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University of Nebraska - Lincoln

2008

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Articles 1 - 30 of 991

Full-Text Articles in Life Sciences

Integrated Management Strategies For Phytophthora Sojae Combining Host Resistance And Seed Treatments, A. E. Dorrance, A. E. Robertson, S. Cianzo, L. J. Giesler, C. R. Grau, M. A. Draper, A. U. Tenuta, T. R. Anderson Dec 2008

Integrated Management Strategies For Phytophthora Sojae Combining Host Resistance And Seed Treatments, A. E. Dorrance, A. E. Robertson, S. Cianzo, L. J. Giesler, C. R. Grau, M. A. Draper, A. U. Tenuta, T. R. Anderson

Department of Plant Pathology: Faculty Publications

Phytophthora sojae has re-emerged as a serious soybean pathogen in the past decade. This may be due in part to changes in resistance levels in current cultivars, adoption of P. sojae populations to deployed Rps genes, and highly favorable environments in the past decade. This multilocation study evaluated the effect of seed treatments on the incidence and severity of Phytophthora root and stem rot on soybeans with different combinations of Rps genes and levels of partial resistance. The efficacy of the seed treatments was highly variable across locations. Seed treatments (metalaxyl and mefenoxam) provided protection and increased yields across cultivars …


New National Extension Site Has Information On Dealing With Economic Crisis, Elbert Dickey, Dan Moser Dec 2008

New National Extension Site Has Information On Dealing With Economic Crisis, Elbert Dickey, Dan Moser

Institute of Agriculture and Natural Resources: News Releases

Lincoln, Ne & the nation's land-grant universities, including the University of Nebraska-Lincoln, have gathered myriad resources on-line to help Americans deal with the financial crisis.

The site is available through eXtension.org, via a link at www.extension.unl.edu (http://www.extension.unl.edu/).

Elbert Dickey, dean and director of UNL Extension, said the site is an excellent example of what eXtension does best.

"UNL Extension and our counterparts across the country created eXtension for just this purpose to get the 'best of the best' research-based, unbiased information from all over the U.S. into the hands of Americans who need it," Dickey said. "All Americans are struggling …


A High-Oleic-Acid And Low-Palmitic-Acid Soybean: Agronomic Performance And Evaluation As A Feedstock For Biodiesel, George L. Graef, Bradley J. Lavallee, Patrick Tenopir, Mustafa Tat, Bruce Schweiger, Anthony J. Kinney, Jon H. Van Gerpen, Thomas E. Clemente Dec 2008

A High-Oleic-Acid And Low-Palmitic-Acid Soybean: Agronomic Performance And Evaluation As A Feedstock For Biodiesel, George L. Graef, Bradley J. Lavallee, Patrick Tenopir, Mustafa Tat, Bruce Schweiger, Anthony J. Kinney, Jon H. Van Gerpen, Thomas E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Phenotypic characterization of soybean event 335-13, which possesses oil with an increased oleic acid content (> 85%) and reduced palmitic acid content (< 5%), was conducted across multiple environments during 2004 and 2005. Under these conditions, the stability of the novel fatty acid profile of the oil was not influenced by environment. Importantly, the novel soybean event 335-13 was not compromised in yield in both irrigated and non-irrigated production schemes. Moreover, seed characteristics, including total oil and protein, as well as amino acid profile, were not altered as a result of the large shift in the fatty acid profile. The novel oil trait was inherited in a simple Mendelian fashion. The event 335-13 was also evaluated as a feedstock for biodiesel. Extruded oil from event 335-13 produced a biodiesel with improved cold flow and enhanced oxidative stability, two critical fuel parameters that can limit the utility of this renewable transportation fuel.


