Life Sciences Commons^™

Automated Tree Mortality Detection Using Ubiquitously Available Public Data, Michael T. Huggins

Master's Theses

Understanding the dynamic interplay between fire severity, topography, and tree mortality, is crucial for predicting future forest dynamics and enhancing resilience against climate change-induced wildfire regimes. This thesis develops a multi-sensor approach for automated estimation of tree mortality, then applies it to examine trends in tree mortality over a six-year period across a fire affected study site in the Trinity River basin in Northern California. The Random Forest model uses publicly available USGS 3D Elevation Program Lidar (3DEP) and NAIP imagery as inputs and is likely to be easily adaptable to other landscapes. The model had a Receiver Operating Characteristic …

Covalent Inhibition Of Enzyme Sortase A As A New Pathway Against Bacterial Resistance Of Staphylococcus Aureus, Umyeena Bashir

Master's Theses

The continued rise of antibiotic-resistant bacteria, such as MRSA (methicillin-resistant Staphylococcus aureus), has made the discovery of novel antibiotics critical. While bacteriostatic and bactericidal antibiotics inevitably exert evolutionary pressure on bacteria to develop resistance, small molecules that target mechanisms of bacterial virulence present a promising alternative for treatment. Sortase A (Srt A), a cysteine protease of S. aureus, promotes bacterial virulence by covalently attaching proteins such as pilin to the bacterial surface, enabling bacterial adhesion to mammalian cells. Inhibitory studies of this enzyme have gained prominent interest as a new pathway for drug development since blocking this enzyme's ability to …

Annotation Of Non-Model Species’ Genomes, Taiya Jarva

Master's Theses

The innovations in high throughput sequencing technologies in recent decades has allowed unprecedented examination and characterization of the genetic make-up of both model and non-model species, which has led to a surge in the use of genomics in fields which were previously considered unfeasible. These advances have greatly expanded the realm of possibilities in the fields of ecology and conservation. It is now possible to the identification of large cohorts of genetic markers, including single nucleotide polymorphisms (SNPs) and larger structural variants, as well as signatures of selection and local adaptation. Markers can be used to identify species, define population …

Solving The Cable Equation, A Second-Order Time Dependent Pde For Non-Ideal Cables With Action Potentials In The Mammalian Brain Using Kss Methods, Nirmohi Charbe

Master's Theses

In this thesis we shall perform the comparisons of a Krylov Subspace Spectral method with Forward Euler, Backward Euler and Crank-Nicolson to solve the Cable Equation. The Cable Equation measures action potentials in axons in a mammalian brain treated as an ideal cable in the first part of the study. We shall subject this problem to the further assumption of a non-ideal cable. Assume a non-uniform cross section area along the longitudinal axis. At the present time, the effects of torsion, curvature and material capacitance are ignored. There is particular interest to generalize the application of the PDEs including and …

Predicting Location And Training Effectiveness (Plate), Erik Rolf Bruenner

Master's Theses

Abstract Predicting Location and Training Effectiveness (PLATE)
Erik Bruenner

Physical activity and exercise have been shown to have an enormous impact on many areas of human health and can reduce the risk of many chronic diseases. In order to better understand how exercise may affect the body, current kinesiology studies are designed to track human movements over large intervals of time. Procedures used in these studies provide a way for researchers to quantify an individual’s activity level over time, along with tracking various types of activities that individuals may engage in. Movement data of research subjects is often collected through …

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Rattus Norvegicus As A Biological Detector Of Clandestine Remains And The Use Of Ultrasonic Vocalizations As A Locating Mechanism, Gabrielle M. Johnston

Master's Theses

In investigations, locating missing persons and clandestine remains are imperative. One way that first responder and police agencies can search for the remains is by using cadaver dogs as biological detectors. Cadaver dogs are typically used due to their olfactory sensitivity and ability to detect low concentrations of volatile organic compounds produced by biological remains. Cadaver dogs are typically chosen for their stamina, agility, and olfactory sensitivity. However, what is not taken into account often is the size of the animal and the expense of maintaining and training the animal. Cadaver dogs are typically large breeds that cannot fit in …

Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis

Master's Theses

This study aspires to find a new screening approach to trace DNA recovery techniques to yield a higher quantity of trace DNA from larger items of evidence. It takes the path of visualizing trace DNA on items of evidence with potential DNA so analysts can swab a more localized area rather than attempting to recover trace DNA through the general swabbing technique currently used for trace DNA recovery. The first and second parts consisted of observing trace DNA interaction with Diamond Dye on porous and non-porous surfaces.

