Life Sciences Commons^™

Ua1c11/55 Gordon Wilson Photo Collection, Wku Archives

WKU Archives Collection Inventories

Photographs taken by and of Gordon Wilson.

The Status Of Fishes In The Missouri River, Nebraska: Shoal Chub (Macrhybopsis Hyostoma), Sturgeon Chub (M. Gelida), Sicklefin Chub (M. Meeki), Silver Chub (M. Storeriana), Flathead Chub (Platygobio Gracilis), Plains Minnow (Hybognathus Placitus), Western Silvery Minnow (H. Argyritis), And Brassy Minnow (H. Hankinsoni), Kirk D. Steffensen, Dane A. Shuman, Sam Stukel

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Throughout the past century, the Missouri River has been highly modified which has negatively affected the native fish community. Previous research quantified the reduction of several native Cyprinidae species and made several recommendations to aid in recovery. However, these recommendations were not implemented. Therefore, the objective of this paper is to assess the current population trends of eight native cyprinid species and develop the current management objectives. Over 335,000 fish were collected from the Missouri River along Nebraska’s eastern border with mini-fyke nets and otter trawls from 2003 to 2012. Target Cyprinidae species consisted of less than five percent (n …

Identifying Glioblastoma Gene Networks Based On Hypergeometric Test Analysis, Vasileios Stathias, Chiara Pastori, Tess Z. Griffin, Ricardo Komotar, Jennifer L. Clarke, Ming Zhang, Nagi G. Ayad

Department of Statistics: Faculty Publications

Patient specific therapy is emerging as an important possibility for many cancer patients. However, to identify such therapies it is essential to determine the genomic and transcriptional alterations present in one tumor relative to control samples. This presents a challenge since use of a single sample precludes many standard statistical analysis techniques. We reasoned that one means of addressing this issue is by comparing transcriptional changes in one tumor with those observed in a large cohort of patients analyzed by The Cancer Genome Atlas (TCGA). To test this directly, we devised a bioinformatics pipeline to identify differentially expressed genes in …

Fish Sound Production In The Presence Of Harmful Algal Blooms In The Eastern Gulf Of Mexico, Carrie C. Wall, Chad Lembke, Chuanmin Hu, David A. Mann

Marine Science Faculty Publications

This paper presents the first known research to examine sound production by fishes during harmful algal blooms (HABs). Most fish sound production is species-specific and repetitive, enabling passive acoustic monitoring to identify the distribution and behavior of soniferous species. Autonomous gliders that collect passive acoustic data and environmental data concurrently can be used to establish the oceanographic conditions surrounding sound-producing organisms. Three passive acoustic glider missions were conducted off west-central Florida in October 2011, and September and October 2012. The deployment period for two missions was dictated by the presence of red tide events with the glider path specifically set …

The Status Of Fishes In The Missouri River, Nebraska: Sauger (Sander Canadensis), Kirk D. Steffensen, Sam Stukel, Dane A. Shuman

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Early fisheries investigation of the Missouri River fish community indicated that Sauger were common throughout Nebraska, including all major Missouri River tributaries. However due to many factors, their current range is restricted to the Missouri River and the lower reaches of a few tributaries. Hesse (1994) recommended listing Sauger as a state endangered species but this recommendation was never implemented. Therefore, the objective of this paper is to reevaluate the current population status of Sauger in the Missouri River along Nebraska’s border. Over 2,100 Sauger have been captured from the Missouri River along Nebraska’s eastern border since 2003. Sauger were …

U.S. Drought Monitor, December 30, 2014, Brad Rippey

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 30, 2014 (12/30/14) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Evolutionary Genomics And Adaptive Evolution Of The Hedgehog Gene Family (Shh, Ihh And Dhh) In Vertebrates, Joana Pereira, Warren E. Johnson, Stephen J. O'Brien, Erich D. Jarvis, Guojie Zhang, M. Thomas P. Gilbert, Vitor Vasconcelos, Agostinho Antunes

