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Full-Text Articles in Life Sciences

Using Noninvasive Calibrated Cuff Plethysmography To Observe The Effects Of Cold-Water Immersion On Arterial Compliance, Rita M. Grigorian Oct 2023

Using Noninvasive Calibrated Cuff Plethysmography To Observe The Effects Of Cold-Water Immersion On Arterial Compliance, Rita M. Grigorian

Master's Theses

As the prevalence of cardiovascular diseases continues to exponentially grow in populations across the globe, the necessity of determining underlying factors, effective methods of diagnoses, and universally available preventive measures also grows. Early detection of endothelial dysfunction, a proven precursor of cardiovascular diseases, can be extremely impactful in encouraging preventative measures and early intervention before medical conditions become chronic. In recent years, ice plunging, a form of cryotherapy involving full body immersion in cold water, has gained popularity within circles of fitness and health practitioners, gaining the interest of people of all backgrounds. Certain parallels observed between the human physiological …


Hydric Physiology Of Lizards, Savannah Weaver Jun 2023

Hydric Physiology Of Lizards, Savannah Weaver

Master's Theses

Chapter 1: Animals can respond to extreme climate by behaviorally avoiding it, or by physiologically coping with it. We understand behavioral thermoregulation and physiological thermal tolerances, but water balance has largely been neglected. Climate change includes both global warming and changes in precipitation regimes, so improving our understanding of organismal water balance is increasingly urgent. We assessed the hydric physiology of endangered Blunt-nosed Leopard Lizards (Gambelia sila) by measuring cutaneous evaporative water loss (CEWL), plasma osmolality, body mass, and body condition throughout their active season. On average, G. sila had low CEWL that is likely desert-adaptive, and high …


Southern Sea Otter (Enhydra Lutris Nereis) Fur Morphology, Thermal Function, And Buoyancy Across Ontogeny, Kate Riordan Jun 2023

Southern Sea Otter (Enhydra Lutris Nereis) Fur Morphology, Thermal Function, And Buoyancy Across Ontogeny, Kate Riordan

Master's Theses

Sea otters (Enhydra lutris) have the densest fur of any animal, and the hairs function to trap a layer of air that is used for insulation. When a sea otter is born, it has a natal pelage (baby fur), and sea otters eventually molt that fur and replace it with a pelt resembling the adult fur. Sea otter fur is composed of 3 types of hairs: underhairs, intermediate hairs, and guard hairs. Sea otters are more susceptible to the negative effects of oiling from oil spills compared to other marine mammal species because they solely rely on fur …


Effects Of Intertidal Position On The Response To Oxygen And Desiccation Stress In The Common Acorn Barnacle, Balanus Glandula, Megan M. Dotterweich Jun 2023

Effects Of Intertidal Position On The Response To Oxygen And Desiccation Stress In The Common Acorn Barnacle, Balanus Glandula, Megan M. Dotterweich

Master's Theses

Sessile invertebrates in the rocky intertidal experience intermittent periods of air exposure due to tidal flux, presenting risks of temperature extremes, hypoxia, nutrient limitation, and most dangerously, desiccation. Microscale variation in severity and frequency of these risks is widely dependent on vertical position within the intertidal zone. Common acorn barnacles (Balanus glandula) have a wide vertical distribution in the intertidal, creating large differences in microhabitat between the highest and lowest individuals in the population. This study set out to explore whether tidal position dependent differences exist in the response to oxygen and desiccation stress in B. glandula. …


Ankle Dorsiflexion Range Of Motion, Dynamic Balance, And Static Balance In Division Iii Collegiate Gymnasts, Emily Sabo May 2022

Ankle Dorsiflexion Range Of Motion, Dynamic Balance, And Static Balance In Division Iii Collegiate Gymnasts, Emily Sabo

Master's Theses

Introduction: Artistic gymnastics is a demanding sport composed of high intensity activities. Decreased range of motion can increase the risk of lower extremity injury due to the inability to disperse forces properly upon landing. Landing from a height demands dynamic balance, which may be influenced by ankle range of motion. Due to the complexity of the sport, identifying potential deficits at the ankle could lead to the development of injury prevention programs. Purpose: The purpose of this study is to investigate if correlations exist between active ankle dorsiflexion range of motion (AROM) and static balance and/or AROM and dynamic balance …


