Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 30 of 51

Full-Text Articles in Life Sciences

Metabolic Engineering Of Oilseed Crops To Produce High Levels Of Novel Acetyl Glyceride Oils With Reduced Viscosity, Freezing Point And Calorific Value, Jinjie Liu, Adam Rice, Kathleen Mcglew, Vincent Shaw, Hyunwoo Park, Thomas E. Clemente, Mike Pollard, John Ohlrogge, Timothy P. Durrett Dec 2014

Metabolic Engineering Of Oilseed Crops To Produce High Levels Of Novel Acetyl Glyceride Oils With Reduced Viscosity, Freezing Point And Calorific Value, Jinjie Liu, Adam Rice, Kathleen Mcglew, Vincent Shaw, Hyunwoo Park, Thomas E. Clemente, Mike Pollard, John Ohlrogge, Timothy P. Durrett

Agronomy & Horticulture -- Faculty Publications

Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2- diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina ...


2015 Spring Seed Guide, Teshome Regassa, Charles A. Shapiro Dec 2014

2015 Spring Seed Guide, Teshome Regassa, Charles A. Shapiro

Agronomy & Horticulture -- Faculty Publications

NEBRASKA CORN HYBRID TESTS CROP PRODUCTION SUMMARY: According to the National Agricultural Statistics Service, there were 8.75 million acres of corn harvested in Nebraska in 2014 producing approximately 1.58 billion bushels of grain. The total average corn yield for Nebraska in 2014 was a record 181 bushels per acre (bu/a). Total corn yields from the previous 10 years are reported below.

NEBRASKA SOYBEAN VARIETY TESTS - 2014 - CROP PRODUCTION SUMMARY: According to the National Agricultural Statistics Service, there were 5.4 million acres of soybeans planted in Nebraska in 2014. 5.35 million acres were harvested producing around ...


Functional Genomics And Microbiome Profiling Of The Asian Longhorned Beetle (Anoplophora Glabripennis) Reveal Insights Into The Digestive Physiology And Nutritional Ecology Of Wood Feeding Beetles, Erin D. Scully, Scott M. Geib, John E. Carlson, Ming Tien, Duane Mckenna, Kelli Hoover Dec 2014

Functional Genomics And Microbiome Profiling Of The Asian Longhorned Beetle (Anoplophora Glabripennis) Reveal Insights Into The Digestive Physiology And Nutritional Ecology Of Wood Feeding Beetles, Erin D. Scully, Scott M. Geib, John E. Carlson, Ming Tien, Duane Mckenna, Kelli Hoover

Agronomy & Horticulture -- Faculty Publications

Background: Wood-feeding beetles harbor an ecologically rich and taxonomically diverse assemblage of gut microbes that appear to promote survival in woody tissue, which is devoid of nitrogen and essential nutrients. Nevertheless, the contributions of these apparent symbionts to digestive physiology and nutritional ecology remain uncharacterized in most beetle lineages.

Results: Through parallel transcriptome profiling of beetle- and microbial- derived mRNAs, we demonstrate that the midgut microbiome of the Asian longhorned beetle (Anoplophora glabripennis), a member of the beetle family Cerambycidae, is enriched in biosynthetic pathways for the synthesis of essential amino acids, vitamins, and sterols. Consequently, the midgut microbiome of ...


Comprehensive Characterization And Rna-Seq Profiling Of The Hd-Zip Transcription Factor Family In Soybean (Glycine Max) During Dehydration And Salt Stress, Vikas Belamkar, Nathan T. Weeks, Arvind K. Bharti, Andrew Farmer, Michelle A. Graham, Steven B. Cannon Nov 2014

Comprehensive Characterization And Rna-Seq Profiling Of The Hd-Zip Transcription Factor Family In Soybean (Glycine Max) During Dehydration And Salt Stress, Vikas Belamkar, Nathan T. Weeks, Arvind K. Bharti, Andrew Farmer, Michelle A. Graham, Steven B. Cannon

Agronomy & Horticulture -- Faculty Publications

Background:

The homeodomain leucine zipper (HD-Zip) transcription factor family is one of the largest plant specific superfamilies, and includes genes with roles in modulation of plant growth and response to environmental stresses. Many HD-Zip genes are characterized in Arabidopsis (Arabidopsis thaliana), and members of the family are being investigated for abiotic stress responses in rice (Oryza sativa), maize (Zea mays), poplar (Populus trichocarpa) and cucumber (Cucmis sativus). Findings in these species suggest HD-Zip genes as high priority candidates for crop improvement.

