Life Sciences Commons^™

Serotonin And Its Effects On The Chronic Stress Response In Crayfish Following Nonylphenol Exposure, Tara M. Olen

Masters Theses

Current research suggests that stress in both vertebrates and invertebrates is modulated in part by the neurotransmitter serotonin. It has been shown that crayfish can function as a good neurophysiological model, as they have less complex neurological systems than vertebrates, so mechanistic causes for stress can be more readily studied and understood. One potential stressor for aquatic species such as crayfish would be nonylphenol, a hydrophobic chemical used in agricultural products that can make its way into the water supply due to agricultural runoff. Nonylphenol can lead to physiological and behavioral impairments in crayfish and these impairments likely induce stress …

Morphological Characterization Of Two Transgenic Strategies For Genetic Access To Semilunar Granule Neurons In The Mouse Dentate Gyrus, David T. Rexford

Masters Theses

Granule cells (GCs) of the dentate gyrus (DG) have been understood as a homogeneous class of neurons exhibiting a characteristic limited firing pattern. A subtype of GC called a semilunar granule cell (SGC) has been identified exhibiting variant morphology, electrophysiology, and positioning from normal GCs. SGCs represent an emerging novel subpopulation of GCs, however, there is presently no genetic tool to access SGCs separately from normal GCs. To provide access for future in vivo studies of this population, we examined two genetic strategies for putative SGC specificity in mouse brain slices. Morphological analysis was performed for quantitative identification of putative …

The Impacts Of Road Salt On Water Quality And Phosphorus Dynamics In An Urban Lake, Ellen Foley

Masters Theses

Road salt runoff from de-icing applications has notably increased chloride concentrations in lakes throughout north temperate regions of the planet, with negative impacts on freshwater ecosystems. For the past 20 months, I have monitored the water quality of a chloride-impaired lake and associated tributary in Grand Rapids, Michigan. Chloride levels in the deepest part of the lake have reached up to 331 mg/L, above EPA chronic toxicity thresholds. The salt-induced density gradient has prevented the lake from completely mixing during my study period and created persistent hypoxia in the hypolimnion. Total phosphorus (TP) concentrations in the hypolimnion can exceed 7500 …

The Application Of Microbial Source Tracking To Aid In Site Prioritization For Remediation In Lower Michigan, John J. Hart

Masters Theses

Non-point source fecal pollution is a threat to both the environment and public health. Climate change, aging infrastructure, and intensified agricultural practices are predicted to accentuate this issue. In Michigan, due to the high instance of aging infrastructure and intensified agriculture, non-point source fecal pollution has caused many waterbodies to exceed the state standards posing a risk to recreational activities and source water. Due to this threat, there is an increased effort to identify and remediate these sources. My study focused on improving the identification of non-point source fecal pollution through a combination of culture-based and molecular fecal indicator bacteria …

Benthic Macroinvertebrate Pre-Assessment Of A Proposed Restoration In The Grand River, Grand Rapids, Mi, Usa, Matthew L. Bain

Masters Theses

River restoration is the dominant field of applied water resources management in the United States. Ecological improvement should be the goal of all river restorations, though many restoration projects fail to produce positive results due to limited scope or inadequate assessment methodologies. Pre-restoration, biotic data is essential for such projects as it can be paired with post-restoration data to gauge ecological outcomes. A major restoration effort is now underway in Michigan's longest river, the Grand River, where it flows through downtown Grand Rapids. The primary restoration measures will occur in-stream at local scale. We conducted a pre- restoration survey for …

The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey

Masters Theses

The ability of plants to absorb light through photoreceptors is essential for successful plant development. Phytochromes are important plant photoreceptors that detect and absorb red and far-red light and must function properly for plant survival. The Light Response BTB proteins (LRB) play an important role in a plant’s response to red light by degrading phytochrome B (PhyB) via the ubiquitin proteasome system (UPS). Although there has been a successful investigation into understanding the function of the LRBs, the function of a few regions within LRB proteins remains unknown, including a portion located on the N-terminal end of the protein, termed …

Using Plot Photographs To Estimate Tundra Vegetation Cover In Northern Alaska, Hana Christoffersen

