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Articles 1 - 30 of 1281
Full-Text Articles in Life Sciences
C. Compactum Acts As A Comprehensive Climate Archive And Ecological Foundation In The Labrador Sea, Sadie Heckman
C. Compactum Acts As A Comprehensive Climate Archive And Ecological Foundation In The Labrador Sea, Sadie Heckman
CMC Senior Theses
Clathromorphum compactum, a species of crustose coralline algae (CCA), is incredibly valuable for the future of high latitude ocean health, both as a comprehensive archive of changing ocean conditions, and ecologically as a foundational species for promoting biodiversity. Previous work establishes C. compactum as an effective climate proxy, and its life history provides several advantages for this use. C. compactum grow in nongeniculate, generally radial formations on hard substrates, over a wide distribution in mid-to-high latitude oceans and at subtidal depth ranges. Indeterminate growth leads to extreme longevity in C. compactum (Halfar et al., 2008), and growth rates are relatively …
Biophysical Factors Affecting Habitat Suitability For Crassostrea Virginica, Jason D. Tilley
Biophysical Factors Affecting Habitat Suitability For Crassostrea Virginica, Jason D. Tilley
Dissertations
Oyster reefs provide a variety of important ecosystem services. However, the mortality rate of eastern oyster, Crassostrea virginica, the dominant species that produces oyster reefs in the northern Gulf of Mexico, is increasing at an alarming rate due to a variety of abiotic and biological factors. I examined how biophysical factors, including the less-studied fatty acid profiles of the suspended particulate matter on which oysters feed, influenced morphometric condition of C. virginica.
I sampled suspended particulate matter (SPM) and oysters in-situ in the western Mississippi Sound, which historically supported the majority of oyster production in Mississippi waters. Sampling …
The Relationship Between Biofouling Adhesion And Frequency Of Mechanical Cleaning To Control The Recruitment To Clear Coatings, Dylan Thomas Eggers
The Relationship Between Biofouling Adhesion And Frequency Of Mechanical Cleaning To Control The Recruitment To Clear Coatings, Dylan Thomas Eggers
Theses and Dissertations
There is a need to control biofouling on sensors and transparent windows in the marine environment. Clear silicone coatings offer a method to reduce the adhesion strength of marine organisms to the surface, however, these also require mechanical cleaning to maintain the surfaces free of fouling. This research evaluated the performance of eighty seven formulations and took the top two candidates to investigate a) the frequencies of wiping required by a brush to maintain two coatings and uncoated glass surfaces free of fouling and b) the effects of clear silicone coatings fouling release properties on required frequencies. The panels were …
Patterns In Winter Stonefly Distribution Along A River Continuum And Land-Use Gradient In Northwest Arkansas Streams, Zachary Tipton
Patterns In Winter Stonefly Distribution Along A River Continuum And Land-Use Gradient In Northwest Arkansas Streams, Zachary Tipton
Graduate Theses and Dissertations
Freshwater ecosystems are facing a crisis with extinction rates of aquatic species exceeding those of their terrestrial counterparts by up to fivefold. This decline is predominantly attributed to evolving land use patterns within watersheds, leading to chemical and physical transformations in freshwater habitats. Northwest Arkansas (NWA) represents one of the fastest-growing regions in the United States, undergoing substantial shifts in land use. Consequently, the status of aquatic life in this region remains uncertain. Addressing this concern, the latest Arkansas Wildlife Action Plan emphasizes the necessity of distribution and population data to guide conservation efforts for Species of Greatest Conservation Need …
Interactions Between Sediment Mechanical Structure And Infaunal Community Structure Following Physical Disturbance, William Cyrus Roger Clemo
Interactions Between Sediment Mechanical Structure And Infaunal Community Structure Following Physical Disturbance, William Cyrus Roger Clemo
<strong> Theses and Dissertations </strong>
Shallow, river-influenced coastal sediments are important for global carbon storage and nutrient cycling and provide a habitat for diverse communities of invertebrates (infauna). Elevated bed shear stress from extreme storms can resuspend, transport, and deposit sediments, disrupting the cohesive structure of muds, and sorting and depositing sand eroded from beaches. These physical disruptions can also resuspend or smother infauna, decreasing abundances and changing community structure. Infaunal activities such as burrowing, tube construction, and feeding can impact sediment structure and stability. However, little is known about how physical disturbance impacts short and long-term sediment habitat suitability and whether disturbance-tolerant infauna influence …
Ecology And Management Of Dyer's Woad (Isatis Tinctoria) In Northern Utah, Erin Marie Hettinger
Ecology And Management Of Dyer's Woad (Isatis Tinctoria) In Northern Utah, Erin Marie Hettinger
All Graduate Theses and Dissertations, Fall 2023 to Present
Dyer’s woad (Isatis tinctoria L.) is a non-native forb that continues to threaten natural areas throughout Northern Utah and much of the Intermountain West. Once introduced, dyer’s woad can become extremely invasive, decreasing forage quality, and displacing native species. While dyer’s woad is found throughout much of Northern Utah, its range in other states remains limited. If promptly managed, control success in these areas will be much higher and populations may be kept at bay before ecological damage becomes severe.
