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Full-Text Articles in Life Sciences

Recent Hybrids Recapitulate Ancient Hybrid Outcomes, Samridhi Chaturvedi, Lauren K. Lucas, C. Alex Buerkle, James A. Fordyce, Matthew L. Forister, Chris C. Nice, Zachariah Gompert May 2020

Recent Hybrids Recapitulate Ancient Hybrid Outcomes, Samridhi Chaturvedi, Lauren K. Lucas, C. Alex Buerkle, James A. Fordyce, Matthew L. Forister, Chris C. Nice, Zachariah Gompert

Ecology Center Publications

Genomic outcomes of hybridization depend on selection and recombination in hybrids. Whether these processes have similar effects on hybrid genome composition in contemporary hybrid zones versus ancient hybrid lineages is unknown. Here we show that patterns of introgression in a contemporary hybrid zone in Lycaeides butterflies predict patterns of ancestry in geographically adjacent, older hybrid populations. We find a particularly striking lack of ancestry from one of the hybridizing taxa, Lycaeides melissa, on the Z chromosome in both the old and contemporary hybrids. The same pattern of reduced L. melissa ancestry on the Z chromosome is seen in two other ...


Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey Apr 2020

Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey

Master's Theses

Aedes aegypti, the primary mosquito vector of the yellow fever virus, threatens global health by passing on this virus, as well as chikungunya, dengue, and Zika viruses. Through its natural tendency to live in highly urban areas and bite human hosts; understanding the factors that affect the historical and current range of the pest is invaluable (Gubler, 1998). Although these viruses are not normally found in the United States, lack of vaccinations and wide-spread presence of the mosquito could lead to these diseases being reintroduced with potentially devastating effects (Monath, 2001). To determine a partial historical range of A. aegypti ...


Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger Jan 2020

Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger

Master's Theses

Ground beetles (Carabidae) are increasingly used as ecological indicators in studies regarding land use because they are ubiquitous, respond quickly to environmental change, have a well-understood taxonomy, and can be trapped with ease. While the effects of various plant communities on ground beetle assemblages are relatively well-known, past studies have operated within boreal and tropical forests and have not placed much emphasis on the effects of native and nonnative species. In this study, ground beetles were investigated as indicators of invasion in a grassland setting. Ground beetles were sampled using pitfall traps throughout the 2018 growing season at Quivira National ...


Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke Jan 2020

Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke

2020 Symposium Posters

The gut microbiome plays an essential role in the overall health of organisms. However, the presence and abundance of these microbes may be altered by environmental factors, such as exposure to pesticides. The goals of this project were to understand 1) the prevalence of pesticide residues in honey bees in eastern Washington, and 2) the impact of pesticides to the honey bee gut microbiome. Accordingly, we tested bees from 24 hives among six sites across eastern Washington. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was used to detect the presence and concentration of six commonly used agricultural pesticides: Carbaryl, Chlorpyrifos, Coumaphos, Cypermethrin ...


Establishment Of Wildflower Islands To Enhance Roadside Health And Aesthetics, Walter Schacht, Judy Wu-Smart Dec 2019

Establishment Of Wildflower Islands To Enhance Roadside Health And Aesthetics, Walter Schacht, Judy Wu-Smart

Nebraska Department of Transportation Research Reports

Wildflowers are crucial in the ecological function of the low-input roadside plant communities in terms of water andnutrient cycling, nutrient inputs such as nitrogen, total plant canopy cover, stand longevity, and provision of habitat for numerous small animals. Further, wildflowers provide critical foraging and nesting resources for birds, insects, and other wildlife. Unfortunately, habitat loss from agricultural and urban development has led to rapid population declines in wild bees and other pollinators across the US, thereby jeopardizing not only food production but also the sustainability of our natural landscapes (Kearns & Inouye, 1997). One way to mitigate wild bee decline is ...


Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli Jun 2019

Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli

Entomology Faculty Publications

Mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. Vector control is one of the most important approaches used in reducing the incidence of these diseases. However, increasing mosquito resistance to chemical insecticides presents challenges to this approach. Therefore, new strategies are necessary to develop the next generation vector control methods. Because of the target specificity of dsRNA, RNAi-based control measures are an attractive alternative to current insecticides used to control disease vectors. In this study, Chitosan (CS) was cross-linked to sodium tripolyphosphate (TPP) to produce nano-sized polyelectrolyte complexes with ...


Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming Apr 2019

Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming

Dissertations & Theses in Natural Resources

Forensic entomology although not a commonly used discipline in the forensic sciences, does have its niche and when used by investigators is respected in crinimolegal investigations (Greenberg and Kunich, 2005). With many species of forensically significant insects being regionally specific, it is often difficult for forensic entomologists to as confidently translate regionally specific studies across drastically differing geographic regions (Brundage, et al., 2011).

The purpose of this study is to help create a better temporal and geographic distributional understanding of the blow fly species present in Los Angeles County, California, United States. Twenty-five locations from four ecoregions (coastal mountains, urban ...


Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss Apr 2019

Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss

Dissertations & Theses in Natural Resources

Forensic entomology is the application of the study of arthropods to the criminal justice system. This is primarily done through the development of a post mortem interval (PMI) based the insect evidence present. A practitioner must be able to determine the age of the insect through temperature data. One factor influencing the temperature dependent development is gregarious behavior. Current literature describes a faster development rate due to an increase in feeding efficiency and temperatures produced by this aggregate. However, there is very little literature defining a minimum number needed to induce this effect and little to none on it for ...


The Effects Of European Fire Ants On Blacklegged Ticks In Acadia National Park, Lucy Guarnieri Apr 2019

The Effects Of European Fire Ants On Blacklegged Ticks In Acadia National Park, Lucy Guarnieri

Honors College

The blacklegged tick (Ixodes scapularis) and the European fire ant (Myrmica rubra) are notable invasive pests in Maine, especially across coastal and southern regions. I. scapularis is the primary vector of the bacterium Borrelia burgdorferi, which is the causative agent of Lyme disease, and Maine currently has one of the highest Lyme disease incidence rates in the U.S. Ticks have many natural predators, including ants. This study investigates the effects of M. rubra on I. scapularis abundance and pathogen infection prevalence in Acadia National Park (ANP). I collected ticks by drag-sampling at eight ant-infested sites and eight control sites ...


Geospatial Analysis Of Rickettsial Species In Arkansas, Amy D. Frank, Ashley P.G. Dowling Jan 2019

Geospatial Analysis Of Rickettsial Species In Arkansas, Amy D. Frank, Ashley P.G. Dowling

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Rickettsia species are obligate intracellular, arthropod-borne bacteria with a potential to cause multiple diseases including Rocky Mountain spotted fever (RMSF). Fleas, mites, and ticks serve as vectors for Rickettsia, but ticks are the primary vector of interest. RMSF and other rickettsial diseases have continued to gain importance in both human and veterinary medicine as RMSF is the most common tick-borne disease within the United States according to the Lyme and Tick-Borne Disease Research Center. A statewide citizen science project was utilized to determine the prevalence of Spotted Fever Group (SFG) Rickettsia in Arkansas. This project yielded results in 64 of ...


The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas Jan 2019

The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas

Graduate Theses, Dissertations, and Problem Reports

he use of entomological specimens as a source of forensic DNA evidence has been studied since the late 20th century. Currently as it stands, to preserve both the minimum postmortem interval (mPMI) and DNA evidence within fly larvae, two standards of preservation exist. For mPMI, the use of hot water kill with near-boiling water is used to fix larvae size for accurate age estimation. For DNA evidence, the standard technique of preservation is submersion in 70% ethanol. To help reduce the number of standard preservation techniques for practitioners, this study assessed the feasibility of the hot water kill preservation technique ...


Impact Of Native Natural Enemies On Populations Of The Invasive Winter Moth (Operophtera Brumata L) In The Northeast United States, Hannah J. Broadley Oct 2018

Impact Of Native Natural Enemies On Populations Of The Invasive Winter Moth (Operophtera Brumata L) In The Northeast United States, Hannah J. Broadley

Doctoral Dissertations

Invasive insects increasingly affect forested landscapes and have important ecological and economic impacts. My dissertation focuses on population dynamics of winter moth (Operophtera brumata L.), an invasive pest in the northeastern United States. Native to Europe, this is the species’ fourth accidental introduction to North America. The Elkinton lab established the biological control agent Cyzenis albicans across the range of winter moth in the northeastern U.S. Prior research indicates that C. albicans’ ability to control winter moth likely depends on additional mortality from native natural enemies. My dissertation research evaluates the identity and role of natural enemies already present ...


Identification Of Target Genes For Rnai-Mediated Control Of The Twospotted Spider Mite, June-Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba Reddy Palli Oct 2018

Identification Of Target Genes For Rnai-Mediated Control Of The Twospotted Spider Mite, June-Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba Reddy Palli

Entomology Faculty Publications

RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids ...


