Life Sciences Commons^™

A Computer Assisted Study Guide For Viral Hepatitis, April, 1999, Diana Hullihen

Theses and Dissertations

The purpose of this project was to design a computer assisted program that would enhance the effectiveness of instruction in a clinical laboratory science program. Computer assisted instruction has been proven to be a beneficial and effective method of meeting educational objectives.

The topic of viral hepatitis is a complex and difficult one to teach. The instructor decided to supplement traditional lecture using computer assisted instruction. The goals and objectives of the lesson would be reinforced through this method as well as help the student to master the subject content.

The computer assisted study guide was designed to enhance the …

Modeling Chemical Absorption Through Membranes, Jeffrey M. Hemmes

Theses and Dissertations

Understanding the processes involved in dermal penetration of chemicals and drugs is important to both toxicologists and pharmacologists. Researchers developing new drugs are interested in enhancing the penetration of chemicals through the skin, while environmental professionals are interested in limiting such penetration. For both types of applications, predictive biologically-based mathematical models can be very useful in understanding the processes involved, particularly when such models are based on physiological and biochemical parameters which can be measured experimentally. In this thesis we study two existing physiologically-based pharmacokinetic (PBPK) models that predict concentrations of neat and aqueous dibromomethane (DBM) absorbed into and through …

The Roles Of Several Kinases In Mice Tolerant To Delta-9-Tetrahydrocannabinol, Matthew C. Lee

Theses and Dissertations

It has been suggested that the CB1 G-protein-coupled receptor is internalized following agonist binding and activation of the second messenger pathways. The process of desensitization and resensitization is intimately involved with receptor internalization. Phosphorylation alters tolerance to cannabinoids thus contributing to tolerance. It is proposed that phosphorylation enhances the down-regulation of the CB1 receptor. These findings led us to look at which kinase(s) may be involved in cannabinoid tolerance. We therefore hypothesize that by preventing phosphorylation of the CB1 receptor, we may reverse tolerance. We evaluated our hypothesis by testing the role of several kinases in tolerance: protein kinase A …

Characterization Of Rat Pulmonary Carboxylesterase, Timothy J. Wallace

Theses and Dissertations

The 1839 bp cDNA for rat pulmonary carboxylesterase was cloned by reverse transcription polymerase chain reaction (RT PCR) from total rat lung RNA using specific primers derived from the 5' and 3' untranslated regions of rat hepatic cholesteryl ester hydrolase (CEH). The unique cDNA was sequenced and found to have 99% homology with hepatic CEH. This homology extends to the predicted amino acid sequences which show only six amino acid residue differences in the coding region: three conserved and three nonconserved changes. However, the catalytic activitites of the two proteins are dramatically different. While CEH hydrolyzes cholesterol oleate, the pulmonary …

Artificial Alimentation Of Bees Using Natural Juices During The Dry Season, Ramiro Bernal Acosta

Theses and Dissertations

In the community of Tunshi San Nicols of the Licto parish in the Province of Chimborazo, an evaluation was done on the effect of an energetic supplementary feeding of bees (Italian-mestizas) during the dry season (September 23 to February 20). These supplements consisted of carrot juice (T1), orange juice (T2) and sugar cane juice (T3). These supplements were also compared to a control group (T0). The experimental units consisted of 16 standard beehives distributed randomly with the 4 treatments and 4 repetitions of each treatment. The results showed that sugar cane juice (T3) obtained the best results in the consumption …

Correlation Of Visualized Glucocorticoid Receptor And Apoptosis In Individual Clones Of Nb2 Cells, Devang M. Patel

Theses and Dissertations

The Nb2 lymphoma cell line is susceptible to glucocorticoid (GC)-induced apoptosis in the absence of mitogen and this effect is inhibited by the mitogen prolactin (PRL). This process is believed to be mediated by the glucocorticoid receptor (GR) and various downstream signals. The purpose of this study was to use the limiting dilution method to isolate subsets of Nb2 clones which expressed either highly enriched amounts of GR or an absence of GR and to relate the amount of GR to the magnitude of GC induced cytolysis. The presence of GR in these clones was determined by immunocytochemistry (ICC) using …

Examination Of Signals Involved In Dexamethasone Induced Apoptosis In Nb2 Lymphoma Cells, Suhas Badarinath

Theses and Dissertations

Consistent with previous studies, we demonstrated that dexamethasone (Dex) caused cytolysis/apoptosis in log phase Nb2 lymphoma cells, while prolactin (Prl) inhibited this effect. The Nb2 model was used to investigate the mechanisms of apoptosis control with the aid of immunocytochemistry (ICC). We established with absorption the specificity of staining due to Fas, Fas Ligand, Bcl-2, and Bax antibodies (the specificity of anti-p53 could not be verified). Dex-induced cytolysis/apoptosis was detected in synchronized (G⁰/G¹) cells after 6 and 8 hours of Dex exposure. A novel, computerized technique was used to quantitate the proportion of cells immunostained for the signals of interest …

