Life Sciences Commons^™

Inflammatory Cytokine Gene Expression In Mesenteric Adipose Tissue During Acute Experimental Colitis, William Conan Mustain, Marlene E. Starr, Joseph Daniel Valentino, Donald A. Cohen, Daiki Okamura, Chi Wang, B. Mark Evers, Hiroshi Saito

Markey Cancer Center Faculty Publications

BACKGROUND: Production of inflammatory cytokines by mesenteric adipose tissue (MAT) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Animal models of colitis have demonstrated inflammatory changes within MAT, but it is unclear if these changes occur in isolation or as part of a systemic adipose tissue response. It is also unknown what cell types are responsible for cytokine production within MAT. The present study was designed to determine whether cytokine production by MAT during experimental colitis is depot-specific, and also to identify the source of cytokine production within MAT.

METHODS: Experimental colitis was induced in 6-month-old C57BL/6 …

Effects Of Reproductive Condition, Roost Microclimate, And Weather Patterns On Summer Torpor Use By A Vespertilionid Bat, Joseph S. Johnson, Michael J. Lacki

Forestry and Natural Resources Faculty Publications

A growing number of mammal species are recognized as heterothermic, capable of maintaining a high-core body temperature or entering a state of metabolic suppression known as torpor. Small mammals can achieve large energetic savings when torpid, but they are also subject to ecological costs. Studying torpor use in an ecological and physiological context can help elucidate relative costs and benefits of torpor to different groups within a population. We measured skin temperatures of 46 adult Rafinesque's big-eared bats (Corynorhinus rafinesquii) to evaluate thermoregulatory strategies of a heterothermic small mammal during the reproductive season. We compared daily average and minimum skin …

Valproic Acid Causes Proteasomal Degradation Of Dicer And Influences Mirna Expression, Zhaiyi Zhang, Paolo Convertini, Manli Shen, Xiu Xu, Frédéric Lemoine, Pierre De La Grange, Douglas A. Andres, Stefan Stamm

Molecular and Cellular Biochemistry Faculty Publications

Valproic acid (VPA) is a commonly used drug to treat epilepsy and bipolar disorders. Known properties of VPA are inhibitions of histone deacetylases and activation of extracellular signal regulated kinases (ERK), which cannot fully explain VPA's clinical features. We found that VPA induces the proteasomal degradation of DICER, a key protein in the generation of micro RNAs. Unexpectedly, the concentration of several micro RNAs increases after VPA treatment, which is caused by the upregulation of their hosting genes prior to DICER degradation. The data suggest that a loss of DICER protein and changes in micro RNA concentration contributes to the …

Analgesic Tolerance Of Opioid Agonists In Mutant Mu-Opioid Receptors Expressed In Sensory Neurons Following Intrathecal Plasmid Gene Delivery, Guangwen Li, Fei Ma, Yanping Gu, Li-Yen Mae Huang

Physiology Faculty Publications

Background: Phosphorylation sites in the C-terminus of mu-opioid receptors (MORs) are known to play critical roles in the receptor functions. Our understanding of their participation in opioid analgesia is mostly based on studies of opioid effects on mutant receptors expressed in in vitro preparations, including cell lines, isolated neurons and brain slices. The behavioral consequences of the mutation have not been fully explored due to the complexity in studies of mutant receptors in vivo. To facilitate the determination of the contribution of phosphorylation sites in MOR to opioid-induced analgesic behaviors, we expressed mutant and wild-type human MORs (hMORs) in sensory …

Impact Of Noise On Molecular Network Inference, Radhakrishnan Nagarajan, Marco Scutari

Biostatistics Faculty Publications

Molecular entities work in concert as a system and mediate phenotypic outcomes and disease states. There has been recent interest in modelling the associations between molecular entities from their observed expression profiles as networks using a battery of algorithms. These networks have proven to be useful abstractions of the underlying pathways and signalling mechanisms. Noise is ubiquitous in molecular data and can have a pronounced effect on the inferred network. Noise can be an outcome of several factors including: inherent stochastic mechanisms at the molecular level, variation in the abundance of molecules, heterogeneity, sensitivity of the biological assay or measurement …

