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Genetics

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Wild And Aquaculture Populations Of The Eastern Oyster Compared Using Microsatellites, J Carlsson, Cl Morrison, Kimberly S. Reece Dec 2006

Wild And Aquaculture Populations Of The Eastern Oyster Compared Using Microsatellites, J Carlsson, Cl Morrison, Kimberly S. Reece

VIMS Articles

Five new microsatellite markers were developed for the eastern oyster (Crassostrea virginica), and allelic variability was compared between a wild Chesapeake Bay population (James River) and a hatchery strain (DEBY (TM)). All loci amplified readily and demonstrated allelic variability with the number of alleles ranging from 16 to 36 in the wild population and from 11 to 19 in the DEBY (TM) strain. Average observed and expected heterozygosities were estimated at 0.66 and 0.80 in the hatchery sample. The corresponding estimates were 0.91 and 0.75 in the wild sample. Results indicated lower genetic variability in the ...


Microsatellite Marker Development And Analysis In The Eastern Oyster (Crassostrea Virginica): Confirmation Of Null Alleles And Non-Mendelian Segregation Ratios, Kimberly S. Reece, Wl Ribeiro, Pm Gaffney, Ryan Carnegie, Standish K. Allen Jr. Jul 2004

Microsatellite Marker Development And Analysis In The Eastern Oyster (Crassostrea Virginica): Confirmation Of Null Alleles And Non-Mendelian Segregation Ratios, Kimberly S. Reece, Wl Ribeiro, Pm Gaffney, Ryan Carnegie, Standish K. Allen Jr.

VIMS Articles

Eighteen microsatellite markers were developed for the Crassostrea virginica nuclear genome, including di-, tri-, and tetranucleotide microsatellite repeat regions that included perfect, imperfect, and compound repeat sequences. A reference panel with DNA from the parents and four progeny of 10 full-sib families was used for a preliminary confirmation of polymorphism at these loci and indications of null alleles. Null alleles were discovered at three loci; in two instances, primer redesign enabled their amplification. Two to five representative alleles from each locus were sequenced to ensure that the targeted loci were amplifying. The sequence analysis revealed not only variation in the ...