harbor greater biodiversity than younger woods. As this relationship of forest age to biodiversity has not been examined for all taxa, this study sought to document the diversity of mycophilous beetle communities in deciduous forests of southwest Ohio and understand how they vary in relation to forest age. I surveyed fungus associated beetles using baited traps at eight forested sites in the Dayton, Ohio region. Traps were surveyed three times during 2018 to account for seasonal variation, something that has not been done for this geographic region. Forest age had no significant effect on beetle abundance or …
Establishment Of Wildflower Islands To Enhance Roadside Health And Aesthetics, Walter Schacht, Judy Wu-Smart
Establishment Of Wildflower Islands To Enhance Roadside Health And Aesthetics, Walter Schacht, Judy Wu-Smart
Nebraska Department of Transportation: Research Reports
Wildflowers are crucial in the ecological function of the low-input roadside plant communities in terms of water andnutrient cycling, nutrient inputs such as nitrogen, total plant canopy cover, stand longevity, and provision of habitat for numerous small animals. Further, wildflowers provide critical foraging and nesting resources for birds, insects, and other wildlife. Unfortunately, habitat loss from agricultural and urban development has led to rapid population declines in wild bees and other pollinators across the US, thereby jeopardizing not only food production but also the sustainability of our natural landscapes (Kearns & Inouye, 1997). One way to mitigate wild bee decline …
Mosquito Diversity And Positive Pools For The West Nile Virus In Forested Versus Non-Forested Areas, Elizabeth C. Paul
Mosquito Diversity And Positive Pools For The West Nile Virus In Forested Versus Non-Forested Areas, Elizabeth C. Paul
Honors Projects
The West Nile virus (WNV) is one of the leading causes of mosquito borne illness in the United States (CDC, 2018). This virus is a neurotropic pathogen that can cause fever and encephalitis. It is known that the Culex species is the primary vector for WNV. Humans infected with the WNV are typically asymptomatic. There is a small portion of individuals that experience West Nile fever which could include symptoms like high fever, vision loss, and coma (CDC, 2018). Previous studies have shown that mosquitoes tend to favor forests and wetlands (Hay et al., 1998), however, it is unknown if …
Effectiveness Of Different Agricultural Management Styles As Insect Biological Corridors: A Comparison Of Insect Populations In Fragmented Chocó Cloud Forest, Ecuador, Tara M. Krantz
Independent Study Project (ISP) Collection
Insects are part of the most diverse class of animals on the planet and are essential to various ecological functions such as pollination, nutrient cycling, providing a food source for other taxa, and more. The diversity and ecological services of insects are necessary to the operation of agriculture because of pest control and pollination of crops. However, the diversity of insects is severely reduced due to fragmentation. It is currently not well understood if certain types of agriculture can lessen the impact of fragmentation on natural and crop-based insect communities. In this study, insect populations in four different agricultural management …
Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli
Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli
Entomology Faculty Publications
Mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. Vector control is one of the most important approaches used in reducing the incidence of these diseases. However, increasing mosquito resistance to chemical insecticides presents challenges to this approach. Therefore, new strategies are necessary to develop the next generation vector control methods. Because of the target specificity of dsRNA, RNAi-based control measures are an attractive alternative to current insecticides used to control disease vectors. In this study, Chitosan (CS) was cross-linked to sodium tripolyphosphate (TPP) to produce nano-sized polyelectrolyte complexes with …
Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming
Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming
School of Natural Resources: Dissertations, Theses, and Student Research
Forensic entomology although not a commonly used discipline in the forensic sciences, does have its niche and when used by investigators is respected in crinimolegal investigations (Greenberg and Kunich, 2005). With many species of forensically significant insects being regionally specific, it is often difficult for forensic entomologists to as confidently translate regionally specific studies across drastically differing geographic regions (Brundage, et al., 2011).
