Life Sciences Commons^™

Microcystin Accumulation In Fish Muscle Tissue: Exploring The Safety Of Fish Consumption In Several Michigan Water Bodies, Heather Snyder

Masters Theses

A variety of anthropogenic factors, including global climate change and eutrophication, are causing increases in cyanobacterial abundance. Increased prevalence of cyanobacteria can be detrimental, as some genera of cyanobacteria have the ability to produce toxic secondary metabolites known as cyanotoxins. The cyanotoxin microcystin- a hepatotoxin, is the most ubiquitous and toxic cyanotoxin in freshwater ecosystems, and has been quantified in a number of aquatic organisms. Microcystin was quantified in 8 fish species from St. Mary’s River in June, Saginaw Bay in September, and from Stony Creek Lake in October in 2014 by the Michigan Department of Environmental Quality and the …

Geochemical Control Of Methanogenesis In Cape Lookout Bight, North Carolina, Richard Kevorkian

Masters Theses

Hydrogen exerts thermodynamic control over the exclusion of methanogens by sulfate reducers in Cape Lookout Bight, NC, marine sediments. This has been demonstrated by previous in situ measurements, but has never been demonstrated in a batch incubation of unamended sediments and has never been combined with identification of the microorganisms involved in this process. We made triplicate anoxic incubations of sediments from the upper 3 cm of sediment over 122 days while taking weekly samples for DNA extraction, cell counts, and measurements of methane, sulfate, and hydrogen. The headspaces of the bottles were initially gassed with nitrogen and the third …

Comparison Of Activation Enthalpies For Aminoglycoside Modification Reactions, Brittany Sterling Soto

Masters Theses

At highly elevated temperatures, many biological reactions can proceed spontaneously from the ground state to the transition state. However, due to the long half-life of these reactions, catalysts are required to catalyze these reactions at modern day temperatures by lowering the activation energy. Wolfenden et al. has previously shown that catalysts enhance the rate of the reaction by reducing the enthalpy of activation. Therefore, the activation energies have been determined for three aminoglycoside modifying enzymes, APH(3’)-IIIa, AAC(3)-IIIb, and AAC(3)-VIa, to determine whether these three enzymes distinguish between the two classes of aminoglycoside antibiotics by reducing the enthalpy of activation during …

The Greenhouse Effect: Decomposition Rates In Human Remains Wrapped In Plastic, Barbara R. Forman

Masters Theses

Determining an accurate estimation of the postmortem interval (PMI) of human remains is important for several reasons. First, it is used to determine whether the individual is recently deceased, and therefore of medicolegal concern. If so, the estimated PMI is used in narrowing the possible identities of the deceased. PMI can also be used in excluding, or convicting, a murder suspect.

Though deviations may occur, it has been found that PMI can be calculated from the decomposition stages with reasonable accuracy (Galloway 1989, Megyesi 2001, Megyesi et al. 2005, Schiel 2008, Simmons et al. 2010). Some factors, such as low …

Enzyme Catalyzed Alginate Nanogels For Drug Delivery, Danna Nichole Sharp

Masters Theses

Developing nanoscale carriers for the delivery of therapeutics is an important topic of investigation in current biomedical research. As opposed to traditional drug delivery systems, nanoscale systems offer enhanced tissue and cell permeation in addition to reducing drug elimination from the body. Biological based therapeutics such as DNA and proteins are now widely employed in medical applications and research has focused on using nanoscale drug delivery systems to administer these more effectively. Current synthesis methods of nanoscale biotherapeutic carriers face significant challenges. Among these are creating carriers with: sizes between 10-200 nm, low polydispersity, and non-cytotoxic materials. In this thesis, …

Accuracy Of Energy Expenditure Predictions And Activity Identification In Consumer-Based Activity Monitors, James Andrew Woodman

Masters Theses

INTRODUCTION: Consumer-based physical activity (PA) monitors are increasingly common, and must be validated against criterion measures to determine which models are accurate. Such studies will aid PA intervention research and individual consumers purchasing these devices. METHODS: Thirty participants (mean ±[plus or minus] SD; age, 25.5 ± 3.7 years; BMI, 24.9 ± 2.6 kg/m2[meters squared]) completed a structured PA routine including 11 activities ranging from sedentary behaviors to vigorous intensities. During the routine participants wore an Oxycon portable calorimeter (criterion measure of energy expenditure (EE)), a Basis Peak and Garmin Vivofit on the non-dominant wrist, and three Withings Pulse devices (right …

Economic And Environmental Optimization In The Supply Of Switchgrass In Tennessee, Jia Zhong

Masters Theses

The low efficiency of collection, storage and transportation in the switchgrass supply chain has hindered the commercialization of a switchgrass-based biofuel industry, even given its ecological and environmental advantages in carbon sequestrate, soil quality, water use, and pollution pressure. Thus, designing a switchgrass-based supply chain balancing both environmental and economic performance is important to expedite the development of the cellulosic biofuel industry to meet the national energy plan.