Increased Survival Of Western Corn Rootworm On Transgenic Corn Within Three Generations Of On-Plant Greenhouse Selection, Lisa N. Meihls, Matthew L. Higdon, Blair D. Siegfried, Nicholas J. Miller, Thomas W. Sappington, Mark R. Ellersieck, Terrence A. Spencer, Bruce E. Hibbard Dec 2008

Increased Survival Of Western Corn Rootworm On Transgenic Corn Within Three Generations Of On-Plant Greenhouse Selection, Lisa N. Meihls, Matthew L. Higdon, Blair D. Siegfried, Nicholas J. Miller, Thomas W. Sappington, Mark R. Ellersieck, Terrence A. Spencer, Bruce E. Hibbard

Department of Entomology: Faculty Publications

To delay evolution of insect resistance to transgenic crops producing Bacillus thuringiensis (Bt) toxins, nearby ‘‘refuges’’ of host plants not producing Bt toxins are required in many regions. Such refuges are expected to be most effective in slowing resistance when the toxin concentration in Bt crops is high enough to kill all or nearly all insects heterozygous for resistance. However, Bt corn, Zea mays, introduced recently does not meet this ‘‘high-dose’’ criterion for control of western corn rootworm (WCR), Diabrotica virgifera virgifera. A greenhouse method of rearing WCR on transgenic corn expressing the Cry3Bb1 protein was used in …


Worm-Web Search: A Content-Based Image Retrieval (Cbir) System For The Parasite Image Collection In The Harold W. Manter Laboratory Of Parasitology, University Of Nebraska State Mueum, Ramalingamurthy Meduri, Ashok Samal, Scott Lyell Gardner Dec 2008

Worm-Web Search: A Content-Based Image Retrieval (Cbir) System For The Parasite Image Collection In The Harold W. Manter Laboratory Of Parasitology, University Of Nebraska State Mueum, Ramalingamurthy Meduri, Ashok Samal, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

We have developed a prototype web-accessible content-based image retrieval (CBIR) system that allows internet/web-based sharing of biological collections that contain large numbers of images of archived specimens. This system will enable both researchers and educators to access verified, high quality data on biological collections that are available in any museum with digitized holdings. The CBIR system that we are testing can play an important role in understanding global biodiversity because no knowledge of the specific names of specimens need be known before useful information can be extracted from such databases. Our CBIR framework allows users to search image collections using …


Cranes Of The World In 2008: A Supplement To Crane Music, Paul A. Johnsgard Dec 2008

Cranes Of The World In 2008: A Supplement To Crane Music, Paul A. Johnsgard

Papers in Ornithology

Time proceeds inexorably onward, and it has been 17 years since the first edition of Crane Music was published. During that time more than a billion people have been added to the earth's roles, and global warming has increasingly been recognized as a real tlu:oat to our planet's future. Although during that period a small percentage of Americans have become very rich through advances in technology, expanding markets and globalization, wildlife in general has suffered. Continuing population growth and associated economic and ecological pressures have resulted in greatly increased deforestation, wetland drainage, and destruction of natural habitats. Additionally, global climate …


Louis A. Fuertes And The Zoological Art Of The 1926–1927 Abyssinian Expedition Of The Field Museum Of Natural History, Paul A. Johnsgard Dec 2008

Louis A. Fuertes And The Zoological Art Of The 1926–1927 Abyssinian Expedition Of The Field Museum Of Natural History, Paul A. Johnsgard

Papers in Ornithology

The year 2009 marked the 110th anniversary of the first colored reproduction of a Fuertes painting; a watercolor of two seaside sparrows published in The Auk, when Fuertes was about 25 years old. Although Fuertes' life spanned little more than a half-century, and most living ornithologists were born after his tragic 1927 death, his influence on natural history art has not lessened. This manuscript is a testimony to his enduring artistic legacy.

I first looked in awe at the original set of Fuertes paintings in the summer of 1995, during a visit to the Field Museum in conjunction with …


Effects Of Shielding Adenoviral Vectors With Polyethylene Glycol On Vector-Specific And Vaccine-Mediated Immune Responses, Eric A. Weaver, Michael A. Barry Dec 2008

Effects Of Shielding Adenoviral Vectors With Polyethylene Glycol On Vector-Specific And Vaccine-Mediated Immune Responses, Eric A. Weaver, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Many individuals have been previously exposed to human adenovirus serotype 5 (Ad5). This prior immunity has long been known to hinder its use for gene therapy and as a gene-based vaccine. Given these immunogenicity problems, we have tested whether polyethylene glycol (PEG) can blunt immune effects against Ad5 during systemic and mucosal vaccination. Ad5 vectors were covalently modified with 5-, 20-, and 35-kDa linear PEG polymers and evaluated for their ability to produce immune responses against transgene antigen products and the vector itself. We show that shielding Ad5 with different-sized PEGs generally reduces transduction and primary antibody responses by the …