Clover Root Curculio (Sitona Hispidulus F.), Kaitlin Rim, Steven Price, Ricardo Ramirez Oct 2018

Clover Root Curculio (Sitona Hispidulus F.), Kaitlin Rim, Steven Price, Ricardo Ramirez

All Current Publications

The clover root curculio is an important agricultural pest in forage production systems. Adult beetles feed on the host plant leaves while the damaging larval stage feeds on the roots below ground. Heavy larval feeding has been associated with reduced stand establishment, disruption of nutrient and water uptake, increased secondary plant pathogen infection, decreased winter plant survival, delayed green-up, and reductions in forage quality and yield. Due to the hidden nature of eggs and larvae in the soil, CRC has been overlooked and damage is often misdiagnosed as nutrient deficiencies or pathogens. Historically, applications of chlorinated hydrocarbons, carbamates, and organophosphates ...


Confirming World-Wide Distribution Of An Agriculturally Important Lacewing, Chrysoperla Zastrowi Sillemi, Using Songs, Morphology, Mitochondrial Gene Sequencing, And Phylogenetic Reconstruction, Zoe Mandese Aug 2018

Confirming World-Wide Distribution Of An Agriculturally Important Lacewing, Chrysoperla Zastrowi Sillemi, Using Songs, Morphology, Mitochondrial Gene Sequencing, And Phylogenetic Reconstruction, Zoe Mandese

Honors Scholar Theses

The Chrysoperla carnea-group of green lacewings is a cryptic species complex. Species within the group are morphologically similar, yet isolated from one another via reproductive mating song. Chrysoperla zastrowi, a species within the carnea-group, is currently described with a distribution ranging from South Africa to the Middle East and India. However, recent collections of carnea-group lacewings from Guatemala and California were preliminarily identified as Chrysoperla zastrowi based upon similarities in their vibrational courtship songs. This analysis aims to place six specimens, collected by collaborators in Guatemala, Armenia, Iran, and California, into a pre-existing phylogeny of the Chrysoperla ...


Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams Aug 2018

Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams

Theses and Dissertations

Honey bee populations have been fluctuating within recent years. No one cause has been attributed to colony fluctuations due to the theory that multiple stressors interact with one another to impact colony health. Consequently, microorganisms such as internal parasites of honey bees have been understudied as a contributor to colony health decline.

Molecular diagnostics were utilized to detect the presence of two honey bee trypanosome parasites, Crithidia mellificae and Lotmaria passim, in managed and feral honey bee populations from eight states in the United States (USA). Because studies on trypanosome infections are lacking in the USA, it is important to ...


Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger Jun 2018

Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger

Biology Faculty Publications

Bee viral ecology is a fascinating emerging area of research: viruses exert a range of effects on their hosts, exacerbate the impacts of other environmental stressors, and, importantly, are readily shared across multiple bee species in a community. However, our understanding of bee viral communities is limited, as it is primarily derived from studies of North American and European Apis mellifera populations. Here, we examined viruses in populations of A. mellifera and 11 other bee species from 9 countries, across 5 continents and Oceania. We developed a novel pipeline to rapidly, inexpensively, and robustly screen for bee viruses. This pipeline ...


Identification Of Highly Effective Target Genes For Rnai-Mediated Control Of Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Jian J Duan, Subba Reddy Palli, Lynne K. Rieske Mar 2018

Identification Of Highly Effective Target Genes For Rnai-Mediated Control Of Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Jian J Duan, Subba Reddy Palli, Lynne K. Rieske

Entomology Faculty Publications

Recent study has shown that RNA interference (RNAi) is efficient in emerald ash borer (EAB), Agrilus planipennis, and that ingestion of double-stranded RNA (dsRNA) targeting specific genes causes gene silencing and mortality in neonates. Here, we report on the identification of highly effective target genes for RNAi-mediated control of EAB. We screened 13 candidate genes in neonate larvae and selected the most effective target genes for further investigation, including their effect on EAB adults and on a non-target organism, Tribolium castaneum. The two most efficient target genes selected, hsp (heat shock 70-kDa protein cognate 3) and shi (shibire), caused up ...


Creb-Binding Protein Plays Key Roles In Juvenile Hormone Action In The Red Flour Beetle, Tribolium Castaneum, Jingjing Xu, Amit Roy, Subba Reddy Palli Jan 2018

Creb-Binding Protein Plays Key Roles In Juvenile Hormone Action In The Red Flour Beetle, Tribolium Castaneum, Jingjing Xu, Amit Roy, Subba Reddy Palli

Entomology Faculty Publications

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and ...


