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2003

Genetics

Articles 1 - 9 of 9

Full-Text Articles in Life Sciences

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate Aug 2003

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate

Sheep Updates

This session covers eight papers from different authors:1. Biosecurity in the sheep industry will improve production and profits Ashley Mercy, Department of Agriculture, Western Australia 2. Easy care sheep David Scobie, AgResearch, PO Box 60 Lincoln, 8152, New Zealand 3. Targeting the market’s requirements - live sheep exports Neil Buchanan, Manager Animal Health Operations, Primary Industries & Resources, South Australia 4. Setting flock breeding objectives Kevin Bell, Sheep Management and Production Consultants, Kojonup WA 5. Future feeding of sheep in Western Australia John Milton and Graeme Martin, The University of Western Australia 6. Recipe for getting back into Sheep Bob …

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The Virulence Activator Apha Links Quorum Sensing To Pathogenesis And Physiology In Vibrio Cholerae By Repressing The Expression Of A Penicillin Amidase Gene On The Small Chromosome, Gabriela Kovacikova, Wei Lin, Karen Skorupski Aug 2003

The Virulence Activator Apha Links Quorum Sensing To Pathogenesis And Physiology In Vibrio Cholerae By Repressing The Expression Of A Penicillin Amidase Gene On The Small Chromosome, Gabriela Kovacikova, Wei Lin, Karen Skorupski

Dartmouth Scholarship

Activation of the tcpPH promoter on the Vibrio pathogenicity island by AphA and AphB initiates the Vibrio cholerae virulence cascade and is regulated by quorum sensing through the repressive action of HapR on aphA expression. To further understand how the chromosomally encoded AphA protein activates tcpPH expression, site-directed mutagenesis was used to identify the base pairs critical for AphA binding and transcriptional activation. This analysis revealed a region of partial dyad symmetry, TATGCA-N6-TNCNNA, that is important for both of these activities. Searching the V. cholerae genome for this binding site permitted the identification of a second one upstream of a …

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Crystal Structure Of The Sars Protein From Staphylococcus Aureus, Ronggui Li, Adhar C. Manna, Shaodong Dai, Ambrose L. Cheung, Gongyi Zhang Jul 2003

Crystal Structure Of The Sars Protein From Staphylococcus Aureus, Ronggui Li, Adhar C. Manna, Shaodong Dai, Ambrose L. Cheung, Gongyi Zhang

Dartmouth Scholarship

The expression of virulence determinants in Staphylococcus aureus is controlled by global regulatory loci (e.g., sarA and agr). One of these determinants, protein A (spa), is activated by sarS, which encodes a 250-residue DNA-binding protein. Genetic analysis indicated that the agr locus likely mediates spa repression by suppressing the transcription of sarS. Contrary to SarA and SarR, which require homodimer formation for proper function, SarS is unusual within the SarA protein family in that it contains two homologous halves, with each half sharing sequence similarity to SarA and SarR. Here we report the 2.2 Å …

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Alpha-Toxin Is Required For Biofilm Formation By Staphylococcus Aureus, Nicky C. Caiazza, George A. O'Toole May 2003

Alpha-Toxin Is Required For Biofilm Formation By Staphylococcus Aureus, Nicky C. Caiazza, George A. O'Toole

Dartmouth Scholarship

Staphylococcus aureus is a common pathogen associated with nosocomial infections. It can persist in clinical settings and gain increased resistance to antimicrobial agents through biofilm formation. We have found that alpha-toxin, a secreted, multimeric, hemolytic toxin encoded by the hla gene, plays an integral role in biofilm formation. The hla mutant was unable to fully colonize plastic surfaces under both static and flow conditions. Based on microscopy studies, we propose that alpha-hemolysin is required for cell-to-cell interactions during biofilm formation.

