Life Sciences Commons^™

Integrating Human Omics Data To Prioritize Candidate Genes., Yong Chen, Xuebing Wu, Rui Jiang

Faculty Scholarship for the College of Science & Mathematics

BACKGROUND: The identification of genes involved in human complex diseases remains a great challenge in computational systems biology. Although methods have been developed to use disease phenotypic similarities with a protein-protein interaction network for the prioritization of candidate genes, other valuable omics data sources have been largely overlooked in these methods.

METHODS: With this understanding, we proposed a method called BRIDGE to prioritize candidate genes by integrating disease phenotypic similarities with such omics data as protein-protein interactions, gene sequence similarities, gene expression patterns, gene ontology annotations, and gene pathway memberships. BRIDGE utilizes a multiple regression model with lasso penalty to …

Racial Differences In Self-Efficacy Expectations For Exercise, Selena P. Smith

Honors Theses

This project examined the link between race and self-efficacy. Self-efficacy pertains to an individual’s confidence in ability to complete or engage in a particular task or activity (Bandura, 1986). Prior research shows that self-efficacy has a great influence on exercise initiation and adherence (Bandura, 1986; Dishman, 1982; Rodgers and Sullivan, 2001). Research also supports that Caucasians are more likely to participate in exercise than African Americans (Centers for Disease Control and Prevention, 2000).

Participants included 51 females, 31 Caucasians and 20 African Americans, between the ages of 18 and 50 years old. The women were recruited from local fitness gyms, …

Table For One, Food For Two: A Socio-Economic Perspective On American Obesity, Celestina Victoria Brunetti

Honors Theses - Providence Campus

Research indicates that obesity is linked to socio-economic factors and genetics. Both have an impact on whether a person is or becomes obese. The paper focuses on the impact of the change in food supply along with socio-economic factors, specifically access, time, and cost, and how these factors have affected the American obesity epidemic. This was assessed in part through a case study of the Smith Hill area of Providence, Rhode Island, a food desert that is comprised mainly of Americans near the poverty line, and the Wellspring La Jolla camp—a camp for overweight children from upper-middle class families. The …

The Relationship Between Television Viewing Time And Cardiorespiratory Fitness In Adult Women, Peter Jacob Arens

Theses and Dissertations

Purpose: The present investigation was conducted to assess the relationship between television viewing time and cardiorespiratory fitness. Design: Cross-sectional. Setting: Intermountain West. Participants: 302 middle-aged women. Method: TV viewing was assessed using a questionnaire. Cardiorespiratory fitness was measured using a maximal graded treadmill test. Physical activity (PA) was evaluated using Actigraph accelerometers worn over seven consecutive days, while body fat percentage (BF%) was measured using air displacement plethysmography (Bod Pod). Results: (Mean ± SD) age: 40.2 ± 3.0 years. VO2max of the frequent (≥ 3hrs/day) TV group (32.6 ± 6.4) was significantly lower than both the moderate (1-2 hrs/day) (36.2 …

Aspirin And Low-Molecular Weight Heparin Combination Therapy Effectively Prevents Recurrent Miscarriage In Hyperhomocysteinemic Women., Pratip Chakraborty, Sayani Banerjee, Piyali Saha, Shyam Sundar Nandi, Sunita Sharma, Sourendra K. Goswami, Baidyanath Chakravarty, Syed N. Kabir

Journal Articles: Cellular & Integrative Physiology

The management of recurrent pregnancy loss (RPL) still remains a great challenge, and women with polycystic ovarian syndrome (PCOS) are at a greater risk for spontaneous abortion. Treatment with low-molecular-weight heparin (LMWH) has become an accepted treatment option for women with RPL; however, the subgroup of women, who are likely to respond to LMWH, has not been precisely identified. The present study evaluated the efficacy of LMWH with reference to PCOS and associated metabolic phenotypes including hyperhomocysteinemia (HHcy), insulin resistance (IR) and obesity. This prospective observational study was conducted at Institute of Reproductive Medicine, Kolkata, India. A total of 967 …

