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Full-Text Articles in Life Sciences
Msh1-Mediated Non-Genetic Phenotypic Variation In Plants: Prospects For Epigenetic Breeding In Sorghum Bicolor, Dikungwa Ketumile
Msh1-Mediated Non-Genetic Phenotypic Variation In Plants: Prospects For Epigenetic Breeding In Sorghum Bicolor, Dikungwa Ketumile
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Sorghum is the fifth most important cereal crop grown in the world, and is the primary staple food for many people in developing countries. Like other major crops, its improvement is constrained by depletion of genetic diversity within breeding germplasm. Investigated in this study is the unique creation of non-genetic phenotypic variation through altering MutS Homolog1 (MSH1), a plant-specific gene, and the potential it presents for sorghum improvement. Suppression of MSH1 results in non-genetic developmental reprogramming. The derived MSH1 memory lines, when used in crossing, result in heritable phenotypic variation that enhances plant vigor and agronomic performance. A …
A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk
A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk
Department of Agronomy and Horticulture: Faculty Publications
Identifying and characterizing alternative splicing (AS) enables our understanding of the biological role of transcript isoform diversity. This study describes the use of publicly available RNA-Seq data to identify and characterize the global diversity of AS isoforms in maize using the inbred lines B73 and Mo17, and a related species, sorghum. Identification and characterization of AS within maize tissues revealed that genes expressed in seed exhibit the largest differential AS relative to other tissues examined. Additionally, differences in AS between the two genotypes B73 and Mo17 are greatest within genes expressed in seed. We demonstrate that changes in the level …
Insect And Plant-Derived Mirnas In Greenbug (Schizaphis Graminum) And Yellow Sugarcane Aphid (Sipha Flava) Revealed By Deep Sequencing, Haichuan Wang, Chi Zhang, Yongchao Dou, Bin Yu, Yunfeng Liu, Tiffany Heng-Moss, Guoqing Lu, Michael Wachholtz, Jeffery D. Bradshaw, Paul Twigg, Erin Scully, Nathan Palmer, Gautam Sarath
Insect And Plant-Derived Mirnas In Greenbug (Schizaphis Graminum) And Yellow Sugarcane Aphid (Sipha Flava) Revealed By Deep Sequencing, Haichuan Wang, Chi Zhang, Yongchao Dou, Bin Yu, Yunfeng Liu, Tiffany Heng-Moss, Guoqing Lu, Michael Wachholtz, Jeffery D. Bradshaw, Paul Twigg, Erin Scully, Nathan Palmer, Gautam Sarath
Department of Entomology: Faculty Publications
Schizaphis graminum (green bug; GB) and Sipha flava (yellowsugarcane aphid; YSA) are two cereal aphid species with broad host ranges capable of establishing on sorghum (Sorghum bicolor) and several switchgrass (Panicum virgatum) cultivars. Switchgrass and sorghum are staple renewable bioenergy crops that are vulnerable to damage by aphids, therefore, identifying novel targets to control aphids has the potential to drastically improve yields and reduce losses in these bioenergy crops. Despite thewealth of genomic and transcriptomic information available from a closely related model aphid species, the pea aphid (Acyrthosiphon pisum), similar genomic information, including the …
Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente
Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente
Department of Agronomy and Horticulture: Faculty Publications
Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire and assimilate nitrogen more efficiently is a key agronomic parameter that will augment sustainability in agriculture. A transcription factor approach was pursued to address improvement of nitrogen use efficiency in two major commodity crops. To this end, the Zea mays Dof1 (ZmDof1) transcription factor was expressed in both wheat (Triticum aestivum) and sorghum (Sorghum bicolor) either constitutively, UBI4 promoter from sugarcane, or in a tissue specific fashion via the maize rbcS1 promoter. The primary transcription activation target of ZmDof1 …
Validation Of Qtl Mapping And Transcriptome Profiling For Identification Of Candidate Genes Associated With Nitrogen Stress Tolerance In Sorghum, Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E. Clemente, David R. Holding, Ismail M. Dweikat
Validation Of Qtl Mapping And Transcriptome Profiling For Identification Of Candidate Genes Associated With Nitrogen Stress Tolerance In Sorghum, Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E. Clemente, David R. Holding, Ismail M. Dweikat
Department of Agronomy and Horticulture: Faculty Publications
Background: Quantitative trait loci (QTLs) detected in one mapping population may not be detected in other mapping populations at all the time. Therefore, before being used for marker assisted breeding, QTLs need to be validated in different environments and/or genetic backgrounds to rule out statistical anomalies. In this regard, we mapped the QTLs controlling various agronomic traits in a recombinant inbred line (RIL) population in response to Nitrogen (N) stress and validated these with the reported QTLs in our earlier study to find the stable and consistent QTLs across populations. Also, with Illumina RNA-sequencing we checked the differential expression of …