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Full-Text Articles in Life Sciences

Intragenic Dna Methylation Regulates Insect Gene Expression And Reproduction Through The Mbd/Tip60 Complex, Guanfeng Xu, Hao Lyu, Yangqin Yi, Yuling Peng, Qili Feng, Qisheng Song, Chengcheng Gong, Xuezhen Peng, Subba Reddy Palli, Sichun Zheng Jan 2021

Intragenic Dna Methylation Regulates Insect Gene Expression And Reproduction Through The Mbd/Tip60 Complex, Guanfeng Xu, Hao Lyu, Yangqin Yi, Yuling Peng, Qili Feng, Qisheng Song, Chengcheng Gong, Xuezhen Peng, Subba Reddy Palli, Sichun Zheng

Entomology Faculty Publications

DNA methylation is an important epigenetic modification. However, the regulations and functions of insect intragenic DNA methylation remain unknown. Here, we demonstrate that a regulatory mechanism involving intragenic DNA methylation controls ovarian and embryonic developmental processes in Bombyx mori. In B. mori, DNA methylation is found near the transcription start site (TSS) of ovarian genes. By promoter activity analysis, we observed that 5′ UTR methylation enhances gene expression. Moreover, methyl-DNA-binding domain protein 2/3 (MBD2/3) binds to the intragenic methyl-CpG fragment and recruits acetyltransferase Tip60 to promote histone H3K27 acetylation and gene expression. Additionally, genome-wide analyses showed that the ...


Scoping Review Of Distribution Models For Selected Amblyomma Ticks And Rickettsial Group Pathogens, Catherine A. Lippi, Holly D. Gaff, Alexis L. White, Sadie J. Ryan Jan 2021

Scoping Review Of Distribution Models For Selected Amblyomma Ticks And Rickettsial Group Pathogens, Catherine A. Lippi, Holly D. Gaff, Alexis L. White, Sadie J. Ryan

Biological Sciences Faculty Publications

The rising prevalence of tick-borne diseases in humans in recent decades has called attention to the need for more information on geographic risk for public health planning. Species distribution models (SDMs) are an increasingly utilized method of constructing potential geographic ranges. There are many knowledge gaps in our understanding of risk of exposure to tick-borne pathogens, particularly for those in the rickettsial group. Here, we conducted a systematic scoping review of the SDM literature for rickettsial pathogens and tick vectors in the genus Amblyomma. Of the 174 reviewed articles, only 24 studies used SDMs to estimate the potential extent of ...


Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens [Research Data], Daniel A. Potter Oct 2020

Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens [Research Data], Daniel A. Potter

Entomology Research Data

Public interest in ecological landscaping and gardening is fueling a robust market for native plants. Most plants available to consumers through the horticulture trade are cultivated forms that have been selected for modified flowers or foliage, compactness, or other ornamental characteristics. Depending on their traits, some native plant cultivars seem to support pollinators, specialist insect folivores, and insect-based vertebrate food webs as effectively as native plant species, whereas others do not. There is particular need for information on whether native cultivars can be as effective as true or “wild-type” native species for supporting specialist native insects of conservation concern. Herein ...


Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens, Adam M. Baker, Carl T. Redmond, Stephen B. Malcolm, Daniel A. Potter Sep 2020

Suitability Of Native Milkweed (Asclepias) Species Versus Cultivars For Supporting Monarch Butterflies And Bees In Urban Gardens, Adam M. Baker, Carl T. Redmond, Stephen B. Malcolm, Daniel A. Potter

Entomology Faculty Publications

Public interest in ecological landscaping and gardening is fueling a robust market for native plants. Most plants available to consumers through the horticulture trade are cultivated forms that have been selected for modified flowers or foliage, compactness, or other ornamental characteristics. Depending on their traits, some native plant cultivars seem to support pollinators, specialist insect folivores, and insect-based vertebrate food webs as effectively as native plant species, whereas others do not. There is particular need for information on whether native cultivars can be as effective as true or “wild-type” native species for supporting specialist native insects of conservation concern. Herein ...