Results:

In this study we have identified members of the HD-Zip gene family in soybean cv. ‘Williams 82’, and ...


The Trehalose Pathway In Maize: Conservation And Gene Regulation In Response To The Diurnal Cycle And Extended Darkness, Clémence Henry, Samuel W. Bledsoe, Allison Siekman, Alec Kollman, Brian M. Waters, Regina Feil, Mark Stitt, L. Mark Lagrimini Sep 2014

The Trehalose Pathway In Maize: Conservation And Gene Regulation In Response To The Diurnal Cycle And Extended Darkness, Clémence Henry, Samuel W. Bledsoe, Allison Siekman, Alec Kollman, Brian M. Waters, Regina Feil, Mark Stitt, L. Mark Lagrimini

Agronomy & Horticulture -- Faculty Publications

Energy resources in plants are managed in continuously changing environments, such as changes occurring during the day/night cycle. Shading is an environmental disruption that decreases photosynthesis, compromises energy status, and impacts on crop productivity. The trehalose pathway plays a central but not well-defined role in maintaining energy balance. Here, we characterized the maize trehalose pathway genes and deciphered the impacts of the diurnal cycle and disruption of the day/night cycle on trehalose pathway gene expression and sugar metabolism. The maize genome encodes 14 trehalose-6-phosphate synthase (TPS) genes, 11 trehalose-6-phosphate phosphatase (TPP) genes, and one trehalase gene. Transcript abundance ...


Msh1-Induced Non-Genetic Variation Provides A Source Of Phenotypic Diversity In Sorghum Bicolor, Roberto De La Rosa Santamaria, Mon-Ray Shao, Guomei Wang, David O. Nino-Liu, Hardik Kundariya, Yashitola Wamboldt, Ismail M. Dweikat, Sally Ann Mackenzie Sep 2014

Msh1-Induced Non-Genetic Variation Provides A Source Of Phenotypic Diversity In Sorghum Bicolor, Roberto De La Rosa Santamaria, Mon-Ray Shao, Guomei Wang, David O. Nino-Liu, Hardik Kundariya, Yashitola Wamboldt, Ismail M. Dweikat, Sally Ann Mackenzie

Agronomy & Horticulture -- Faculty Publications

MutS Homolog 1 (MSH1) encodes a plant-specific protein that functions in mitochondria and chloroplasts. We showed previously that disruption or suppression of the MSH1 gene results in a process of developmental reprogramming that is heritable and non-genetic in subsequent generations. In Arabidopsis, this developmental reprogramming process is accompanied by striking changes in gene expression of organellar and stress response genes. This developmentally reprogrammed state, when used in crossing, results in a range of variation for plant growth potential. Here we investigate the implications of MSH1 modulation in a crop species. We found that MSH1-mediated phenotypic variation in Sorghum bicolor ...


Cold Frames, High Tunnels, And Greenhouses: Choose A Growing Structure Best For You, Stacy A. Adams, Kim A. Todd Sep 2014

Cold Frames, High Tunnels, And Greenhouses: Choose A Growing Structure Best For You, Stacy A. Adams, Kim A. Todd

Agronomy & Horticulture -- Faculty Publications

Cold frames, high tunnels, and greenhouses are plant growth structures that can be used to extend the growing season or allow for use year around for growing high value specialty crops. This publication discusses the various attributes and applications of each so that plant producers in teaching, research, extension, commercially or as a hobby can determine the best structure to meet individual needs.

Cold frames, high tunnels, and greenhouses are structures that provide hobbyists and growers with options for plant protection and season extension. They vary in cost, size, structural complexity, and ability for year-round usage. The small cold frame ...


Contrasting Metabolism In Perenniating Structures Of Upland And Lowland Switchgrass Plants Late In The Growing Season, Nathan Palmer, Aaron J. Saathoff, Christian M. Tobias, Paul Twigg, Yuannan Xia, Kenneth P. Vogel, Soundararajan Madhavan, Scott E. Sattler, Gautam Sarath Aug 2014

Contrasting Metabolism In Perenniating Structures Of Upland And Lowland Switchgrass Plants Late In The Growing Season, Nathan Palmer, Aaron J. Saathoff, Christian M. Tobias, Paul Twigg, Yuannan Xia, Kenneth P. Vogel, Soundararajan Madhavan, Scott E. Sattler, Gautam Sarath

Agronomy & Horticulture -- Faculty Publications

Background: Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields is to move southern adapted lowland cultivars to more northern latitudes. However, many southerly adapted switchgrass germplasm can suffer significant winter kill in northerly climes.

