Life Sciences Commons^™

Lake Mead National Recreation Area Monitoring And Evaluation Of Sensitive Wildlife: Quarterly Progress Report, Period Ending December 31, 2006, Margaret N. Rees

Wildlife Monitoring

• Research assistant hired for Relict Leopard Frog conservation project.
• High school minority intern hired to assist with research efforts.
• Nocturnal visual encounter surveys for Relict Leopard Frogs conducted at all established natural sites and at 6 of 7 translocation sites.
• Vegetation management conducted to decrease tamarisk cover along the stream at the Pupfish Refuge Spring – a Relict Leopard Frog translocation site.
• New draft guidelines and field count protocols developed for midwinter bald eagle count.
• Preliminary analysis and modeling of thrasher habitat selection conducted and sampling assessed
• Call-broadcast surveys for thrasher species conducted at 43 points countywide, focusing on vegetation …

Lake Mead National Recreation Area Vegetation Monitoring And Management: Quarterly Progress Report, Period Ending December 31, 2006, Margaret N. Rees

Vegetation Monitoring

Executive Summary

• Two new Weed Sentry research assistants were hired.
• Weed Sentry staff surveyed for exotic species on 89 miles of roads on NPS and BLM land and treated more than 21,000 exotic plants in incipient populations.
• A grid-based rare plant monitoring method was tested this quarter.
• A manuscript detailing vegetation succession on a water pipeline at Lake Mead NRA was submitted for review to the journal Crossosoma.
• New integrative projects undertaken this quarter included establishing a competition study between a native grass and the exotic Sahara mustard, salvaging plants for research purposes from private sites with permission from landowners, …

Oliver Ranch Science School Complex & Wild Horse And Burro Facility: Final Close-Out Report, Margaret N. Rees

Oliver Ranch Project

“The mission of the Red Rock Desert Learning Center is to instill stewardship and respect by increasing knowledge and understanding of the Mojave Desert ecosystems and cultures through a unique experiential discovery program.”

Nroc Coordination And Program Delivery, Amanda Stone, Frank Mitchell

PREP Reports & Publications

The primary goal of the Natural Resources Outreach Coalition (NROC) program is to provide education and outreach to communities in the NH Coastal Watersheds that are dealing with the effects of growth, and looking for ways to conserve open spaces and natural resources. Program objectives to meet this goal include: 1. NROC and client communities determine natural resource concerns of the communities. 2. NROC and client communities gather community information about these concerns 3. NROC develops and client communities host community presentations about natural resource-based planning. 4. Communities receive follow up technical and educational assistance as requested. In 2006, NROC …

Cadmium Toxicity On Arterioles Vascular Smooth Muscle Cells Of Spontaneously Hypertensive Rats, Benny Washington, Shunta Williams, Patrice Armstrong, Charlie Mtshali, John T. Robinson, Elbert L. Myles

Biology Faculty Research

Cadmium (Cd) is frequently used in various industrial applications and is a ubiquitous environmental toxicant, also present in tobacco smoke. An important route of exposure is the circulatory system whereas blood vessels are considered to be main stream organs of Cd toxicity. Our previous results indicate that cadmium chloride (CdCl2) affects mean arterial blood pressure in hypertensive rats. We hypothesized that Cd alters the intracellular calcium transient mechanism, by cadmium-induced stimulation of MAPKs (ERK 1 & 2) which is mediated partially through calcium-dependent PKC mechanism. To investigate this hypothesis, we exposed primary cultures of vascular smooth muscle cells (VSMCs) from …

Shoreland Buffer Module For Granit Data Mapper, Fay A. Rubin, David G. Justice

PREP Reports & Publications

The Complex Systems Research Center at the University of New Hampshire enhanced the GRANIT Data Mapper (http://mapper.granit.unh.edu) by incorporating data describing shoreline buffers in New Hampshire. The project supports an ongoing, comprehensive New Hampshire Estuaries Project (NHEP) outreach initiative that seeks to educate municipal decision-makers about the importance of stream buffers in preserving water quality in coastal New Hampshire. It complements these existing outreach efforts by allowing coastal managers, local land use boards, and the general public to readily visualize the spatial extent of current and/or proposed shoreline regulations in their community. The primary data source for the analysis was …