The third part involved applying the Diamond Dye solution by spraying it onto …

Psf Sampling In Fluorescence Image Deconvolution, Eric A. Inman

Master's Theses

All microscope imaging is largely affected by inherent resolution limitations because of out-of-focus light and diffraction effects. The traditional approach to restoring the image resolution is to use a deconvolution algorithm to “invert” the effect of convolving the volume with the point spread function. However, these algorithms fall short in several areas such as noise amplification and stopping criterion. In this paper, we try to reconstruct an explicit volumetric representation of the fluorescence density in the sample and fit a neural network to the target z-stack to properly minimize a reconstruction cost function for an optimal result. Additionally, we do …

The Anatomy And Phylogeny Of A New Large Plioplatecarpine Mosasaur From The Campanian Bearpaw Shale Of Montana (Usa), Richard A. Carr

Master's Theses

In 2018, a large and associated plioplatecarpine mosasaur skull, pectoral girdle, and rib cage, whose total body length may have exceeded five meters, was uncovered in the Late Campanian Bearpaw Shale of Northeast Montana (USA). Phylogenetic analysis of this specimen, MOR 10855, recovers this individual as a basal member of the genus Plioplatecarpus. This specimen, is unique in that it is estimated to be nearly twice the size of any of the other species of Plioplatecarpus found in the Western Interior Seaway during this part of the Cretaceous. While the included phylogenetic study suggests MOR 10855 represents a new …

Timing Of Diversification, Dispersal, And Biogeography Of Parrots In The Genus Amazona (Psittaciformes: Psittacidae) Throughout The Caribbean, Visualized In Gis, Christopher Kingwill

Master's Theses

Avian fossil records from across the Caribbean (Greater and Lesser Antilles) demonstrate higher avian diversity prior to extinction events due to climate change at the end of the Pleistocene and human impact across the Caribbean throughout the Holocene. Amazon parrots (Amazona) are a diverse genus of New World parrots found throughout Central and South America, as well as the Caribbean. Their phylogeny and evolutionary history, specifically for Caribbean species, has been debated in terms of source areas in Central and South America and the timing of and number of colonization events to different islands that preceded diversification into …

Investigation Of Dead Ocean Quahogs (Arctica Islandica) Shells On The Mid-Atlantic Bight Continental Shelf, Alyssa Leclaire

Master's Theses

Ocean quahogs, Arctica islandica, are a long-lived, widely dispersed, biomass dominate in the Mid-Atlantic; therefore, quahog shells are valuable resources for studying climate change over time. Recently, dead ocean quahog shells were discovered south and inshore of the present biogeographic range of this animal. The presence of ocean quahog shells outside the current range is presumably a consequence of past regressions and transgressions of the Cold Pool, the bottom-trapped, cool body of water that allows boreal animals to live at lower latitudes. Dead ocean quahog shells were collected offshore of the DelMarVa Peninsula then radiocarbon-dated, evaluated for taphonomic condition, …

A Comparison Of Natural, Living, And Hardened Shorelines Ability To Prevent Coastal Erosion And Maintain A Healthy Ecosystem, Gabrielle Spellmann

Master's Theses

It is important to find a suitable method to protect the U.S. Gulf Coast shoreline, since its’ low elevation and the Loop current make it vulnerable to sea level rise. I focused on two manmade methods, hardened, and living shorelines, of coastal protection for when the natural marsh suffers excess erosion rates. Living shorelines are a suite of shoreline conservation and restoration techniques that usually involve some sort of hardened structure that dampens wave energy so that the native vegetation behind it can take root and stabilize the shoreline. This study looked at six different sites, all containing a natural, …

Climate Adaptation And Water Conservation Decision-Making In Paso Robles, California Vineyards, Christopher R. Klier