Biology Faculty Articles

The Hedgehog (Hh) gene family codes for a class of secreted proteins composed of two active domains that act as signalling molecules during embryo development, namely for the development of the nervous and skeletal systems and the formation of the testis cord. While only one Hh gene is found typically in invertebrate genomes, most vertebrates species have three (Sonic hedgehog – Shh; Indian hedgehog – Ihh; and Desert hedgehog – Dhh), each with different expression patterns and functions, which likely helped promote the increasing complexity of vertebrates and their successful diversification. In this study, we …

American Society Of Parasitologists Newsletter, V. 36, Nos. 3-4, Fall-Winter 2014, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

No abstract provided.

Modelling Non-Euclideanmovement And Landscape Connectivity In Highly Structured Ecological Networks, Chris Sutherland, Angela Fuller, J. Royle

Environmental Conservation Faculty Publication Series

1. Movement is influenced by landscape structure, configuration and geometry, but measuring distance as perceived by animals poses technical and logistical challenges. Instead, movement is typically measured using Euclidean distance, irrespective of location or landscape structure, or is based on arbitrary cost surfaces. Arecently proposed extension of spatial capture-recapture (SCR)models resolves this issue using spatial encounterhistories of individuals to calculate least-cost paths (ecological distance: Ecology, 94, 2013, 287) thereby relaxingthe Euclidean assumption. We evaluate the consequences of not accounting for movement heterogeneity whenestimating abundance in highly structured landscapes, and demonstrate the value of this approach for estimatingbiologically realistic space-use patterns …

Integration Of Developmental And Environmental Signals Via A Polyadenylation Factor In Arabidopsis, Man Liu, Ruqiang Xu, Carrie Merrill, Liwei Hong, Carol Von Lanken, Arthur G. Hunt, Qingshun Q. Li

Plant and Soil Sciences Faculty Publications

The ability to integrate environmental and developmental signals with physiological responses is critical for plant survival. How this integration is done, particularly through posttranscriptional control of gene expression, is poorly understood. Previously, it was found that the 30 kD subunit of Arabidopsis cleavage and polyadenylation specificity factor (AtCPSF30) is a calmodulin-regulated RNA-binding protein. Here we demonstrated that mutant plants (oxt6) deficient in AtCPSF30 possess a novel range of phenotypes--reduced fertility, reduced lateral root formation, and altered sensitivities to oxidative stress and a number of plant hormones (auxin, cytokinin, gibberellic acid, and ACC). While the wild-type AtCPSF30 (C30G) was …

Tensile Testing To Quantitate The Anisotropy And Strain Hardening Of Mozzarella Cheese, Ramona Bast, Prateek Sharma, Hannah K.B. Easton, Tzvetelin T. Dessev, Mita Lad, Peter A. Munro

Nutrition, Dietetics, and Food Sciences Faculty Publications

We explored anisotropy of mozzarella cheese: its presence is debated in the literature. Tensile testing proved a good method because the location and mode of failure were clear. Mozzarella cheese cut direct from the block showed no significant anisotropy, though confocal microscopy showed good structure alignment at a microscale. Deliberately elongated mozzarella cheese showed strong anisotropy with tensile strength in the elongation or fibre direction ∼3.5× that perpendicular to the fibres. Temperature of elongation had a marked impact on anisotropy with maximum anisotropy after elongation at 70 °C. We suggest the disagreement on anisotropy in the literature is related to …

Amylin Structure, Aggregation, And Pancreatic Β Cell Toxicity, Sharadrao Patil

MCB Articles

In most type 2 diabetes patients, amyloid plaques have been found juxtaposed with membranes of pancreatic β-cells. These plaques are composed of amyloid fibrils of the 37 residue endocrine hormone amylin and cause distinct changes in cell membrane morphology associated with the destruction of β-cells. Research is still ongoing to identify the toxic species involved and the mechanisms by which mature fibrils or oligomers cause cytotoxicity. The projects undertaken were designed to study the molecular structural features of amylin, mechanism of amyloid aggregation and, to develop cytotoxicity inhibitors. We determined the structure of human amylin bound to SDS micelles using …