Masters Theses

Plot photography can provide a quick, robust method to measure vegetation, especially in polar environments where logistics can be expensive and challenging. The success and widespread adoption of plot photography in the Arctic hinges on the accuracy of image analysis and data product interpretation. The relative cover of eight vegetation classes was estimated using a point frame and digital camera across thirty, 1-m² plots at Utqiaġvik, Alaska from 2012 to 2021. Geographic object-based image analysis (GEOBIA) was applied to generate objects and classify the three band (red, green, blue) images. Machine learning classifiers (random forest, gradient boosted model, classification …

The Spatial Ecology And Habitat Selection Of A Spotted Turtle (Clemmys Guttata) Population In Southwest Michigan, Michela Coury

Masters Theses

Turtles are arguably the most threatened group of vertebrates on the planet. Anthropogenic influences such as habitat loss and fragmentation, road mortality, subsidized predators, climate change, and illegal collection have contributed to their global decline. Understanding which isolated populations of turtles are viable despite these synergistic threats is crucial for making conservation and management decisions. In Michigan, the spotted turtle (Clemmys guttata) is a threatened species subjected to many negative anthropogenic influences that accelerate its rapid population decline. In order to properly sustain their populations, the goal of my research was to investigate relevant factors that help us …

Microbial Experience Increases Cytotoxicity Of Tumor-Infiltrating Cd8+ T Cells And Controls Tumor Growth, Nicholas Aaron Bunda

Masters Theses

Cancer immunotherapy research is traditionally conducted with specific pathogen-free (SPF) mice, which most accurately mimic the immune system of a human newborn. This makes translational research challenging, as this mouse model is not an accurate reflection of the adult patients who ultimately receive newly developed treatments. It is necessary to further develop a mouse model that bridges this gap and increases translatability of current cancer immunotherapy research.

By cohousing specific pathogen-free mice with regular pet store (PS) mice, we have generated a cohoused (CoH) mouse that reflects the microbial experience of an adult human immune system. We investigated antigen experience, …

Comparing Macroinvertebrate Total Abundance And Total Biomass On Five Substrate Types From Upstream To Downstream On The North Branch Of The Au Sable River, Paul David Dingman

Masters Theses

The North Branch of the Au Sable River is located in the northern lower peninsula of Michigan and is known for prolific hatches of Ephemeroptera (mayflies), Trichoptera (caddisflies), and Plecoptera (stoneflies). Macroinvertebrates play an important role in processing and recycling organic material in rivers and are a valuable food source for trout. In 2018, anglers were reporting catching fewer numbers of brook and brown trout (Salmo trutta and Salvelinus fontinalis). The Michigan Department of Natural Resources (MDNR) determined significantly lower abundance than the historical average. We hypothesized that trout abundances were lower due to a lack of prey …

Does Epa Cause A Decrease In Inflammation Of Bend.3 Cells Through Ffar4?, Clay J. Weidenhamer

Masters Theses

Atherosclerosis is an inflammatory disease initiated by low and oscillatory shear stress on the endothelium. The inflammatory process recruits leukocytes to the vessel wall by expression of the adhesion molecule VCAM-1. Activation of the NF-κB inflammatory signaling pathway is responsible for the increase in VCM-1 expression. Omega 3 FAs, such as EPA, reduce the risk of atherosclerosis by decreasing this inflammatory response. The pathway by which omega 3 FAs is proposed to inhibit inflammation includes activating FFAR4 to decrease NF-κB activation thereby reducing expression of adhesion molecules. We hypothesized that treatment of endothelial cells with 30 μM EPA would decrease …

Assessing The Short-Term Effects Of Translocation On Freshwater Mussels: Is Habitat Or Water Quality More Important?, Joshua D. Arnold

Masters Theses

Freshwater mussels (Order: Unionida) are very important to the function of aquatic ecosystems and are typically indicators of good water quality. They provide a valuable link between the water column and the benthic substrate in which they live and are a valuable food resource for many species of animals. However, most species native to North America are currently threatened with extinction, to the point that more than 70% of native freshwater mussels are listed as either threatened or endangered at the state or federal level. The cause of this decline can be attributed to historical over exploitation, habitat alteration, and …

Pedigree And Molecular Assessment Of Relatedness In A Captive Chimpanzee Population, Francesca Golus