Data For Rosenberger & Conforti. Native And Agricultural Grassland Use By Stable And Declining Bumble Bees In Midwestern North America. Insect Cons. & Div., Derek W. Rosenberger, Mckenna Conforti Sep 2020

Data For Rosenberger & Conforti. Native And Agricultural Grassland Use By Stable And Declining Bumble Bees In Midwestern North America. Insect Cons. & Div., Derek W. Rosenberger, Mckenna Conforti

Faculty Scholarship – Biology

Little is known about how agricultural and native grasslands impact bumble bee populations in the Midwestern United States. We surveyed bumble bee populations over 3 years (2017-2019) at Midewin National Tallgrass Prairie in restored prairie, cattle and bison pastures. Raw data from that work is included in this file. These data were used in the manuscript "Native and agricultural grassland use by stable and declining bumble bees in Midwestern North America" by Derek Rosenberger and McKenna Conforti, and published in the journal Insect Conservation and Diversity in 2020.


Wireworms, Ryan Davis, Jay B. Karren, Alan H. Roe Aug 2020

Wireworms, Ryan Davis, Jay B. Karren, Alan H. Roe

All Current Publications

Wireworms are the larvae (immatures) of click beetles. Adults are elongate, slender, hardshelled beetles that take their name from their habit of flipping into the air (sometimes with an audible clicking sound) when they are placed on their backs. This fact sheet reviews the management of wireworms.


The Impacts Of Prescribed Burning And Mechanical Thinning On Insect Communities In The Arkansas Ozarks, Aaron P. Tormanen Aug 2020

The Impacts Of Prescribed Burning And Mechanical Thinning On Insect Communities In The Arkansas Ozarks, Aaron P. Tormanen

Theses and Dissertations from 2020

Insects are important in woodland ecosystems due to their role as pollinators and as prey for bats. My research investigated the relationships between forest management, vegetation, and insects in the Ozark National Forest in Arkansas. I selected 30 stands burned at varying frequencies in the last 12 years. Twelve of these stands were burned and mechanically thinned, 12 were only burned, and 6 were untreated. I deployed blacklight traps and malaise traps in each stand monthly from mid-March to mid- November 2019. Over 42,391 insects were collected, and Lepidoptera was the most abundant order. Insects were dried, weighed, and ...


The Role Of Body Farms And Forensic Entomology In Understanding The Decomposition Of Human Cadavers, Kaylee D'Eon May 2020

The Role Of Body Farms And Forensic Entomology In Understanding The Decomposition Of Human Cadavers, Kaylee D'Eon

Honors Theses

Forensic biology is an area which analyzes forms of evidence such as hair or blood and more. These tasks are so critical when it comes to looking at a crime scene and trying to figure out what happened during a case.

Another important aspect of forensic biology is the other work being done at body farms. Not many people know body farms exist especially if you live along the seacoast because a greater portion of the body farms are in the middle of the United States. The main point of body farms is to construct different scenes that can occur ...


Recent Hybrids Recapitulate Ancient Hybrid Outcomes, Samridhi Chaturvedi, Lauren K. Lucas, C. Alex Buerkle, James A. Fordyce, Matthew L. Forister, Chris C. Nice, Zachariah Gompert May 2020

Recent Hybrids Recapitulate Ancient Hybrid Outcomes, Samridhi Chaturvedi, Lauren K. Lucas, C. Alex Buerkle, James A. Fordyce, Matthew L. Forister, Chris C. Nice, Zachariah Gompert

Ecology Center Publications

Genomic outcomes of hybridization depend on selection and recombination in hybrids. Whether these processes have similar effects on hybrid genome composition in contemporary hybrid zones versus ancient hybrid lineages is unknown. Here we show that patterns of introgression in a contemporary hybrid zone in Lycaeides butterflies predict patterns of ancestry in geographically adjacent, older hybrid populations. We find a particularly striking lack of ancestry from one of the hybridizing taxa, Lycaeides melissa, on the Z chromosome in both the old and contemporary hybrids. The same pattern of reduced L. melissa ancestry on the Z chromosome is seen in two other ...


Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey Apr 2020

Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey

Master's Theses

Aedes aegypti, the primary mosquito vector of the yellow fever virus, threatens global health by passing on this virus, as well as chikungunya, dengue, and Zika viruses. Through its natural tendency to live in highly urban areas and bite human hosts; understanding the factors that affect the historical and current range of the pest is invaluable (Gubler, 1998). Although these viruses are not normally found in the United States, lack of vaccinations and wide-spread presence of the mosquito could lead to these diseases being reintroduced with potentially devastating effects (Monath, 2001). To determine a partial historical range of A. aegypti ...


An Overview Of Hypotheses And Supporting Evidence Regarding Drivers Of Insect Gigantism In The Permo-Carboniferous, Ryssa Parks Apr 2020

An Overview Of Hypotheses And Supporting Evidence Regarding Drivers Of Insect Gigantism In The Permo-Carboniferous, Ryssa Parks

WWU Honors Program Senior Projects

Insects provide powerful examples of the responses of organisms to environmental change. For example, insect body size gives us insight into the consequences of climate change due to shifts in atmospheric composition, both in the present and in the past. Indeed, one common hypothesis behind the enormous sizes of insects during the late Carboniferous to early Permian (323.2 to 265.0 million years ago) is that such sizes were enabled by elevated oxygen levels (hyperoxia) during the Permo-Carboniferous, when atmospheric oxygen was as high as 60% greater than its present-day concentration. To examine whether the giant body sizes of ...


Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke Jan 2020

Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke

2020 Symposium Posters

The gut microbiome plays an essential role in the overall health of organisms. However, the presence and abundance of these microbes may be altered by environmental factors, such as exposure to pesticides. The goals of this project were to understand 1) the prevalence of pesticide residues in honey bees in eastern Washington, and 2) the impact of pesticides to the honey bee gut microbiome. Accordingly, we tested bees from 24 hives among six sites across eastern Washington. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was used to detect the presence and concentration of six commonly used agricultural pesticides: Carbaryl, Chlorpyrifos, Coumaphos, Cypermethrin ...


Effects Of Forest Age And Composition On Coleoptera Associated With Fungal Fruiting Bodies In Southwest Ohio, Jeffrey M. Brown Jan 2020

Effects Of Forest Age And Composition On Coleoptera Associated With Fungal Fruiting Bodies In Southwest Ohio, Jeffrey M. Brown

Browse all Theses and Dissertations

Mature forests often harbor greater biodiversity than younger woods. As this relationship of forest age to biodiversity has not been examined for all taxa, this study sought to document the diversity of mycophilous beetle communities in deciduous forests of southwest Ohio and understand how they vary in relation to forest age. I surveyed fungus associated beetles using baited traps at eight forested sites in the Dayton, Ohio region. Traps were surveyed three times during 2018 to account for seasonal variation, something that has not been done for this geographic region. Forest age had no significant effect on beetle abundance or ...


Fate Of White Fringetree Through The Invasion Wave Of Emerald Ash Borer And Its Variation In Resistance To Attack, Emily A. Ellison Jan 2020

Fate Of White Fringetree Through The Invasion Wave Of Emerald Ash Borer And Its Variation In Resistance To Attack, Emily A. Ellison

Browse all Theses and Dissertations

Ornamental white fringetrees in IL, IN, OH, and PA were reassessed in 2018 as a follow-up study to determine the fate of the white fringetree through the invasion wave of emerald ash borer. Attack rates decline by half from 2015-2018 and only 13% of trees were infested. Health of not reinfested trees in 2018 improved suggesting resilience against EAB attack whereas currently infested trees in 2018 displayed signs of declining health. Trees differed in their resistance to EAB attack and 41 additional ornamental and wild white fringetrees were studied in OH to determine if the anti-herbivory defense chemical, oleuropein, influenced ...


Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger Jan 2020

Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger

Master's Theses

Ground beetles (Carabidae) are increasingly used as ecological indicators in studies regarding land use because they are ubiquitous, respond quickly to environmental change, have a well-understood taxonomy, and can be trapped with ease. While the effects of various plant communities on ground beetle assemblages are relatively well-known, past studies have operated within boreal and tropical forests and have not placed much emphasis on the effects of native and nonnative species. In this study, ground beetles were investigated as indicators of invasion in a grassland setting. Ground beetles were sampled using pitfall traps throughout the 2018 growing season at Quivira National ...


Establishment Of Wildflower Islands To Enhance Roadside Health And Aesthetics, Walter Schacht, Judy Wu-Smart Dec 2019

Establishment Of Wildflower Islands To Enhance Roadside Health And Aesthetics, Walter Schacht, Judy Wu-Smart

Nebraska Department of Transportation Research Reports

Wildflowers are crucial in the ecological function of the low-input roadside plant communities in terms of water andnutrient cycling, nutrient inputs such as nitrogen, total plant canopy cover, stand longevity, and provision of habitat for numerous small animals. Further, wildflowers provide critical foraging and nesting resources for birds, insects, and other wildlife. Unfortunately, habitat loss from agricultural and urban development has led to rapid population declines in wild bees and other pollinators across the US, thereby jeopardizing not only food production but also the sustainability of our natural landscapes (Kearns & Inouye, 1997). One way to mitigate wild bee decline is ...


Effectiveness Of Different Agricultural Management Styles As Insect Biological Corridors: A Comparison Of Insect Populations In Fragmented Chocó Cloud Forest, Ecuador, Tara M. Krantz Oct 2019

Effectiveness Of Different Agricultural Management Styles As Insect Biological Corridors: A Comparison Of Insect Populations In Fragmented Chocó Cloud Forest, Ecuador, Tara M. Krantz

Independent Study Project (ISP) Collection

Insects are part of the most diverse class of animals on the planet and are essential to various ecological functions such as pollination, nutrient cycling, providing a food source for other taxa, and more. The diversity and ecological services of insects are necessary to the operation of agriculture because of pest control and pollination of crops. However, the diversity of insects is severely reduced due to fragmentation. It is currently not well understood if certain types of agriculture can lessen the impact of fragmentation on natural and crop-based insect communities. In this study, insect populations in four different agricultural management ...


Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli Jun 2019

Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli

Entomology Faculty Publications

Mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. Vector control is one of the most important approaches used in reducing the incidence of these diseases. However, increasing mosquito resistance to chemical insecticides presents challenges to this approach. Therefore, new strategies are necessary to develop the next generation vector control methods. Because of the target specificity of dsRNA, RNAi-based control measures are an attractive alternative to current insecticides used to control disease vectors. In this study, Chitosan (CS) was cross-linked to sodium tripolyphosphate (TPP) to produce nano-sized polyelectrolyte complexes with ...


Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming Apr 2019

Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming

Dissertations & Theses in Natural Resources

Forensic entomology although not a commonly used discipline in the forensic sciences, does have its niche and when used by investigators is respected in crinimolegal investigations (Greenberg and Kunich, 2005). With many species of forensically significant insects being regionally specific, it is often difficult for forensic entomologists to as confidently translate regionally specific studies across drastically differing geographic regions (Brundage, et al., 2011).

The purpose of this study is to help create a better temporal and geographic distributional understanding of the blow fly species present in Los Angeles County, California, United States. Twenty-five locations from four ecoregions (coastal mountains, urban ...


Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss Apr 2019

Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss

Dissertations & Theses in Natural Resources

Forensic entomology is the application of the study of arthropods to the criminal justice system. This is primarily done through the development of a post mortem interval (PMI) based the insect evidence present. A practitioner must be able to determine the age of the insect through temperature data. One factor influencing the temperature dependent development is gregarious behavior. Current literature describes a faster development rate due to an increase in feeding efficiency and temperatures produced by this aggregate. However, there is very little literature defining a minimum number needed to induce this effect and little to none on it for ...