Baseline Survey For Street Dogs In Guam, John D. Boone

Stray and Feral Animal Populations Collection

As is the case for many inhabited islands in Pacific region, dogs are common, including freeroaming street dogs that may be owned or unowned. Reproduction among dogs is largely unchecked, and many owned dogs do not receive adequate levels of basic care in the home or veterinary care when needed. For all of these reasons, Humane Society International (HSI) is exploring options for improving the management of street dog populations and promoting better treatment and care of all dogs on Guam and on the nearby islands of Rota and Saipan. One of the initial steps in this process was to …

Winter Activity Of Bats In Southeastern Nebraska: An Acoustic Study, Jeremy A. White, Brett R. Andersen, Hans W. Otto, Cliff A. Lemen, Patricia W. Freeman

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Many North American bats are active outside hibernacula in winter, but no information on winter activity has been reported for Nebraska. We recorded activity of bats during two winters (December-February 2012-2013 and 2013-2014) at one location in southeastern Nebraska with an acoustic detector. Bats were active throughout both winters and temperature at sunset was a good predictor of bat activity. Red bats (Lasiurus borealis) were active at our site in early December but were not recorded later in winter. We suspect these individuals were late migrants to more southern wintering sites. Big brown bats (Eptesicus fuscus) …

High Rates Of Hiv Seroconversion In Pregnant Women And Low Reported Levels Of Hiv Testing Among Male Partners In Southern Mozambique: Results From A Mixed Methods Study, Caroline De Schacht, Heather J. Hoffman, Nedio Mabunda, Carlota Lucas, Catharina L. Alons, Ana Madonela, Adolfo Vubil, Orlando C. Ferreira, Nurbai Calu, Iolanda S. Santos, Ilesh V. Jani, Laura Guay

Genomics and Precision Medicine Faculty Publications

Introduction

Prevention of acute HIV infections in pregnancy is required to achieve elimination of pediatric HIV. Identification and support for HIV negative pregnant women and their partners, particularly serodiscordant couples, are critical. A mixed method study done in Southern Mozambique estimated HIV incidence during pregnancy, associated risk factors and factors influencing partner's HIV testing.

Methods

Between April 2008 and November 2011, a prospective cohort of 1230 HIV negative pregnant women was followed during pregnancy. A structured questionnaire, HIV testing, and collection of dried blood spots were done at 2–3 scheduled visits. HIV incidence rates were calculated by repeat HIV testing …

Using Plant Volatile Traps To Develop Phenology Models For Natural Enemies: An Example Using Chrysopa Nigricornis (Burmeister) (Neuroptera: Chrysopidae), Vincent P. Jones, David R. Horton, Nicholas J. Mills, Thomas R. Unruh, Eugene R. Milickzy, Peter W. Shearer, Kaushalya G. Amarasekare, Callie C. Baker, Tawnee D. Melton

Agricultural and Environmental Sciences Faculty Research

A model predicting phenology of adult Chrysopa nigricornis (Burmeister) (Neuroptera: Chrysopidae) in orchards was developed from field (trapping) data supplemented with development data collected under laboratory conditions. Lower and upper thresholds of 10.1°C and 29.9°C, respectively, were estimated from published and unpublished laboratory observations, and were used to develop a phenology model. Season-long field data were collected using white delta traps that had been baited with squalene, a volatile shown previously to be highly attractive to C. nigricornis. The model was developed from data collected in three Washington apple orchards, and was validated using independent data sets collected from apple, …

Real-Time Kinetic Studies Of Bacillus Subtilis Oxalate Decarboxylase And Ceriporiopsis Subvermispora Oxalate Oxidase Using Luminescent Oxygen Sensor, Laura Molina, Thomas Goodall, Umar Twahir, Ellen W. Moomaw

