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Osmotic Tolerance And Volume Regulation In In Vitro Cultures Of The Oyster Pathogen Perkinsus Marinus, Cl O'Farrell, Jf La Peyre, Kt Paynter, Em Burreson
Osmotic Tolerance And Volume Regulation In In Vitro Cultures Of The Oyster Pathogen Perkinsus Marinus, Cl O'Farrell, Jf La Peyre, Kt Paynter, Em Burreson
VIMS Articles
Growth rate. cell size, osmotic tolerance, and volume regulation were examined in cells of Perkinsus marinus cultured in media of osmolalities ranging from 168 to 737 mOsm (6.5-27.0 ppt). Cells cultured at the low osmolalities of 168 and 256 mOsm (6.5 and 9.7 ppt) began log phase growth 4 days postsubculture, whereas cells cultured at the higher osmolalities 341, 433, and 737 mOsm (12.7. 16.0, and 27.0 ppt) began log phase growth 2 days postsubculture. During log phase growth, cells from the higher osmolalities 341, 433, and 737 mOsm had shorter doubling times than cells from the lower osmolalities 168 …