The Effects Of European Fire Ants On Blacklegged Ticks In Acadia National Park, Lucy Guarnieri Apr 2019

The Effects Of European Fire Ants On Blacklegged Ticks In Acadia National Park, Lucy Guarnieri

Honors College

The blacklegged tick (Ixodes scapularis) and the European fire ant (Myrmica rubra) are notable invasive pests in Maine, especially across coastal and southern regions. I. scapularis is the primary vector of the bacterium Borrelia burgdorferi, which is the causative agent of Lyme disease, and Maine currently has one of the highest Lyme disease incidence rates in the U.S. Ticks have many natural predators, including ants. This study investigates the effects of M. rubra on I. scapularis abundance and pathogen infection prevalence in Acadia National Park (ANP). I collected ticks by drag-sampling at eight ant-infested sites and eight control sites ...


Geospatial Analysis Of Rickettsial Species In Arkansas, Amy D. Frank, Ashley P.G. Dowling Jan 2019

Geospatial Analysis Of Rickettsial Species In Arkansas, Amy D. Frank, Ashley P.G. Dowling

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Rickettsia species are obligate intracellular, arthropod-borne bacteria with a potential to cause multiple diseases including Rocky Mountain spotted fever (RMSF). Fleas, mites, and ticks serve as vectors for Rickettsia, but ticks are the primary vector of interest. RMSF and other rickettsial diseases have continued to gain importance in both human and veterinary medicine as RMSF is the most common tick-borne disease within the United States according to the Lyme and Tick-Borne Disease Research Center. A statewide citizen science project was utilized to determine the prevalence of Spotted Fever Group (SFG) Rickettsia in Arkansas. This project yielded results in 64 of ...


The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas Jan 2019

The Effects Hot Water Kill Time Has On Dna Degradation And Str Profiling From Lucilia Sericata (Diptera: Calliphoridae) Crop Contents, Nicholas Alexander Haas

Graduate Theses, Dissertations, and Problem Reports

he use of entomological specimens as a source of forensic DNA evidence has been studied since the late 20th century. Currently as it stands, to preserve both the minimum postmortem interval (mPMI) and DNA evidence within fly larvae, two standards of preservation exist. For mPMI, the use of hot water kill with near-boiling water is used to fix larvae size for accurate age estimation. For DNA evidence, the standard technique of preservation is submersion in 70% ethanol. To help reduce the number of standard preservation techniques for practitioners, this study assessed the feasibility of the hot water kill preservation technique ...


Impact Of Native Natural Enemies On Populations Of The Invasive Winter Moth (Operophtera Brumata L) In The Northeast United States, Hannah J. Broadley Oct 2018

Impact Of Native Natural Enemies On Populations Of The Invasive Winter Moth (Operophtera Brumata L) In The Northeast United States, Hannah J. Broadley

Doctoral Dissertations

Invasive insects increasingly affect forested landscapes and have important ecological and economic impacts. My dissertation focuses on population dynamics of winter moth (Operophtera brumata L.), an invasive pest in the northeastern United States. Native to Europe, this is the species’ fourth accidental introduction to North America. The Elkinton lab established the biological control agent Cyzenis albicans across the range of winter moth in the northeastern U.S. Prior research indicates that C. albicans’ ability to control winter moth likely depends on additional mortality from native natural enemies. My dissertation research evaluates the identity and role of natural enemies already present ...


Identification Of Target Genes For Rnai-Mediated Control Of The Twospotted Spider Mite, June-Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba Reddy Palli Oct 2018

Identification Of Target Genes For Rnai-Mediated Control Of The Twospotted Spider Mite, June-Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba Reddy Palli

Entomology Faculty Publications

RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids ...