Metformin-Induced Pedf Expression Regulates Cell Proliferation And Lipid Metabolism In Prostate Cancer Cells, Miguel Angel Tolentino

Theses and Dissertations

Prostate cancer (PCa) is one of the most common cancers in American males. A high fat diet and obesity accelerate PCa progression and increase the 1risk of death from disease. Epidemiological studies have indicated that PCa patients with type 2 diabetes have higher mortality rates than PCa patients without diabetes. Type 2 diabetics who are on metformin, a drug to control blood sugar levels, show a delay in PCa progression in comparison with PCa patients with type 2 diabetes who are not on metformin. It has been proposed that metformin inhibits proliferation via activation of AMP-activated protein kinase (AMPK) or …

Aging And Obesity Models In Osteoarthritis, Sara Ohora

Electronic Thesis and Dissertation Repository

Osteoarthritis (OA) is a degenerative joint disease whose exact mechanisms are not well understood, but aging and obesity are common risk factors. To determine the role of transforming growth factor-alpha (TGFα) in obesity-associated OA, Tgfa null and control mice on control and high fat diets were compared. In addition, spontaneous OA was investigated in aging C57BL/6J mice. Quantitative magnetic resonance was used to assess body composition in the obesity model, while gait analysis, histological staining and OARSI scoring were used to determine OA in both models. Mice on high fat diet developed no OA, but Tgfa null mice weighed significantly …

The Obesity Epidemic, Lenka Kollar, Evienne Epifano, Molly Mckneight, Jeff Miskovich, Heather Moore

Student Papers in Public Policy

The incidence of chronic, noncommunicable diseases, such as heart disease, is increasing at an alarming rate on the global scale. The growing prevalence of overweight and obesity have led to an upsurge in cases of diabetes and other obesity-related diseases. About 18 million people die every year from heart disease, of which diabetes and obesity are major predisposing factors. Worldwide, more than 1.1 billion adults are overweight, 312 million of which are obese. The number of children that are overweight or obese is also growing (Hossain, Parvez et al.). Obesity, and the associated diseases, has become a worldwide epidemic and …

Maternal Acculturation Levels And Their Effect On Children's Food Intake, Maternal Perceptions Of Body Size And Maternal Stress, Tracie A. Chavoor

Master's Theses

In the U.S., obesity has hit alarming rates and affects Hispanic children disproportionately. Acculturation, the shift from one culture’s values, beliefs and practices to those of a new culture, may have both negative and positive health effects on immigrants. The objectives of this study were to determine the association between the level of acculturation (low acculturation or high acculturation) of low-income Latina mothers and the foods consumed by their children (0 to 36 months old). The association between acculturation and maternal perceptions of infant and toddler body size, maternal perceptions of her own body size and maternal parenting stress were …

Preventing Paediatric Obesity; Recommendations From A Community-Based Qualitative Investigation, Patricia Tucker, Jennifer Irwin, L. Sangster Bouck, Meizi He, G. Pollett

Trish Tucker

Childhood obesity is on the rise, and interventions targeted at pre-school-aged children are essential for the primary prevention of this disease. Physical activity programming and decreasing screen viewing may be advantageous ways to reduce the early onset of obesity. Parents' perceptions regarding effective tools and programmes to target obesity are needed to develop an efficacious programme. Ten semi-structured focus groups were conducted for this qualitative study. Two experienced moderators facilitated all focus groups which were conducted in local day cares, workplaces and play groups, frequented by parents. All focus groups were audio-recorded and transcribed verbatim. Strategies to ensure trustworthiness of …

Molecular Characterization Of A Patient Presumed To Have Prader-Willi Syndrome, Marina Falaleeva, Carlos R. Sulsona, Horst R. Zielke, Kathleen M. Currey, Pierre De La Grange, Vahid Aslanzadeh, Daniel J. Driscoll, Stefan Stamm