Comparing Created And Natural Depressional Wetlands Through Trophic Analysis Of Macroinvertebrates, Shante N. Eisele Jan 2018

Comparing Created And Natural Depressional Wetlands Through Trophic Analysis Of Macroinvertebrates, Shante N. Eisele

Browse all Theses and Dissertations

Macroinvertebrates are important contributors to wetland ecosystems due to their role in decomposition, nutrient cycling, and as a food resource for other organisms. Several studies have analyzed the macroinvertebrate communities in created wetlands, but few have evaluated them in the context of trophic structure in both created and natural wetlands. The objective of this study is to better understand benthic macroinvertebrate community composition and trophic structure in created and natural wetlands. My central hypotheses were that macroinvertebrate communities in created wetlands would have (1) differing composition and (2) less complex trophic structure with shorter food-chain length compared to natural wetlands ...


Unknown And Cryptic Diversity In The Adelpha Serpa-Group, Cassidi Rush Jan 2018

Unknown And Cryptic Diversity In The Adelpha Serpa-Group, Cassidi Rush

University of the Pacific Theses and Dissertations

The tropics are host to incredible insect diversity. One of the most charismatic groups that exhibit this diversity are the butterflies. Despite the tropical butterfly fauna being heavily researched, there remains much hidden diversity in the form of undescribed life histories and cryptic species. This is especially true among the species rich Nymphalidae, the brush-footed butterflies. Species in the genus Adelpha are known to be “the most trying taxonomically of all nymphalids” DeVries (1987), and as such are fruitful ground for uncovering unknown diversity. About half of the species within Adelpha have undescribed life histories, while A. serpa stands out ...


Hart Prairie Preserve Site Visit To Assess Aspen Health Nature Conservancy, Flagstaff, Az, Amanda Grady Jan 2018

Hart Prairie Preserve Site Visit To Assess Aspen Health Nature Conservancy, Flagstaff, Az, Amanda Grady

Aspen Bibliography

On June 11, 2018, Amanda Grady, Entomologist, Arizona Zone, Forest Health Protection, performed a site visit to evaluate aspen health on the Nature Conservancy lands at Heart Prairie Preserve (HPP) near Flagstaff. Specifically, the evaluation was to determine whether or not the aspen within exclosures on Fern Mountain have symptoms or signs of Oystershell scale (OSS), an emergent forest pest contributing to aspen decline in northern Arizona.


A Comparison Of Two Methods Of Quantifying Mating Success In Low Density Gypsy Moth (Lymantria Dispar) Populations, Alexandra Barry, Hannah Byrne, Derek M. Johnson Jan 2018

A Comparison Of Two Methods Of Quantifying Mating Success In Low Density Gypsy Moth (Lymantria Dispar) Populations, Alexandra Barry, Hannah Byrne, Derek M. Johnson

Undergraduate Research Posters

The gypsy moth (Lymantria dispar) is a defoliating pest native to Europe and invasive to North America. The gypsy moth is subject to depressed mating success in low density populations, which may restrict spread of the forest pest. Research focusing on gypsy moth density as it relates to mating behavior has often used counts of males caught in pheromone-baited delta traps as a proxy to estimate the probability of female mating success. The purpose of this project was to determine whether pheromone trap counts provide accurate estimates of female mating success probability, by comparing data gathered from pheromone-baited delta traps ...


Comparative Analysis Of Double-Stranded Rna Degradation And Processing In Insects, Indrakant K. Singh, Satnam Singh, Kanakachari Mogilicherla, Jayendra Nath Shukla, Subba R. Palli Dec 2017

Comparative Analysis Of Double-Stranded Rna Degradation And Processing In Insects, Indrakant K. Singh, Satnam Singh, Kanakachari Mogilicherla, Jayendra Nath Shukla, Subba R. Palli

Entomology Faculty Publications

RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within ...


A Molecular Diagnostic Survey Of Pathogens And Parasites Of Honey Bees, Apis Mellifera L., From Arkansas And Oklahoma, Dylan Cleary Dec 2017

A Molecular Diagnostic Survey Of Pathogens And Parasites Of Honey Bees, Apis Mellifera L., From Arkansas And Oklahoma, Dylan Cleary

Theses and Dissertations

The health and viability of colonies of the honey bee, Apis mellifera, in the United States have fluctuated dramatically over the past decade. This poses a substantial threat to agricultural production in this country. Currently, no single factor has been identified for this decline. Rather, it has been suggested that the interaction between multiple biotic and abiotic stressors may be responsible. Among these factors are pesticides, habitat loss, climate and weather, parasites and pathogens, and colony management techniques. For this reason, it is important to examine the prevalence of honey bee parasite and pathogen infection at the state level in ...