Shellfish Tissue Monitoring In New Hampshire Estuaries 2005, Phil Trowbridge

PREP Reports & Publications

Conducted by a committee of Canadian and US government and university scientists, Gulfwatch examines the effects of decades of development and industrialization on the water quality of the Gulf as it relates to human health as well as its impact on other marine organisms. Gulfwatch scientists collect blue mussels at over 60 US and Canadian sites Gulf-wide, and analyze the organisms’ tissue for potentially harmful levels and concentrations of toxins including heavy metals, chlorinated pesticides, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs). New Hampshire increased the number of Gulfwatch sampling locations from two sites per year in 1997 to …

Juvenile Soft-Shell Clam, Mya Arenaria L.Research In The Hampton-Seabrook Estuary, Brian F. Beal

PREP Reports & Publications

A series of field experiments was conducted at two intertidal sites in the Hampton Seabrook Estuary from November 2004-2006 to assess the efficacy of enhancing intertidal areas with cultured clam (Mya arenaria L.) seed (mean shell length [SL] = 7-10 mm). Measurement variables in each experiment included survival and growth of both cultured and wild seed clams. The first of three trials (November 2004 - May 2005) examined the interactive effects of size of planting area (4 m2, 8 m2, 12 m2, and 18 m2) and predator deterrent netting (none, 4.2 mm, and 6.4 mm aperture [flexible, plastic netting]) at …

Town Of Strafford Nroc Projects, Carolyn Page, Al Pratt, Jeff Schloss, Harmony Anderson

PREP Reports & Publications

The three Strafford committees formed through the work with the Natural Resources Outreach Coalition in 2004 and supported by the grant award from the New Hampshire Estuaries Project have succeeded in furthering land protection, water quality protection, and managing growth here in Strafford. The original NROC meetings brought many new volunteer citizens into the process, but even their enthusiasm and willingness to work could not have earned these results without the financial support of the NHEP grant award. Hours of letter writing and personal contact with landowners by the volunteers of the Land Protection Group have raised awareness of the …

Recent Advances In Basic Neurosciences And Brain Disease: From Synapses To Behavior, Guo-Qiang Bi, Vadim Bolshakov, Guojun Bu, Catherine M. Cahill, Zhou-Feng Chen, Graham L. Collingridge, Robin L. Cooper, Jens R. Coorssen, Alaa El-Husseini, Vasco Galhardo, Wen-Biao Gan, Jianguo Gu, Kazuhide Inoue, John Isaac, Koichi Iwata, Zhengping Jia, Bong-Kiun Kaang, Mikito Kawamata, Satoshi Kida, Eric Klann, Tatsuro Kohno, Min Li, Xiao-Jiang Li, John F. Macdonald, Karim Nader, Peter V. Nguyen, Uhtaek Oh, Ke Ren, John C. Roder, Michael W. Salter, Weihong Song, Shuzo Sugita, Shao-Jun Tang, Yuanxiang Tao, Yu Tian Wang, Newton Woo, Melanie A. Woodin, Zhen Yan, Megumu Yoshimura, Ming Xu, Zao C. Xu, Xia Zhang, Mei Zhen, Min Zhuo

Biology Faculty Publications

Understanding basic neuronal mechanisms hold the hope for future treatment of brain disease. The 1st international conference on synapse, memory, drug addiction and pain was held in beautiful downtown Toronto, Canada on August 21-23, 2006. Unlike other traditional conferences, this new meeting focused on three major aims: (1) to promote new and cutting edge research in neuroscience; (2) to encourage international information exchange and scientific collaborations; and (3) to provide a platform for active scientists to discuss new findings. Up to 64 investigators presented their recent discoveries, from basic synaptic mechanisms to genes related to human brain disease. This meeting …

Switchgrass For Biomass Feedstock In The Usa, Matt Sanderson, Paul Adler, Akwasi Boateng, Michael Casler, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass has received much study for biomass feedstock production and conversion through research funded by the US-DOE and USDA during the past two decades. We have an improved understanding of the adaptation of existing cultivars and new cultivars with yield and adaptation improvements are now appearing. We also have a good understanding of the agronomics of switchgrass production, mainly from its use as a forage crop. There remain several constraints to switchgrass use in bioenergy cropping systems, including reliable establishment methods to obtain productive stands in the first year, targeted fertilization and nutrient management techniques to efficiently use nitrogen fertilizer, …

Information And Data Management Program's Pi Workshop, Peggy Agouris

University of Maine Office of Research Administration: Grant Reports

The objective of this workshop is to bring together the PIs and Co-PIs currently funded by the Information and Data Management Program of the National Science Foundation to discuss and exchange ideas on the focus topics of their field, as well as to identify and elaborate on emerging themes and particular emphases for future activities.