Faculty and Research Publications

Oxalate decarboxylase (OxDC), an enzyme of the bicupin superfamily, catalyzes the decomposition of oxalate into carbon dioxide and formate at an optimal pH of 4.3 in the presence of oxygen. However, about 0.2% of all reactions occur through an oxidase mechanism that consumes oxygen while producing two equivalents of carbon dioxide and one equivalent of hydrogen peroxide. The kinetics of oxidase activity were studied by measuring the consumption of dissolved oxygen over time using a luminescent oxygen sensor. We describe the implementation of and improvements to the oxygen consumption assay. The oxidase activity of wild type OxDC was compared to …

Molecular Targets Of Antihypertensive Peptides: Understanding The Mechanisms Of Action Based On The Pathophysiology Of Hypertension, Kaustav Majumder, Jianping Wu

Department of Food Science and Technology: Faculty Publications

There is growing interest in using functional foods or nutraceuticals for the prevention and treatment of hypertension or high blood pressure. Although numerous preventive and therapeutic pharmacological interventions are available on the market, unfortunately, many patients still suffer from poorly controlled hypertension. Furthermore, most pharmacological drugs, such as inhibitors of angiotensin-I converting enzyme (ACE), are often associated with significant adverse effects. Many bioactive food compounds have been characterized over the past decades that may contribute to the management of hypertension; for example, bioactive peptides derived from various food proteins with antihypertensive properties have gained a great deal of attention. Some …

Feather Corticosterone Reveals Effect Of Moulting Conditions In The Autumn On Subsequent Reproductive Output And Survival In An Arctic Migratory Bird, N. Jane Harms, Pierre Legagneux, H. Grant Gilchrist, Joël Bêty, Oliver P. Love, Mark R. Forbes, Gary R. Bortolotti, Catherine Soos

Integrative Biology Publications

For birds, unpredictable environments during the energetically stressful times of moulting and breeding are expected to have negative fitness effects. Detecting those effects however, might be difficult if individuals modulate their physiology and/or behaviours in ways to minimize short-term fitness costs. Corticosterone in feathers (CORTf) is thought to provide information on total baseline and stress-induced CORT levels at moulting and is an integrated measure of hypothalamic-pituitary-adrenal activity during the time feathers are grown.We predicted that CORTf levels in northern common eider females would relate to subsequent body condition, reproductive success and survival, in a population of eiders nesting in the …

Predicted Changes In Climatic Niche And Climate Refugia Of Conservation Priority Salamander Species In The Northeastern United States, William B. Sutton, Kyle Barrett, Allison T. Moody, Cynthia S. Loftin, Phillip G. Demaynadier, Priya Nanjappa

Agricultural and Environmental Sciences Faculty Research

Global climate change represents one of the most extensive and pervasive threats to wildlife populations. Amphibians, specifically salamanders, are particularly susceptible to the effects of changing climates due to their restrictive physiological requirements and low vagility; however, little is known about which landscapes and species are vulnerable to climate change. Our study objectives included, (1) evaluating species-specific predictions (based on 2050 climate projections) and vulnerabilities to climate change and (2) using collective species responses to identify areas of climate refugia for conservation priority salamanders in the northeastern United States. All evaluated salamander species were projected to lose a portion of …

Functional And Developmental Identification Of A Molecular Subtype Of Brain Serotonergic Neuron Specialized To Regulate Breathing Dynamics, Rachael D. Brust, Andrea E. Corcoran, George B. Richerson, Eugene Nattie, Susan M. Dymecki

Dartmouth Scholarship

Serotonergic neurons modulate behavioral and physiological responses from aggression and anxiety to breathing and thermoregulation. Disorders involving serotonin (5HT) dysregulation are commensurately heterogeneous and numerous. We hypothesized that this breadth in functionality derives in part from a developmentally determined substructure of distinct subtypes of 5HT neurons each specialized to modulate specific behaviors. By manipulating developmentally defined subgroups one by one chemogenetically, we find that the Egr2-Pet1 subgroup is specialized to drive increased ventilation in response to carbon dioxide elevation and acidosis. Furthermore, this subtype exhibits intrinsic chemosensitivity and modality-specific projections-increasing firing during hypercapnic acidosis and selectively projecting to respiratory chemosensory …