Fall Field Report, August-November 2008, W. Ross Silcock Dec 2008

Fall Field Report, August-November 2008, W. Ross Silcock

Nebraska Bird Review

Of interest to many is evidence of northward movement of species which generally summer on the Great Plains. In addition to White-winged Dove and Scissor-tailed Flycatcher, both slowly establishing as breeders in Nebraska, significant numbers of Glossy Ibis continue to be found, probably associated with major increases in numbers of White-faced Ibis. Perhaps the biggest surprise this late summer and early fall was the large number of Lesser Goldfinches reported from the southern Panhandle.

Less exciting by far, but worthy of attention were reports of several Mute Swans in the Omaha area, including a duo of juveniles, and a couple …


Release Of Roadside Native Perennial Grasses Following Removal Of Yellow Starthistle, Stephen L. Young, Victor P. Claassen Dec 2008

Release Of Roadside Native Perennial Grasses Following Removal Of Yellow Starthistle, Stephen L. Young, Victor P. Claassen

West Central Research and Extension Center, North Platte

The ecological benefits of a roadside native perennial grass stand are compromised when invasive species become established. We evaluated the potential to regenerate existing native perennial grass stands populated with yellow starthistle (Centaurea solstitialis) on a roadside planting in the lowland hills of the interior Coast Range of northern California. The experiment was designed to determine the effects of mowing, burning, or herbicide spraying, alone and in combination, on the vegetative cover and density of native perennial grasses and C. solstitialis. The study site contained blue wildrye (Elymus glaucus) and purple needlegrass (Nassella pulchra) as well as C. solstitialis. Burn, …


Remembering Everett Gross And Lee Morris Dec 2008

Remembering Everett Gross And Lee Morris

Nebraska Bird Review

Everett Gross, NOU member since 1966, passed away on March 5, 2008, at the age of 88. Mildred, his wife of 65 years, preceded him in death by only a few months [see June 2007 NBR, p. 62]. Both were regular participants at the NOU spring and fall gatherings.

Lee Morris of Benedict was another loyal, longtime member of the NOU. Lee was 86 when he passed away on July 14, 2008. He is survived by his wife, Shirley, sons James, Steve (also an NOU member), and Tom, and daughter, Linda. Lee was an NOU officer in the 1960s, and …


Fall Field Days At Ashland Dec 2008

Fall Field Days At Ashland

Nebraska Bird Review

The Fall Field Days were held southeast of Ashland at the Carol Joy Holling Conference Center September 26-28. Field trips to Platte River and Schramm State Parks, Louisville and Memphis SRAs, Jack Sinn WMA, Fontenelle Forest, and Spring Creek Prairie were led by Kevin Poague, Clem Klaphake, Larry Einemann, Rick Schmid, and Don and Janis Paseka.

Hawk migration was in full swing, and we observed 10 species, including a number of Broad-wings and Swainson's. An American Golden-Plover was a standout among the dozen shorebird species, and 10 warbler species were found, including Black-throated Green and Canada. Total species count was …


Index To Volume 76 Dec 2008

Index To Volume 76

Nebraska Bird Review

Alberts, Byron 52

Aleman-Zometa, Jason 155

Alexander,

George 20

Irene 20

R. D. 79

Allen, Sue 20

Anderson, S. H. 80

Anhinga 113

Atwood, J. L. 80

Aubushon, Kathy 19

Audubon, John J. 84-85

Avocet, American 58, 87, 101, 142, 159


Subscription And Organization Information [December 2008] Dec 2008

Subscription And Organization Information [December 2008]

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal, and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $15 in the United States, $18 in Canada and $30 in all other countries, payable in advance. Single copies are $4 each, postpaid, in the United States, $5 in Canada, and $8 elsewhere. Send orders for back issues to Mary Lou Pritchard, NOU Librarian, c/o University of Nebraska State Museum, W-436 Nebraska Hall, Lincoln, NE 68588-0514.

