Life Sciences Commons^™

The Effects Of Coelomic Fluid And Isotonic Homogenates Of The Freshwater Bryozoan Lophopodella Carteri On Fish Tissue, Roger H. Meacham

Master's Theses

A study was made to determine if fish tissue was poisoned by the coelomic fluid extracted from the common coelomic cavity of colonies of the freshwater bryozoan, Lophopodella carteri (Hyatt). Additional studies were made of fish tissues, in vivo and in vitro, when exposed to homogenates of crushed colonies of L. carteri in isotonic medium.

The fish exposed to the coelomic fluid did not show any signs of respiratory distress and therefore the poisonous quality must be located elsewhere in the bryozoan. The homogenates were determined to be poisonous as fishes exposed to a 3.0% solution were killed within an …

The Ultrastructure Of The Vibratory Muscle Of Crotalus Horridus, Louis Pastore

Master's Theses

A comparison of the vibratory and epaxial muscles of Crotalus horridus horridus Linnaeus wa smade. muscle fibers, fibrils, filaments, mitochondria and sarcoplasmic reticulum were described in this study. The sarcomeres of the epaxial muscle were almost three times the length of those of the vibratory muscle. The A band was observed to be about twice the length of the I band in the vibratory muscle. As seen in cross section the vibratory muscle had fibrils arranged in discrete packets with a limited number of thick myofilaments. In contrast, the fibrils of epaxial muscle were less conspicuously separated from each other …

A Comparative Study Of The Sulfhydryl Groups In The Gastrocnemius Of The Normal And X-Irradiated Pet Mouse, Robert Edward Shervette

Master's Theses

It has been recognized that hyperpigmentation of the skin is the result of eliminating the. -SH inhibition when treatment is by x-irraidiation. The results of a. study by fieams and Schaeffer indicate : a progressive increase of melanocytes in the skin with an increasing dosage of x-irradiation. However, it was also noted that the effect of x-irradiatin on the gastrocnemius of the PET mouse resulted in a decrease in the number of intramuscular melanocytes. The present study was designed to determine whether the changes in the x-irradiated muscle is related to possible changes in -SH concentration.The technique employed is a …

Respiratory Activity Of Vibratory, Tail Epaxial And Midbody Expaxial Muscle In Crotalus Horridus, Aqkistrodon Contortrix, And Thamophis Sirtalis., James E. Forbes

Master's Theses

The purpose of this study was to determine if the muscles associated with tail vibration in snakes are physiologically specialized for vibration. Timber rattle­ snakes (Crotalus horridus) , copperheads (Agkistrodon contortrix) and garter snakes (Thamnophis sirtalis) were used. The copperhead represented a species which vibrates its tail but has no vibratory apparatus comparable to that of the rattlesnake. The garter snake does not vibrate its tail. A comparison of respiratory activities was made between the vibratory and tail epaxial muscle and the mid­ body epaxial muscle within and between the three species. Oxygen consumption of teased muscle fibers and muscle …

The Comparative Osteology Of The Trunk Skeletons Of Three Species Of Paralichthys, Family Bothidae, From North Carolina, William Marion Hall

Master's Theses

Paralichthys albigutta Jordan and Gilbert, the gulf flounder, which occurs over sand bottoms, and Paralichthys let:nostigma Jordan and Gilbert, the southern flounder,which occurs over mud bottoms , are southern species with ranges extending into the Gulf of Mexico.Paralichthys dentatus, the summer flounder, is tho northern species and is tolerant of several types of bottoms but is most frequently found over sand or hard bottoms. The ranges of the three species overlap in an area along the eastern coast of the United States from central North Carolina to near Jacksonville, Florida. The flounders are closely related and unless the pig­ment pattern …

