Life Sciences Commons^™

Phylogenetic Investigation Of Enteric Bovine Coronavirus In Ireland Reveals Partitioning Between European And Global Strains, Lynda Gunn, P. J. Collins, M. J. O'Connell, Helen O'Shea

Department of Biological Sciences Publications

Background

Bovine coronavirus is a primary cause of neonatal calf diarrhea worldwide, and is also associated with acute diarrhea in adult cattle during the winter season. There are no reports on molecular characterization of bovine coronavirus in Ireland, and little data exists apart from serological studies.

Findings

In this study, 11 neonatal (mean age 9 days) calf BCoV strains from the south of Ireland were collected over a one year period and characterized using molecular methods. The spike gene which encodes a protein involved in viral entry, infectivity and immune response shows the most variability amongst the isolates and was …

Thinking Beyond The Fried Egg Model: How Accurately Do Students Perceive Cells In A Living Context?, Milissa Knox

Electronic Theses and Dissertations

This exploratory study investigated three aspects of introductory undergraduate biology students’ understanding about cells. The study, which took place at the University of Maine with voluntary students in Basic Biology (“BIO100”) in the summer and fall of 2009, examined (1) students’ pre-course perceptions of cells as they exist in a living context and (2) gains in students’ perception and knowledge about cells after completing the one-semester course (BIO100). Results are based on lecture exam scores, pre-post surveys developed as a part of this thesis, and interviews with two groups of biology students. A total of 498 students participated in the …

The Role Of Valproic Acid, A Histone Deacetylase Inhibitor, In Reducing Anxiety Levels In Rats: An Epigenetics Study, Juliet Buteme

Honors College Theses

Rats, like humans, show personality traits: they are born with different anxiety levels (i.e., high (HAn) or low anxiety (LAn) levels). In this study, we investigated (1) if treatment with valproic acid (VPA) can lower anxiety level in trait anxiety rats and (2) how VPA may interact with environment (e.g., enriched (EE), standard (SE) and isolated environments (IE)). VPA is a deacetylase inhibitor that increases Histone 3 acetylation, a known epigenetic mechanism that interacts with stress response proteins, and treats epilepsy and mood disorders. Since rats reared in IE exhibit heightened anxiety levels as compared to those in EE, we …

Territorial Defense Strategies In The Northern Cardinal (Cardinalis Cardinalis): Who Is The Bigger Threat?, Kaylee M. Gentry

Master's Theses

This thesis examines the use of defensive strategies in relation to territories year round in the northern cardinal (Cardinalis cardinalis). Responses to recorded neighbor song and stranger song playback from the middle of a focal male’s territory were measured. This allowed for an estimation of aggression in both the winter and spring seasons. Each focal male was subjected to both treatments (stranger song and neighbor song). Males were more responsive over-all to neighbor song playback, however in the winter months, persistence of response to neighbor song playback increased. It was also shown that southeastern United States cardinals show …

Estimation Problems In Complex Field Studies With Deep Interactions: Time-To-Event And Local Regression Models For Environmental Effects On Vital Rates, Krzysztof M. Sakrejda

Doctoral Dissertations

Field studies that measure vital rates in context over extended time periods are a cornerstone of our understanding of population processes. These studies inform us about the relationship between biological process and environmental noise in an irreplaceable way. These data sets bring ``big data'' and ``big model'' challenges, which limit the application of standard software (e.g., \textbf{BUGS}). The environmental sensitivity of vital rates is also expected to exhibit interactions and non-linearity, which typically result in difficult model selection questions in large data sets. Finally, long-term ecological data sets often contain complex temporal structure. In commonly applied discrete-time models complex temporal …

Serum Proteins Enhance Dispersion Stability And Influence The Cytotoxicity And Dosimetry Of Zno Nanoparticles In Suspension And Adherent Cancer Cell Models, Catherine B. Anders, Jordan J. Chess, Denise G. Wingett, Alex Punnoose

Biomolecular Research Center Publications and Presentations

Agglomeration and sedimentation of nanoparticles (NPs) within biological solutions is a major limitation in their use in many downstream applications. It has been proposed that serum proteins associate with the NP surface to form a protein corona that limits agglomeration and sedimentation. Here, we investigate the effect of fetal bovine serum (FBS) proteins on the dispersion stability, dosimetry, and NP-induced cytotoxicity of cationic zinc oxide nanoparticles (nZnO) synthesized via forced hydrolysis with a core size of 10 nm. Two different in vitro cell culture models, suspension and adherent, were evaluated by comparing a phosphate buffered saline (PBS) nZnO dispersion (nZnO/PBS) …

Evolution Practice 2, Imsa Biology Team

Evolution

Evolution Problems

Genetic Drift Simulation, Imsa Biology Team

Evolution

Genetic drift can be defined as a random fluctuation in gene frequency. More specifically, it tells us that different alleles may increase or decrease in a population in proportion to one another over time, just by chance, rather than due to any fitness advantage.

Mechanisms And Speciation 2: Evolution On The Web Questions, Imsa Biology Team

Evolution

The website below, sponsored by UC Berkeley, is a reliable source for information about evolution. This will give you the introductory information about the mechanisms of Evolution.