Habitat Heterogeneity Affects The Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Nicole Gaudenti Jun 2021

Habitat Heterogeneity Affects The Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Nicole Gaudenti

Master's Theses

Global climate change is already contributing to the extirpation of numerous species worldwide, and sensitive species will continue to face challenges associated with rising temperatures throughout this century and beyond. It is especially important to evaluate the thermal ecology of endangered ectotherm species now so that mitigation measures can be taken as early as possible. A recent study of the thermal ecology of the federally endangered Blunt-Nosed Leopard Lizard (Gambelia sila) suggested that they face major activity restrictions due to thermal constraints in their desert habitat, but that large shade-providing shrubs act as thermal buffers to allow them …


The Effects Of A Loaded Pre-Competition Activity Using Tethered Swimming On 25-Yard Swim Sprint Performance 2021, Jacob Burchfield May 2021

The Effects Of A Loaded Pre-Competition Activity Using Tethered Swimming On 25-Yard Swim Sprint Performance 2021, Jacob Burchfield

Master's Theses

Warmup is a standard practice for swim competitions, but there is minimal scientific evidence regarding the effectiveness of pre-competition activities (PCA) after the swim warmup. This study examined whether a loaded PCA using tethered swimming while attached to a power rack improves performance in a 25-yard swim sprint. Post activation performance enhancement (PAPE) may be the proposed mechanism for improvement resulting from a loaded PCA. To determine if PAPE existed following the loaded PCA, varsity swimmers from SUNY Cortland performed a 25-yard sprint a loaded PCA and after an unloaded PCA (control). All participants performed a standard meet warmup (1600-yards) …


Developing An Adaptive Rock Climbing Curriculum For Suny Cortland Outdoor Pursuits 2021, Rebekah Mills May 2021

Developing An Adaptive Rock Climbing Curriculum For Suny Cortland Outdoor Pursuits 2021, Rebekah Mills

Master's Theses

SUNY Cortland Outdoor Pursuits opened in August of 2015 with the completion of the newly constructed Student Life Center. Outdoor Pursuits is a smaller department within the Recreational Sports Department. Outdoor Pursuits offers rock climbing services at its facility located in the Student Life Center. Currently, Outdoor Pursuits offers Bouldering and Top Roping services only when the Student Life Center is open, these hours are typically 3-10 pm. Monday-Friday and Saturday-Sunday 2-8 pm. These services are only provided to eligible SUNY Cortland faculty, staff, students, alumni, and other affiliated members. As the facility has grown in popularity during its operation, …


Determinants Of The Carbon Footprint Of German Swimmers 2020., Ann-Marie Muehlbauer Aug 2020

Determinants Of The Carbon Footprint Of German Swimmers 2020., Ann-Marie Muehlbauer

Master's Theses

The current climate situation is placing our planet and future generations at risk, which demonstrates the urgency to combat climate change and conduct research to support this effort. Calculating the carbon footprint of active sport participation represents an important opportunity to conduct additional research. This study examined the environmental attitudes and practices of swim club members in Germany. The study had three goals: First, to calculate the total and partial carbon footprint of German swimmers; second, to understand how sport organizations induce participants to engage in pro-environmental behavior; and third, to analyze various factors associated with carbon footprint, such as …


An Investigation Of Stress-Related Factors And Injury Occurrence In Division Iii Ice Hockey Athletes 2020., Christian Kiesel Jun 2020

An Investigation Of Stress-Related Factors And Injury Occurrence In Division Iii Ice Hockey Athletes 2020., Christian Kiesel

Master's Theses

Many variables can factor into a student athlete sustaining an injury, but one of the most interesting is stress. The Stress and Injury Model is excellent in explaining how exactly history of stressors, personality, and coping mechanisms play a pivotal role in the stress response. If these three factors cause a negative stress response, then that will lead to an athlete becoming more likely to become injured. This study used 40 ice hockey players from a Division III NCAA institution. Each participant completed the Inventory of College Students’ Recent Life Experiences (ICSRLE) and Pittsburgh Sleep Quality Index (PSQI) every month …


Efficacy Of An Audio-Based Biofeedback Intervention To Modify Running Gait In Female Runners 2020, Sarah Rothstein May 2020

