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Full-Text Articles in Life Sciences

Effects Of Sunlight On Bacteriophage Viability And Structure, Ke Wommack, Rt Hill, Ta Muller, Rr Colwell Apr 1996

Effects Of Sunlight On Bacteriophage Viability And Structure, Ke Wommack, Rt Hill, Ta Muller, Rr Colwell

VIMS Articles

Current estimates of viral abundance in natural waters rely on direct counts of virus-like particles (VLPs), using either transmission or epifluorescence microscopy. Direct counts of VLPs, while useful in studies of viral ecology, do not indicate whether the observed VLPs are capable of infection and/or replication. Rapid decay in bacteriophage viability under environmental conditions has been observed. However, it has not been firmly established whether there is a corresponding degradation of the virus particles, To address this question, viable and direct counts were carried out employing two Chesapeake Bay bacteriophages in experimental microcosms incubated for 56 h at two depths …


Growth Of Bacterioplankton And Consumption Of Dissolved Organic Carbon In The Sargasso Sea, C. A. Carlson, H. W. Ducklow Jan 1996

Growth Of Bacterioplankton And Consumption Of Dissolved Organic Carbon In The Sargasso Sea, C. A. Carlson, H. W. Ducklow

VIMS Articles

Lability of the bulk dissolved organic carbon (DOG) pool and the amount available to bacterioplankton on short time scales (hours to days) were examined in oligotrophic Sargasso Sea water (near Bermuda). We examined bacterial growth and DOC utilization using seawater culture methodology in combination with measurements of bacterial abundance, cell volume, and DOC. Bulk DOC concentrations were determined by high temperature combustion (HTC) analysis, which proved to be a sensitive method for detecting small changes in natural concentration of DOG. Measurable bacterial growth and DOC utilization only occurred in unamended cultures when initial DDC concentrations were greater than observed in …