Molecular and Cellular Biochemistry Faculty Publications

Prader-Willi syndrome (PWS) is caused by the loss of RNA expression from an imprinted region on chromosome 15 that includes SNRPN, SNORD115, and SNORD116. Currently, there are no mouse models that faithfully reflect the human phenotype and investigations rely on human post-mortem material. During molecular characterization of tissue deposited in a public brain bank from a patient diagnosed with Prader-Willi syndrome, we found RNA expression from SNRPN, SNORD115, and SNORD116 which does not support a genetic diagnosis of Prader-Willi syndrome. The patient was a female, Caucasian nursing home resident with history of morbid obesity (BMI 56.3) and mental retardation. She …

Acceleration Of The Panin Development In Mice Expressing Oncogenic K-Ras Due To A High Fat Diet, Bincy Philip

Dissertations & Theses (Open Access)

Obesity is postulated to be one of the major risk factors for pancreatic cancer, and recently it was indicated that an elevated body mass index (BMI correlates strongly with a decrease in patient survival. Despite the evident relationship, the molecular mechanisms involved are unclear. Oncogenic mutation of K-Ras is found early and is universal in pancreatic cancer. Extensive evidence indicates oncogenic K-Ras is not entirely active and it requires a triggering event to surpass the activity of Ras beyond the threshold necessary for a Ras-inflammation feed-forward loop. We hypothesize that high fat intake induces a persistent low level inflammatory response …

Diet-Induced Obesity Decreases Liver Iron Stores In Mice Fed Iron Deficient, Adequate, Or Excessive Diets, Brett J. Healy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Epidemiological and observational evidence suggests that obesity is related to poor Fe status. To determine interactions between obesity, dietary Fe intake and Fe status; male, weanling C57BL/6J mice were fed either high fat diets to induce obesity or a standard diet for 16 weeks. Fe concentrations of both the high fat or control diet (4.5 vs 3.8 kcal/g) were set at: 5, 50 or 500 mg Fe/kg diet. Mice fed the high fat diets had significantly higher percentage body fat (17.9%) compared to mice fed control diets (5.3%, P<0.001). Among obese mice, dietary Fe levels did not significantly influence body composition. Conversely among lean mice, mice fed the iron excessive diet had significantly less fat mass when compared to mice fed the iron deficient diet (P<0.05). Obesity and/or dietary Fe concentration did not significantly affect plasma Fe levels. ANOVA analysis showed significant effects of diet-induced obesity, dietary Fe and an interaction between both factors on liver Fe levels (P< 0.05). Obese mice had significantly lowered liver Fe levels compared to lean cohorts fed the same amount of dietary Fe (P<0.05 for all comparisons). Moreover, lean mice fed the Fe deficient diet (5 mg Fe/kg diet) had similar liver Fe levels (127 mg Fe/kg ± 0.04) compared to obese mice fed the 50 mg Fe/kg diet (132 mg Fe/kg ± 0.05). These data suggest that obesity, independent of dietary Fe intake, influences liver Fe stores.

The Relationship Between Calcium Intake And Body Mass Index In The Participants Of Hub City Steps, Laura P. Ewoldt

Honors Theses

Background: The social, economic, and health impacts of unhealthy dietary behaviors and sedentary lifestyles are quite significant and even more so in the rural South. The majority of individuals in the US do not adhere to the Dietary Guidelines for Americans, specifically fruits, vegetables, and calcium.

Purpose: The purpose of this study was to address the issue of calcium intake and obesity in a population of primarily African American women in south Mississippi and to determine if levels of calcium intake had a relationship with body mass index.