This project tested the ability of dyer’s woad seedlings to compete with common rangeland grasslands at varied densities as …
Reducing Inputs And Adding Value To Turfgrass Systems Through Clover Inclusion And Plant Growth-Promoting Rhizobacteria Applications, Paige E. Boyle
Reducing Inputs And Adding Value To Turfgrass Systems Through Clover Inclusion And Plant Growth-Promoting Rhizobacteria Applications, Paige E. Boyle
All Graduate Theses and Dissertations, Fall 2023 to Present
Recently, the US has seen an expansion in the amount of turfgrass land cover (lawns, parks, roadsides, sports fields, and golf courses), as well as an interest in reducing fertilizer, water, and pesticide use in these grass systems. To help maintain quality and function while reducing resource inputs, two promising approaches have emerged: planting clover into lawns and applying plant growth-promoting rhizobacteria.
White clover and grass mixtures have been studied for their ability to cut down on fertilizer usage and provide a uniform, dark green lawn, but other clover types have not been as widely studied and may provide similar …
Using Biometrics, Behavioral Observations, And Multiple Molecular Techniques To Assess The Impacts Of Changes In Temperature And Salinity On The Common Bay Mussel (Mytilus Trossulus), Casey Martin
Dissertations and Theses
The intertidal zone is a place of rapid and frequent change that is home to a variety of creatures who are essential to the integrity of the habitat. Mussels are robust sessile bivalves that anchor to the rocks of the intertidal. The prominent species on the Oregon Coast, the Common Bay Mussel (Mytilus trossulus), plays an essential role as a coastal food source, water column filter, and barrier to prevent erosion due to wave action. Mytilus trossulus withstands daily shifts in temperature, salinity, and tide, as well as seasonal changes. Global climate change due to excess carbon emissions …
Do Microplastics, Vibrio Bacteria, And Warming Water Temperatures Cause Disease In The Northern Star Coral, Astrangia Poculata?, Heather Leigh Sheffey
Do Microplastics, Vibrio Bacteria, And Warming Water Temperatures Cause Disease In The Northern Star Coral, Astrangia Poculata?, Heather Leigh Sheffey
OES Theses and Dissertations
As marine plastic pollution increases, it becomes imperative to study the effects of microplastics on marine fauna. Marine plastic debris is a vector for disease to marine invertebrates (Lamb et al., 2018; Rotjan et al., 2019). In this context, studies have shown Astrangia poculata will consume microplastics as they do their normal food (Allen et al, 2017; Rotjan et al., 2019). Further, with temperatures increasing worldwide, there is a concern the virulence of disease-causing bacteria will increase (Vezzulli et al., 2015). In a series of manipulative laboratory experiments, this study quantified changes in respiration rates and visual health of Astrangia …
Collaborative And Engaged Research To Strengthen Equity And Adaptive Governance In Co-Managed Fisheries, Gabrielle V. Hillyer
Collaborative And Engaged Research To Strengthen Equity And Adaptive Governance In Co-Managed Fisheries, Gabrielle V. Hillyer
Electronic Theses and Dissertations
Small-scale, co-managed fisheries are found throughout the world and often represent intertwining cultures, societies, communities, economies, institutions, and governments. They face complex issues, derived from ecological and social sources. Solving these issues requires diverse expertise, often developed through engaged methodologies which can facilitate collaborative solution creation between researchers, community members, and others. In this dissertation, I demonstrate the benefits of these engaged methodologies and review how they, when coupled with anticolonial approaches to research, can create more equitable solutions to complex issues. This dissertation focuses on multiple projects within the wild clam fishery in Maine including: (1) the creation of …
Tidal Flooding In The Mid-Atlantic Region Of The Us: Water Quality Effects In The Lower Chesapeake Bay, Alfonso Macias Tapia
Tidal Flooding In The Mid-Atlantic Region Of The Us: Water Quality Effects In The Lower Chesapeake Bay, Alfonso Macias Tapia
OES Theses and Dissertations