Materials and Methods: Here, we have applied next-generation sequencing in combination with biochemical analyses to query the metabolism of crowns and rhizomes obtained from two contrasting switchgrass cultivars. Crowns and rhizomes from field-grown lowland (cv Kanlow) and upland (cv Summer) switchgrass cultivars were collected from three randomly selected post-flowering plants ...


Fall Seed Guide 2014, Teshome Regassa, P. Stephen Baenziger, Greg Kruger, Dipak Santra Aug 2014

Fall Seed Guide 2014, Teshome Regassa, P. Stephen Baenziger, Greg Kruger, Dipak Santra

Agronomy & Horticulture -- Faculty Publications

Crops include in this guide are winter wheat, winter barley, and triticale. You may receive this guide in the mail or through the University of Nebraska Extension network. The data and other information this guide is based on can be found at our web site: http://cropwatch.unl.edu/ varietytest/ and http://digitalcommons.unl.edu/ncia. Additional information is available at the wheat variety virtual tour web site http://cropwatch.unl.edu/wheat/virtual or the winter wheat variety selection tool page http://citnews.unl.edu/winter_wheat_tool/index.shtml.

Our rainfed plots in Keith County were lost to hail damage ...


Synthesis Of The Effect Of Fire On Lesser Prairie‐Chickens, Eric Thacker, Dirac L. Twidwell Jr Jun 2014

Synthesis Of The Effect Of Fire On Lesser Prairie‐Chickens, Eric Thacker, Dirac L. Twidwell Jr

Agronomy & Horticulture -- Faculty Publications

In this synthesis, we reviewed the scientific literature pertaining to the effects of fire on lesser prairie‐chickens and their habitat. Overall, research is generally lacking that directly assesses relationships among fire, both as a regime and a discrete event, to its effect on lesser prairie‐chicken behavior and habitat value. Yet, research has quantified the effects of fire on vegetation throughout the distribution of the lesser prairie‐chicken, which has been linked to known metrics of habitat value and broad landscape‐level habitat requirements.

To summarize the literature, lesser prairie‐chickens require broad landscapes of fire‐dependent grass‐shrub ...


Drivers Of Spatial And Temporal Variation In Soybean Yield And Irrigation Requirements In The Western Us Corn Belt, Patricio Grassini, Jessica A. Torrion, Kenneth Cassman, Haishun Yang, James Specht May 2014

Drivers Of Spatial And Temporal Variation In Soybean Yield And Irrigation Requirements In The Western Us Corn Belt, Patricio Grassini, Jessica A. Torrion, Kenneth Cassman, Haishun Yang, James Specht

Agronomy & Horticulture -- Faculty Publications

Both rainfed and irrigated soybean production are important in Nebraska (western US Corn Belt), accounting for a respective 48 and 52% of the state’s soybean production of 7 Mt on a respective 55 and 45% share of the state soybean area of 1.9 Mha. To date, no assessment of factors that may account for regional and inter-annual variation in yield and irrigation amount has been performed. To accomplish that objective, we evaluated a database containing on-farm field yields and total irrigation amount used in those fields. These data have been collected annually from ca. 1000 soybean fields in ...


Stacking Of A Stearoyl-Acp Thioesterase With A Dual-Silenced Palmitoyl-Acp Thioesterase And Δ12 Fatty Acid Desaturase In Transgenic Soybean, Hyunwoo Park, George L. Graef, Yixiang Xu, Patrick Tenopir, Thomas E. Clemente May 2014

Stacking Of A Stearoyl-Acp Thioesterase With A Dual-Silenced Palmitoyl-Acp Thioesterase And Δ12 Fatty Acid Desaturase In Transgenic Soybean, Hyunwoo Park, George L. Graef, Yixiang Xu, Patrick Tenopir, Thomas E. Clemente

Agronomy & Horticulture -- Faculty Publications

Soybean (Glycine max (L.) Merr) is valued for both its protein and oil, whose seed is composed of 40% and 20% of each component, respectively. Given its high percentage of polyunsaturated fatty acids, linoleic acid and linolenic acid, soybean oil oxidative stability is relatively poor. Historically food processors have employed a partial hydrogenation process to soybean oil as a means to improve both the oxidative stability and functionality in end-use applications. However, the hydrogenation process leads to the formation of trans-fats, which are associated with negative cardiovascular health. As a means to circumvent the need for the hydrogenation process, genetic ...


