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Oyster Crassostrea-Virginica; In-Vitro Culture; Eastern Oyster; Extracellular Products; Protozoan Parasite
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Full-Text Articles in Life Sciences
Supplementation Of Perkinsus Marinus Cultures With Host Plasma Or Tissue Homogenate Enhances Their Infectivity, Cg Earnhart, M. A. Vogelbein, Gd Brown, Ks Reece, Sl Kaattari
Supplementation Of Perkinsus Marinus Cultures With Host Plasma Or Tissue Homogenate Enhances Their Infectivity, Cg Earnhart, M. A. Vogelbein, Gd Brown, Ks Reece, Sl Kaattari
VIMS Articles
The protozoan oyster parasite Perkinsus marinas can be cultured in vitro in a variety of media; however, this has been associated with a rapid attenuation of infectivity. Supplementation of defined media with products of P. marinus-susceptible (Crassostrea virginica) and -tolerant (Crassostrea gigas, Crassostrea ariakensis) oysters alters proliferation and protease expression profiles and induces differentiation into morphological forms typically seen in vivo. It was not known if attenuation could be reversed by host extract supplementation. To investigate correlations among these changes as well as their association with infectivity, the effects of medium supplementation with tissue homogenates from both susceptible and tolerant …