The purpose of this study is to help create a better temporal and geographic distributional understanding of the blow fly species present in Los Angeles County, California, United States. Twenty-five locations from four ecoregions (coastal mountains, urban, …
Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss
Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss
School of Natural Resources: Dissertations, Theses, and Student Research
Forensic entomology is the application of the study of arthropods to the criminal justice system. This is primarily done through the development of a post mortem interval (PMI) based the insect evidence present. A practitioner must be able to determine the age of the insect through temperature data. One factor influencing the temperature dependent development is gregarious behavior. Current literature describes a faster development rate due to an increase in feeding efficiency and temperatures produced by this aggregate. However, there is very little literature defining a minimum number needed to induce this effect and little to none on it for …
The Effects Of European Fire Ants On Blacklegged Ticks In Acadia National Park, Lucy Guarnieri
The Effects Of European Fire Ants On Blacklegged Ticks In Acadia National Park, Lucy Guarnieri
Honors College
The blacklegged tick (Ixodes scapularis) and the European fire ant (Myrmica rubra) are notable invasive pests in Maine, especially across coastal and southern regions. I. scapularis is the primary vector of the bacterium Borrelia burgdorferi, which is the causative agent of Lyme disease, and Maine currently has one of the highest Lyme disease incidence rates in the U.S. Ticks have many natural predators, including ants. This study investigates the effects of M. rubra on I. scapularis abundance and pathogen infection prevalence in Acadia National Park (ANP). I collected ticks by drag-sampling at eight ant-infested sites and …
Geospatial Analysis Of Rickettsial Species In Arkansas, Amy D. Frank, Ashley P.G. Dowling
Geospatial Analysis Of Rickettsial Species In Arkansas, Amy D. Frank, Ashley P.G. Dowling
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Rickettsia species are obligate intracellular, arthropod-borne bacteria with a potential to cause multiple diseases including Rocky Mountain spotted fever (RMSF). Fleas, mites, and ticks serve as vectors for Rickettsia, but ticks are the primary vector of interest. RMSF and other rickettsial diseases have continued to gain importance in both human and veterinary medicine as RMSF is the most common tick-borne disease within the United States according to the Lyme and Tick-Borne Disease Research Center. A statewide citizen science project was utilized to determine the prevalence of Spotted Fever Group (SFG) Rickettsia in Arkansas. This project yielded results in 64 …
The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas
The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas
Graduate Theses, Dissertations, and Problem Reports
he use of entomological specimens as a source of forensic DNA evidence has been studied since the late 20th century. Currently as it stands, to preserve both the minimum postmortem interval (mPMI) and DNA evidence within fly larvae, two standards of preservation exist. For mPMI, the use of hot water kill with near-boiling water is used to fix larvae size for accurate age estimation. For DNA evidence, the standard technique of preservation is submersion in 70% ethanol. To help reduce the number of standard preservation techniques for practitioners, this study assessed the feasibility of the hot water kill preservation technique …
Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos
Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos
Graduate Theses, Dissertations, and Problem Reports
In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.
In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …
Impact Of Native Natural Enemies On Populations Of The Invasive Winter Moth (Operophtera Brumata L) In The Northeast United States, Hannah J. Broadley
Impact Of Native Natural Enemies On Populations Of The Invasive Winter Moth (Operophtera Brumata L) In The Northeast United States, Hannah J. Broadley
Doctoral Dissertations
Invasive insects increasingly affect forested landscapes and have important ecological and economic impacts. My dissertation focuses on population dynamics of winter moth (Operophtera brumata L.), an invasive pest in the northeastern United States. Native to Europe, this is the species’ fourth accidental introduction to North America. The Elkinton lab established the biological control agent Cyzenis albicans across the range of winter moth in the northeastern U.S. Prior research indicates that C. albicans’ ability to control winter moth likely depends on additional mortality from native natural enemies. My dissertation research evaluates the identity and role of natural enemies already …
Identification Of Target Genes For Rnai-Mediated Control Of The Twospotted Spider Mite, June-Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba Reddy Palli
Identification Of Target Genes For Rnai-Mediated Control Of The Twospotted Spider Mite, June-Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba Reddy Palli
Entomology Faculty Publications
RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids …
Clover Root Curculio (Sitona Hispidulus F.), Kaitlin Rim, Steven Price, Ricardo Ramirez
Clover Root Curculio (Sitona Hispidulus F.), Kaitlin Rim, Steven Price, Ricardo Ramirez
All Current Publications
The clover root curculio is an important agricultural pest in forage production systems. Adult beetles feed on the host plant leaves while the damaging larval stage feeds on the roots below ground. Heavy larval feeding has been associated with reduced stand establishment, disruption of nutrient and water uptake, increased secondary plant pathogen infection, decreased winter plant survival, delayed green-up, and reductions in forage quality and yield. Due to the hidden nature of eggs and larvae in the soil, CRC has been overlooked and damage is often misdiagnosed as nutrient deficiencies or pathogens. Historically, applications of chlorinated hydrocarbons, carbamates, and organophosphates …
Confirming World-Wide Distribution Of An Agriculturally Important Lacewing, Chrysoperla Zastrowi Sillemi, Using Songs, Morphology, Mitochondrial Gene Sequencing, And Phylogenetic Reconstruction, Zoe Mandese
Honors Scholar Theses
The Chrysoperla carnea-group of green lacewings is a cryptic species complex. Species within the group are morphologically similar, yet isolated from one another via reproductive mating song. Chrysoperla zastrowi, a species within the carnea-group, is currently described with a distribution ranging from South Africa to the Middle East and India. However, recent collections of carnea-group lacewings from Guatemala and California were preliminarily identified as Chrysoperla zastrowi based upon similarities in their vibrational courtship songs. This analysis aims to place six specimens, collected by collaborators in Guatemala, Armenia, Iran, and California, into a pre-existing phylogeny of the …
An Expanded Toolkit For Gene Tagging Based On Mimic And Scarless Crispr Tagging In, David Li-Kroeger, Oguz Kanca, Pei-Tseng Lee, Sierra Cowan, Michael T Lee, Manish Jaiswal, Jose Luis Salazar, Yuchun He, Zhongyuan Zuo, Hugo J Bellen
An Expanded Toolkit For Gene Tagging Based On Mimic And Scarless Crispr Tagging In, David Li-Kroeger, Oguz Kanca, Pei-Tseng Lee, Sierra Cowan, Michael T Lee, Manish Jaiswal, Jose Luis Salazar, Yuchun He, Zhongyuan Zuo, Hugo J Bellen
Faculty Publications
We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH significantly increases integration efficiency compared to previous strategies and faithfully reports the expression pattern of genes and proteins. The second technique targets genes lacking coding introns using a two-step cassette exchange. First, we replace the endogenous gene with an excisable compact dominant marker using CRISPR making a null allele. Second, the insertion is replaced …
Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams
Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams
Graduate Theses and Dissertations
Honey bee populations have been fluctuating within recent years. No one cause has been attributed to colony fluctuations due to the theory that multiple stressors interact with one another to impact colony health. Consequently, microorganisms such as internal parasites of honey bees have been understudied as a contributor to colony health decline.
Molecular diagnostics were utilized to detect the presence of two honey bee trypanosome parasites, Crithidia mellificae and Lotmaria passim, in managed and feral honey bee populations from eight states in the United States (USA). Because studies on trypanosome infections are lacking in the USA, it is important to …
Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger
Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger
Biology Faculty Publications
Bee viral ecology is a fascinating emerging area of research: viruses exert a range of effects on their hosts, exacerbate the impacts of other environmental stressors, and, importantly, are readily shared across multiple bee species in a community. However, our understanding of bee viral communities is limited, as it is primarily derived from studies of North American and European Apis mellifera populations. Here, we examined viruses in populations of A. mellifera and 11 other bee species from 9 countries, across 5 continents and Oceania. We developed a novel pipeline to rapidly, inexpensively, and robustly screen for bee viruses. This pipeline …
Identification Of Highly Effective Target Genes For Rnai-Mediated Control Of Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Jian J. Duan, Subba Reddy Palli, Lynne K. Rieske
Identification Of Highly Effective Target Genes For Rnai-Mediated Control Of Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Jian J. Duan, Subba Reddy Palli, Lynne K. Rieske
Entomology Faculty Publications
Recent study has shown that RNA interference (RNAi) is efficient in emerald ash borer (EAB), Agrilus planipennis, and that ingestion of double-stranded RNA (dsRNA) targeting specific genes causes gene silencing and mortality in neonates. Here, we report on the identification of highly effective target genes for RNAi-mediated control of EAB. We screened 13 candidate genes in neonate larvae and selected the most effective target genes for further investigation, including their effect on EAB adults and on a non-target organism, Tribolium castaneum. The two most efficient target genes selected, hsp (heat shock 70-kDa protein cognate 3) and shi (shibire), …