Temporal Transcriptomic Profiling Of The Ant-Feeding Assassin Bug Acanthaspis Cincticrus Reveals A Biased Expression Of Genes Associated With Predation In Nymphs, Fei Kou, Hu Li, Shujuan Li, Huaizhu Xun, Yinqiao Zhang, Ziqiang Sun, Xuguo Zhou, Wanzhi Cai Oct 2017

Temporal Transcriptomic Profiling Of The Ant-Feeding Assassin Bug Acanthaspis Cincticrus Reveals A Biased Expression Of Genes Associated With Predation In Nymphs, Fei Kou, Hu Li, Shujuan Li, Huaizhu Xun, Yinqiao Zhang, Ziqiang Sun, Xuguo Zhou, Wanzhi Cai

Entomology Faculty Publications

Acanthaspis cincticrus (Stål) is an assassin bug with a specialized camouflaging behavior to ambush ants in the nymphal stages. In this study, we comprehensively sequenced all the life stages of A. cincticrus, including the eggs, five nymph instars, female and male adults using Illumina HiSeq technology. We obtained 176 million clean sequence reads. The assembled 84,055 unigenes were annotated and classified functionally based on protein databases. Among the unigenes, 29.03% were annotated by one or more databases, suggesting their well-conserved functions. Comparison of the gene expression profiles in the egg, nymph and adult stages revealed certain bias. Functional ...


Rna Interference In The Asian Longhorned Beetle: Identification Of Key Rnai Genes And Reference Genes For Rt-Qpcr, Thais B. Rodrigues, Ramesh Kumar Dhandapani, Jian J. Duan, Subba Reddy Palli Aug 2017

Rna Interference In The Asian Longhorned Beetle: Identification Of Key Rnai Genes And Reference Genes For Rt-Qpcr, Thais B. Rodrigues, Ramesh Kumar Dhandapani, Jian J. Duan, Subba Reddy Palli

Entomology Faculty Publications

Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding proteins (dsRBP) as well as for proteins involved in dsRNA transport and the systemic RNAi. We also compared expression of six potential reference genes that could be used to normalize gene expression and selected gapdh and rpl32 as the most reliable genes among different tissues and stages ...


Development Of Rnai Method For Screening Candidate Genes To Control Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Lynne K. Rieske, Jian J. Duan, Kanakachari Mogilicherla, Subba Reddy Palli Aug 2017

Development Of Rnai Method For Screening Candidate Genes To Control Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Lynne K. Rieske, Jian J. Duan, Kanakachari Mogilicherla, Subba Reddy Palli

Entomology Faculty Publications

The ingestion of double-strand RNAs (dsRNA) targeting essential genes in an insect could cause mortality. Based on this principle, a new generation of insect control methods using RNA interference (RNAi) are being developed. In this work, we developed a bioassay for oral delivery of dsRNA to an invasive forest and urban tree pest, the emerald ash borer (EAB, Agrilus planipennis). EAB feeds and develops beneath the bark, killing trees rapidly. This behavior, coupled with the lack of a reliable artificial diet for rearing larvae and adults, make them difficult to study. We found that dsRNA is transported and processed to ...


Brown Marmorated Stink Bug [Halyomorpha Halys (Stal)], Mark Cody Holthouse, Diane G. Alston, Lori R. Spears, Erin Petrizzo Jul 2017

Brown Marmorated Stink Bug [Halyomorpha Halys (Stal)], Mark Cody Holthouse, Diane G. Alston, Lori R. Spears, Erin Petrizzo

All Current Publications

This fact sheet describes the brown marmorated stink bug, an invasive insect pest from eastern Asia. It includes a description of where it is found in Utah, host plants, crop injury and plant damage, general description of life stages, agricultural risks, monitoring, management, insecticides, and what to do if you find it.


"Fanciful But Not Forgotten: A Historical Examination Of The Study Of The Flea, 1840-1930", Andrea Jean Buhler May 2017

"Fanciful But Not Forgotten: A Historical Examination Of The Study Of The Flea, 1840-1930", Andrea Jean Buhler

Theses and Dissertations

Although the Victorian and Progressive periods saw a rise in entomological research, fleas were not a priority for scientific investigation. The discovery of fleas as disease vectors in the late nineteenth century marked a turning-point in interest in fleas. Expanding this standard history of fleas, the thesis probes flea research conducted outside the confines of disease during 1840-1930. It documents and analyzes the contributions of Louis Bertolotto, William Heckler, Charles Rothschild, Karl Jordan, and L. O. Howard. Whereas those working in the new profession of entomology saw fleas as disease vectors, these men had different relationships with fleas: Bertolotto and ...