The objectives of this study are to 1) determine economic cost and multiple environmental outcomes in feedstock supply chains and 2) identify the relation between the economic and environmental performances. The first …

Gallic Acid-Grafted Chitosan Films As Antioxidant Food Packaging, Ping Guo

Masters Theses

Chitosan is the second most abundant natural polysaccharide in the nature. Due to its biodegradability and film forming ability, chitosan has the potential to be used as an alternative to petroleum-based polymers for food packaging. The presence of a primary amine as well as primary and secondary hydroxyl groups enable chitosan to be chemically modified with various functional groups. Gallic acid (GA) is a natural occurring antioxidant (AOX), which can be grafted to chitosan using by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The first project deals with the effect of ethanol (EtOH) concentration on efficiency of grafting GA onto chitosan. …

Evaluating Accuracy And Bias In Genetic Monitoring Under Scenarios Of Population Decline, Susan K. Munster

Masters Theses

Accurate and unbiased estimates of current effective population size are of primary importance in making informed decisions for conservation purposes. One species of concern, the eastern red bat (Lasiurus borealis), is experiencing ongoing population losses due to wind turbine collisions. The proportion of the population being affected is currently unknown, although recent estimates of total fatalities at wind turbines over the time from 2000-2011 are in the hundreds of thousands for this species alone. The roosting habits of eastern red bats make it difficult to monitor their census population size (Nc); thus, genetic estimates of effective population size (Ne) may …

The In Vivo Effect Of Osmolytes On Folate Metabolism, Timkhite-Kulu Berhane

Masters Theses

Previous studies have found that addition of osmolytes weakens the binding of dihydrofolate (DHF) to R67 dihydrofolate reductase (DHFR), chromosomal DHFR from E. coli and a pteridine reductase. These results support the preferential interaction of DHF with osmolytes compared to water. Thus, a working model where interaction of DHF with osmolytes shifts the binding away from the protein-DHF complex towards the free species was proposed. As tetrahydrofolate and other folate redox states have similar structures to DHF, we predict osmotic stress will lower the catalytic efficiencies of other folate pathway enzymes. In this thesis, we explore the in vivo effects …

Mechanisms For Social Influence, Jeremy David Auerbach

Masters Theses

Throughout the thesis, I study mathematical models that can help explain the dependency of social phenomena in animals and humans on individual traits. The first chapter investigates consensus building in human groups through communication of individual preferences for a course of action. Individuals share and modify these preferences through speaker listener interactions. Personality traits, reputations, and social networks structures effect these modifications and eventually the group will reach a consensus. If there is variation in personality traits, the time to reach consensus is delayed. Reputation models are introduced and explored, finding that those who can best estimate the average initial …

Exploring How Clean Water Act Enforcement Influences Stream Macroinvertebrate Communities, Shelby Renee Burks Ward

Masters Theses

Enforcement is a key feature of the Clean Water Act. The Clean Water Act’s National Pollutant Discharge Elimination System (NPDES) permit program regulates effluent into streams that may impact aquatic life. Yet, authorities do not always enforce permits when violations occur. My research examines macroinvetebrate survey data near NPDES permitted facilities in Kentucky and Tennessee to determine the influence of enforcement actions on aquatic biodiversity. Pre-existing data from the Environmental Protection Agency and state agencies was used to make quantitative and spatial comparisons.

