Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
A Biosensor Assay For The Detection Of Mycobacterium Avium Subsp. Paratuberculosis In Fecal Samples, V. Kumanan, Sam R. Nugen, A.J. Baeumner, Y.F. Chang
A Biosensor Assay For The Detection Of Mycobacterium Avium Subsp. Paratuberculosis In Fecal Samples, V. Kumanan, Sam R. Nugen, A.J. Baeumner, Y.F. Chang
Sam R. Nugen
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (101 to 106) MAP cells and amplified using RT-PCR and identified by the LF biosensor and the microtiter plate assay. While the LF biosensor assay …