Methods and Analysis: Data for the current study was …

Assessment Of Dietary Behaviors Of College Students Participating In The Health Promotion Program Bucs: Live Well, Courtney E. Mckinney

Electronic Theses and Dissertations

Rates of adult obesity in the United States continue to rise, especially among the college-age population who typically practice poor dietary choices, contributing to weight gain. College-age students experience a transitional phase called ‘emerging adulthood’ where they learn autonomy in many areas that influence long-term health status. The purpose of the online health promotion program BUCS: Live Well was to promote self-efficacy in ability to incorporate positive dietary changes, assess incidences of positive dietary habits after completing the program, and determine if weight status, residence, and health perceptions influence dietary behaviors. BUCS: Live Well was successful in assessing current dietary …

Elongation Factor 1a-1 And Hepatocyte Response To Fatty Acid Excess, Alexandra M. Stoianov

Electronic Thesis and Dissertation Repository

Obesity is associated with elevated levels of serum fatty acids, which accumulate in nonadipose tissues including the liver. Elongation factor 1A-1 (EF1A-1) has previously been shown to participate in the cell stress and death response of cardiomyocytes to excess saturated fatty exposure, and in steatotic mouse myocardium. In this thesis, the hypothesis that the hepatocyte response to fatty acid overload involves EF1A-1 was tested. EF1A-1 expression was induced in the livers of obese mice in association with severe hepatic steatosis, and in HepG2 human hepatoma cells in response to excess palmitate. Partial translocation of EF1A-1 from the ER to polymerized …

Validation Of Omron™ Pedometers Using Mti Accelerometers For Use With Children, Bradley P. Peters, Kate A. Heelan Phd, Bryce M. Abbey

International Journal of Exercise Science

International Journal of Exercise Science 6(2) : 106-113, 2013. The MTI accelerometer is highly regarded as a reliable means to measure physical activity in children (Trost et. al., 1998); however, it is not always a practical instrument to use. Pedometers offer an alternative method of activity measurement and are often more practical. PURPOSE: To validate Omron™ pedometer (steps/day) against MTI accelerometer (counts /day and moderate to vigorous physical activity (MVPA) min/day) for children. METHODS: 190 children (88 males, 102 females, 8.7±2.1 yrs, 76.9±27.5 BMI %tile) wore an MTI accelerometer and Omron™ pedometer attached to the same belt for 3.9±2.2 days. …

Myths, Presumptions, And Facts About Obesity, Krista Casazza, Kevin R. Fontaine, Arne Astrup, Leann L. Birch, Andrew W. Brown, Michelle M. Bohan Brown, Nefertiti Durant, Gareth Dutton, E. Michael Foster, Steven B. Heymsfield, Kerry Mciver, Tapan Mehta, Nir Menachemi, P.K. Newby, Russell R. Pate, Barbara J. Rolls, Bisakha Sen, Daniel L. Smith Jr, Diana M. Thomas, David B. Allison

Faculty Publications

BACKGROUND Many beliefs about obesity persist in the absence of supporting scientific evidence (presumptions); some persist despite contradicting evidence (myths). The promulgation of unsupported beliefs may yield poorly informed policy decisions, inaccurate clinical and public health recommendations, and an unproductive allocation of research resources and may divert attention away from useful, evidence-based information.

METHODS Using Internet searches of popular media and scientific literature, we identified, reviewed, and classified obesity-related myths and presumptions. We also examined facts that are well supported by evidence, with an emphasis on those that have practical implications for public health, policy, or clinical recommendations.

RESULTS We …

Co-Active Coaching As An Intervention For Obesity Among Female University Students, Melissa Van Zandvoort, Jennifer Irwin, Don Morrow

Donald Morrow

Purpose: The purpose of this study was to assess the impact of Co-active coaching on obese female university students’ body mass index (BMI), waist circumference (WC), functional health status and self-esteem, and conjointly to identify the coaching skills and primary agenda topics that facilitate coaching’s efficacy as an intervention for obesity.