First Report Of Curvularia Inaequalis And Bipolaris Spicifera Causing Leaf Blight Of Buffalograss In Nebraska, B. S. Amaradasa, K. Amundsen Feb 2014

First Report Of Curvularia Inaequalis And Bipolaris Spicifera Causing Leaf Blight Of Buffalograss In Nebraska, B. S. Amaradasa, K. Amundsen

Agronomy & Horticulture -- Faculty Publications

During the summer of 2011, foliar blight was observed on buffalograss (Buchloë dactyloides (Nutt.) Engelm.) lawns in Lincoln and Waverly, Nebraska. Disease symptoms were common when buffalograss was growing above 30°C and in drought conditions. Disease symptoms began as dark brown oblong leaf spots, followed by leaf tip dieback and eventual blighting of entire tillers. Leaf infections would progress into patches of thinning turf. Diseased leaf pieces were rinsed in distilled water and placed on 1.5% water agar. Two mitosporic fungal species having conidial morphology of Curvularia and Bipolaris were isolated. Colonies of Curvularia isolates grown on ¼ ...


The Interaction Of Climate Change, Land Cover, And Political Representation In The Usa, Brady W. Allred, Dirac L. Twidwell, Samuel D. Fuhlendorf Jan 2014

The Interaction Of Climate Change, Land Cover, And Political Representation In The Usa, Brady W. Allred, Dirac L. Twidwell, Samuel D. Fuhlendorf

Agronomy & Horticulture -- Faculty Publications

The difficulties in tackling climate change are inherently complex and primarily centered on political and social values. This is evident in the United States where political divisions and polarizations are fundamental barriers to advancing national policies, which in turn hinder international agreements, mitigation, and adaptation. Within the United States, the vast majority of agricultural and natural resource lands are projected to incur significant climate departures and are represented by the Republican Party. The resources and economic sectors that will be directly affected by climate change are represented by national leadership that is unlikely to accept policies to prevent or adapt ...


Quantitative Trait Locus Analysis Of Unsaturated Fatty Acids In A Recombinant Inbred Population Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan Jan 2014

Quantitative Trait Locus Analysis Of Unsaturated Fatty Acids In A Recombinant Inbred Population Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan

Agronomy & Horticulture -- Faculty Publications

Soybean [Glycine max (L.) Merr.] is an important oilseed crop which produces about 30 %of the world’s edible vegetable oil. The quality of soybean oil is determined by its fatty acid composition. Soybean oil high in oleic and low in linolenic fatty acids is desirable for human consumption and other uses. The objectives of this study were to identify quantitative trait loci (QTLs) for unsaturated fatty acids and to evaluate the genetic effects of single QTL and QTL combinations in soybean. A population of recombinant inbred lines derived from the cross of SD02-4-59 X A02-381100 was evaluated for fatty ...


Porocercospora Seminalis Gen. Et Comb. Nov., The Causal Organism Of Buffalograss False Smut, Bimal S. Amaradasa, Hugo Madrid, Johannes Z. Groenewald, Pedro W. Crous, Keenan L. Amundsen Jan 2014

Porocercospora Seminalis Gen. Et Comb. Nov., The Causal Organism Of Buffalograss False Smut, Bimal S. Amaradasa, Hugo Madrid, Johannes Z. Groenewald, Pedro W. Crous, Keenan L. Amundsen

Agronomy & Horticulture -- Faculty Publications

False smut caused by Cercospora seminalis is an important disease of buffalograss (Buchloe dactyloides) affecting seed production. The pathogen prevents normal caryopsis development and causes considerable yield loss and reduced seed germination. The current taxonomic placement of the false-smut causal pathogen in the genus Cercospora is incorrect based on its morphological characteristics and DNA phylogeny. In the present study the phylogenetic position of C. seminalis is clarified based on DNA sequence analysis of three loci namely the internal transcribed spacer (ITS) region, partial nuclear ribosomal large subunit (LSU) and partial sequences of the RNA polymerase II second largest subunit (RPB2 ...


