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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Detergent Extraction Of A Presumptive Gating Component From The Voltage-Dependent Sodium Channel, W J. Culp, D T. Mckenzie
Detergent Extraction Of A Presumptive Gating Component From The Voltage-Dependent Sodium Channel, W J. Culp, D T. Mckenzie
Dartmouth Scholarship
A physiologically characterized radiolabeled neurotoxin complex obtained from venom of the scorpion Leiurus quinquestriatus has been used to identify detergent-solubilized presumptive sodium channel components in sucrose gradients. This toxin-binding component is found in extracts prepared from three sources of excitable membrane but appears to be absent from similar extracts prepared from nonexcitable membrane or from Torpedo californica membrane. Procedures that destroy the physiological activity of the Leiurus neurotoxin lead to a corresponding loss of toxin binding to the putative sodium channel component. The major component recognized by the Leiurus toxin sediments at 6.5 S. Scatchard analysis of quantitative binding experiments …
The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross
The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross
Dartmouth Scholarship
We have developed a procedure for selectively enriching a mRNA population for inducible sequences. Other than the induced mRNA species, the population of mRNA in control cells is approximately the same as the mRNA population in induced cells. Cytoplasmic mRNA from control cells is bound to oligo (dT)-cellulose and used as a template for reverse transcriptase, the oligo (dT) serving as a primer. After removing the template mRNAs, the cDNA-cellulose column is used to hybridize a population of mRNAs from induced cells. The non-hybridized poly A + RNAs are greatly enriched in the inducible sequences. We have used this technique …
History Of Animal Experimentation Control In The U.K., Judith E. Hampson
History Of Animal Experimentation Control In The U.K., Judith E. Hampson
Laws and Legislation Collection
The legislative control of the use of animals in experiments in the UK lies in the Cruelty to Animals Act of 1876. Animal Welfare groups and individuals in Britain have pressed for reform of this law almost since its inception 105 years ago, and the British government has recently agreed to bring this legislation up to date. Any new or amended legislation could have far-reaching implications, both for laboratory animal welfare and upon the scientific community and is therefore of considerable importance both in this country and overseas. No proper appreciation of the problem would be possible without reference to …
Eye Movement Related Single-Unit Recording In The Nucleus Reticularis Tegmenti Pontis In The Alert Monkey, William Franklin Crandall Jr.
Eye Movement Related Single-Unit Recording In The Nucleus Reticularis Tegmenti Pontis In The Alert Monkey, William Franklin Crandall Jr.
University of the Pacific Theses and Dissertations
Nucleus Reticularis Tegmenti Pontis (NRTP) is a structure which lies in the mid-brain ventral to the oculomotor complex. Anatomical studies strongly implicate it in oculomotor function as most of its inputs and outputs are to systems previously shown to be intimately involved in eye movement generation--superior colliculus (SC) and cerebellum. These reports show an integrative structure with inputs and outputs distributed in a way well suited for performing relay and feedback tasks. Physiological data in the alert, trained monkey has been gathered in an attempt to characterize these neurons and to classify them in functional terms. Three monkeys were trained …
Utilization Of Aspen Populus-Tremuloides Trees As A Ruminant Feed Component, M. Singh, L.D. Kamstra
Utilization Of Aspen Populus-Tremuloides Trees As A Ruminant Feed Component, M. Singh, L.D. Kamstra
Aspen Bibliography
When traditional roughages are in short supply or high in price, other fibrous feed sources for ruminants should be considered if available at a competitive price. One such fibrous material shown to have potential as a ruminant feed is the aspen tree (Populus tremuloides Michx.) harvested in its entire form to include bark, leaves and trunk. The aspen tree is the most widespread tree species in North America and the least utilized. Estimates exceed 6 million acres of mature trees in an area which would include the Black Hills (58,000 acres), the Great Lakes region and the Rocky …