Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Use Of High Pressure Liquid Chromatography To Detect And Quantify Oxytetracycline Marks In Walleye Otoliths And Dorsal Spines, Michael J. Hawkins
Use Of High Pressure Liquid Chromatography To Detect And Quantify Oxytetracycline Marks In Walleye Otoliths And Dorsal Spines, Michael J. Hawkins
Electronic Theses and Dissertations
Oxytetracycline (OTC) is used to chemically mark calcified structures in walleye Stizostedion vitreum fry and fingerlings. Current visual methods for detection of these marks are subjective, and biologists are unable to quantify the amount of OTC in a sample. The goal of this study was to determine if high pressure liquid chromatography (HPLC) is a feasible and superior alternative for detecting and quantifying OTC in otoliths of fry-marked and spines of fingerlingmarked walleyes. Study objectives were to validate methodology of extraction of OTC from calcified tissue, detect and quantify extracted OTC using HPLC, and determine a cost comparison of current …