Spatial Clustering Of Physical Activity And Obesity In Relation To Built Environment Factors Among Older Women In Three U.S. States, Kosuke Tamura, Robin C. Puett, Jaime E. Hart, Heather A. Starnes, Francine Laden, Philip J. Troped

Kinesiology and Public Health

Background

Identifying spatial clusters of chronic diseases has been conducted over the past several decades. More recently these approaches have been applied to physical activity and obesity. However, few studies have investigated built environment characteristics in relation to these spatial clusters. This study’s aims were to detect spatial clusters of physical activity and obesity, examine whether the geographic distribution of covariates affects clusters, and compare built environment characteristics inside and outside clusters.

Methods

In 2004, Nurses’ Health Study participants from California, Massachusetts, and Pennsylvania completed survey items on physical activity (N = 22,599) and weight-status (N = 19,448). The spatial …

Interplanting Annual Ryegrass, Wheat, Oat, And Corn To Mitigate Iron Deficiency In Dry Beans, Emmanuel Chiwo Omondi, Andrew R. Kniss

Agricultural and Environmental Sciences Faculty Research

This study evaluated whether grass intercropping can be used to alleviate Fe deficiency chlorosis in dry beans (Phaseolus vulgaris L.) grown in high pH, calcareous soils with low organic matter. Field studies were conducted at the University of Wyoming Sustainable Agriculture Research and Extension Center in 2009 and 2010. Black- and navy beans were grown alone or intercropped with annual ryegrass (Lolium multiflorum Lam.), oat (Avena sativa L.), corn (Zea mays L.), or spring wheat (Triticum aestivum L.) in a two-factor factorial strip-plot randomized complete block design. All four grass species increased chlorophyll intensity in dry beans. However, grass species …

Ontogenetic Scaling Patterns And Functional Anatomy Of The Pelvic Limb Musculature In Emus (Dromaius Novaehollandiae), Luis P. Lamas, Russell P. Main, John R. Hutchinson

Department of Basic Medical Sciences Faculty Publications

Emus (Dromaius novaehollandiae) are exclusively terrestrial, bipedal and cursorial ratites with some similar biomechanical characteristics to humans. Their growth rates are impressive, as their body mass increases eighty-fold from hatching to adulthood whilst maintaining the same mode of locomotion throughout life. These ontogenetic characteristics stimulate biomechanical questions about the strategies that allow emus to cope with their rapid growth and locomotion, which can be partly addressed via scaling (allometric) analysis of morphology. In this study we have collected pelvic limb anatomical data (muscle architecture, tendon length, tendon mass and bone lengths) and calculated muscle physiological cross sectional area …

Indole Alkaloids From Fischerella Inhibit Vertebrate Development In The Zebrafish (Danio Rerio) Embryo Model, Katherine Walton, Miroslav Gantar, Patrick D.L. Gibbs, Michael C. Schmale, John P. Berry

Department of Chemistry and Biochemistry

Cyanobacteria are recognized producers of toxic or otherwise bioactive metabolite associated, in particular, with so-called “harmful algal blooms” (HABs) and eutrophication of freshwater systems. In the present study, two apparently teratogenic indole alkaloids from a freshwater strain of the widespread cyanobacterial genus, Fischerella (Stigonemataceae), were isolated by bioassay-guided fractionation, specifically using the zebrafish (Danio rerio) embryo, as a model of vertebrate development. The two alkaloids include the previously known 12-epi-hapalindole H isonitrile (1), and a new nitrile-containing variant, 12-epi-ambiguine B nitrile (2). Although both compounds were toxic to developing embryos, the former compound was shown to be relatively more potent, …

Mtorc2 Is Required For Rit-Mediated Oxidative Stress Resistance, Weikang Cai, Douglas A. Andres