Memberships in the NOU (on a calendar-year basis only): …


Nebraska Bird Review (December 2008) 76(4), Whole Issue Dec 2008

Nebraska Bird Review (December 2008) 76(4), Whole Issue

Nebraska Bird Review

Fall Field Report, August–November 2008 ... 134

The 2007 Nebraska Nest Report ... 155

Fall Field Days at Ashland ... 165

Remembering Everett Gross and Lee Morris ... 169

Book Review [Birding in the Northern Plains: The Ornithological Writings of Herbert Krause] ... 171

Index to Volume 76 ... 172

Subscription and Organization Information ... 183


The Human Immunodeficiency Virus Type 1 Envelope Confers Higher Rates Of Replicative Fitness To Perinatally Transmitted Viruses Than To Nontransmitted Viruses, Xiaohong Kong, John T. West, Hong Zhang, Danielle Shea, Tendai J. M'Soka, Charles Wood Dec 2008

The Human Immunodeficiency Virus Type 1 Envelope Confers Higher Rates Of Replicative Fitness To Perinatally Transmitted Viruses Than To Nontransmitted Viruses, Xiaohong Kong, John T. West, Hong Zhang, Danielle Shea, Tendai J. M'Soka, Charles Wood

Nebraska Center for Virology: Faculty Publications

Selection of a minor viral genotype during perinatal transmission of human Immunodeficiency virus type 1 (HIV-1) has been observed, but there is a lack of information on the correlation of the restrictive transmission with biological properties of the virus, such as replicative fitness. Recombinant viruses expressing the enhanced green fluorescent protein or the Discosoma sp. red fluorescent (DsRed2) protein carrying the V1 to V5 regions of env from seven mother-infant pairs (MIPs) infected by subtype C HIV-1 were constructed, and competition assays were carried out to compare the fitness between the transmitted and nontransmitted viruses. Flow cytometry was used to …


Presentation Of The 2008 Asp Distinguished Service Award To William C. Campbell, Robin M. Overstreet Dec 2008

Presentation Of The 2008 Asp Distinguished Service Award To William C. Campbell, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Presentation of the 2008 American Society of Parasitologists Distinguished Service Award to William C. Campbell, Dana Fellow for scientists emeriti at Drew University.


Chlorovirus-Mediated Membrane Depolarization Of Chlorella Alters Secondary Active Transport Of Solutes, Irina V. Agarkova, David Dunigan, James R. Gurnon, Timo Greiner, Julia Barres, Gerhard Thiel, James L. Van Etten Dec 2008

Chlorovirus-Mediated Membrane Depolarization Of Chlorella Alters Secondary Active Transport Of Solutes, Irina V. Agarkova, David Dunigan, James R. Gurnon, Timo Greiner, Julia Barres, Gerhard Thiel, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the genus Chlorovirus. PBCV-1 infection results in rapid host membrane depolarization and potassium ion release. One interesting feature of certain chloroviruses is that they code for functional potassium ion-selective channel proteins (Kcv) that are considered responsible for the host membrane depolarization and, as a consequence, the efflux of potassium ions. This report examines the relationship between cellular depolarization and solute uptake. Annotation of the virus host Chlorella strain NC64A genome revealed 482 putative …


Ecological Niche Of The 2003 West Nile Virus Epidemic In The Northern Great Plains Of The United States, Michael Wimberly, Michael B. Hildreth, Stephen P. Boyte, Erik Lindquist, Lon Kightlinger Dec 2008

Ecological Niche Of The 2003 West Nile Virus Epidemic In The Northern Great Plains Of The United States, Michael Wimberly, Michael B. Hildreth, Stephen P. Boyte, Erik Lindquist, Lon Kightlinger

Public Health Resources

Background: The incidence of West Nile virus (WNv) has remained high in the northern Great Plains compared to the rest of the United States. However, the reasons for the sustained high risk of WNv transmission in this region have not been determined. To assess the environmental drivers of WNv in the northern Great Plains, we analyzed the county-level spatial pattern of human cases during the 2003 epidemic across a seven-state region.
Methodology/Principal Findings: County-level data on WNv cases were examined using spatial cluster analysis, and were used to fit statistical models with weather, climate, and land use variables as predictors. …


Acceptance Of The Clark P. Read Mentor Award: Students, Opportunity, Serendipity, And W.B. Yeats: "Education Is Not The Filling Of A Pail; It Is The Lighting Of A Fire", Donald W. Duszynski Dec 2008

Acceptance Of The Clark P. Read Mentor Award: Students, Opportunity, Serendipity, And W.B. Yeats: "Education Is Not The Filling Of A Pail; It Is The Lighting Of A Fire", Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Transcript of the speech given by Donald W. Duszynski, of the University of New Mexico, upon acceptance of the American Society of Parasitologists' Clark P. Read Mentor Award, 2008.