Forage News [2013-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Thank You Kentucky Farmers
• Forages at KCA
• Forage Variety Reports Now Available
• Southern Forages Translated Into Spanish
• Small Ruminant Grazing Conference to be held in Lexington on February 1, 2014
• AFGC Annual Conference - Memphis, TN
• 34th Kentucky Alfalfa Conference
• Updated Analysis on Fungicides and Alfalfa

Botryoccocus Braunii Triterpene Synthase Proteins And Nucleic Acid Molecules, And Methods For Their Use, Joe Chappell, Okada Shigeru, Tom Neihaus, Tim Devarenne

Plant and Soil Sciences Faculty Patents

This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.

Molecular Events In The Cell Types Of The Olfactory Epithelium During Adult Neurogenesis, Paula M. Heron, Arnold J. Stromberg, Patrick Breheny, Timothy S. Mcclintock

Physiology Faculty Publications

BACKGROUND: Adult neurogenesis, fundamental for cellular homeostasis in the mammalian olfactory epithelium, requires major shifts in gene expression to produce mature olfactory sensory neurons (OSNs) from multipotent progenitor cells. To understand these dynamic events requires identifying not only the genes involved but also the cell types that express each gene. Only then can the interrelationships of the encoded proteins reveal the sequences of molecular events that control the plasticity of the adult olfactory epithelium.

RESULTS: Of 4,057 differentially abundant mRNAs at 5 days after lesion-induced OSN replacement in adult mice, 2,334 were decreased mRNAs expressed by mature OSNs. Of the …

A Process Similar To Autophagy Is Associated With Cytocidal Chloroquine Resistance In Plasmodium Falciparum, David Gaviria, Michelle F. Paguio, Lindsey B. Turnbull, Asako Tan, Amila Siriwardana, Debasish Ghosh, Michael T. Ferdig, Anthony P. Sinai, Paul D. Roepe

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Resistance to the cytostatic activity of the antimalarial drug chloroquine (CQ) is becoming well understood, however, resistance to cytocidal effects of CQ is largely unexplored. We find that PfCRT mutations that almost fully recapitulate P. falciparum cytostatic CQ resistance (CQR(CS)) as quantified by CQ IC50 shift, account for only 10-20% of cytocidal CQR (CQR(CC)) as quantified by CQ LD50 shift. Quantitative trait loci (QTL) analysis of the progeny of a chloroquine sensitive (CQS; strain HB3)×chloroquine resistant (CQR; strain Dd2) genetic cross identifies distinct genetic architectures for CQR(CS) vs CQR(CC) phenotypes, including identification of novel interacting chromosomal loci that influence CQ …

Molecular Cloning And Characterization Of A P-Glycoprotein From The Diamondback Moth, Plutella Xylostella (Lepidoptera: Plutellidae), Lixia Tian, Jiaqiang Yang, Wenjie Hou, Baoyun Xu, Wen Xie, Shaoli Wang, Youjun Zhang, Xuguo Zhou, Qingjun Wu

Entomology Faculty Publications

Macrocyclic lactones such as abamectin and ivermectin constitute an important class of broad-spectrum insecticides. Widespread resistance to synthetic insecticides, including abamectin and ivermectin, poses a serious threat to the management of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major pest of cruciferous plants worldwide. P-glycoprotein (Pgp), a member of the ABC transporter superfamily, plays a crucial role in the removal of amphiphilic xenobiotics, suggesting a mechanism for drug resistance in target organisms. In this study, PxPgp1, a putative Pgp gene from P. xylostella, was cloned and characterized. The open reading frame (ORF) of PxPgp1 consists of 3774 …

Early Flowering Mutant Chia And Uses Thereof, David Hildebrand, Watchareewan Jamboonsri, Timothy Phillips

Plant and Soil Sciences Faculty Patents

The invention relates to early flowering Chia (Salvia hispanica L.) strains that are suitable for culturing in a temperate area for seed production. Mutations are introduced to wild type chia seeds. Desired mutant progeny having normal appearance and an altered flora organ development is subsequently identified.