Master's Theses

This paper examines vineyard perceptions and adoption of climate change adaptation and water conservation measures in the Paso Robles American Viticultural Area (AVA). A survey was distributed to all 220 vineyards and vineyard management companies that operate in the AVA, with a 53.64% response rate. The objective of the survey was to determine vineyard manager and owner attitudes towards climate change and adaptation, as well as their perceptions of how these threats would impact their operation. A second objective was to document the current level of water conservation and climate adaptation while identifying the barriers and opportunities for further adoption …

Polycyclic Aromatic Hydrocarbons And The Microbiomes Of Two Benthic Species, Samantha Ells

Master's Theses

The presence of oil has been shown to affect the microbiomes of the water column, sediments, and organisms, both by altering the diversity and the composition of those microbial communities. If the microbiome is altered it may no longer provide benefits to its host organism, impacting its ability to survive. Thus, it is important to understand the effects of large-scale contamination events including the Deepwater Horizon oil spill. This study set out to examine the effects of oil exposure on the microbiome of two benthic species, southern flounder (Paralichthys lethostigma) and eastern oysters (Crassostrea virginica) to further understand the effects …

Analysis Of The Slo Bay Microbiome From A Network Perspective, Lien Viet Nguyen

Master's Theses

Microorganisms are key players in the ecosystem functioning. In this thesis, we developed a framework to preprocess raw microbiome data, build a correlation network, and analyze co-occurrence patterns between microbes. We then applied this framework to a marine microbiome dataset. The dataset used in this study comes from a year-long time-series to characterize the microbial communities in our coastal waters off the Cal Poly Pier. In analyzing this dataset, we were able to observe and confirm previously discovered patterns of interactions and generate hypotheses about new patterns. The analysis of co-occurrences between prokaryotic and eukaryotic taxa is relatively novel and …

Ecological Controls On The Campanian Distribution Of Hesperornis (Aves: Hesperornithiformes) In The Western Interior Seaway, Blake Chapman

Master's Theses

The epicontinental Western Interior Seaway (WIS) of Late Cretaceous North America provided a unique marine habitat for cephalopods, fish, marine reptiles, and the foot-propelled diving seabird Hesperornis. While several predator-prey relationships among Hesperornis or other hesperornithiforms and other WIS animals have been hypothesized based on gut contents, bite marks, and coprolites/colonites, ecological relationships have not been quantitatively tested. Paleontological species distribution modeling (SDM) studies have focused on extinct non-marine taxa and marine invertebrates, with only two marine vertebrate studies of extant taxa. Here, two SDM methods were used to test the influence of vertebrate faunas, sedimentary rock type, paleogeography, …

An Investigation Into Historical And Contemporary Breeding Occurrence Of The Ferruginous Hawk In Kansas, Erica Clark

Master's Theses

The Ferruginous Hawk (Buteo regalis) is the largest Buteo species occurring in grasslands, nesting along bluffs, buttes and isolated trees. In Kansas, the Ferruginous Hawk is listed as a Species of Greatest Conservation Need, Tier II. A previous study on Ferruginous Hawks nesting in Kansas during the years of 1979 to 1987, with sporadic visits from the 1990s to 2000, revealed that the most productive nesting territories were inaccessible to predators, placed on rocky ledges and the surrounding landscape was over 50% rangeland. I revisited 82 of the 111 historic nest territories in the summers of 2019 and …

Eelgrass (Zostera Marina) Population Decline In Morro Bay, Ca: A Meta-Analysis Of Herbicide Application In San Luis Obispo County And Morro Bay Watershed, Tyler King Sinnott

Master's Theses

The endemic eelgrass (Zostera marina) community of Morro Bay Estuary, located on the central coast of California, has experienced an estimated decline of 95% in occupied area (reduction of 344 acres to 20 acres) from 2008 to 2017 for reasons that are not yet definitively clear. One possible driver of degradation that has yet to be investigated is the role of herbicides from agricultural fields in the watershed that feeds into the estuary. Thus, the primary research goal of this project was to better understand temporal and spatial trends of herbicide use within the context of San Luis …