Efficacy Of An Audio-Based Biofeedback Intervention To Modify Running Gait In Female Runners 2020, Sarah Rothstein

Master's Theses

Introduction: Gait retraining interventions are used to modify foot strike parameters associated with musculoskeletal injuries. Such interventions may prove beneficial if gait modifications are maintained long-term and provide a physiological performance benefit. Purpose: The primary purpose of this study was to determine whether female recreational runners can use a smartphone decibel app to self-modify gait mechanics associated with injury. The secondary purpose was to determine if such gait modifications are retained beyond the initial training session. The tertiary purpose was to determine if such gait modifications were associated with improved running economy. Methods: The peak vertical ground reaction force (vGRF), …


The Effect Of Attentional Focus Instructions On Single Leg Balance Performance 2020., Cory Monahan May 2020

The Effect Of Attentional Focus Instructions On Single Leg Balance Performance 2020., Cory Monahan

Master's Theses

Balance and postural control exercises are often a part of exercise programs. During exercise programs, movement practitioners can provide instructions to facilitate performance and learning. Instructions can be used to direct attentional focus, which has been found to affect the performance and learning of motor skills, including balance and postural control tasks. However, no known studies to date have investigated the effect of both internal and external attentional focus instructions on static single leg balance performance. The purpose of this study was to investigate the effect of attentional focus instructions on static single leg balance performance as reflected by the …


A Comparison Of Power Variables For Two Loading Methods In Weighted Vertical Jumps 2020, Jonathan Tanguay Apr 2020

A Comparison Of Power Variables For Two Loading Methods In Weighted Vertical Jumps 2020, Jonathan Tanguay

Master's Theses

Training exercises to develop muscular strength and power, such as the weighted jump squat, commonly utilize barbells and dumbbells to increase resistance. Strength coaches often measure improvements in peak force, peak power, and rate of force development to determine effectiveness of a training program. The purpose of this study was to determine if equipment selection (barbells vs. dumbbells) affects peak force, peak power, and/or rate of force development in weighted jump squats. Thirteen physically active, college-aged males (age: 21.6 ± 2.0 years, height: 182.8 ± 9.7 cm, body mass: 87.2 ± 9.0 kg, lean mass: 72.3 ± 8.1 kg) performed …


Behavioral Ecology And Physiological Health Of Managed Populations Of Juvenile Blanding's Turtles (Emydoidea Blandingii) In Urban Wetlands, Armand Andre' Cann Jan 2020

Behavioral Ecology And Physiological Health Of Managed Populations Of Juvenile Blanding's Turtles (Emydoidea Blandingii) In Urban Wetlands, Armand Andre' Cann

Master's Theses

Substantial threats to reptile species biodiversity have become apparent in the last few decades. This is partly caused by significant losses in grasslands and their associate prairie-wetland ecosystems. One Midwestern prairie-wetland species, Blanding's Turtles (Emydoidea blandingii), are at risk of extirpation due to habitat loss, fragmentation, and increased predator populations. Consequently, significant head-starting efforts, a form of reintroduction conservation using captive-reared and then released turtles, have been conducted to recover populations of E. blandingii in northeastern Illinois. However, the success of these programs both locally and range-wide have not been fully quantified. Unfortunately, many approaches to guaging success involve long-term …


Effects Of Intertidal Position On Metabolism And Behavior In The Acorn Barnacle, Balanus Glandula, Kali Horn Nov 2019

Effects Of Intertidal Position On Metabolism And Behavior In The Acorn Barnacle, Balanus Glandula, Kali Horn

Master's Theses

The intertidal zone is characterized by persistent, tidally-driven fluctuations in both abiotic (e.g., temperature, [O2], salinity) and biotic (e.g., food availability, predation) conditions, which makes this a very physiologically challenging habitat for resident organisms. The magnitude and degree of variability of these environmental stressors differs between intertidal zones, with the most extreme physiological stress often being experienced by organisms in the high intertidal. Given that many of the fluctuating conditions in this environment are primary drivers of metabolic rate (e.g., temperature, [O2], food availability), we hypothesized that sessile conspecifics residing in different tidal zones would exhibit …