Electrical Response Of Frog Skin Epidermis To Sodium Ions, James H. Martin

Master's Theses

Presently, the theory is held that the total frog skin potential (P.D.) is generated within the epidermis at two borders, the "outer border" and the "inner border, " which are said to be specifically permeable to Na⁺ and K⁺, respec­tively. This thesis concerns itself only with the electrical response of the "outer border" to varying Na⁺ concentrations in the solutions at the epidermis, [Na⁺]_o. Contrary to expectation from the Nernst equation, the P.D. changes by only 17 to 35 mV, instead of theoretically 58 mV upon a 10 fold change in [Na …

A Study Of Hybridization And Behavioral Characters In Percina Notogramma (Rany And Hubbs) And Percina Peltata (Stauffer), Family Percidae, Jules J. Loos

Master's Theses

In view of the close phylogenetic relationship between the two sym­patric species of darters, Percina notogramma (Raney and Hubbs) and Percina peltata (Stauffer), placed in the subgenus Alvordius by Bailey and Gosline (1955), it is of interest to compare them in more detail than has been done in the past. Percina notograrnma occurs only in the Chesapeake Drainage in Virginia, Maryland and West Virginia whereas P. peltata ranges from Virginia to New York. Adequate comparisons of their external. morphological characters have been presented by Raney and Hubbs (1948) in their original description of P. notogramma. Supplementary data were provided by …

The Effects Of A Nerve Growth-Promoting Protein On The Integument Of Pet Mice, Craig Bauman Satterlee

Master's Theses

A factor which specifically stimulates the growth of sensory and sympathetic nerve cells has been under investigation for a number of years. This factor, originally isolated in 1951, from mouse sarcomas 37 and 180, was named the "nerve growth factor". Its action was first shown in vivo by inoculating the sarcoma into chick embryos and subsequently in vitro by culturing chick ganglia and mouse sarcoma together. In the years immediately following the initial discovery, much more potent sources of NGF were found in the salivary glands and salivary secretions of a variety of species. These include rattle snake venom, and …

A Comparative Study Of The Parasite Of Certain Salientia From Pocahontas State Park, Virginia, Ronald Arthur Campbell

Master's Theses

Four species of salientia representing three selected habitats were collected from May 5th through October 4th, 1966 and examined for parasites. Of the 116 host specimens, 30 were Rana catesbeiana Shaw; 29 clamitans Latreille; 29 Bufo fowleri Hinckley; 28 Hyla versi­color (Le Conte). A total of 34 species of parasites was recovered: Protozoa, 10; Trematoda, 9 ; Cestoda, 2; Nematoda, 11; Acanthocephala, 1; and Acarina, 1.

Although a relatively small number of hosts was examined , the results generally agree with those found in more extensive investigations of similar type. The aquatic hosts, R. catesbeiana and R. clamitans, harbored the …

A Metabolic Study Of The Isolated Perfused Snake Liver, James E. Turner

Master's Theses

A metabolic study was made on the isolated, perfused liver of Natrix fasciata fasciata (Linnaeus, 1776). Uric acid production, perfusate protein levels, serum protein patterns, perfusate glucose levels, and bile production were evaluated in this study as criteria of liver function.

The perfused liver was found to produce uric acid up through the fourth hour of perfusion. No significant differences (between the perfusions) were found in uric acid production/ gm of liver tissue.

Perfusate protein and glucose levels were found to be of a fluctuating or cyclic nature. There was found to be an inverse relationship between these two levels: …

Interference Of Ultraviolet Inactivated Parainfluenza 3 And Echo 21 Viruses With The Active Viruses In Tissue Culture, William Ernest Steinmetz

Master's Theses

Experiments were conducted to investigate the possibility of the production of interference by ultraviolet inactivated (UVI) Parainfluenza 3 (Para 3) and Echo 21 viruses with active Para 3 and Echo 21 viruses in tissue culture (TC).

Four major experiments were conducted: the effect of UVI Para ~ on active Para 3 in rhesus monkey kidney cell (MKC) tissue culture; the effect of UVI Para 3 on active Para 3 in HEp-2 TC; the effect of UVI Para 3 on Echo 21 in MKC TC; and the effect of UV! Echo 21 on Echo 21 in MKC TC. The rates of …