Many coastal areas around the globe suffer from nutrient pollution and its environmental, social, and economic consequences. Nutrient inputs can come from point (e.g., the end of a pipe) and nonpoint sources, from which the former are better constrained as sampling need only be conducted at a discharge point. Given the temporal and spatially extensive nature of tidal flooding events, they can represent another type of nonpoint source of nutrients to adjacent water bodies heretofore, unexamined and quantified. Most studies examining impacts of tidal flooding have focused on threats to resources on land, such as urban infrastructure and human health …
Unearthing Complexity: Tangible Histories Of Water And Earth, Alexis Violet
Unearthing Complexity: Tangible Histories Of Water And Earth, Alexis Violet
Masters Theses
Unearthing Complexity investigates conceptions of time and surface through geological stories of the water and earth. Building on theories of deep time, hydrofeminism, critical zones, and grounding, I hope to foster a deeper awareness of time scales other than our own and a more tangible understanding of the embodied experience of matter in the universe. Working toward a new literacy of the water and earth in which they are recognized as living, changing bodies to which we are inherently tied at a molecular level, the site of this multiscalar inquiry occurs in the coastal zones of the Narragansett Bay where …
Fecal Pellet Production By North Atlantic Zooplankton, Michael Gibson
Fecal Pellet Production By North Atlantic Zooplankton, Michael Gibson
Undergraduate Honors Theses
Fecal pellet carbon (FPC) production by zooplankton is a significant component of the ocean’s biological carbon pump: the suite of biological processes that mediate export of carbon to the deep ocean, ultimately leading to the sequestration of atmospheric carbon dioxide in the ocean. In this study, mesozooplankton (zooplankton 0.2 mm to ~2 cm) were collected from the epipelagic zone in the temperate North Atlantic Ocean during day and night in May 2021. Zooplankton were live separated into five size fractions and incubated on board ship in natural surface seawater to measure fecal pellet production rate of the mixed mesozooplankton community. …
Effect Of Elevated Temperature And Roundup Exposure On Oxidative Stress And Antioxidant Status In The American Oyster, Afsana Chowdhury
Effect Of Elevated Temperature And Roundup Exposure On Oxidative Stress And Antioxidant Status In The American Oyster, Afsana Chowdhury
Theses and Dissertations
Aquatic organisms are frequently exposed to various environmental stressors. In this study, we observed the short-term (1-week) combined dose-dependent effect of Roundup, a glyphosate-based herbicide, and high temperature (30°C) on the morphology of gills and digestive glands, expression of heat shock protein-70 (HSP70), dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), antioxidant enzymes such as catalase (CAT) and superoxidase dismutase (SOD) in tissues of American oyster (Crassostrea virginica). Histological analyses showed an increase in mucous production in the gills and digestive glands, hemocyte aggregation in the connective tissues as well as structural change of the lumen in the digestive glands …
Evapotranspiration And Energy Balance Of Irrigated Urban Turfgrass, Matthew D. Miksch
Evapotranspiration And Energy Balance Of Irrigated Urban Turfgrass, Matthew D. Miksch
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Water usage for irrigation is a big consumer of water resources in urban areas in Utah and other parts of the Intermountain Region of the Western United States. As populations continue to increase in these states, it is important to understand how much water is being used by urban landscapes in order to plan and manage future water resources. Evapotranspiration (ET), or the amount of water leaving a surface over a certain timeframe due to both transpiration from plants and evaporation from the soil, is a key variable in understanding how much water urban landscapes are really using to grow …
An Overview Of Serial Depletions Of Global Marine Fisheries 1950 To 2019, Alison Follmer
An Overview Of Serial Depletions Of Global Marine Fisheries 1950 To 2019, Alison Follmer
Biological Sciences Undergraduate Honors Theses
Overfishing is a global issue that poses a significant risk to the entire ocean ecosystem in diminishing biodiversity and ecosystem function. This thesis examined the pattern and pace of fisheries depletions due to commercial fishing during the past 70 years. The Food and Agriculture Organization of the United Nations (FAO) Division of Fisheries and Aquaculture maintains a database of global hauls of marine taxa (reported in metric tonnes) from 1950 – 2019. These data were queried to determine the total number and sequence of fisheries depletions documented by the historic record. Analysis of this database showed progressive, linearly-increasing exploitation of …