The Proteomic Response Of Northern Elephant Seal (Mirounga Angustirostris) Pups To Physiological Stress During Development, Melissa P. Voisinet Jun 2019

The Proteomic Response Of Northern Elephant Seal (Mirounga Angustirostris) Pups To Physiological Stress During Development, Melissa P. Voisinet

Master's Theses

Background: Northern elephant seals transition from terrestrial nursing pups to pelagic foraging juveniles in a short period of just 8-12 weeks. During the post-weaning period, pups rely solely on the energy reserves gained during nursing for their caloric demands and water supply. The prolonged absence of food after weaning is the first of many fasts for which the seals have evolved adaptations such as decreased urine production and increased blubber reserves. The stressors experienced from learning to dive for the first time are also stressors that they will experience frequently as an adult and for which they have evolved adaptations. …


The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli May 2019

The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli

Master's Theses

The spotted seatrout (Cynoscion nebulosus) is a euryhaline finfish candidate for marine aquaculture in the northern Gulf of Mexico. Aquaculture of this species currently uses salinities of 25 psu or greater making production challenging in areas where high salinity water is not available. This study aimed to document the effects of salinity on zootechnical performance of spotted seatrout embryos, early larvae, and juveniles in recirculating aquaculture systems to assist with the development of protocols for low salinity culture.

Embryos obtained from a volitional spawn at 29 psu were incubated at three salinities (12.5, 18.75, or 25 psu) until …


Comparative Thermal Ecology Of Coastal And Inland Pacific Rattlesnakes (Crotalus Oreganus), Hayley Layne Crowell May 2019

Comparative Thermal Ecology Of Coastal And Inland Pacific Rattlesnakes (Crotalus Oreganus), Hayley Layne Crowell

Master's Theses

Global biodiversity is declining as a direct result of anthropogenic climate change. Ectothermic species have become focal organisms for studying the ecological effects of altered climates due to the clear relationship between environmental temperatures and ectotherms’ basic physiological functions. Historically, examinations of these effects have focused heavily on heliothermic lizards, and most others have tended to focus on single populations or sympatric species within a single community. Addressing the longterm energetic implications of environmental temperature variation will provide valuable insight into the cascading physiological effects that certain populations or species may experience as a result of altered climates.

In this …


Adolescent Binge Alcohol Exposure Effects On Heart Structure And Function, Lizhuo Ai Jan 2019

Adolescent Binge Alcohol Exposure Effects On Heart Structure And Function, Lizhuo Ai

Master's Theses

More than 5 million underage Americans report binge drinking at least once per month. While the effects of alcohol consumption on the adult heart have been well studied, the impact to the adolescent heart is almost entirely unknown. Adolescents primarily consume alcohol in a binge pattern, which elevates blood alcohol content (BAC) to 0.08 g/dL within 2 hours. During adolescence the body grows rapidly, and the heart must also grow by cellular hypertrophy to meet this increasing demand. Our goal was to determine the impact of adolescent binge alcohol exposure on the heart, using an outbred rat model. This thesis …


Greening Rates And Photosynthetic Development Of Leaves In C3 And C4 Plants, Tayler J. Kriss Jan 2019

Greening Rates And Photosynthetic Development Of Leaves In C3 And C4 Plants, Tayler J. Kriss

Master's Theses

To study chlorophyll development time and overall photosynthetic development in C3 and C4 leaves, seeds were germinated in complete darkness and achlorophyllous leaves were then allowed to develop in lighted conditions. Corn (Zea mays, C4), sorghum (Sorghum bicolor, C4), green bean (Phaseolus vulgaris, C3), broad bean (Vicia faba, C3), and wheat (Triticum aestivum, C3) were investigated for the first ten days of sunlight exposure. Chlorophyll concentration, chlorophyll fluorescence, and CO2 gas exchange measurements were conducted daily on the first leaf that emerged after the embryonic leaves of each plant. The first five days of the experiment, days zero to four …


The Thermal Ecology Of Sceloporus Occidentalis, Luis Patricio Burgos Dec 2018

The Thermal Ecology Of Sceloporus Occidentalis, Luis Patricio Burgos

Master's Theses

With temperatures rising globally, assessing the possible impacts of the changing climate becomes more and more urgent. Ectotherms are excellent indicators of potential climatic ramifications on biodiversity because of their heavy reliance on the environment for their thermoregulation. Studies have historically looked at thermal tolerance values to establish predictive models for population and species extinctions.

