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Perkinsus Marinus; Subtilisin; Serine Protease; Real-Time Pcr; Crassostrea Virginica; Crassostrea Gigas; Crassostrea Ariakensis; Crassotrea
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Full-Text Articles in Life Sciences
Effect Of Homogenate From Different Oyster Species On Perkinsus Marinus Proliferation And Subtilisin Gene Transcription, Gd Brown, Sl Kaattari, Kimberly S. Reece
Effect Of Homogenate From Different Oyster Species On Perkinsus Marinus Proliferation And Subtilisin Gene Transcription, Gd Brown, Sl Kaattari, Kimberly S. Reece
VIMS Articles
The modulation of Perkinsus marinus proliferation and subtilisin gene transcription by host (oyster) tissue was examined. Perkinsus rnarinus cells were cultured for 4 weeks in media supplemented with extract from either one of four different Crassostrea virginica stocks or with extract from one of two other Crassostrea species, C. ariakensis and C. gigas. After 4 weeks in culture, we determined cell counts and relative subtilisin gene transcription levels using quantitative real-time polymerise chain reaction (qRTPCR). Cell proliferation and subtilisin gene transcription were significantly lower when P. marinus' cells were grown in the presence of homogenate from any of the three …