Geomorphology Of Tidal Wetlands: Impacts Of Extreme And Annual Flood Events To Salt Marsh And Mangrove Systems, Frances R. Griswold
Geomorphology Of Tidal Wetlands: Impacts Of Extreme And Annual Flood Events To Salt Marsh And Mangrove Systems, Frances R. Griswold
Doctoral Dissertations
Tidal wetlands are vital for buffering coastal settings from the threats of accelerated sea level rise and storms. Understanding the factors that are most influential for the maintenance and recovery of tidal wetlands after extreme events compounded by future accelerated sea level rise is of the utmost importance, yet this knowledge is not well established. Two tidal wetland schemas investigated in this dissertation are mangrove systems in Vieques, Puerto Rico (including robust lagoonal-mangrove forest systems and fringing mangrove forests), and salt marshes in New England. While the climatic forcings, vegetation type, and locations are vastly different for these two tidal …
Movement, Behavior, And Trophic Ecology Of A Pelagic Predator Guild In The Eastern Tropical Pacific Ocean, Ryan Keith Logan
Movement, Behavior, And Trophic Ecology Of A Pelagic Predator Guild In The Eastern Tropical Pacific Ocean, Ryan Keith Logan
All HCAS Student Capstones, Theses, and Dissertations
Pelagic apex predators exert strong influences on ecological communities, and often support valuable commercial or recreational fisheries worldwide. Yet, due to their rarity and pelagic lifestyle, many species, such as billfishes, have proven particularly difficult to study at resolutions necessary to define dynamics of recovery from fishery interaction, physical interaction with environmental features and prey exploitation, and competitive interactions among other billfish predators. This leads to a paucity of knowledge on billfish ecology and habitat use, and hinders management efforts. With the ever-improving and miniaturization of technology and oceanographic datasets, the ability to define and quantify these interactions of fish …
What Drives Larval Condition For Northern Anchovy (Engraulis Mordax)? Implications For Coastal Pelagic Species Recruitment Fluctuations And Fishery Management Practices, Michelle Robidas
Theses
Discerning the causes of population boom and bust cycles for coastal pelagic species (CPS) has been a major focus of fisheries management research for over a century. Year-class strength is contingent on larval survival and condition, which can be influenced by larval size at age and growth rate. These two factors, in turn, can be affected by maternal investment and environmental variables such as water temperature, salinity, oxygen, and chlorophyll a. We evaluated each of these factors from 127 two to eight week-old Northern Anchovy (Engraulis mordax) larvae off the coast of San Diego to San Francisco, …
Quantifying The Carbon Stored And Sequestered By The Trees On Pomona College’S Campus, Paola A. Giron-Carson
Quantifying The Carbon Stored And Sequestered By The Trees On Pomona College’S Campus, Paola A. Giron-Carson
Scripps Senior Theses
We are experiencing a climate crisis that must be confronted with strategic mitigation. Pomona College contributes to the climate crisis through its emissions for which there is a baseline record. However there is no baseline record of the climate mitigation currently performed by the trees on Pomona’s campus through carbon storage. This study seeks to determine a current baseline quantity of carbon stored and sequestrated by Pomona’s trees as well as possible courses of climate mitigation for Pomona College to take. Initial information gathering was conducted through interviews with several stakeholders. This study was conducted using data collected prior to …
Food Waste Storage Gaseous Emissions Detection And Quantification Using Infrared Spectroscopy, Ryley A. Burton-Tauzer
Food Waste Storage Gaseous Emissions Detection And Quantification Using Infrared Spectroscopy, Ryley A. Burton-Tauzer
Cal Poly Humboldt theses and projects
A growing interest in sustainable waste management and the implementation of new policies have prompted a shift towards alternative resource recovery methods for organic waste, including food waste. To effectively assess alternative food waste treatment scenarios, it is important to evaluate the life cycle impacts associated with each scenario. The storage phase of food waste, encompassing its accumulation in kitchens, and storage in bins for collection and transportation, has been overlooked as a source of greenhouse gases in previous studies. This investigation aimed to identify the greenhouse gases emitted during the initial five-day period of low-oxygen storage. An open dynamic …