An Intergenic Region Shared By At4g35985 And At4g35987 In Arabidopsis Thaliana Is A Tissue Specific And Stress Inducible Bidirectional Promoter Analyzed In Transgenic Arabidopsis And Tobacco Plants, Joydeep Banerjee, Dipak K. Sahoo, Nrisingha Dey, Robert Houtz, Indu B. Maiti

Kentucky Tobacco Research and Development Center Faculty Publications

On chromosome 4 in the Arabidopsis genome, two neighboring genes (calmodulin methyl transferase At4g35987 and senescence associated gene At4g35985) are located in a head-to-head divergent orientation sharing a putative bidirectional promoter. This 1258 bp intergenic region contains a number of environmental stress responsive and tissue specific cis-regulatory elements. Transcript analysis of At4g35985 and At4g35987 genes by quantitative real time PCR showed tissue specific and stress inducible expression profiles. We tested the bidirectional promoter-function of the intergenic region shared by the divergent genes At4g35985 and At4g35987 using two reporter genes (GFP and GUS) in both orientations in transient tobacco protoplast and …

Evidence For Finely-Regulated Asynchronous Growth Of Toxoplasma Gondii Cysts Based On Data-Driven Model Selection, Adam M. Sullivan, Xiaopeng Zhao, Yasuhiro Suzuki, Eri Ochiai, Stephen Crutcher, Michael A. Gilchrist

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii establishes a chronic infection by forming cysts preferentially in the brain. This chronic infection is one of the most common parasitic infections in humans and can be reactivated to develop life-threatening toxoplasmic encephalitis in immunocompromised patients. Host-pathogen interactions during the chronic infection include growth of the cysts and their removal by both natural rupture and elimination by the immune system. Analyzing these interactions is important for understanding the pathogenesis of this common infection. We developed a differential equation framework of cyst growth and employed Akaike Information Criteria (AIC) to determine the growth and removal functions that best describe …

Intracellular Recording, Sensory Field Mapping, And Culturing Identified Neurons In The Leech, Hirudo Medicinalis, Josh Titlow, Zana R. Majeed, John G. Nicholls, Robin L. Cooper

Biology Faculty Publications

The freshwater leech, Hirudo medicinalis, is a versatile model organism that has been used to address scientific questions in the fields of neurophysiology, neuroethology, and developmental biology. The goal of this report is to consolidate experimental techniques from the leech system into a single article that will be of use to physiologists with expertise in other nervous system preparations, or to biology students with little or no electrophysiology experience. We demonstrate how to dissect the leech for recording intracellularly from identified neural circuits in the ganglion. Next we show how individual cells of known function can be removed from …

Neural Circuit Recording From An Intact Cockroach Nervous System, Josh Titlow, Zana R. Majeed, H. Bernard Hartman, Ellen Burns, Robin L. Cooper

Biology Faculty Publications

The cockroach ventral nerve cord preparation is a tractable system for neuroethology experiments, neural network modeling, and testing the physiological effects of insecticides. This article describes the scope of cockroach sensory modalities that can be used to assay how an insect nervous system responds to environmental perturbations. Emphasis here is on the escape behavior mediated by cerci to giant fiber transmission in Periplaneta americana. This in situ preparation requires only moderate dissecting skill and electrophysiological expertise to generate reproducible recordings of neuronal activity. Peptides or other chemical reagents can then be applied directly to the nervous system in solution …

Forage News [2013-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Jimmy Ray May, Sr. 1943-2013
• KFGC Awards
• 2013 KFGC Forage Spokesperson Contest
• Small Ruminant Grazing Conference to be held in Lexington on February 1, 2014
• Grass - A Pandrama in Words
• Grass-based Health
• Timing of Grazing Effects on Pasture Productivity
• Hay Bale Fires Still a Threat
• Minnesota Dairy Named Forage Superbowl Grand Champ

Epigenetic Dominance Of Prion Conformers, Eri Saijo, Hae-Eun Kang, Jifeng Bian, Kristi G. Bowling, Shawn Browning, Sehun Kim, Nora Hunter, Glenn C. Telling