More specifically, the researchers, along with selected industry and government invitees, cooperatively focused on:

(1) analyzing research and development issues fundamental in making progress towards new challenges imposed by such diverse data sources as the Internet, embedded and distributed sensors, and satellites;

(2) specifying areas …

Information And Data Management Program's Pi Workshop, Peggy Agouris

University of Maine Office of Research Administration: Grant Reports

The objective of this workshop is to bring together the PIs and Co-PIs currently funded by the Information and Data Management Program of the National Science Foundation to discuss and exchange ideas on the focus topics of their field, as well as to identify and elaborate on emerging themes and particular emphases for future activities.

More specifically, the researchers, along with selected industry and government invitees, cooperatively focused on:

(1) analyzing research and development issues fundamental in making progress towards new challenges imposed by such diverse data sources as the Internet, embedded and distributed sensors, and satellites;

(2) specifying areas …

Cains Brook And Mill Creek Watershed Management Plan, Town Of Seabrook, Waterfront Engineers, Inc.

PREP Reports & Publications

Cains Brook is a freshwater stream that flows in an easterly direction from its origin—a spring fed pond about one mile west of I-95 on the Salisbury, Massachusetts/Seabrook, New Hampshire border. At its lower reaches the brook is joined by Shepard Brook where it becomes Mill Creek, a tidal creek that discharges into the Hampton-Seabrook estuary. Along its course there are several ponds on either side of US Route 1—Secords, Cains and Mary’s Ponds to the west of Route 1 and Cains Mill Pond and Noyes Pond to the east. This stream system is approximately 3.8 miles long. The Cains …

Properties Of Corneal Receptors In A Teleost Fish, Paul J. Ashley, Lynne U. Sneddon, Catherine R. Mccrohan

Veterinary Science and Medicine Collection

Corneal receptors have not previously been identified in lower vertebrates. The present study describes the properties of trigeminal ganglion corneal receptors in a teleost fish, the rainbow trout (Oncoryhnchus mykiss). Out of 27 receptors, 7 were polymodal nociceptors, 6 were mechanothermal nociceptors, 2 were mechanochemical receptors and the largest group, 12, were only responsive to mechanical stimulation. No cold responsive receptors were found on the trout cornea. Mechanical and thermal thresholds were lower and receptive field diameters smaller than those of cutaneous trigeminal receptors in the trout, demonstrating greater sensitivity in the cornea. The lack of cold sensitive neurons may …

Land Conservation Plan For New Hampshire’S Coastal Watersheds – Implementation & Outreach, Mark Zankel

PREP Reports & Publications

The Nature Conservancy was awarded funding to complete activities to promote the Land Conservation Plan for New Hampshire’s Coastal Watersheds in the fall of 2006. The Nature Conservancy and its partners, Society for the Protection of New Hampshire Forests, Rockingham Planning Commission, and Strafford Regional Planning Commission, conducted the following tasks to increase understanding and use of the plan by communities, land trusts and others interested in land protection.

Recombinant Watermelon (Citrullus Lanatus) Hydroperoxide Lyase And Uses Thereof, David Hildebrand, Hirotada Fukushige

Plant and Soil Sciences Faculty Patents

Recombinant watermelon (Citrullus lanatus) hydroperoxide lyase protein, DNA sequences encoding the protein, vectors containing the DNA sequences and hosts containing the vectors are provided, together with methods for recombinantly producing watermelon hydroperoxide lyase, DNA sequences, vectors and hosts.

Untemplated Oligoadenylation Promotes Degradation Of Risc-Cleaved Transcripts, Fadia Ibrahim, Jennifer Rohr, Won-Joong Jeong, Jennifer Hesson, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty and Staff Publications

In the best-characterized mechanism of RNAmediated silencing, small interfering RNAs (siRNAs), incorporated into the RNA-induced silencing complex (RISC), guide the endonucleolytic cleavage of complementary RNAs (1). In Drosophila melanogaster, these RISC-generated products are eventually degraded by exoribonucleases: Xrn1, a 5′-to-3′ exonuclease, and exosome, a 3′-to-5′ multisubunit exonuclease (2). Interestingly, in Arabidopsis thaliana and in mammals, an oligouridine or oligoadenine [oligo(U/A)] tail is added to the 5′ RNA fragments resulting from microRNA-directed cleavage (3). However, the biological role of this tail remains unclear.