Shaping The Cross Timbers With Fire And Grazing, John A. Guretzky Dec 2008

Shaping The Cross Timbers With Fire And Grazing, John A. Guretzky

Department of Agronomy and Horticulture: Faculty Publications

Recently, our agricultural research team has been asked to provide talks during tours of our Oswalt Road Ranch. The Oswalt Road Ranch is a 4,992-acre property in Love County, Okla., willed to us by D. Joyce Coffey. The Noble Foundation took full control of the property in 2000. The ranch is a valuable piece of property because of its unique natural beauty and potential to support livestock production and wildlife habitat. On the tours, we have been showing off our new state-of-the art livestock handling facilities and providing information about results from recent research on by-product feeds, the Noble Foundation …


Ard News December 2008 Dec 2008

Ard News December 2008

Agricultural Research Division: News and Annual Reports

Contents:

Comments from the Dean Gary Cunningham
Undergraduate Honors Research Program
David H. and Annie E. Larrick/William G. Whitmore Funds, 2009
Mussehl Endowment
New or Revised Projects September and October 2008
Proposals Submitted for Federal Grants September and October 2008
Grants and Contracts Received for September and October 2008


The 2007 Nebraska Nest Report, Wayne J. Mollhoff Dec 2008

The 2007 Nebraska Nest Report, Wayne J. Mollhoff

Nebraska Bird Review

Rains returned to most of the state this breeding season, with the notable exception of almost the entire Panhandle and the western Sandhills, where the 8-year drought continues. In the western Sandhills some early rains began to restore water levels in the potholes and lakes, but levels fell again as the summer wore on. While recovering water levels in much of the Rainwater Basin brought renewed breeding activity by at least some of the marsh and water birds, continued dry conditions in the western Sandhills curtailed breeding by many water bird species there, with reduced or no breeding by grebes …


Book Review [Birding In The Northern Plains: The Ornithological Writings Of Herbert Krause], Paul A. Johnsgard Dec 2008

Book Review [Birding In The Northern Plains: The Ornithological Writings Of Herbert Krause], Paul A. Johnsgard

Nebraska Bird Review

Birding in the Northern Plains: The Ornithological Writings of Herbert Krause. Ronald R. Nelson, editor. 2008. The Center for Western Studies, Augustana College, Sioux Falls, SD. 252 pp. ISBN 978-0-9312170-87-4. Price: $27.50.

Herbert Krause was a native of Minnesota who spent most of his life in South Dakota, teaching at Augustana College from 1938 until his death in 1976. During that time he wrote extensively on South Dakota birds, much of which appeared in South Dakota Bird Notes, the journal of the South Dakota Ornithologists' Union. This book, assembled and edited by his long-time friend Ronald Nelson, brings …


A New Species Of The Genus Dendroleon Brauer From Mexico (Neuroptera: Myrmeleontidae), Lionel A. Stange Dec 2008

A New Species Of The Genus Dendroleon Brauer From Mexico (Neuroptera: Myrmeleontidae), Lionel A. Stange

Insecta Mundi

Abstract. A new species of Dendroleon Brauer is described from Mexico and is included in a key to three species of North America. Diagnoses and distributional data are given for D. obsoletus (Say) and D. speciosus Banks.

Resumen. Una nueva especie de Dendroleon Brauer es descripta de México y está incluída en una clave para las tres especies de Dendroleon de Norte America. Se provee una diagnosis para D. obsoletus (Say) y D. speciosus Banks además de datos sobre distribución geográfica.