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Although they share certain biological properties with nucleic acid based infectious agents, prions, the causative agents of invariably fatal, transmissible neurodegenerative disorders such as bovine spongiform encephalopathy, sheep scrapie, and human Creutzfeldt Jakob disease, propagate by conformational templating of host encoded proteins. Once thought to be unique to these diseases, this mechanism is now recognized as a ubiquitous means of information transfer in biological systems, including other protein misfolding disorders such as those causing Alzheimer's and Parkinson's diseases. To address the poorly understood mechanism by which host prion protein (PrP) primary structures interact with distinct prion conformations to influence pathogenesis, …

Proprioception And Tension Receptors In Crab Limbs: Student Laboratory Exercises, Zana R. Majeed, Josh Titlow, H. Bernard Hartman, Robin L. Cooper

Biology Faculty Publications

The primary purpose of these procedures is to demonstrate for teaching and research purposes how to record the activity of living primary sensory neurons responsible for proprioception as they are detecting joint position and movement, and muscle tension. Electrical activity from crustacean proprioceptors and tension receptors is recorded by basic neurophysiological instrumentation, and a transducer is used to simultaneously measure force that is generated by stimulating a motor nerve. In addition, we demonstrate how to stain the neurons for a quick assessment of their anatomical arrangement or for permanent fixation. Staining reveals anatomical organization that is representative of chordotonal organs …

Identification And Developmental Profiling Of Micrornas In Diamondback Moth, Plutellaxylostella (L.), Pei Liang, Bing Feng, Xuguo Zhou, Xiwu Gao

Entomology Faculty Publications

MicroRNAs (miRNAs) are a group of small RNAs involved in various biological processes through negative regulation of mRNAs at the post-transcriptional level. Although miRNA profiles have been documented in over two dozen insect species, few are agricultural pests. In this study, both conserved and novel miRNAs in the diamondback moth, Plutella xylostella L., a devastating insect pest of cruciferous crops worldwide, were documented. High-throughput sequencing of a small RNA library constructed from a mixed life stages of P. xylostella, including eggs, 1st to 4th (last) instar larvae, pupae and adults, identified 384 miRNAs, of which 174 were P. xylostella specific. …

Kentucky Forage Spokesperson Contest, Kentucky Forage And Grassland Council

Kentucky Grazing Conference

No abstract provided.

Can We Graze 300+ Days?, Glen Aiken

Kentucky Grazing Conference

Dependence on stored feeds during the winter months has been necessary in order to maintain body condition and meet nutrient requirements of cattle herds; unfortunately, cost of stored feeds typically are a major part of the farm budget. We know, all too well, that hay shortages and unstable feed ingredient markets have reduced profit potential in cattle production. This has further placed higher demands on supplies of co-product feeds to cause prices of these alternative feeds to rise and make them less cost effective. There will likely be less reliance on concentrate and co-product feeds as the forage-based livestock industry …

Pastures For Horses, Robert Coleman

Kentucky Grazing Conference

The horse evolved as a grazing animal. In order to consume enough forage to meet the horses’ nutritional needs a significant amount of time would be spent each day grazing. The pattern of selecting forage-moving to the next area starting to graze again can easily translate into 14 to 16 hours of grazing activity each day. The level of grazing activity will also be affected by the available forage and the daily access to pasture.

Pastures For Goats And Sheep, Greg Brann

Kentucky Grazing Conference

Pasture for sheep is not that different than that for cattle but pasture for goats is very different. Sheep are grazers like cattle whereas goats are browsers like deer. Even though grass and clover are not a goats preference if all of the forage present is grass and clover goats will eat it. Due to goats and sheep being more prey animals than cattle they don’t like to wade off into tall vegetation, also it takes more energy for them to move through tall growth. Many plants that we traditionally consider weeds become desirable forbs when goats or sheep are …