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Rpb4p, A Subunit Of Rna Polymerase Ii, Mediates Mrna Export During Stress, Marganit Farago, Tal Nahari, Christopher Hammel, Charles N. Cole, Mordechai Choder Feb 2003

Rpb4p, A Subunit Of Rna Polymerase Ii, Mediates Mrna Export During Stress, Marganit Farago, Tal Nahari, Christopher Hammel, Charles N. Cole, Mordechai Choder

Dartmouth Scholarship

Changes in gene expression represent a major mechanism by which cells respond to stress. We and other investigators have previously shown that the yeast RNA polymerase II subunit Rpb4p is required for transcription under various stress conditions, but not under optimal growth conditions. Here we show that, in addition to its role in transcription, Rpb4p is also required for mRNA export, but only when cells are exposed to stress conditions. The roles of Rpb4p in transcription and in mRNA export can be uncoupled genetically by specific mutations in Rpb4p. Both functions of Rpb4p are required to maintain cell viability during …

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2003 Nebraska Swine Report, Duane E. Reese Jan 2003

2003 Nebraska Swine Report, Duane E. Reese

University of Nebraska-Lincoln Extension: Historical Materials

This publication was prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching and Research programs. It deals with the results that were done in reproduction, breeding, health, genetics, nutrition, economics and housing of swine.

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Dna Sequence Of Melanocortin 1-Receptor Gene In Coturnix Japonica: Correlation With Three E Locus Alleles—E,E+, And Erh, Rupali Ugrankar, Kim Cheng, Ronald Okimoto Jan 2003

Dna Sequence Of Melanocortin 1-Receptor Gene In Coturnix Japonica: Correlation With Three E Locus Alleles—E,E+, And Erh, Rupali Ugrankar, Kim Cheng, Ronald Okimoto

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The melanocortin 1-receptor (MC1-R) gene plays a key role in the expression of fur and feather color in mammals and birds by regulating the distribution of two melanin pigments: eumelanin (black/brown) and pheomelanin (red/yellow). MC1-R corresponds to the classical Extension (E) locus in mice, pigs, dogs, horses, and chickens. Three E locus alleles, the wild-type (e+), brown (E), and redhead (erh) have been identified in Japanese quail (Coturnix japonica). To determine if the quail E locus phenotypes were due to variation in the MC1-R gene, the coding region of the MC1-R gene was PCR amplified and DNA sequenced using genomic …

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Studies On Rice Transformation And The Use Of Transformed Plants [Electronic Resource], Qiming Shao Jan 2003

Studies On Rice Transformation And The Use Of Transformed Plants [Electronic Resource], Qiming Shao

LSU Doctoral Dissertations

This research was conducted to enhance utilization of the Liberty herbicide resistance transgene in rice. Non-lethal methods to determine the sensitivity of transgenic rice plants to hygromycin B and Liberty were developed, tested and used in this research. Four homozygous transformed plants were selected to make reciprocal crosses with their non-transformed parent cultivars Taipei 309 and Nipponbare. Their resistances to Liberty and hygromycin B were controlled by the closely linked single dominant genes bar and hpt. Some non-resistant phenotypes in the F2 populations were due to gene silencing. The bar gene in some of these plants were allelic and some …

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Isolation And Characterisation Of A Pax Group Iii Gene From The Freshwater Crayfish Cherax Destructor: Implications For The Evolution Of Muscle Regeneration, Robert B. White Jan 2003

Isolation And Characterisation Of A Pax Group Iii Gene From The Freshwater Crayfish Cherax Destructor: Implications For The Evolution Of Muscle Regeneration, Robert B. White

Theses : Honours

Pax genes encode transcription factors that are highly evolutionarily conserved and are vital for animal development. Vertebrate Pax group III genes Pax3 and Pax7 are required for proper development of muscular and central nervous systems. In their roles in muscular systems, Pax3 and Pax7 specify myoblasts (muscle progenitor cells). Pax3 is predominantly involved in embryonic myogenesis and specifies embryonic myoblasts. In contrast, Pax7 specifies adult myoblasts (muscle satellite cells), and has been demonstrated to be vital for adult muscle regeneration. Recent evidence also implicates a role for Pax7 in the conversion of certain stem cells to the myogenic lineage. The …

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