In chapter 1, we looked at recent studies that suggest that thermal tolerance may be a plastic trait and test the effects empirically. Most studies are based on captive lizards acclimated to laboratory conditions that do not necessarily reflect natural environments, and if thermal tolerance …


Physiological Response Of The Giant Acorn Barnacle, Balanus Nubilus, To Air Exposure, Emily Jane Resner Nov 2018

Physiological Response Of The Giant Acorn Barnacle, Balanus Nubilus, To Air Exposure, Emily Jane Resner

Master's Theses

The giant acorn barnacle, Balanus nubilus, is a resident of the subtidal and low intertidal rocky shoreline on the Pacific Coast of North America (Alaska to Baja California). B. nubilusis notable for having the largest muscle fibers in the animal kingdom; fiber diameters that can exceed 3mm in adults! At such extreme sizes these muscle cells may be at risk for insufficient oxygen delivery to mitochondria owing to low SA:V ratios and long intracellular diffusion distances. Oxygen limitation to these muscles may be further exacerbated during low tide air exposure (emersion) or environmental hypoxia events, which are increasing …


The Effects Of A Low Dose Of Caffeine On Bat Swing Performance In Fatigued And Non-Fatigued Female Softball Athletes 2018, Brianna Ferchen May 2018

The Effects Of A Low Dose Of Caffeine On Bat Swing Performance In Fatigued And Non-Fatigued Female Softball Athletes 2018, Brianna Ferchen

Master's Theses

Objective: The purpose of this study was to determine if caffeine ingestion decreased time to contact (TTC), and increased peak hand speed (HS), peak bat barrel speed (BS), and power (P) during a softball bat swing by fatigued and non-fatigued female athletes from an NCAA Division III varsity softball team. Methods: A randomized, single blind counterbalanced design was used to determine if 200 mg of caffeinated gum produced an ergogenic effect on nine female softball players (mean ± SD; age: 19.4 ± 0.7 yrs; height: 169 ± 6 cm; weight: 76.85 ± 10.82 kg) during a softball swing after chewing …


The Effectiveness Of Men's Lacrosse Shoulder Pads In The Attenuation Of Linear Impact Forces 2018, Ryan S. Dambach May 2018

The Effectiveness Of Men's Lacrosse Shoulder Pads In The Attenuation Of Linear Impact Forces 2018, Ryan S. Dambach

Master's Theses

BACKGROUND/PURPOSE: Men’s lacrosse is a sport that is growing in popularity. The mandatory shoulder pads have no limit of use standards and are rarely studied. The purpose of the study was to measure peak impact force (IF) of new and used lacrosse shoulder pads. METHODS: Six lacrosse shoulder pads, three new models (Warrior MPG 8.0 shoulder pad, STX Jolt shoulder pad and Warrior Player’s Club Hitlyte shoulder pad) and three used models of the same shoulder pads with at least four seasons of use (Warrior MPG shoulder pad, STX Jolt shoulder pad and Warrior Player’s Club Hitlyte shoulder pad) were …


The Relationship Between Stryd Power And Running Economy In Well-Trained Distance Runners 2018, Casey Austin May 2018

The Relationship Between Stryd Power And Running Economy In Well-Trained Distance Runners 2018, Casey Austin

Master's Theses

A novel running wearable called the Stryd Summit footpod attaches to a runner’s right or left shoe and measures running power output. The developers of the product purport that the footpod’s power and form power measures may correlate with metabolic data gathered in a lab. PURPOSE: Explore the relationship between power output and running economy at threshold pace. METHODS: Seventeen well-trained distance runners, 9 males and 8 females, completed a running protocol at threshold pace. Participants ran two discontinuous four-minute stages: one with their self-selected cadence (SS), and one with cadence lowered by 10% (LC). Metabolic data, power, and form …


Effects Of Competition Variables And Prior Attempts On Approach Run Velocity During A Pole Vaulter's Final Clearance 2018, Nathaniel S. Ashton May 2018