Design: A multiple-baseline, single-subject research design was utilized.Methods: Two certified coaches provided an average of nine, 35-minute, one-on-one sessions with five students whose BMI?30kg/m2 (obesity threshold). Measures included BMI, WC, the Short-Form 36 (SF-36) Health Survey, and the Rosenberg Self-Esteem Scale. Visual inspection was used to analyze changes in …

The Impact Of Co-Active Life Coaching On Female University Students With Obesity, Melissa Van Zandvoort, Jennifer Irwin, Don Morrow

Donald Morrow

The purpose of this qualitative study was to explore the impact of Co-active life coaching on obese female university students. Five obese (BMI ≥ 30kg/m2), female university students received an average of nine weekly, 35-minute, one-on-one sessions with a certified coach. Semi- structured, in-depth interviews before and after participating in the coaching intervention were conducted, and inductive content analysis was utilized. Strategies to enhance data trustworthiness were incorporated throughout. Participants initially reported: struggling with barriers and experiencing pressure from family to lose weight; negative relationships with themselves; feeling self-conscious and remorse for their size and lifestyle choices. At the conclusion …

Participants' Perceived Utility Of Motivational Interviewing Using Co-Active Life Coaching Skills On Their Struggle With Obesity, Courtney Newnham-Kanas, Don Morrow, Jennifer Irwin

Donald Morrow

The purpose of this qualitative study was to investigate the impact of Motivational Interviewing (MI) using Co-Active Life Coaching skills as a treatment for adults (age, 35–55 years) struggling with obesity. Eight women (BMI≥30) who reside in London, Ontario, received 18 coaching sessions over six months with a Certified Professional Co-Active (CPCC) coach. Participants engaged in semi-structured pre- and post-interviews, along with a focus group six months after the last coaching session. The study's CPCC was also interviewed post-intervention to gain an understanding of what it was like coaching this population. All interviews were analysed using inductive content analysis. Following …

The Quantitative Assessment Of Motivational Interviewing Using Co-Active Life Coaching Skills As A Treatment For Adults Struggling With Obesity, Courtney Newnham-Kanas, Jennifer Irwin, Don Morrow, Danielle Battram

Donald Morrow

No abstract provided.

Microrna Function In Human Diseases, Sathish Kumar Natarajan, Mary Anne Smith, Cody J. Wehrkamp, Ashley M. Mohr, Justin L. Mott

Department of Biochemistry: Faculty Publications

MicroRNAs are emerging as a hot topic in research, and rightfully so. They show great promise as targets of treatment and as markers for common human diseases, such as cancer and metabolic diseases. In this review, we address some of the basic questions regarding micro- RNA function in human disease and the clinical significance of microRNAs. Specifically, micro- RNAs in epigenetics, cancer, and metabolic diseases are discussed, with examples taken from cholangiocarcinoma and nonalcoholic fatty liver disease.

Accounting For Product Substitution In The Analysis Of Food Taxes Targeting Obesity, Zhen Miao, John C. Beghin, Helen H. Jensen

Department of Agricultural Economics: Faculty Publications

We extend the existing literature on food taxes targeting obesity. We systematically incorporate the implicit substitution between added sugars and solid fats into a comprehensive food demand system and evaluate the effect of taxes on sugars and fats. The approach conditions how food and obesity taxes affect total calorie intake. The proposed methodology accounts for the ability of consumers to substitute leaner low-fat and low-sugar items for rich food items within the same food group. This substitution is integrated into a calibrated demand system in addition to the substitution among food groups, using recent food intake data and existing demand …

Metabolic Impact Of Sex Chromosomes, Jenny Link, Xuqi Chen, Arthur P. Arnold, Karen Reue

Biology

Obesity and associated metabolic diseases are sexually dimorphic. To provide better diagnosis and treatment for both sexes, it is of interest to identify the factors that underlie male/female differences in obesity. Traditionally, sexual dimorphism has been attributed to effects of gonadal hormones, which influence numerous metabolic processes. However, the XX/XY sex chromosome complement is an additional factor that may play a role. Recent data using the four core genotypes mouse model have revealed that sex chromosome complement—independently from gonadal sex—plays a role in adiposity, feeding behavior, fatty liver and glucose homeostasis. Potential mechanisms for the effects of sex chromosome complement …