Role Of Farming Women In The Communities Of Puculpala, Llulluchi, And Guzo, Quimiag, Chimborazo, Agusta Filomena Coello Arguello

Theses and Dissertations

In the Ecuadorian society there is a pronounced difference in the distribution of work between men and women. In the Ecuadorian society the man is dominant and the woman is seen as a free form of strenuous and excessive labor, who often goes without pay. The woman herself minimizes her own contribution in the production process, thereby magnifying this culture distinction. The division of work related responsibilities, through gender distinctions, has given women more tasks to complete, in addition to those generally reserved for females (taking care of the children, the animals and various household chores). Women also tend to …

General Diagnosis And Services Developed In The Corral De Piedra Village, San Juan Ermita, Chiquimula, Servin Ardany Flores Galdámez

Theses and Dissertations

The Corral de Piedra community is located in Chiquimula, Guatemala. The main agricultural activities are corn and bean production, small-scale gardens, and small animal production, such as poultry and pigs. The crop areas are predominantly located on lands with slopes between 12 and 55%, presenting low fertility conditions and poor moisture retention capabilities. In addition, local crop varieties have low productivity potentials and the winter season is characterized by poorly distributed and erratic periods of rain. The Benson Institute introduced a technology transfer model to increase and diversify agricultural production, while preserving current natural resources. An integral part of this …

Effect Of The Partial Substitution Of A Commercial Feed For Corn Meal On Growth Performance Of Chickens, Óscar Amilcar García Portillo

Theses and Dissertations

Nutrition is a primary human need. Families often will raise their own animals to meet these nutritional needs. However, high feed costs can severely limit the viability of these small family enterprises. This study addressed ways to improve poultry production as a means for families to ameliorate their diets and increase income. Research was conducted at the Centro Universitario de Oriente (CUNORI), Chiquimula, Guatemala. For this study, commercial poultry feed was supplemented with corn flour at three treatment levels and fed to poultry. Results indicated that partial substitution of commercial feed with corn flour at these three treatment levels did …

Assessment Of The Availability And Exploitation Of Hydric Resources In The Choquenaira Community, Luis Lizardo Iturry Urquizo

Theses and Dissertations

The precipitation data of the El Alto, Tambillo and Comanche stations are homogenous and consistent. The meteorological data from Irpa Chico, Collana and Viacha are consistent, but heterogeneous. The monthly precipitation in Viacha in December, January, March and April are better examples of a normal distribution in comparison to the months of October, November and February. The average annual precipitation in Viacha, in a thirty-four year time period, is 559 mm. Some 76% of this precipitation occurs in November to March. The minimum precipitation in Viacha, with an 80% probability of absence for the vegetative period, from October to April …

Obtaining And Sampling Of High Quality Energetic-Protein Mixtures (Pottages) Of Tuber-Cereal-Leguminous Bases In The Candelaria Area, Maria Lilian Maldonado Chavez

Theses and Dissertations

Specially designed foodstuffs destined for children of weaning age in the community of Candelaria were developed and evaluated biologically. These victuals had as bases combinations of tubers-cereals-legumes of high nutritional quality to be implemented in the home. Based on the availability of food and alimentary habits of the region, seven mixtures were formulated of triples and quadruples containing tubers (potato, oca, papaliza, and isano); cereals (rice, wheat, corn, and quinua); and legumes (tarwi, broad bean, and peanut). The cereal-leguminous proportion was maintained as one complement and the addition of the tubers. These mixes were subjected to biological tests, obtaining incremental …

Detection Of Antibodies Against Brucella Abortus In Bovine, Celso Arturo Moreno Paredes

Theses and Dissertations

The present investigation was made in the Laboratories of microbiology of the Ability of Cattle Sciences of the Polytechnic Superior School of Chimborazo, with samples obtained in the Municipal Camal Riobamba and the Community of Tunshi San Nicolás. The incidence of Brucellosis was analyzed in six more important cattle areas of the county using the antigens Brucella abortus and Rosa of Flare. The experimental units were obtained under a systematic sampling. The experiment had a duration of 4 months. The experimental results underwent the following analyses: variance, percentages, stockings, standard deviation, limit for the estimate error. According to carried out …

The Relation Between The Nutritional Status And The Acute Diarrhetic Diseases In Children Younger Than Five Years Of Age In The Indigenous, Black, And Mestizo Ethnic Groups Of The Rural Area In The Imbabura Province, 1998-1999, Tanya Vaca

Theses and Dissertations

The study conducted in the rural area of the province of Imbabura included a total of 518 families that when compared with the estimated sample of 96%, 42.7 % corresponding to the indigenous ethnic group, 28.15% to Black and 25% to mixed-race ethnic groups, these differences of involvement between ethnic groups is due to the fact that the indigenous population is greater than the two remaining populations. Of the 518 families studied they were able to obtain a total of 794 children younger than 5, in which 48.5% pertaining to the male sex and 51.4% to the female sex. In …