My first study used multiple regression to explore whether enforcement and freshwater community indicators changed together. North …

Comparison Of Seed Yield, Oil And Phenotypic Traits Among Selected Parents And Crosses Of Niger, Victoria Grace Benelli

Masters Theses

Niger (Guizotia abyssinica (L.f) Cass.) is primarily marketed as favorite seed among American goldfinches (Carduelis tristis) as well as pine siskin, redpoll, house finches, and ground feeding birds like quail and dove. As a part of a balanced diet, it is crucial for these species to consume a higher percentage of fats than other bird species. Fourteen niger accessions of Indian, Ethiopian, and American origin were obtained from USDA/ ARS germplasm collection at Pullman, WA and planted in August 2012 at the East Tennessee Research & Education Center. Five of these accessions were crossed to form different …

Development Of Cross Laminated Timber In The United States Of America, Karl Konstantin Grasser

Masters Theses

This research focuses on the establishment of Cross Laminated Timber (CLT) ventures in the United States of America (USA) and provides interested stakeholders knowledge about the product and the existing CLT industry. This research is designed to improve knowledge of CLT and manufacturing technologies for potential investors of CLT capacities in the USA.

The invention of CLT in Austria led to a paradigm change in European wood construction as it allowed the woodworking industry to enter new construction markets such as multi-story residential and non-residential buildings. The CLT industry has experienced tremendous growth in Europe. The United States is a …

Dietary Supplementation Of Choline And Betaine In Heat-Stressed Broilers, Kouassi Remi Kpodo

Masters Theses

A study was conducted to determine the effects of supplemental choline (CHO) and betaine (BET) on broiler performance, carcass characteristics, corticosterone levels, immune organ weights, intestinal morphology and choline metabolites under thermoneutral (TN: 23.9^oC) and heat stress (HS: 28-36^oC) conditions. Eight hundred day-old chicks (400 per temperature treatment) were assigned in groups of 10 to each of five dietary treatments: 1 (CON) the basal diet; 2 (CHO500), basal diet plus 500 ppm methyl equivalents added CHO; 3 (CHO1000), basal diet plus 1000 ppm methyl equivalents added CHO; 4 (BET500), basal diet plus 500 ppm methyl equivalents …

Development And Evaluation Of Novel Vaccination Strategies For Campylobacter Control In Poultry, Xiang Liu

Masters Theses

Chicken is the primary natural host of Campylobacter, the leading bacterial cause of human enteritis in the US and other developed countries. Thus, mitigation of Campylobacter in chicken using innovative approaches, such as vaccination, will have a significant impact on food safety and public health. Our previous studies have demonstrated that the two outer membrane proteins, CmeC (the essential component of the CmeABC multi-drug efflux pump) and CfrA (a ferric enterobactin receptor), are feasible candidates for immune intervention against Campylobacter. DNA vaccine has appeared to offer various advantages for poultry, particularly when combined with in ovo vaccination. Chitosan-encapsulated …

Population Structure Of Late Blight (Phytophthora Infestans) In Colombia And Ecuador And Downy Mildew (Peronospora Farinosa F. Sp. Spinaciae) On Spinach In Arizona And California, Rebecca Marie Lyon

Masters Theses

In this study, Phytophthora infestans and Peronospora farinosa f. sp. spinaciae populations were analyzed using single nucleotide polymorphisms (SNPs). In Ecuador and Colombia, Phytophthora infestans causes significant damage to potato and tomato and the epidemiology is known to be highly clonal. Our objective was to measure population structure within the context of this clonal epidemiology using both synonymous and nonsynonymous markers. Candidate SNP sites were selected by comparing the draft genomes of the Ecuadorian isolates EC1-3527 and EC1-3626. Genotypes were assessed directly from infected tissue using a targeted sequencing approach. A total of 54 polymorphic sites were assessed in 93 …

The Effect Of Perceived Healthy And Unhealthy Commercials On Intake Of Perceived Healthy And Unhealthy Snack Foods In Normal Weight, College-Aged, Dietary Restrained Women, Seletha Ann Periman

Masters Theses

Background: Exposure to various types of food cues via television commercials may enhance consumption of different foods, particularly in females who engage in cognitive efforts to control food intake (i.e., restrained eaters). However, it is unknown if food-related commercials differing in health perceptions impact on intake of foods with differing health perceptions. This study investigated the effect of viewing “healthy” or “unhealthy” food commercials on intake of “healthy” and “unhealthy” snack foods in healthy weight, dietary-restrained, college-aged women.