Cardiorespiratory Responses In Healthy-Weight And Obese Women And Children, Elizabeth Ann Easley

Theses and Dissertations--Kinesiology and Health Promotion

A criterion method to evaluate cardiorespiratory health is measuring peak oxygen consumption (VO₂ peak) from a maximal graded exercise test (GXT). While VO₂ peak is a valuable measure, heart rate recovery (HRRec) and endothelial function (EF) also describe cardiorespiratory health and fitness. The purpose of this study was to investigate whether differences exist in VO₂ peak, HRRec, and EF between healthy-weight (HW) and obese (OB) women and children and to determine if there were significant correlations among these variables. A total of 60 women and children participated in this study. Anthropometric, body composition, resting heart rate and …

Fatty Acid Fate In Determining Oxidation And Inflammation In Adipose Tissue, Emilio Patrick Mottillo

Wayne State University Dissertations

Adipose tissue (AT) is a critical regulator of energy balance through its ability to store or oxidize free fatty acids (FFAs). White adipose tissue (WAT) functions as an anabolic organ to sequester and release FAs, in contrast brown adipose tissue (BAT) is a catabolic organ that oxidizes FAs. However, a comprehensive understanding of the role that FFAs play in the function of WAT and BAT is needed. Here we demonstrate that intracellular FAs enhance the expression of inflammatory cytokines by β3-AR activation in adipocytes, in which the expression of PAI-1 is partly mediated by the de novo synthesis of ceramides/sphingolipids. …

Linking Environmental Toxicant Exposure To Diabetes Susceptibility, Jannifer Beth Tyrrell

Wayne State University Dissertations

An important and unresolved question in the environmental health field is whether exposure to common environmental toxicants, such as dioxin and heavy metals like Pb, increase the risk of developing diabetes, especially in combination with other common metabolic stressors such as obesity.

Previous studies suggested that dioxin exposure increased peripheral insulin resistance but did not appear to cause fasting hyperglycemia or elevated hepatic glucose output. In concordance with those findings we observed that dioxin treatment caused a strong suppression of the expression of the key hepatic gluconeogenic genes PEPCK and G6Pase. However, this suppression was not solely mediated by the …

The Effect Of Alpha-Cyclodextrin On Acute Blood Lipid And Glycemic Responses To A Fat Containg Meal, Evan Neil Fletcher

Wayne State University Theses

Obesity and hyperlipidemia have become major concerns in the United States over the past 30 years. Alpha-Cyclodextrin (á-CD), a naturally occurring soluble dietary fiber, has been shown to reduce dietary fat absorption and improve blood lipid levels in an animal model (mouse and rat) and in human studies. In the current double blind study, 34 healthy male and female participants were recruited to test if á-CD had any acute effect on blood lipid and glycemic responses to a fat containing meal. The participants received the á-CD on one occasion and a placebo the other to determine if there was any …

Executing And Teaching Science--The Breast Cancer Genetics And Technology-Rich Curriculum Professional Development Studies Of A Science Educator, Regina Evarn Wragg

Theses and Dissertations

This dissertation presents my explorations in both molecular biology and science education research. In study one, we determined the ADIPOQ and ADIPORI genotypes of 364 White and 148 Black BrCa patients and used dominant model univariate logistic regression analyses to determine individual SNP and haplotype associations with tumor or patient characteristics in a case-case comparison. We found twelve associations between individual SNPs and patient or tumor characteristics that impact BrCa prognosis. For example, the ADIPOQ rs1501299 C allele was associated with ER+ tumors (OR=4.73, p=0.001) among White women >50 years of age at their time of diagnosis. Also, the A …