Impacts And Uncertainties Of Climate Change On The Chesapeake Bay, Kyle E. Hinson
Impacts And Uncertainties Of Climate Change On The Chesapeake Bay, Kyle E. Hinson
Dissertations, Theses, and Masters Projects
Climate change impacts in the Chesapeake Bay will limit the efficacy of nutrient reduction efforts and decrease dissolved oxygen, but uncertainties associated with the magnitude of these effects remain. An understanding of underlying mechanisms that have driven recent warming trends will narrow uncertainties for future pathways of temperature change. Additionally, future simulations of climate impacts in the estuary are dependent on multiple different sources of uncertainty, many of which have not yet been fully evaluated. This dissertation used a three- dimensional coupled hydrodynamic-biogeochemical model to investigate recent warming trends as well as underlying uncertainties likely to influence regional projections of …
Timing Of Diversification, Dispersal, And Biogeography Of Parrots In The Genus Amazona (Psittaciformes: Psittacidae) Throughout The Caribbean, Visualized In Gis, Christopher Kingwill
Timing Of Diversification, Dispersal, And Biogeography Of Parrots In The Genus Amazona (Psittaciformes: Psittacidae) Throughout The Caribbean, Visualized In Gis, Christopher Kingwill
Master's Theses
Avian fossil records from across the Caribbean (Greater and Lesser Antilles) demonstrate higher avian diversity prior to extinction events due to climate change at the end of the Pleistocene and human impact across the Caribbean throughout the Holocene. Amazon parrots (Amazona) are a diverse genus of New World parrots found throughout Central and South America, as well as the Caribbean. Their phylogeny and evolutionary history, specifically for Caribbean species, has been debated in terms of source areas in Central and South America and the timing of and number of colonization events to different islands that preceded diversification into …
Remote Sensing Of Georgia Tidal Marsh Habitats Using Aerial Photography And Planetscope Satellite Imagery, Harrison M. Currin
Remote Sensing Of Georgia Tidal Marsh Habitats Using Aerial Photography And Planetscope Satellite Imagery, Harrison M. Currin
Electronic Theses and Dissertations
Globally, tidal marshes cover about 90,800 km. Within the state of Georgia tidal marshes are primarily located behind the barrier islands and total 1,619 km2. The combination of high salinity environments and daily inundation, and being dependent on river output, make these dynamic systems. Tidal marshes provide numerous ecosystem services such as carbon and nitrogen sequestration, flood control, coastal protection, and numerous biogeochemical processes. Due to their unique position, tidal marshes are under threat from sea level rise, drought, coastal development, and large-scale disturbance events. Tidal freshwater marshes are especially susceptible to these threats due to their geographic …
Microplastic Accumulation In The Digestive Tract Of Young-Of-Year Atlantic Sharpnose Sharks (Rhizoprionodon Terraenovae) In The Grand Strand, Sc, Andrew Curtis Sitlinger
Microplastic Accumulation In The Digestive Tract Of Young-Of-Year Atlantic Sharpnose Sharks (Rhizoprionodon Terraenovae) In The Grand Strand, Sc, Andrew Curtis Sitlinger
Electronic Theses and Dissertations
This study focused on the presence and accumulation of microplastic fibers in the digestive tract and livers of young-of-year Atlantic Sharpnose Sharks (Rhizopriondon terraenovae) from two sampling locations along the Grand Strand of South Carolina. R. terraenovae is a small, mesopredatory elasmobranch found abundantly along northwestern Atlantic Ocean coastlines. Thirty specimens of R. terraenovae were collected from May through August of 2020. Microplastics were found in all specimens. A total of 672 plastic particles were identified over the course of the study, with an average of 22.4 ± 10.5 (SD) plastics per specimen. The majority of the plastics were classified …
Larval Ecology Of Atlantic Bluefin Tuna (Thunnus Thynnus): New Insights From Otolith Microstructure, Biotic, And Abiotic Analyses From The Gulf Of Mexico And Mediterranean Sea, Estrella Malca
All HCAS Student Capstones, Theses, and Dissertations