Masters Theses

As a crucial component of biodiversity, genetic diversity contributes to variability among individuals, allowing populations of endangered species to be resilient in the face of changing environmental conditions. Zoological institutions have become a cornerstone of conservation efforts and a refuge for endangered species given threats imposed on wild populations by climate change, habitat fragmentation and degradation, and overexploitation. Chimpanzees (Pan troglodytes) are an endangered species experiencing drastic population decline in the wild, yet are common residents in zoos and wildlife sanctuaries. To sustain long-term, genetically viable populations of species of concern, zoos use studbooks and paternity testing to …

Effects Of Am Fungi From Conventional And No-Till Michigan Crop Fields On Plant And Soil Health, Derek Bennett

Masters Theses

Centuries of conventional till (CT) management in agriculture has depleted soil organic matter (SOM) by over 50%. While only comprising 5% in most soils, SOM provides soil with fertility and productivity. To compensate for SOM depletion, producers have been forced to increase their reliance on fertilizer and irrigation to maintain yields. In the coming decades, climate change is expected to challenge food production and threaten an already fragile system. With no remaining land left to cultivate, conservation management strategies such as no-till (NT) look to restore SOM and increase the resilience of food production for an ever growing, increasingly food …

Evaluation Of Lake Sturgeon (Acipenser Fulvescens) Spawning Success On An Artificial Reef Constructed In The Kalamazoo River, Michigan, Jason P. Lorenz

Masters Theses

Since lake sturgeon Acipenser fulvescens monitoring began on the Kalamazoo River in 2009, there has been no documentation of survival past the egg stage. Spawning has occurred directly below Calkins Dam and, in 2016, an artificial spawning reef was constructed 275-m downstream to improve habitat and increase survival to the larval stage. The site was monitored with egg mats and larval drift nets to document evidence of successful natural reproduction of lake sturgeon one year prior to construction (2016) and three years post-construction of the artificial reef (2017-2019). Fertilized lake sturgeon eggs were captured all four years of the study …

Fecal Findings: Investigating Eastern Massasauga Rattlesnake Diet Using Dna Metabarcoding, Alyssa Swinehart

Masters Theses

Characterizing the diet of imperiled species using minimally invasive methods is crucial to understanding their conservation requirements. DNA metabarcoding methods have been used to characterize the diet primarily in mammalian systems, while reptiles are heavily underrepresented in this literature. Here, we apply a DNA metabarcoding approach to study the diet of the eastern massasauga rattlesnake (Sistrurus catenatus); a Federally Threatened snake found throughout the Great Lakes Region. Eighty-three fecal samples collected across 10 different massasauga populations located in Michigan were sequenced. We use universal metazoan primers and develop a host-specific oligonucleotide blocker to uncover the full potential diet …

Use Of Imaging Flow Cytometry To Assess The Hematology Of Eastern Massasauga Rattlesnakes (Sistrurus Catenatus), Jennifer H. Kovach

Masters Theses

The federally threatened eastern massasauga rattlesnake is faced with many threats, including the newly recognized snake fungal disease. Unfortunately, the immune response to the disease is unknown. Leukocyte profiles are used as health assessment tools for many species, and imaging flow cytometry can be used as an application to characterize leukocyte profiles. Our goal was to use imaging flow cytometry to identify leukocyte profiles of eastern massasauga rattlesnakes and determine differences in profiles between sexes, reproductive status, ages, location, and individuals with clinical signs of snake fungal disease. Blood samples from 201 individuals were collected from ten locations throughout the …

Spatial And Demographic Patterns Of Two Threatened Turtle Species In An Urban Environment, Carly E. Brouwers

Masters Theses

Urban development is a global threat to native wildlife. The process of urbanization reduces and degrades the useable habitat of a region, and creates novel “urban ecosystems” that possess new threats and stressors to local species. Turtles are one of the most threatened vertebrate groups worldwide, and are particularly at risk of decline in urban ecosystems due to reduced nesting success, increased road mortality events, altered movement patterns, and increased predation rates. Eastern box and Blanding’s turtles are two at-risk turtle species in the state of Michigan, USA, primarily due to land use change. Presently, there are urban populations of …