Dt2 Is A Gain-Of-Function Mads-Domain Factor Gene That Specifies Semideterminacy In Soybean, Jieqing Ping, Yunfeng Liu, Lianjun Sun, Meixia Zhao, Yinghui Li, Zongxiang Tang, Hanh Nguyen, Zhixi Tian, Lijuan Qiu, Randall L. Nelson, Thomas E. Clemente, James Specht, Jianxin Ma Jan 2014

Dt2 Is A Gain-Of-Function Mads-Domain Factor Gene That Specifies Semideterminacy In Soybean, Jieqing Ping, Yunfeng Liu, Lianjun Sun, Meixia Zhao, Yinghui Li, Zongxiang Tang, Hanh Nguyen, Zhixi Tian, Lijuan Qiu, Randall L. Nelson, Thomas E. Clemente, James Specht, Jianxin Ma

Agronomy & Horticulture -- Faculty Publications

Similar to Arabidopsis thaliana, the wild soybeans (Glycine soja) and many cultivars exhibit indeterminate stem growth specified by the shoot identity gene Dt1, the functional counterpart of Arabidopsis TERMINAL FLOWER1 (TFL1). Mutations in TFL1 and Dt1 both result in the shoot apical meristem (SAM) switching from vegetative to reproductive state to initiate terminal flowering and thus produce determinate stems. A second soybean gene (Dt2) regulating stem growth was identified, which, in the presence of Dt1, produces semideterminate plants with terminal racemes similar to those observed in determinate plants. Here, we report positional cloning and characterization of Dt2, a dominant MADS ...


Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang Jan 2014

Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang

Agronomy & Horticulture -- Faculty Publications

No abstract provided.


The Carbon-Nitrogen Balance Of The Nodule And Its Regulation Under Elevated Carbon Dioxide Concentration, Marc Libault Jan 2014

The Carbon-Nitrogen Balance Of The Nodule And Its Regulation Under Elevated Carbon Dioxide Concentration, Marc Libault

Agronomy & Horticulture -- Faculty Publications

Legumes have developed a unique way to interact with bacteria: in addition to preventing infection from pathogenic bacteria like any other plant, legumes also developed a mutualistic symbiotic relationship with one gender of soil bacteria: rhizobium. This interaction leads to the development of a new root organ, the nodule, where the differentiated bacteria fix for the plant the atmospheric dinitrogen (atmN2). In exchange, the symbiont will benefit from a permanent source of carbon compounds, products of the photosynthesis. The substantial amounts of fixed carbon dioxide dedicated to the symbiont imposed to the plant a tight regulation of the nodulation ...


A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson Jan 2014

A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson

Agronomy & Horticulture -- Faculty Publications

Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and has a role in sustainable agriculture owing to its ability to fix atmospheric nitrogen. We assembled 473 Mb of the 587-Mb genome and genetically anchored 98% of this sequence in 11 chromosome-scale pseudomolecules. We compared the genome for the common bean against the soybean genome to find changes in soybean resulting from polyploidy. Using resequencing of 60 wild individuals and 100 landraces from the genetically differentiated Mesoamerican and Andean gene pools, we confirmed 2 independent domestications from genetic pools that diverged before human colonization. Less ...


Eqtl Networks Reveal Complex Genetic Architecture In The Immature Soybean Seed, Yung-Tsi Bolon, D. Hyten, James H. Orf, Carroll P. Vance, Gary J. Muehlbauer Jan 2014

Eqtl Networks Reveal Complex Genetic Architecture In The Immature Soybean Seed, Yung-Tsi Bolon, D. Hyten, James H. Orf, Carroll P. Vance, Gary J. Muehlbauer

Agronomy & Horticulture -- Faculty Publications

The complex network of regulatory factors and interactions involved in transcriptional regulation within the seed is not well understood. To evaluate gene expression regulation in the immature seed, we utilized a genetical genomics approach on a soybean [Glycine max (L.) Merr.] recombinant inbred line (RIL) population and produced a genome-wide expression quantitative trait loci (eQTL) dataset. The validity of the dataset was confirmed by mapping the eQTL hotspot for flavonoid biosynthesis-related genes to a region containing repeats of chalcone synthase (CHS) genes known to correspond to the soybean inhibitor locus that regulates seed color. We then identified eQTL for genes ...