Molecular and Cellular Biochemistry Faculty Publications

Rit, a member of the Ras family of GTPases, has been shown to promote cell survival in response to oxidative stress, in part by directing an evolutionarily conserved p38 MAPK-Akt survival cascade. Aberrant Rit signaling has recently been implicated as a driver mutation in human cancer, adding importance to the characterization of critical Rit effector pathways. However, the mechanism by which Rit-p38 signaling regulated Akt activity was unknown. Here, we identify mTORC2 as a critical downstream mediator of Rit-dependent survival signaling in response to reactive oxygen species (ROS) stress. Rit interacts with Sin1 (MAPKAP1), and Rit loss compromises ROS-dependent mTORC2 …

Gut Microbiota-Derived Short-Chain Fatty Acids, T Cells, And Inflammation, Chang Kim, Jeongho Park, Myunghoo Kim

Department of Comparative Pathobiology Faculty Publications

T cells are central players in the regulation of adaptive immunity and immune tolerance. In the periphery, T cell differentiation for maturation and effector function is regulated by a number of factors. Various factors such as antigens, co-stimulation signals, and cytokines regulate T cell differentiation into functionally specialized effector and regulatory T cells. Other factors such as nutrients, micronutrients, nuclear hormones and microbial products provide important environmental cues for T cell differentiation. A mounting body of evidence indicates that the microbial metabolites short-chain fatty acids (SCFAs) have profound effects on T cells and directly and indirectly regulate their differentiation. We …

Broadband In Nebraska: Current Landscape And Recommendations, Nebraska Information Technology Commission, Nebraska Broadband Initiative, Nebraska Public Service Commission, University Of Nebraska-Lincoln, Nitc Community Council, Nebraska Department Of Economic Development, Aim

Rural Futures Institute: Publications

N ebraska’s broadband vision is that residents, businesses, government entities, commu-nity partners, and visitors have access to affordable broadband service and have the necessary skills to effectively utilize broadband technologies.

Objectives

To increase economic development opportunities, create good-paying jobs, at-tract and retain population, overcome the barriers of distance, and enhance qual-ity of life in Nebraska by stimulating the continuing deployment of broadband technologies which meet the need for increasing connection speeds.

To increase digital literacy and the widespread adoption of broadband technolo-gies in business, agriculture, health care, education, government and by individu-al Nebraskans.

Goals

The following goals and targets help …

Effects Of Barbell Deadlift Training On Submaximal Motor Unit Firing Rates For The Vastus Lateralis And Rectus Femoris, Matt S. Stock, Brennan J. Thompson

Kinesiology and Health Science Faculty Publications

Previous investigations that have studied motor unit firing rates following strength training have been limited to small muscles, isometric training, or interventions involving exercise machines. We examined the effects of ten weeks of supervised barbell deadlift training on motor unit firing rates for the vastus lateralis and rectus femoris during a 50% maximum voluntary contraction (MVC) assessment. Twentyfour previously untrained men (mean age 524 years) were randomly assigned to training (n515) or control (n59) groups. Before and following the intervention, the subjects performed isometric testing of the right knee extensors while bipolar surface electromyographic signals were detected from the two …

Investigating The Links Between Ozone And Organic Aerosol Chemistry In A Biomass Burning Plume From A Prescribed Fire In California Chaparral, M. J. Alvarado, C. R. Lonsdale, Robert Yokelson, S. K. Akagi, H. Coe, J. S. Craven, E. V. Fischer, G. R. Mcmeeking, J. H. Seinfeld, T. Soni, J. W. Taylor, D. R. Weise, C. E. Wold

Chemistry and Biochemistry Faculty Publications

Within minutes after emission, rapid, complex photochemistry within a biomass burning smoke plume can cause large changes in the concentrations of ozone (O₃) and organic aerosol (OA). Being able to understand and simulate this rapid chemical evolution under a wide variety of conditions is a critical part of forecasting the impact of these fires on air quality, atmospheric composition, and climate. Here we use version 2.1 of the Aerosol Simulation Program (ASP) to simulate the evolution of O₃ and secondary organic aerosol (SOA) within a young biomass burning smoke plume from the Williams prescribed burn in chaparral, …