Analysis Of Seasonal Changes In Thermal Stress Resilience And Innate Immunity In The Temperate Coral, Astrangia Poculata, From Future Climate Impacts, Tyler Eugene Harman

Masters Theses

Over the years, global warming has had a devastating effect on coral reef ecosystems. Anthropogenic influences have caused significant increases in greenhouse gases, with a subsequent increase in solar radiation held within Earth’s atmosphere leading to increasing global temperatures. The increasing temperatures from concurrent increases in greenhouse gases impact fragile marine ecosystems such as coral reefs, which require particular environmental parameters such as temperature in order to survive and maintain a diverse ecosystem in which many marine species rely on. These increases in temperature exacerbate phenomena such as bleaching events and coral disease, drastically impacting coral on a global scale …

Hormonal Responses To Seasonal Thermal And Ecological Stressors In Japanese Macaques (Macaca Fuscata), Lilianne Nelson

Masters Theses

Relative to most primate species, Japanese macaques (Macaca fuscata) experience extreme seasonal variation in thermal and ecological stressors that can impact energetic demands. To cope with these environmental changes, levels of metabolic hormones, such as cortisol and triiodothyronine, fluctuate to facilitate energetic adjustments. While previous research in primates has investigated thermal and ecological stressors individually, a combined assessment of these stressors alongside hormone levels can provide a more holistic understanding of the relationship between a primate’s thermoregulation, energetic balance, and stress. The goals of this study were to determine the effects of season, temperature and ecological stress on …

Drivers Of Decadal Vegetation Change In Northern Alaska, Jacob Allen Harris

Masters Theses

The Arctic is experiencing rapid climate change. This research documents vegetation change near Atqasuk and Utqiaġvik, Alaska. At each location, 30 plots distributed in a matrix across the landscape, were sampled annually from 2010 to 2019 using a point frame. For every encounter we recorded the height and classified it into eight broad functional groups (deciduous shrubs, evergreen shrubs, forbs, graminoids, bryophytes, lichens, litter and standing dead vegetation); for vascular plants we also identified the species. We found a consistent increase in plant stature and cover over time which was dramatic at Atqasuk. Graminoid cover and height increased at both …

The Impact Of Microbial Experience On The Murine Innate Immune Response, Cody Thomas Morrison

Masters Theses

The hygiene hypothesis predicts that certain environmental factors shape overall immune system function in animals and humans. While current specific pathogen free (SPF) mouse models are invaluable for studying the immune system, they have limitations for comparison with humans who have microbial exposures throughout their lifetimes. Several studies have shown that the composition of the immune system of SPF mice more closely resembles that of newborns, whereas the immune system from mice exposed to microbial pathogens more closely reflect adult immunity. In this study we have established a model using traditional SPF mice (“clean mice”) and SPF mice that were …

Great Lakes Snake: Estimating The Occupancy And Detection Probabilities Of The Eastern Massasauga Rattlesnake (Sistrurus Catenatus), Arin June Thacker

Masters Theses

Detailed knowledge of a species’ range and distribution is important for understanding species persistence and developing species management plans. This issue is particularly pronounced in threatened species with wide-spread range and a low detectability in their natural environment, as surveying and successfully encountering this type of species is oftentimes difficult. One such species is the eastern massasauga rattlesnake (Sistrurus catenatus), a smallbodied pit viper with a distribution centered around the Great Lakes region. We used singleseason occupancy modeling in order to reassess the status of historic massasauga occurrences. We evaluated factors affecting eastern massasauga detection probability from a …

The Investigation Of Group 1 Late Embryogenesis Abundant Protein 6 And Its Role In Arabidopsis Thaliana Desiccation Tolerance, Joann Eise Thomson

Masters Theses

Like most seeds, Arabidopsis thaliana seed development is divided into three major phases: morphogenesis, storage deposition, and maturation drying. During maturation drying the seed becomes desiccation tolerant (DT) at some point and the seed rapidly loses water until its moisture level is approximately 10% on a fresh weight basis. The ability of the seeds to withstand this extreme water loss is hypothesized to be due, in part, to the accumulation of late embryogenesis abundant (LEA) proteins. In A. thaliana, there are 51 LEA proteins which are divided into seven groups based on amino acid sequence similarities. Group 1 LEA …