Nebraska’S Groundwater Legacy: Nitrate Contamination Beneath Irrigated Cropland, Mary E. Exner Spaulding, Aaron J. Hirsh, Roy F. Spalding Jan 2014

Nebraska’S Groundwater Legacy: Nitrate Contamination Beneath Irrigated Cropland, Mary E. Exner Spaulding, Aaron J. Hirsh, Roy F. Spalding

Agronomy & Horticulture -- Faculty Publications

A 31 year record of ~44,000 nitrate analyses in ~11,500 irrigation wells was utilized to depict the decadal expansion of groundwater nitrate contamination (N>10 mg/L) in the irrigated corn-growing areas of eastern and central Nebraska and analyze long-term nitrate concentration trends in 17 management areas (MAs) subject to N fertilizer and budgeting requirements. The 1.3 M contaminated hectares were characterized by irrigation method, soil drainage, and vadose zone thickness and lithology. The areal extent and growth of contaminated groundwater in two predominately sprinkler-irrigated areas was only ~20% smaller beneath well-drained silt loams with thick clayey-silt ...


Multilocation Corn Stover Harvest Effects On Crop Yields And Nutrient Removal, Douglas L. Karlen, Stuart J. Birrell, Jane M.F. Johnson, Shannon L. Osborne, Thomas E. Schumacher, Gary E. Varvel, Richard B. Ferguson, Jeff M. Novak, James R. Frederick, John M. Baker, John A. Lamb, Paul R. Alder, Greg W. Roth, Emerson D. Nafziger Jan 2014

Multilocation Corn Stover Harvest Effects On Crop Yields And Nutrient Removal, Douglas L. Karlen, Stuart J. Birrell, Jane M.F. Johnson, Shannon L. Osborne, Thomas E. Schumacher, Gary E. Varvel, Richard B. Ferguson, Jeff M. Novak, James R. Frederick, John M. Baker, John A. Lamb, Paul R. Alder, Greg W. Roth, Emerson D. Nafziger

Agronomy & Horticulture -- Faculty Publications

Corn (Zea mays L.) stover was identified as an important feedstock for cellulosic bioenergy production because of the extensive area upon which the crop is already grown. This report summarizes 239 site-years of field research examining effects of zero, moderate, and high stover removal rates at 36 sites in seven different states. Grain and stover yields from all sites as well as N, P, and K removal from 28 sites are summarized for nine longitude and six latitude bands, two tillage practices (conventional vs no tillage), two stoverharvest methods (machine vs calculated), and two crop rotations {continuous corn (maize) vs ...


Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat Jan 2014

Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat

Agronomy & Horticulture -- Faculty Publications

Background: Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having differential response to low-N stress.

Results: Illumina RNA-sequencing detected several common differentially expressed genes (DEGs) between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three sensitive genotypes ...


A Genome-Wide Association Study Of Seed Protein And Oil Content In Soybean, Eun-Young Hwang, Qijian Song, Gaofeng Jia, James E. Specht, David L. Hyten, Jose Costa, Perry B. Cregan Jan 2014

A Genome-Wide Association Study Of Seed Protein And Oil Content In Soybean, Eun-Young Hwang, Qijian Song, Gaofeng Jia, James E. Specht, David L. Hyten, Jose Costa, Perry B. Cregan

Agronomy & Horticulture -- Faculty Publications

Background: Association analysis is an alternative to conventional family-based methods to detect the location of gene(s) or quantitative trait loci (QTL) and provides relatively high resolution in terms of defining the genome position of a gene or QTL. Seed protein and oil concentration are quantitative traits which are determined by the interaction among many genes with small to moderate genetic effects and their interaction with the environment. In this study, a genome-wide association study (GWAS) was performed to identify quantitative trait loci (QTL) controlling seed protein and oil concentration in 298 soybean germplasm accessions exhibiting a wide range of ...


How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha Jan 2014

How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha

Agronomy & Horticulture -- Faculty Publications

Potential consequences of climate change on crop production can be studied using mechanistic crop simulation models. While a broad variety of maize simulation models exist, it is not known whether different models diverge on grain yield responses to changes in climatic factors, or whether they agree in their general trends related to phenology, growth, and yield. With the goal of analyzing the sensitivity of simulated yields to changes in temperature and atmospheric carbon dioxide concentrations [CO2], we present the largest maize crop model intercomparison to date, including 23 different models. These models were evaluated for four locations representing a wide ...