Mechanisms And Speciation 1: The Modern Synthesis, Imsa Biology Team

Evolution

The Modern Synthesis or Synthetic Theory of Evolution is an explanation of evolution that is based on modern genetic principles. According to the Modern Synthesis (a.k.a. Neo-Darwinism):

Evidence Of Evolution 2: Definitions For Evolutionary Evidence Lab, Imsa Biology Team

Evolution

This lab activity was designed to provide you with opportunities to make inferences and draw conclusions about evolution and the common ancestry of various animals based on their anatomical characteristics and comparative anatomy. You will be given tasks to accomplish or questions to answer at each of 11 stations. In order to do this successfully, you must make careful observations of the specimens on display. You must also know the following definitions before beginning this activity:

Evolution Practice 1, Imsa Biology Team

Evolution

Answer the following questions using information from the handouts, and examples from the LAB

Evidence Of Evolution 1: Structures, Imsa Biology Team

Evolution

In order to investigate linage, evolutionary history, and common ancestry, you need to be familiar with the following structures.

Essential Questions, Imsa Biology Team

Evolution

In order to understand the current state of biological life and how it has changed over time, the following questions must be addressed:

Evidence Of Evolution 3: Evolutionary Evidence And Inferences Lab: A Discussion Guide, Imsa Biology Team

Evolution

This lab activity was designed to provide you with opportunities to make inferences and draw conclusions about evolution and the common ancestry of various animals based on their anatomical characteristics and comparative anatomy. You will be given tasks to accomplish or questions to answer at each of 11 stations. In order to do this successfully, you must make careful observations of the specimens on display.

Sequencing Techniques: A Comparison Assignment, Sarah O'Leary-Driscoll

Sequencing & Genome Mining

With your partner, create some sort of visual (table, map, chart, other, ask me!) that compares the main types of sequencing that we discussed, as well as two of the techniques considered 'next generation'.

Discussion Questions: Genome Mining, Sarah O'Leary-Driscoll

Sequencing & Genome Mining

No abstract provided.

Alignment Information, Sarah O'Leary-Driscoll

Sequence Alignments

Pairwise DNA alignment is frequently used to identify similar regions that will show how two sequences have functional or structural similarities. It can also be used to show how exons and introns change between different sequences and whether they have an effect on the final structure of the RNA after the DNA is processed within a cell.

Alignment Outline, Sarah O'Leary-Driscoll

Sequence Alignments

No abstract provided.

2: Sequence Alignment Practice Activity, Sarah O'Leary-Driscoll

Sequence Alignments

Now that you have learned how to do the four basic sequence alignments (Pairwise and Multiple for both nucleotide and protein sequences) select a gene/protein, it may be one that you've used before, and run each of these alignments.

Pt. 2: Presentation / Paper Guidelines, Sarah O'Leary-Driscoll

Research Project

The presentations for your project should follow the same format that the paper would, but in a much more abbreviated form, aim for 5-7 minutes.

Project Guidelines, Sarah O'Leary-Driscoll

Research Project

No abstract provided.

Pt. 1: Research Question & Background, Sarah O'Leary-Driscoll

Research Project

No abstract provided.

Primer Design Activity, Sarah O'Leary-Driscoll

Primer Design

No abstract provided.

Obtaining Genomic Sequence Practice, Sarah O'Leary-Driscoll

Introduction to NCBI

No abstract provided.

Dna Timeline And Poster Project, Sarah O'Leary-Driscoll

Genomics: Past & Future

The DNA timeline goes through many of the major discoveries that have driven our understanding of genetics since Mendel. Pick two scientists and create a PowerPoint slide poster (to be printed out on regular printer sized paper) that covers the following:

3: Genomics: Past & Future Bibliography, Sarah O'Leary-Driscoll

Genomics: Past & Future

No abstract provided.

Future Of Genomics: Presentations, Sarah O'Leary-Driscoll

Genomics: Past & Future

In his testimony to a House of Representatives sub-committee on health, director of the National Human Genome Research Institute, Francis S. Collins, said that the future of genomics had three main focal points:

"Genomics to Biology: The human genome sequence provides foundational information that now will allow development of a comprehensive catalog of all of the genome's components, determination of the function of all human genes, and deciphering of how genes and proteins work together in pathways and networks.

Genomics to Health: Completion of the human genome sequence offers a unique opportunity to understand the role of genetic factors in …

Database/Resource Acronyms, Sarah O'Leary-Driscoll

Course Information

No abstract provided.

What Is Bioinformatics?, Sarah O'Leary-Driscoll

Course Information

Bioinformatics has evolved into a full-fledged multidisciplinary subject that integrates developments in information and computer technology as applied to Biotechnology and Biological Sciences. Bioinformatics uses computer software tools for database creation, data management, data warehousing, data mining and global communication networking. Bioinformatics is the recording, annotation, storage, analysis, and searching/retrieval of nucleic acid sequence (genes and RNAs), protein sequence and structural information. This includes databases of the sequences and structural information as well methods to access, search, visualize and retrieve the information. Bioinformatics concern the creation and maintenance of databases of biological information whereby researchers can both access existing information …