From The Dinner Pot To Smoking Pot; How A Better Understanding Of Cannabidiol In Crayfish Could Alleviate Anxiety And Modulate Hunger, Christopher Michael Timmer

Masters Theses

Anxiety affects approximately 1/3 of the US population and presents in many different forms, ranging from social to panic disorders. It also presents with high comorbidity for other mental disorders. One treatment is Selective Serotonin Reuptake Inhibitors (SSRIs) which allow for increased activation of serotonin (5-HT) receptors. SSRIs come with an extensive list of side effects, which can fail to maintain quality of life. Cannabidiol (CBD) is a cannabis derived compound which has been shown to decrease anxiety by activation of multiple subtype 5-HT amine receptors. CBD has few side effects, is not psychoactive, and exhibits anti-psychotic properties. The current …

Carbon Sequestration In A Restored West Michigan Oak Savanna: Implications For Management Practices, Jeffrey A. Heise

Masters Theses

The savanna system is an ecosystem (i.e. a transitional ecosystem) that lies between forest and grassland ecosystems. They occur across the world in various forms, but in the North American Midwest they are specifically oak savannas: systems where the open overstory is dominated by various species of oak (Quercus spp.) and the understory consists of carbon-rich prairie grasses and forbs. This ecosystem is a highly degraded ecosystem and has lost almost 99% of its former range due to agriculture and fire suppression. Since savannas are fire-evolved systems, they are maintained by and require fire as a regular disturbance to …

Competitive Interactions: Spatial And Temporal Niche Partitioning Between Brook And Brown Trout In Southwest Michigan, Frederick J. Glassen

Masters Theses

Niche diet partitioning between Brook Trout and Brown Trout were studied in two southwestern Michigan, USA streams, one dominated by Brook Trout, the other with both Brook and Brown Trout. Brook Trout are endemic to eastern North America and have been introduced throughout the world for sport fishing. Brown Trout were first introduced to Michigan in 1883 using a genetic strain from Germany. Stomach contents were collected from a population of Brook Trout in the absence of Brown Trout in Frost Creek, while the other population was in potential competition with Brown Trout in Cedar Creek. Absent Brown Trout, Brook …

Bloom Or Bust: Search For Phytoplankton Community Drivers Using Long-Term Time-Series Observations And Field Measurements In A Model Great Lakes Estuary, Jasmine Mancuso

Masters Theses

As sentinels of climate change and other anthropogenic effects, freshwater lakes are experiencing ecosystem disruptions at every level of the food web, beginning with the phytoplankton. One of the major threats to waterbodies around the world are cyanobacterial harmful algal blooms (HABs) resulting from anthropogenic eutrophication and exacerbated by climate change. Muskegon Lake, a drowned river mouth Great Lakes estuary on the east coast of Lake Michigan, is no exception and was declared an Area of Concern by the EPA in 1987 with nuisance algal blooms cited as a beneficial use impairment. Using long-term data and additional 2019 sampling, we …

Variation In Tundra Plant Traits Across A Latitudinal Gradient, Katlyn Rose Betway

Masters Theses

High latitude regions are warming faster than most regions. Studies documenting change in plant cover due to warming have reported that graminoids, deciduous shrubs, and evergreen shrubs are increasing in some regions of the Arctic, but not at others. Mixed responses to warming have caused researchers to shift towards an emphasis on functional traits of individual species rather than their growth forms. This thesis focuses on ten measured plant functional traits for twelve arctic species at three regions spanning a latitudinal gradient in northern Alaska (Utqiaġvik, Atqasuk, and Toolik Lake). We compare mean trait values across the three regions for …

Detection Probability Of The American Marten (Martes Americana) In Michigan’S Lower Peninsula, Taylor Brian Root

Masters Theses

To accurately manage for the sustainability of wildlife populations, managers must first determine how to locate individuals of a population with great consistency. Determining the efficiency of detection techniques is a challenge, especially when the study species are scarce or elusive. Such is the case with the American marten (Martes americana), a small mustelid found across the Northern United States and Canada. In the lower peninsula of Michigan, marten are considered a species of concern, and the full extent of their range remains unknown. My goal was to test the efficacy of motiontriggered cameras for detecting the presence of American …