Global Changes In Mineral Transporters In Tetraploid Switchgrasses (Panicum Virgatum L.), Nathan A. Palmer, Aaron J. Saathoff, Brian M. Waters, Teresa Donze, Tiffany M. Heng-Moss, Paul Twigg, Christian M. Tobias, Gautam Sarath Jan 2014

Global Changes In Mineral Transporters In Tetraploid Switchgrasses (Panicum Virgatum L.), Nathan A. Palmer, Aaron J. Saathoff, Brian M. Waters, Teresa Donze, Tiffany M. Heng-Moss, Paul Twigg, Christian M. Tobias, Gautam Sarath

Agronomy & Horticulture -- Faculty Publications

Switchgrass (Panicum virgatum L) is a perennial, C4 grass with great potential as a biofuel crop. An in-depth understanding of the mechanisms that control mineral uptake, distribution, and remobilization will benefit sustainable production. Nutrients are mobilized from aerial portions to below-ground crowns and rhizomes as a natural accompaniment to above-ground senescence post seed-set. Mineral uptake and remobilization is dependent on transporters, however, little if any information is available about the specific transporters that are needed and how their relative expression changes over a growing season.Using well-defined classes of mineral transporters, we identified 520 genes belonging to 40 different transporter ...


Switchgrass (Panicum Virgatum L) Flag Leaf Transcriptomes Reveal Molecular Signatures Of Leaf Development, Senescence, And Mineral Dynamics, Nathan A. Palmer, Teresa Donze-Reiner, David Horvath, Tiffany Heng-Moss, Brian M. Waters, Christian M. Tobias, Gautam Sarath Jan 2014

Switchgrass (Panicum Virgatum L) Flag Leaf Transcriptomes Reveal Molecular Signatures Of Leaf Development, Senescence, And Mineral Dynamics, Nathan A. Palmer, Teresa Donze-Reiner, David Horvath, Tiffany Heng-Moss, Brian M. Waters, Christian M. Tobias, Gautam Sarath

Agronomy & Horticulture -- Faculty Publications

Switchgrass flag leaves can be expected to be a source of carbon to the plant, and its senescence is likely to impact the remobilization of nutrients from the shoots to the rhizomes. However, many genes have not been assigned a function in specific stages of leaf development. Here, we characterized gene expression in flag leaves over their development. By merging changes in leaf chlorophyll and the expression of genes for chlorophyll biosynthesis and degradation, a four-phase molecular roadmap for switchgrass flag leaf ontogeny was developed. Genes associated with early leaf development were up-regulated in phase 1. Phase 2 leaves had ...


Comparison Of Winter Strawberry Production In A Commercial Heated High Tunnel Versus A University Greenhouse, Ellen T. Paparozzi, Ryan Pekarek, George E. Meyer, Stacy A. Adams, M. Elizabeth Conley, David P. Lambe, Paul Read, Erin E. Blankenship Jan 2014

Comparison Of Winter Strawberry Production In A Commercial Heated High Tunnel Versus A University Greenhouse, Ellen T. Paparozzi, Ryan Pekarek, George E. Meyer, Stacy A. Adams, M. Elizabeth Conley, David P. Lambe, Paul Read, Erin E. Blankenship

Agronomy & Horticulture -- Faculty Publications

For the past 4 years, the University of Nebraska strawberry team has worked to develop low cost, sustainable methods for farmers and growers to produce strawberries in a double polyethylene greenhouse during the winter. This past year, this growing system was adapted to become a commercial grower’s heated high tunnel for the winter/spring of 2013-14. The idea was to scale up to a farm-size demonstration and compare it to the university greenhouse production system with a goal to expand marketing opportunities for strawberries into the winter season.


Greenhouse Gas Emissions From Soil Treated With Dung Pats, Kenneth Evans, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Pamela Sutton, Kent M. Eskridge Jan 2014

Greenhouse Gas Emissions From Soil Treated With Dung Pats, Kenneth Evans, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Pamela Sutton, Kent M. Eskridge

Agronomy & Horticulture -- Faculty Publications

No abstract provided.