Determining The Trophic Role Of Red Snapper (Lutjanus Campechanus) In Mississippi State Waters Using Stomach Content And Stable Isotope Analysis, Branden Kohler

Master's Theses

The goal of this study was to determine the diet composition, trophic position and ecological role of red snapper (Lutjanus campechanus) in Mississippi state waters utilizing stable isotopes (δ¹³C and δ¹⁵N) and stomach content analysis. Stable isotope analysis of fish and their prey can provide information on species-specific basal resource utilization, diet composition and trophic position which can improve food web models and inform fisheries management decisions. Particulate organic matter (POM), the presumed base of the food web, red snapper muscle tissue, and red snapper stomach contents were collected from 25 sites in 2016 and …

Sensory Stressors Impact Species Responses Across Local And Continental Scales, Ashley A. Wilson

Master's Theses

Pervasive growth in industrialization and advances in technology now exposes much of the world to anthropogenic night light and noise (ANLN), which pose a global environmental challenge in terrestrial environments. An estimated one-tenth of the planet’s land area experiences artificial light at night — and that rises to 23% if skyglow is included. Moreover, anthropogenic noise is associated with urban development and transportation networks, as the ecological impact of roads alone is estimated to affect one-fifth of the total land cover of the United States and is increasing in space and intensity. Existing research involving impacts of light or noise …

Pressure Driven Desalination Utilizing Nanomaterials, Fangyou Xie

Master's Theses

Nanomaterials such as graphene oxide and carbon nanotubes, have demonstrated excellent properties for membrane desalination, including decrease of maintenance, increase of flux rate, simple solution casting, and impressive chemical inertness. Here, two projects are studied to investigate nanocarbon based membrane desalination. The first project is to prepare hybrid membranes with amyloid fibrils intercalated with graphene oxide sheets. The addition of protein amyloid fibrils expands the interlayer spacing between graphene oxide nanosheets and introduces additional functional groups in the diffusion pathways, resulting in increase of flux rate and rejection rate for the organic dyes. Amyloid fibrils also provide structural assistance to …

Determinants Of The Carbon Footprint Of German Swimmers 2020., Ann-Marie Muehlbauer

Master's Theses

The current climate situation is placing our planet and future generations at risk, which demonstrates the urgency to combat climate change and conduct research to support this effort. Calculating the carbon footprint of active sport participation represents an important opportunity to conduct additional research. This study examined the environmental attitudes and practices of swim club members in Germany. The study had three goals: First, to calculate the total and partial carbon footprint of German swimmers; second, to understand how sport organizations induce participants to engage in pro-environmental behavior; and third, to analyze various factors associated with carbon footprint, such as …

Analysis Of Biofilm Remediation Capacity For Octenyl Succinic Anhydride (Osa), A Bioactive Food Starch Modifier Compound, Matthew R. Borglin

Master's Theses

Matthew R. Borglin

This thesis demonstrates efficacy of Octenyl Succinic Anhydride (OSA), as a biofilm sanitizer. Biofilms allow bacteria to adhere to solid surfaces with the use of excreted polymeric compounds. For example, surfaces found in food production or processing facilities such as the interior of a raw milk holding tank, are some of the most susceptible to biofilm contamination. When present, biofilms can cause a variety of negative effects, which include; reduction of product shelf life, corrosion, and outbreaks of foodborne illnesses. The close association of biofilms with the majority of foodborne illness cases led the US Environmental Protection …

Cal Poly Pier Master Plan, Troy A. Lawson

Master's Theses

The Cal Poly Pier (Pier) Master/Facility Plan (FP) document provides the vision of the future for the Pier, a marine science research facility. The Plan facilitates project development and management of the Pier while meeting university and department research goals. Specifically, the FP document establishes goals and strategies to direct long-term development of the Pier, streamlines agency approval and permit requirements, provides context for pier management, and assists the permitting process for future development as it relates to regulatory permits and programmatic growth on the Cal Poly Pier to help meet goals of the Center for Coastal Marine Sciences (CCMS). …

An Analysis Of Dredge Efficiency For Surfclam And Ocean Quahog Commercial Dredges, Leanne Poussard