Clover Root Curculio (Sitona Hispidulus F.), Kaitlin Rim, Steven Price, Ricardo Ramirez Oct 2018

Clover Root Curculio (Sitona Hispidulus F.), Kaitlin Rim, Steven Price, Ricardo Ramirez

All Current Publications

The clover root curculio is an important agricultural pest in forage production systems. Adult beetles feed on the host plant leaves while the damaging larval stage feeds on the roots below ground. Heavy larval feeding has been associated with reduced stand establishment, disruption of nutrient and water uptake, increased secondary plant pathogen infection, decreased winter plant survival, delayed green-up, and reductions in forage quality and yield. Due to the hidden nature of eggs and larvae in the soil, CRC has been overlooked and damage is often misdiagnosed as nutrient deficiencies or pathogens. Historically, applications of chlorinated hydrocarbons, carbamates, and organophosphates ...


Confirming World-Wide Distribution Of An Agriculturally Important Lacewing, Chrysoperla Zastrowi Sillemi, Using Songs, Morphology, Mitochondrial Gene Sequencing, And Phylogenetic Reconstruction, Zoe Mandese Aug 2018

Confirming World-Wide Distribution Of An Agriculturally Important Lacewing, Chrysoperla Zastrowi Sillemi, Using Songs, Morphology, Mitochondrial Gene Sequencing, And Phylogenetic Reconstruction, Zoe Mandese

Honors Scholar Theses

The Chrysoperla carnea-group of green lacewings is a cryptic species complex. Species within the group are morphologically similar, yet isolated from one another via reproductive mating song. Chrysoperla zastrowi, a species within the carnea-group, is currently described with a distribution ranging from South Africa to the Middle East and India. However, recent collections of carnea-group lacewings from Guatemala and California were preliminarily identified as Chrysoperla zastrowi based upon similarities in their vibrational courtship songs. This analysis aims to place six specimens, collected by collaborators in Guatemala, Armenia, Iran, and California, into a pre-existing phylogeny of the Chrysoperla ...


Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams Aug 2018

Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams

Theses and Dissertations

Honey bee populations have been fluctuating within recent years. No one cause has been attributed to colony fluctuations due to the theory that multiple stressors interact with one another to impact colony health. Consequently, microorganisms such as internal parasites of honey bees have been understudied as a contributor to colony health decline.

Molecular diagnostics were utilized to detect the presence of two honey bee trypanosome parasites, Crithidia mellificae and Lotmaria passim, in managed and feral honey bee populations from eight states in the United States (USA). Because studies on trypanosome infections are lacking in the USA, it is important to ...


Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger Jun 2018

Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger

Biology Faculty Publications

Bee viral ecology is a fascinating emerging area of research: viruses exert a range of effects on their hosts, exacerbate the impacts of other environmental stressors, and, importantly, are readily shared across multiple bee species in a community. However, our understanding of bee viral communities is limited, as it is primarily derived from studies of North American and European Apis mellifera populations. Here, we examined viruses in populations of A. mellifera and 11 other bee species from 9 countries, across 5 continents and Oceania. We developed a novel pipeline to rapidly, inexpensively, and robustly screen for bee viruses. This pipeline ...


Identification Of Highly Effective Target Genes For Rnai-Mediated Control Of Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Jian J. Duan, Subba Reddy Palli, Lynne K. Rieske Mar 2018

Identification Of Highly Effective Target Genes For Rnai-Mediated Control Of Emerald Ash Borer, Agrilus Planipennis, Thais B. Rodrigues, Jian J. Duan, Subba Reddy Palli, Lynne K. Rieske

Entomology Faculty Publications

Recent study has shown that RNA interference (RNAi) is efficient in emerald ash borer (EAB), Agrilus planipennis, and that ingestion of double-stranded RNA (dsRNA) targeting specific genes causes gene silencing and mortality in neonates. Here, we report on the identification of highly effective target genes for RNAi-mediated control of EAB. We screened 13 candidate genes in neonate larvae and selected the most effective target genes for further investigation, including their effect on EAB adults and on a non-target organism, Tribolium castaneum. The two most efficient target genes selected, hsp (heat shock 70-kDa protein cognate 3) and shi (shibire), caused up ...