Methods: Using a 3 x 2 between-subjects design (factors of commercial type [“healthy” foods, “unhealthy” foods, and non-food-related] and snack food type …

Evaluation Of The Thermal Inactivation Kinetics Of Surrogate Vegetative Bacteria, Amber Lynne Parham Renix

Masters Theses

Microbial surrogates are defined as non-pathogenic organisms with behavioral and/or survival characteristics correlating to those of specific pathogens. The purpose of this study was to determine the thermal inactivation kinetics (D and z-values) of Lactobacillus bulgaricus ATCC 11842, Lactobacillus fructivorans ATCC 8288, Lactobacillus plantarum ATCC 4008, Listeria innocua ATCC 33091, ATCC 51742, and 11/97, and Staphylococcus carnosus CS-299 as potential surrogates for hepatitis A virus (HAV; D_72°C = 0.9 min). Additionally, the effect of sub-lethal heat on increasing the thermotolerance of L. bulgaricus was investigated. All strains were tested in phosphate buffered saline (PBS) as a …

Survival And Growth Rate Of Translocated Freshwater Mussels Lampsilis Fasciola And Medionidus Conradicus, Laura L. Pullum

Masters Theses

Freshwater mussels (Family Unionidae and Margaritiferidae) are a widely threatened group of bivalve molluscs, particularly in the Southeastern United States. Translocation of freshwater mussels is an increasingly common conservation method. However, there are relatively few studies that quantitatively investigate the factors influencing translocation success or failure. In October 2013, hundreds of Medionidus conradicus and Lampsilis fasciola were translocated to the Pigeon and Nolichucky Rivers in Tennessee, with an interim partial survey (June 2014) and a full survey (October 2014). In this study, I analyze this field-collected data to determine the mechanism(s) that currently influence the outcomes of Tennessee mussel translocation. …

Do Increases In Fruit Intake Lead To Substitutive Eating Patterns And Decreases In Overall Energy Intake In Normal Weight Adults?, Shireen Amanda Sobhani

Masters Theses

Background: It is suggested that increasing intake of fruits and vegetables may lead to decreased energy intake via substitution of higher energy-dense foods, such as snack foods (SF; i.e. candy, chips, cookies). This study investigated the impact of increasing fruit (F; grapes) intake, via increased portion size, on SF (potato chips) intake during a meal and whether increases in F intake affected total meal energy intake, via reducing SF intake (substitutive eating), in normal-weight adults. A secondary objective investigated whether the relative reinforcing value (RRV) of SF was a moderator of the substitutive relationship between F and SF.

Methods: Using …

Manipulating Adipose Tissue Fatty Acid Oxidation To Reduce Fatness In Broiler Chickens, Tania Emmanuelle Torchon

Masters Theses

Compared to rodents, broiler chickens, those reared for meat, are an attractive model for studies of adipose biology, and obesity development in children. The broiler chicken lacks the gene for uncoupling protein 1, the hallmark for brown adipose tissue making them a useful model to study lipid metabolism in white adipocytes. Two studies were performed to investigate if white adipose tissue had the metabolic ability for fatty acid oxidation (FAO), and to investigate the effects of dietary fatty acids on abdominal fat development of young broiler chickens as a model for childhood obesity. In study one, chickens were fasted for …

Exploring Connections Between Prenatal Physical Activity And Breastfeeding, Emily Alexandria Tucker

Masters Theses

Women who decide to engage in prenatal physical activity are giving themselves, and their child, an advantage over those who are not physically active during pregnancy; not only are there significant health benefits for mothers, but also there are great benefits for the unborn child. Breastfeeding is another important decision parents make that can impact the future health of their children. There has been extensive research on the benefits of both prenatal physical activity and breastfeeding, but the connection between the two is unclear. The purpose of this grounded theory study was to identify potential connections between factors influencing women’s …

A Comprehensive Evaluation Of Benthic Invertebrate Communities In The Emory River, Watts Bar Reservoir, Tn, Suzanne Jane Young

Masters Theses

The release of fly ash at the Tennessee Valley Authority (TVA) Kingston Fossil Plant (KIF) on 22 December 2008 discharged approximately 4.1 million cubic meters of coal ash into the adjacent aquatic and terrestrial systems. Previous benthic invertebrate investigations conducted by TVA and collaborative researchers concluded that benthic invertebrates in the Emory River were at moderate risk from ash-related constituents, primarily arsenic, in ash-contaminated sediment that remained in the Emory River following extensive dredging efforts. These conclusions were based on the observation of statistically significant reductions in growth and biomass in laboratory toxicity tests with Emory River sediment. Benthic invertebrate …