The Plant Structure Ontology, A Unified Vocabulary Of Anatomy And Morphology Of A Flowering Plant, Katica Ilic, Elizabeth Kellogg, Pankaj Jaiswal, Felipe Zapata, Peter Stevens, Leszek Vincent, Shulamit Avraham, Leonore Reiser, Anuradha Pujar, Martin Sachs, Noah Whitman, Susan Mccouch, Mary Schaeffer, Doreen Ware, Lincoln Stein, Seung Rhee

Biology Department Faculty Works

Formal description of plant phenotypes and standardized annotation of gene expression and protein localization data require uniform terminology that accurately describes plant anatomy and morphology. This facilitates cross species comparative studies and quantitative comparison of phenotypes and expression patterns. A major drawback is variable terminology that is used to describe plant anatomy and morphology in publications and genomic databases for different species. The same terms are sometimes applied to different plant structures in different taxonomic groups. Conversely, similar structures are named by their species-specific terms. To address this problem, we created the Plant Structure Ontology (PSO), the first generic ontological …

Spatial Variation In The Chemical Composition Of Natal Otholiths From A Reef Fish In The Galápagos Islands, Benjamin I. Ruttenberg, Robert R. Warner

Biological Sciences

Over the past decade, researchers have used variation in the chemical composition of fish otoliths (earstones) to address a number of ecological questions, such as stock assessment and assessing rates of movement of individuals among habitats or life-history stages. However, these methods have yet to be applied successfully to the study of larval connectivity, a major unanswered question in marine ecology. Accomplishing this task requires a reference collection of pre-pelagic natal otoliths and accurate measurement of the chemical signatures of otoliths cores of recruits. We analyzed the chemical composition of natal otoliths from near-term benthic eggs of a damselfish (Stegastes …

High Sensitivity Rna Pseudoknot Prediction, Xiaolu Huang, Hesham Ali

Information Systems and Quantitative Analysis Faculty Publications

Most ab initio pseudoknot predicting methods provide very few folding scenarios for a given RNA sequence and have low sensitivities. RNA researchers, in many cases, would rather sacrifice the specificity for a much higher sensitivity for pseudoknot detection. In this study, we introduce the Pseudoknot Local Motif Model and Dynamic Partner Sequence Stacking (PLMM_DPSS) algorithm which predicts all PLM model pseudoknots within an RNA sequence in a neighboring-region-interferencefree fashion. The PLM model is derived from the existing Pseudobase entries. The innovative DPSS approach calculates the optimally lowest stacking energy between two partner sequences. Combined with the Mfold, PLMM_DPSS can also …

Caenorhabditis Briggsae Methods, Scott Everet Baird, Helen M. Chamberlin

Biological Sciences Faculty Publications

Caenorhabditis briggsae is being developed in parallel to C. elegans as a model system, primarily for the study of evolution. Like C. elegans, C. briggsae is a protandrous hermaphrodite and like C. elegans, its genome has been sequenced. From this point, these two model systems diverge. The development, behavior, and physiology of C. elegans have been characterized through tens of thousands of genetic and molecular studies. Genetic and molecular characterizations of C. briggsae are relatively few. Experimental resources in C. elegans include a high density recombination map that is well integrated with the genome sequence. The C. briggsae …

Aged Rats: Sex Differences And Responses To Chronic Stress, Rachel E. Bowman, Neil J. Maclusky, Samantha Diaz-Weinstein, Mark C. Zrull, Victoria N. Luine

Psychology Faculty Publications

Cognitive, as well as physiological, sex differences exist in young adult rats under both basal conditions and following chronic stress; however, few studies have examined whether sex differences remain in aged subjects and whether responses to stress are altered. We compared aged male and female Fischer 344 rats (21.5 months at testing) without stress and when given 21 days of restraint for 6 h/day on locomotion, anxiety-related behaviors, object recognition (non-spatial memory), object placement (spatial memory), body weight and serum steroid hormone levels. Control (unstressed) females had lower levels of estradiol and testosterone and higher corticosterone than males, and stress …

Computational Promoter Analysis Of Mouse, Rat, And Human Antimicrobial Peptide-Coding Genes, Chin-Yo Lin, Manisha Brahmachary, Christian Schonbach, Liang Yang, Enli Huang, Sin Lam Tan, Rajesh Chowdhary, S. P. T. Krishnan, David A. Hume, Chikatoshi Kai, Jun Kawai, Piero Carninci, Yoshihide Hayashizaki, Vladimir B. Bajic