Agrilinellus, New Genus And Four New Species Of Mexican Aphodiini (Scarabaeoidea: Scarabaeidae: Aphodiinae), Marco Dellacasa, Giovanni Dellacasa, Robert D. Gordon Dec 2008

Agrilinellus, New Genus And Four New Species Of Mexican Aphodiini (Scarabaeoidea: Scarabaeidae: Aphodiinae), Marco Dellacasa, Giovanni Dellacasa, Robert D. Gordon

Insecta Mundi

Agrilinellus, new genus is created for Agrilinus azteca (Harold) (type species), A. chiapasensis (Galante, Stebnicka and Verdú), and A. ornatus (Schmidt). Four new species from Mexico are described: Agrilinellus abbonai, Agrilinellus antonioreyi, Agrilinellus nuriae, Agrilinellus oaxacaensis.


College Of Agricultural Sciences And Natural Resources, 15th Annual Report August 1, 2007-July 31, 2008 Dec 2008

College Of Agricultural Sciences And Natural Resources, 15th Annual Report August 1, 2007-July 31, 2008

College of Agricultural Sciences and Natural Resources (CASNR): Annual Reports

Contents
Introduction 3 • Dedication 5 • Administration and Staff 6 • Academic Unit Administrators 6 • CASNR Standing Committees 7 • Faculty Advisory Council 7 • Student Advising Improvement Committee 8 • Teaching and Learning Improvement Council 9 • Scholarship and Graduation with Distinction Committee 9 • Curriculum Committee 10 • Teaching Faculty 11 • Faculty Meetings 11 • New Faculty or Faculty with New Appointments 11 • Retirements 12 • Faculty Awards 12 • Departmental News and Events 14 • Agricultural Economics 15 • Agricultural Leadership, Education and Communication 18 • Agronomy and Horticulture 20 • Animal Science …


Extended Visions, November/December 2008 Dec 2008

Extended Visions, November/December 2008

Extended Visions Newsletter of ARDC

Contents:
ARDC FEATURE UNIT - Feedmill
About the People
Mill Provides Feedstuffs for Research Projects
Ag Learning at the Festival
Eastern Nebraska Goat Workshop
Look to the Sky.... Observatory Offers Public Viewing Night - Nov. 7
Nebraska Cattlemen Visit the ARDC
Mead Magnet School
Click on the red barn and help out FFA!
UNL Representative helps Mead Students Discover Career Possibilities
Nebraska Soybean Day and Machinery Expo
Reverse Trade Mission
Saunders County Exhibitors Win Top Honors at Ak-sar-ben
The Clover Corner - What’s Going On With 4-H...
2008 Summer Field Days Results....
Utilizing Cornstalks and Other Crop Residue to Manage …


New Genes Originated Via Multiple Recombinational Pathways In The Β-Globin Gene Family Of Rodents, Frederico Hoffmann, Juan Opazo, Jay F. Storz Dec 2008

New Genes Originated Via Multiple Recombinational Pathways In The Β-Globin Gene Family Of Rodents, Frederico Hoffmann, Juan Opazo, Jay F. Storz

Jay F. Storz Publications

Species differences in the size or membership composition of multigene families can be attributed to lineage-specific additions of new genes via duplication, losses of genes via deletion or inactivation, and the creation of chimeric genes via domain shuffling or gene fusion. In principle, it should be possible to infer the recombinational pathways responsible for each of these different types of genomic change by conducting detailed comparative analyses of genomic sequence data. Here, we report an attempt to unravel the complex evolutionary history of the β-globin gene family in a taxonomically diverse set of rodent species. The main objectives were: 1) …


Characterization Of Dna Sequences That Confer Complement Resistance In Leishmania Chagasi, Rebecca R. Dahlin-Laborde, Eric J. Scolaro, Nathan M. Romine, Amanda Ramer-Tait, Soi Meng Lei, Jeffrey K. Beetham Dec 2008

Characterization Of Dna Sequences That Confer Complement Resistance In Leishmania Chagasi, Rebecca R. Dahlin-Laborde, Eric J. Scolaro, Nathan M. Romine, Amanda Ramer-Tait, Soi Meng Lei, Jeffrey K. Beetham

Department of Food Science and Technology: Faculty Publications

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into highpassage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) …