Effects Of Competition Variables And Prior Attempts On Approach Run Velocity During A Pole Vaulter's Final Clearance 2018, Nathaniel S. Ashton

Master's Theses

Approach run velocity of a vaulter is strongly correlated to the highest height a vaulter clears in pole vault competition and the number of attempts taken throughout a competition influences pole vault strategy. Since approach run velocity greatly affects the crossbar height cleared and number of attempts affects time spent in the competition, perhaps a better approach to determine optimal competition strategy is to first identify how competition variables influence approach run velocity. The purpose of this study was to determine if the approach run velocity during a pole vaulter’s last clearance can be predicted by: (1) the number of …


Learning To Run On An Lbppt: Neuromuscular And Metabolic Adaptations 2018, Jordyn Naylon May 2018

Learning To Run On An Lbppt: Neuromuscular And Metabolic Adaptations 2018, Jordyn Naylon

Master's Theses

Previous research on lower body positive pressure (LBPP) treadmills have focused primarily on muscle activation (EMG), spatiotemporal variables, and metabolic differences from running on a standard treadmill. The current study aimed to establish the necessary accommodation time needed for runners to display reliable metabolic and neuromuscular responses on the LBPP treadmill. Fifteen habitually trained runners (11 males, 4 females, average± SD VO2Max=62.81 ± 5.43mL.kg-1.min-1) ran a total of 3, 15-minute trials on the AlterG® treadmill during a single testing session. For each trial, the AlterG® was set to support 30% of body weight at 70% of the speed at which …


The Effects Of Dietary Nitrate Supplementation On Swimming Performance In Collegiate Swimmers 2018, Christopher Daniel Mosconi May 2018

The Effects Of Dietary Nitrate Supplementation On Swimming Performance In Collegiate Swimmers 2018, Christopher Daniel Mosconi

Master's Theses

The desire for performance enhancement is a common goal amongst athletes. Recently a number of studies have reported various performance enhancing effects of dietary nitrate (NO3-) via beetroot juice (BRJ) supplementation. Research has indicated that the various performance enhancing effects of BRJ can be attributed to a decrease resting systolic blood pressure (SBP), diastolic blood pressure (DBP), and submaximal volume of oxygen consumption (VO2), while increasing vasodilation and mitochondrial efficiency. Purpose: The purpose of this study was to investigate whether BRJ supplementation improves swimming performance in collegiate level swimmers. Methods: Eight collegiate trained swimmers underwent a double-blind 5-day chronic supplementation …


Relationships Among Actual Motor Competence, Perceived Motor Competence And Health-Related Fitness In A College-Aged Population 2018, Samantha Moss May 2018

Relationships Among Actual Motor Competence, Perceived Motor Competence And Health-Related Fitness In A College-Aged Population 2018, Samantha Moss

Master's Theses

Purpose: The purpose of this study was to assess relationships among actual motor competence, perceived motor competence, and health-related fitness in a college-aged population. Methods: A total of 76 participants from SUNY Cortland enrolled in an undergraduate Kinesiology course completed an informed consent form. Total data were obtained on 71 participants (male = 53; female = 18). Perceived motor competence (PMC) was assessed via the Physical Self Perception Profile questionnaire, which participants completed one week prior to remaining assessments. Motor competence (MC) was assessed by maximum throw and kick speed as well as maximum distance jumped. Health-related fitness (HRF) was …


The Effects Of A Short-Term, Unilateral, Lower-Body Resistance Training Program On Balance In College-Aged Resistance-Trained Participants 2018., Daniel Semprini May 2018

The Effects Of A Short-Term, Unilateral, Lower-Body Resistance Training Program On Balance In College-Aged Resistance-Trained Participants 2018., Daniel Semprini

Master's Theses

Purpose: The purpose of this study was to investigate whether a short-term, unilateral, lower-body resistance training program would significantly improve static and dynamic balance in experienced college-aged resistance-trained participants when compared to a control group’s regular, bilateral lower-body resistance training program. Participants: A total of twenty-four participants were recruited to participate in the study. Four participants ended up dropping out due to injury and time constraints, leaving the final total sample size at twenty. Methods: Participants completed a series of three questionnaires (International Fitness Scale, International Physical Activity, and Sociodemographic Questionnaire) and the informed consent. The participants were randomly divided …