Faculty Publications

Mammalian antimicrobial peptides (AMPs) are effectors of the innate immune response. A multitude of signals coming from pathways of mammalian pathogen/pattern recognition receptors and other proteins affect the expression of AMP-coding genes (AMPcgs). For many AMPcgs the promoter elements and transcription factors that control their tissue cell-specific expression have yet to be fully identified and characterized. Results- Based upon the RIKEN full-length cDNA and public sequence data derived from human, mouse and rat, we identified 178 candidate AMP transcripts derived from 61 genes belonging to 29 AMP families. However, only for 31 mouse genes belonging to 22 AMP families we …

West River Ag Center Crops And Soils Research Annual Progress Report, 2006, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2006 progress report of the West River Crops and Soils Research Projects, South Dakota Agricultural Experiment Station. This document includes reports on: weather and climate, wheat and grain variety trials, management and tillage, and weed and pest control.

Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2006, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2006 progress report for the Central Crops and Soils Research Station at Highmore, South Dakota State College. This document represents the research conducted at the Station during the 2006 crop season including: temperature and precipitation data, evaluation of native and naturalized grasses for reduced-input turf in the Northern Plains, 2006 NTEP tall fescue ancillary trial for drought tolerance, field evaluations of woody plant materials, alfalfa production, optimal management of drought -tolerant legumes and warm season annual grasses, winter wheat breeding, evaluation of sunflower germplasm for resistance to the red sunflower seed weevil, weed control, fertilizer influence on …

Advancing The Methodology For Cyclopenta[C]Thiophene Sythesis: A Step Toward Anit-Leukemia Agents And Improved Semiconductiors, Riley Jones

Mahurin Honors College Capstone Experience/Thesis Projects

Metal n5-cyclopenta[c]thienyl complexes are of significant interest in both catalysis and materials chemistry. These relatives of the low-band-gap polymer polybenzo[2,3-c]thiophene show great promise as environmentally stable conductive polymers and as energy-efficient light-emitting diodes (LEDs) due to their unique electronic properties. Recently, studies have shown that cyclopenta[c]thiophene molecules are effective as photodynamic anticancer agents which are particularly aggressive toward leukemia cells. The current method of synthesizing these compounds is lengthy, costly, and has a very low yield. Our group has developed a novel one-step method to produce precursors of these compounds. This procedure increases product yields, eliminates the use of toxic …

Secis Elements In The Coding Regions Of Selenoprotein Transcripts Are Functional In Higher Eukaryotes, Heiko Mix, Alexey V. Lobanov, Vadim Gladyshev

Vadim Gladyshev Publications

Expression of selenocysteine (Sec)-containing proteins requires the presence of a cis-acting mRNA structure, called selenocysteine insertion sequence (SECIS) element. In bacteria, this structure is located in the coding region immediately downstream of the Sec-encoding UGA codon, whereas in eukaryotes a completely different SECIS element has evolved in the 3’-untranslated region. Here, we report that SECIS elements in the coding regions of selenoprotein mRNAs support Sec insertion in higher eukaryotes. Comprehensive computational analysis of all available viral genomes revealed a SECIS element within the ORF of a naturally occurring selenoprotein homolog of glutathione peroxidase 4 in fowlpox virus. The fowlpox …

Determination Of Hydro-Morphological Effects Of The Rappahannock Shoal Channel On The District Of Columbia Nutrient Allocation Under The Chesapeake Bay Agreement, Harry V. Wang

Reports

No abstract provided.

Amyloid-Β-Induced Pathological Behaviors Are Suppressed By Ginkgo Biloba Extract Egb 761 And Ginkgolides In Transgenic Caenorhabditis Elegans, Yanjue Wu, Zhixin Wu, Peter Butko, Yves Christen, Mary P. Lambert, William L. Klein, Christopher D. Link, Yuan Luo

Faculty Publications

Amyloid-β (Aβ) toxicity has been postulated to initiate synaptic loss and subsequent neuronal degeneration seen in Alzheimer's disease (AD). We previously demonstrated that the standardized Ginkgo biloba extract EGb 761, commonly used to enhance memory and by AD patients for dementia, inhibits Aβ-induced apoptosis in neuroblastoma cells. In this study, we use EGb 761 and its single constituents to associate Aβ species with Aβ-induced pathological behaviors in a model organism, Caenorhabditis elegans. We report that EGb 761 and one of its components, ginkgolide A, alleviates Aβ-induced pathological behaviors, including paralysis, and reduces chemotaxis behavior and 5-HT hypersensitivity in a transgenic …