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A Comparison Of Paraffin Histology And Hemolymph Analysis For The Diagnosis Of Haplosporidium Nelsoni (Msx) In Crassostrea Virginica (Gmelin), Eugene M. Burreson, M.Elizabeth Robinson, Antonio Villalba
A Comparison Of Paraffin Histology And Hemolymph Analysis For The Diagnosis Of Haplosporidium Nelsoni (Msx) In Crassostrea Virginica (Gmelin), Eugene M. Burreson, M.Elizabeth Robinson, Antonio Villalba
VIMS Articles
Diagnosis of the oyster pathogen Haplosporidium nelsoni (MSX) by paraffin histology is compared with a technique in which hemolymph drawn from the oyster adductor muscle sinus is examined for parasite plasmodia. Oysters from seed beds of the James River, Virginia imported to an MSX endemic area in May, 1986 were sampled monthly through December, 1986 and in February, 1987. A sample of 25 oysters was bled each month and then processed for sectioning. Of the 200 oysters sampled, 89 (44.5%) were diagnosed as infected using histology and 61 (30.5%) were diagnosed as infected using hemolymph examination. All the heavy and …
Chemotactic Response Of Fish Macrophages To Legionella-Pneumophila - Correlation With Pathogenicity, Ba Weeks, Sr Sommer, Hp Dalton
Chemotactic Response Of Fish Macrophages To Legionella-Pneumophila - Correlation With Pathogenicity, Ba Weeks, Sr Sommer, Hp Dalton
VIMS Articles
Chemotaxis is the directional migration of cells in response to a chemical stimulus. This phenomenon appears to be responsible for the accumulation of macrophages during inflammation This work represents an attempt to understand certain aspects of host-parasite relationships in Legionnaire's Disease. In the first phase of this study, we measured the chemotactic stimulation of fish macrophages by 2 strains of Legionella pneumophila, one virulent and one avirulent for guinea pigs. Results from this part of the study, coupled with the possibility that reduced chemotaxis may be a factor contributing to increased virulence, led us to initiate the second phase of …
Development And Evaluation Of Techniques To Study Acquired Immunity To Perkinsus Marinus In The Oyster, Crassostrea Virginica (Gmelin), Fu-Lin Chu
VIMS Articles
This paper describes a radiometric technique developed to measure phagocytosis of Perkinsus marinus zoospores by oyster hemocytes. The spores of P. marinus were radiolabeled by culturing P. marinus presporangia and sporangia in estuarine water (22%o) containing 14C-glycine. The percent of spores phagocytized by hemocytes was determined by the uptake of radioactivity by hemocytes.
Results from preliminary experiments to test the efficiency of using an osmotic infiltration method for immunizing oysters are also reported. It was found that oysters can take up both dissolved antigen (radiolabeled bovine serum albumin) and particulate antigen ( 14C-labeled zoospore homogenate) through osmotic infiltration. The uptake …