Master's Theses

Between 1997 and 2011, The National Marine Fisheries Service conducted 50 depletion experiments to estimate survey gear efficiency and stock density for Atlantic surfclam (Spisula solidissima) and ocean quahog (Arctica islandica) populations using commercial hydraulic dredges. The Patch Model was formulated to estimate gear efficiency and organism density from the data. The range of efficiencies estimated is substantial, leading to uncertainty in the application of these estimates in stock assessment. Analysis of depletion experiment simulations showed that uncertainty in the estimates of gear efficiency from depletion experiments was reduced by higher numbers of dredge tows per experiment, more tow overlap …

Valorization Of Carrot Processing Waste, Alexandra M. Duval

Master's Theses

Commercial carrot processors produce up to 175,000 tons of carrot waste annually. Carrot Mash (CM) is the term referring to the waste by-product of peeled baby carrot processing. Transportation of carrot processing waste is expensive due to its high- water content (approx. 83-95%). High in bioactive compounds (carotenoids) and dietary fibers, it is expected that its conversion into a value-added by-product is of interest to the carrot processing industry. Hemicellulose-rich plant materials have proven to be a source of oligosaccharides, which are known for their beneficial prebiotic activity. The objectives of this research were to: 1) determine the effect of …

An Assessment Of Convergence In The Feeding Morphology Of Xiphactinus Audax And Megalops Atlanticus Using Landmark-Based Geometric Morphometrics, Edward Chase Shelburne

Master's Theses

Convergence is an evolutionary phenomenon wherein distantly related organisms independently develop features or functional adaptations to overcome similar environmental constraints. Historically, convergence among organisms has been speculated or asserted with little rigorous or quantitative investigation. More recent advancements in systematics has allowed for the detection and study of convergence in a phylogenetic context, but this does little to elucidate convergent anatomical features in extinct taxa with poorly understood evolutionary histories. The purpose of this study is to investigate one potentially convergent system—the feeding structure of Xiphactinus audax (Teleostei: Ichthyodectiformes) and Megalops atlanticus (Teleostei: Elopiformes)—using a comparative anatomical approach to assess …

Effects Of Water Table Depth And Edaphic Characteristics On Plant Diversity In A Southern Mississippi Pitcher Plant Bog, Patrick Kirby

Master's Theses

This study examined the effects that water table depth and soil characteristics have on plant species richness and species composition within pitcher plant bogs across seasons. Eight piezometers were installed at random distances to monitor long-term water table depth and pressure fluctuations along a ~710-meter line transect traversing upland and bog habitats. Vegetation sampling quadrats (n=128) were set up near each piezometer. Cover data and water table depths were collected in spring and late summer. Soil samples collected from each treatment group were used to obtain soil texture and nutrient data. The summer collection period yielded a total gdiversity of …

Carbonate Chemistry Characterization In A Low-Inflow Estuary With Recent Seagrass Loss, Jolie Higgins

Master's Theses

Estuaries are dynamic environments that are strongly affected by natural variability, as well as direct and indirect anthropogenic impacts. A better understanding of the drivers of carbon fluxes and biogeochemical variability in estuarine systems is needed, particularly with the increasing threat of ocean acidification. Morro Bay in Central California is a small nationally protected estuary, with seasonally low freshwater inputs. Since 2007, the bay has experienced a significant loss of native seagrass, Zostera marina, which is an important component of the marine ecosystem. Because seagrass photosynthesis decreases carbon dioxide and increases oxygen in the water column, the loss of seagrass …

A Machine Learning Technology For Rapid Detection Of Carbon Nanotubes/Dna Hybridization In Biosensor Healthcare Applications, Steven K. Ang

Master's Theses

In molecular biology, the term “DNA hybridization” generally refers to the process of forming a double stranded nucleic acid from joining two complementary strands of DNA. The degree of genetic similarity of the DNA resulting from hybridization can be detected ei ther by using the chemical characteristics of DNA samples or by utilizing reliable biosensors which transform the chemical characteristics into a source of electrical measurements. In past research about such sensors, known as DNA Hybridization Detection Systems, the thermal and electrical characteristics of carbon nanotubes are utilized to detect whether hybridization takes place or not. However, human interpretation of …