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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Biochemical Investigations Of Macular Degeneration: The Significance Of Protein Oxidation Including Novel Methods For Its Study, Sarah Warburton
Biochemical Investigations Of Macular Degeneration: The Significance Of Protein Oxidation Including Novel Methods For Its Study, Sarah Warburton
Theses and Dissertations
The retinal pigment epithelium (RPE) is a monolayer of cells located directly behind the photoreceptor cells in the retina. These cells are involved in a variety of functions that support the visual process in the eye, namely 1) they form a blood-retina barrier which separates the neural retina from the choroid's blood supply, 2) the apical processes of RPE cells diurnally phagocytose the outer segments of photoreceptor cells, and 3) they participate in the renewal of the photopigment 11-cis retinal. Age-related macular degneration (AMD) is the leading cause of blindness in people over the age of 50 years in North …
The Structure And Function Of Frataxin, Krisztina Z. Bencze, Kalyan C. Kondapalli, Jeremy D. Cook, Stephen Mcmahon, César Millán-Pacheco, Nina Pastor, Timothy L. Stemmler
The Structure And Function Of Frataxin, Krisztina Z. Bencze, Kalyan C. Kondapalli, Jeremy D. Cook, Stephen Mcmahon, César Millán-Pacheco, Nina Pastor, Timothy L. Stemmler
Biochemistry and Molecular Biology Faculty Publications
Frataxin, a highly conserved protein found in prokaryotes and eukaryotes, is required for efficient regulation of cellular iron homeostasis. Humans with a frataxin deficiency have the cardio- and neurodegenerative disorder Friedreich’s ataxia, commonly resulting from a GAA trinucleotide repeat expansion in the frataxin gene. While frataxin’s specific function remains a point of controversy, a general consensus is the protein assists in controlling cellular iron homeostasis by directly binding iron. This review focuses on the structural and biochemical aspects of iron binding by the frataxin orthologs and outlines molecular attributes that may help explain the protein’s role in different cellular pathways.
In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng
In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng
Honors Scholar Theses
The major histocompatibility complex (MHC) is a gene family responsible for many critical functions of the immune system in most vertebrates. The MHC consists of three classes differentiated by their structure and function, and MHC class I encodes antigen binding proteins as well as chaperone and accessory proteins such as tapasin. The purpose of this project is to reconstitute several human MHC class I molecules in their peptide-filled and peptide-deficient forms, and to purify these proteins for biochemical study. The expressed proteins include wild type and mutant variants of the fusion protein human leukocyte antigen HLA-B*0801-fos, and human beta-2-microglobulin (β2m). …
Three-Dimensional Structure Of The Bacterial Cell Wall Peptidoglycan, Samy O. Meroueh, Krisztina Z. Bencze, Dusan Hesek, Mijoon Lee, Timothy L. Stemmler, Shahriar Mobashery
Three-Dimensional Structure Of The Bacterial Cell Wall Peptidoglycan, Samy O. Meroueh, Krisztina Z. Bencze, Dusan Hesek, Mijoon Lee, Timothy L. Stemmler, Shahriar Mobashery
Biochemistry and Molecular Biology Faculty Publications
The 3D structure of the bacterial peptidoglycan, the major constit- uent of the cell wall, is one of the most important, yet still unsolved, structural problems in biochemistry. The peptidoglycan comprises alternating N-acetylglucosamine (NAG) and N-acetylmu- ramic disaccharide (NAM) saccharides, the latter of which has a peptide stem. Adjacent peptide stems are cross-linked by the transpeptidase enzymes of cell wall biosynthesis to provide the cell wall polymer with the structural integrity required by the bacte- rium. The cell wall and its biosynthetic enzymes are targets of antibiotics. The 3D structure of the cell wall has been elusive because of its …
Coordinated Effects Of Distal Mutations On Environmentally Coupled Tunneling In Dihydrofolate Reductase, Lin Wang, Nina M. Goodey, Stephen J. Benkovic, Amnon Kohen
Coordinated Effects Of Distal Mutations On Environmentally Coupled Tunneling In Dihydrofolate Reductase, Lin Wang, Nina M. Goodey, Stephen J. Benkovic, Amnon Kohen
Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works
One of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance the catalyzed chemical transformation. In this study, dihydrofolate reductase, a small monomeric protein that catalyzes a single C-H-C transfer, is used as a model system to address this question. Experimental and computational studies have proposed a dynamic network that includes two residues remote from the active site (G121 and M42). The current study compares the nature of the H-transfer step of the WT enzyme, two single mutants, and their double mutant. The contribution of quantum mechanical tunneling and enzyme dynamics to the H-transfer step …
Monosaccharide Interactions With Rh(Iii) Cis-Bipyridine Complexes, Sarah M. Lane
Monosaccharide Interactions With Rh(Iii) Cis-Bipyridine Complexes, Sarah M. Lane
Undergraduate Review
No abstract provided.