Conformational Dynamics Of Cytochrome P450cam Upon Ligand Binding, Ana Virginia Bernal Gomez

Masters Theses

Cytochrome P450s are a superfamily of metalloenzymes that are responsible for the monooxygenation of their hydrophobic substrates. P450’s retain the same general structural scaffold, however are able to bind promiscuously to substrates with distinct physico-chemical properties. It is believed that they possess considerable flexibility in the substrate binding regions as well as their active site to accomplish the diverse binding and catalytic chemistry with high regio- and stereo-specificity. In order to investigate the conformational dynamics inherent in these enzymes, especially in context of binding different ligands, we carried out amide proton exchange studies via NMR spectroscopy on a model P450 …

Bat, Insect Prey, And Vegetation Response To Prescribed Fire And Overstory Thinning In Hardwood Forests Of Tennessee, Maxwell Rambeau Cox

Masters Theses

This master’s thesis investigates the effects of prescribed fire and overstory thinning on bats and their insect prey in hardwood forest stands of Tennessee. Chapter 1 is a review of literature that emphasizes the importance of this research and outlines the objectives and study area for this project. Chapter 2 examines the effect of prescribed fire and overstory thinning on the abundance and biomass of nocturnal flying insects important in the diet of bats. Overall, I found prescribed fire and overstory thinning had little effect on nocturnal flying insect abundance and biomass, despite changes in vegetation community composition and structure. …

Nutrition Marketing On The Front-Of-Package: 2007-2014, Ana Elizabeth Hoffmann

Masters Theses

Objective: The objectives of this research were to describe the proportion of packaged foods with front-­‐of package nutrition marketing or marketing to children that were high in saturated fat, sodium, and/or sugar content, and to delineate changes in the proportions of these foods from 2007 to 2014.

Methods: Front-­‐of-­‐pack nutrition marketing, child marketing, and nutrient criteria from the Nutrition Facts Label on all packaged food items in a large Midwestern supermarket were recorded in 2007 (n = 5,500) and again in 2014 (n = 6,324). Products were coded as high/not high in saturated fat, sodium, and/or sugar according to agreed …

My Painted Plate: Nutrition-Related Art Enhances The Effects Of Nutrition Education, Abby Helene Forman

Masters Theses

Background: A few studies have shown that art–based nutrition education (primarily in the form of theater) can increase nutrition knowledge and change behavior. Little research exists on the use of visual arts in nutrition education programs. The purpose of this study was to determine the impact on children’s nutrition knowledge, self-efficacy, and behavior, of using visual art and differing levels of intensity of art activities in nutrition education.

Methods: The study used a three-armed cluster randomized control trial design with three time points. Children ages 8-12, at 6 different summer camps in Knoxville, Tennessee, attended the My Painted Plate program. …

Invasive Asian Earthworms Negatively Impact Woodland Salamanders: Competitive Exclusion, Foraging Interference, And Habitat Degradation Reduce Salamander Density, Julie L. Ziemba

Masters Theses

Asian earthworms (Amynthas spp.) are invading North American forests and consuming the vital detrital layer that forest floor biota (including the Eastern Red-backed Salamander, Plethodon cinereus) rely on for protection, food, and habitat. Salamander population decline has been associated with European earthworm-mediated leaf litter loss, but there have been few studies on the interactions between Amynthas spp. and P. cinereus. Since the large, active Amynthas spp. earthworms spatially overlap with salamanders beneath natural cover objects and in detritus, they may compound the negative consequences of resource degradation by physically disturbing important salamander activities (foraging, mating, and egg brooding). I predicted …

Great Lake Island Biogeography Of Plethodon Cinereus: Effect Of Island Elevation On Color Polymorphism, Cameron N. Eddy

Masters Theses

Islands have long fascinated ecologists and evolutionary biologists because they provide replicated systems with which to test hypotheses regarding evolutionary and ecological theory. Because islands are relatively simple systems, they enable researchers to isolate specific factors responsible for observed phenomena. Many current questions in ecology and evolutionary biology have been addressed in island systems. For example, factors involved in the genetic divergence of populations are easier to discern in island populations because confounding effects of gene flow from adjacent populations are limited. This study focuses on isolated island populations of the color polymorphic Eastern Red-backed Salamander (Plethodon cinereus) …