Atlantic bluefin tuna (ABT), Thunnus thynnus, spawn in the Gulf of Mexico (GoM) and the Mediterranean Sea (MED). Spawning occurs within narrow temporal and environmental parameters. Efforts to characterize growth of ABT in wild conditions revealed a wide range of growth variability during the early life stages. This series of studies examined potential biotic and abiotic influences of larval growth from seven ABT cohorts, and identified several key drivers of growth for this commercially valuable species. A detailed investigation of larval dynamics using otolith microstructure was conducted as follows. First, companion growth curves and stable isotope analysis from the same …
Green On The Map - The Influence Of Conservation Easements On The Naturalness Of Landscapes In The United States, Nakisha Fouch
Green On The Map - The Influence Of Conservation Easements On The Naturalness Of Landscapes In The United States, Nakisha Fouch
All Dissertations
Large protected areas have long been the cornerstone of conservation biology, however, in an era branded by the human dominance of ecosystems, regional landscape structure and function are often a consequence of accumulated land-use decisions that may or may not include a nod to conservation planning. With underrepresentation of habitats in publicly protected areas, attention has focused on the function of alternative land conservation mechanisms. Private conservation easements (CEs) have proliferated in the United States, yet assessing landscape-level function is confounded by holder and donor intent, national and regional policy, regional landscape contexts, varying extents, resolution, and temporal scale. Over …
Weather Parameters Influencing The Incidence Of Citrus Canker Caused By Aw Strain In The Rio Grande Valley, Amit Sharma
Weather Parameters Influencing The Incidence Of Citrus Canker Caused By Aw Strain In The Rio Grande Valley, Amit Sharma
Theses and Dissertations
Citrus canker caused by bacterium Xanthomonas citri subsp. citri (Xcc) seriously affects the citrus industry by making the fruit unmarketable due to unsightly lesions on the fruit. Canker caused by Aw strain of Xcc was reported in the citrus trees located in the residential areas of the Rio Grande Valley (RGV). Canker severity differs amongst cultivars/varieties, and it is influenced by prevailing environmental conditions. Multiple regression modeling of the disease incidence with the environmental variables such as temperature, humidity, windspeed, wind gust, and rainfall was performed to understand the environmental conditions that are favorable for spread of citrus …
Quantifying Spatial Heterogeneity Of Wild Blueberries And Crop Water Stress Monitoring Using Remote Sensing Technologies, Kallol Barai
Quantifying Spatial Heterogeneity Of Wild Blueberries And Crop Water Stress Monitoring Using Remote Sensing Technologies, Kallol Barai
Electronic Theses and Dissertations
The wild blueberry is one of the major crops of Maine, with significant economic value and potential health benefits. Due to global climate change, drought impacts have been increasing significantly in recent years in the northeast region of the USA, causing significant economic losses in the agricultural sectors. It has been predicted to increase further in the future. Changing patterns of the elevated atmospheric temperatures, increased rainfall variabilities, and more frequent drought events have made the wild blueberry industry of Maine vulnerable, suggesting the adoption of novel approaches to mitigate the negative impacts of global climate changes. Also, wild blueberry …
Remotely Sensed Assessment Of The Preferred Habitat Of Alexandrium Catenella In The Gulf Of Maine And The Bay Of Fundy, Andre F. Bucci
Remotely Sensed Assessment Of The Preferred Habitat Of Alexandrium Catenella In The Gulf Of Maine And The Bay Of Fundy, Andre F. Bucci
Electronic Theses and Dissertations
Harmful Algal Blooms (HABs) of the toxic dinoflagellate Alexandrium catenella are an annually recurring problem in the Gulf of Maine (GoM), resulting in risks to human health and substantial economic losses due to shellfish harvesting closures. The monitoring approaches in the region are restricted to real-time identification of the HABs events, when they are clearly underway and already causing deleterious effects to the environment. To fully function as an early warning system rather than an immediate response, monitoring strategies need to be focused on environmental conditions preceding A. catenella HABs. However, the current understanding of the preferred habitat for A. …