The Role Of Weed Control In Profitable Pastures, Scott Flynn, Pat Burch

Kentucky Grazing Conference

Pasture weeds compete for resources such as space, water, and nutrients. This competition reduces forage yield which in turn reduces the carrying capacity of a pasture. Although herbicide applications are needed to gain a quick upper hand on weed control most producers are deterred due to cost, the potential loss of pasture legumes, labor, or the belief that herbicide applications are required frequently to keep weed population under control. However, herbicide use in pastures should not be looked at in this manner but instead as an investment that can pay for itself very quickly and easily, and be long-term especially …

Role Of Legumes In Pastures, Garry D. Lacefield

Kentucky Grazing Conference

Successful livestock production is dependent on forage programs which supply large quantities of adequate quality, homegrown feed. A major percentage of the feed units for beef (83%) and dairy (61%) cattle come from forages. In addition, forages supply an estimated 91%, 72%, 15% and 99% of the nutrients consumed by sheep and goats, horses, swine and ruminant wildlife, respectively.

(How I Think About) Kentucky's Pastures: One Of Kentucky's Untapped Resources, Jimmy C. Henning

Kentucky Grazing Conference

One of the privileges of old(er) age is the prerogative to take liberties with assigned tasks. At the risk of confusing producers and offending more scientifically-current forage scientists, the topic of pasture as an untapped resource presented an opportunity to address task from an unexpected angle. Certainly, Kentucky’s pastures are a resource with untapped potential. But the idea that their value as a resource depends on how we think about them is one worth pursuing.

Foreword And Kfgc Award Winners [2013], Garry D. Lacefield, Christi L. Forsythe

Kentucky Grazing Conference

No abstract provided.

Sorghum Mutant Rg Displays Antithetic Leaf Shoot Lignin Accumulation Resulting In Improved Stem Saccharification Properties, Carloalberto Petti, Anne E. Harman-Ware, Mizuki Tateno, Rekha Kushwaha, Andrew Shearer, A. Bruce Downie, Mark Crocker, Seth Debolt

Horticulture Faculty Publications

BACKGROUND: Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock.

RESULTS: It was found that RG displayed increased accumulation of lignin in leaves and depletion in the stems, antithetic to the trend observed in wild type. Consistent with these measurements, the RG leaf tissue displayed reduced saccharification efficiency whereas the stem saccharification efficiency increased relative to wild type. Reduced lignin was linked to improved saccharification in RG stems, but a chemical shift to greater S:G ratios in RG stem …

Construction Of A Sonchus Yellow Net Virus Minireplicon: A Step Toward Reverse Genetic Analysis Of Plant Negative-Strand Rna Viruses, Uma Ganesan, Jennifer N. Bragg, Min Deng, Sharon Marr, Mi Yeon Lee, Shasha Qian, Manling Shi, Justin Kappel, Cole Peters, Yeon Lee, Michael M. Goodin, Ralf G. Dietzgen, Zhenghe Li, Andrew O. Jackson

Plant Pathology Faculty Publications

Reverse genetic analyses of negative-strand RNA (NSR) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant NSR viruses. To develop a reverse genetic approach for analysis of plant NSR viruses, we have engineered Sonchus yellow net nucleorhabdovirus (SYNV) minireplicon (MR) reporter cassettes for Agrobacterium tumefaciens expression in Nicotiana benthamiana leaves. Fluorescent reporter genes substituted for the SYNV N and P protein open reading frames (ORFs) exhibited intense single-cell foci throughout regions of infiltrated leaves expressing the SYNV MR derivatives and the SYNV nucleocapsid (N), phosphoprotein …

Forage News [2013-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 14th Kentucky Grazing Conference - October 10
• Kentuckians attend IGC
• Clayton Geralds elected to National Hay Association Board
• NAFA's 2013 Alfalfa Intensive Training Seminar (AITS) - Register Today!
• Prussic Acid Poisoning and Frost
• AFGC to meet in Memphis
• New Research Leader, Forage-Animal Production Research Unit, Lexington, Kentucky Announced
• NRCS Technical Service Provider Program
• Historic Agricultural Data Now Online
• Mobile App Translates Per-Bale to Per-Ton Price