Validation Of The Small Scale Agricultural Production Model In The Community Of Tunshi-San Nicolas, Monica Alexandra Zuniga Donoso

Theses and Dissertations

The model developed in the experimental environment inside the ESPOCH presented satisfactory results. Because of this, it is necessary that the model of production be validated in the farming communities with the purpose of making adjustments before recommending its massive production. In other words, a comparative study should be done on what happened in the experimental farm and what would happen in the community. This is reason why this investigation is titled Validation of the Small-Scale Agricultural Production Model in the Community of Tunshi-San Nicolás. The objectives of the investigation were as follows: Validate the small-scale agricultural production model of …

Religiosity And Physical Fitness: A Study Of Middle-Aged Mormon Men, Janette Olsen

Theses and Dissertations

Research has related both physical fitness and religiosity to health. This study combined these ideas and examined the relationship between religiosity and physical fitness among middle aged Mormon men (n=110). Mormons are an ideal population for study because of their strict health code. Fitness variables were grip strength, waist/hip ratio, body mass index (BMI) and estimated maximal oxygen uptake (VO2max). A questionnaire assessed level of religiosity and separated subjects into two groups: highly religious (n=35) and less religious (n=14). Moderately religious subjects (n=61) were omitted. A one way ANOVA (between-groups design) found no significant differences in fitness variables between groups. …

Determination Of The Microbiological Load Of The Principal Meat Products Sold In The Markets Of Riobamba, Miguel Angel Castillo Palacios

Theses and Dissertations

This investigation was done with the objective to determine the microbiological load of the main meat products such as: beef, chicken meat, sausage, and viena sausage which are sold in the Riobamba city markets. This investigation was carried out in the Microbiological Laboratory Livestock Science faculty of the Polytechnical Superior school. Specific chemical products were used to determine mesofilas (37 degrees C), xicrofilas (5 degrees C), and termofilas bacteria which did not develop at 45 degrees C, however exicrofilas and mesofilas bacteria were found in every market and meat product in this study. The meat product hygiene is the same …

Nutritional Education And Its Impact In The Copalacaya, Choquenaira, Callisaya And Muruamaya Communities Of La Paz, Rosa Yolanda Mamani Arias

Theses and Dissertations

This investigation was conducted with the collaboration of the authorities of the Carrera de Nutrición y Dietética (UMSA) and the Institute of Agriculture and Food Science Ezra Taft Benson. The general secretaries, mayor scholars Management 1998, rural professors and farmers of the communities of Copalacaya, Choquenaira, Callisaya and Muruamaya of the province of Ingavi of the Department of La Paz. It has been very gratifying for the author to carry out this research in said communities, in addition to knowing the reality in which these farmers live, with whose collaboration valuable information was collected that permitted an analysis on the …

Nutritional And Health Behavior Of The Indigenous Pregnant Women In The Community Of Tunshi-San Nicolas, Chimborazo Province, Sandra Ordonez Gavilanez

Theses and Dissertations

The nutritional food situation in our country is alarming, by what has been classified as a major public health problem, their principle manifestation is the protein malnutrition- energy, with a high prevalence in urban zones- marginal and rural population. It is considered that the main causes that originate on one hand are still deteriorating at a level of income in the popular sectors with the consequent impossibility of access to basic services such as housing, education, stable jobs, health services (drinking water, sewage, etc.) appropriate food regimes, greatly affecting those vulnerable groups such as children and women in fertile years. …

Therapeutical Assessment Of The Spigelia Anthelmia To Fight "Endoparasites" In Creole Ovine, German Gonzalo Reinoso Munoz

Theses and Dissertations

This investigation was accomplished in the Experimental Station Tunshi Productive Unit Sheep Goat, located to an altitude of 2747 m.s.n.m. and with an average temperature of 13.1°C, it located to 8 kilometers of Riobamba route to Licto and in the Laboratory of Microbiology of the Cattle Sciences Faculty of the Espoch, during 120 days (November of 1998 to February of 1999). The principal objective was that of to evaluate the effect terapeutics of the plant Spigelia anthelmia against parasitesinternal in lambs, for something which is 20 lambs native males with ages that fluctuated of 6 to 9 months, these animals …

Comparative Study Of Six Different Types Of Subtracts In The Production Of Earthworm Humus And Its Productive Effect In Vegetables (Radish, Beet, White Onion, Cabbage), Romeo Byron Romero Mancero

Theses and Dissertations

In the province of Chimborazo, found in the Riobamba canton, a study was done with the purpose of comparing different subtracts for the production of earthworm humus (pig feces, guinea pig feces, rabbit feces, bovine feces, market residues, and vegetable residues from the harvest) and its productive effect in vegetables (radish, onion, beet and cabbage). Twenty-four beds, 1.5 m2 (1.5 x 1) in size, as well as sixteen 3 m2 (1 x 3) parcels were used for the study. A design of complete blocks was applied with a monofactorial in the first test, and a bifactorial in the second test. …