Life Sciences Commons^™

A Comparative Gene Map Of The Horse (Equus Caballus), Alexandre R. Caetano, Yow-Ling Shiue, Leslie A. Lyons, Stephen J. O'Brien, Thomas F. Laughlin, Ann T. Bowling, James D. Murray

Biology Faculty Articles

A comparative gene map of the horse genome composed of 127 loci was assembled based on the new assignment of 68 equine type I loci and on data published previously. PCR primers based on consensus gene sequences conserved across mammalian species were used to amplify markers for assigning 68 equine type I loci to 27 horse synteny groups established previously with a horse-mouse somatic cell hybrid panel (SCHP, UC Davis). This increased the number of coding genes mapped to the horse genome by over 2-fold and allowed refinements of the comparative mapping data available for this species. In conjunction with …

Extensive Conservation Of Sex Chromosome Organization Between Cat And Human Revealed By Parallel Radiation Hybrid Mapping, William J. Murphy, Shan Sun, Zhang-Qun Chen, Jill Pecon-Slattery, Stephen J. O'Brien

Biology Faculty Articles

A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. Within the blocks, the feline, human, and mouse marker order was strongly conserved. Similarly, Y chromosome locus order was remarkably conserved between cat and human …

A Near-Surface Microstructure Sensor System Used During Toga Coare. Part Ii: Turbulence Measurements, Alexander Soloviev, Roger Lukas, Peter Hacker, H. Schoeberlein, M. Baker, A. Arjannikov

Marine & Environmental Sciences Faculty Articles

New techniques developed for near-surface turbulence measurements during the Tropical Ocean Global Atmosphere (TOGA) Coupled Ocean–Atmosphere Response Experiment (COARE) employ a difference in spatial scales of turbulence and surface waves. According to this approach, high relative speed of the measurements provides separation of the turbulence and surface wave signals. During the TOGA COARE field studies, highresolution probes of pressure, temperature, conductivity, fluctuation velocity, and acceleration were mounted on the bow of the vessel at a 1.7-m depth in an undisturbed region ahead of the moving vessel. The localization in narrow frequency bands of the vibrations of the bow sensors allows …

The Coral Reef Fishes Of Broward County Florida, Species And Abundance: A Work In Progress, B. D. Ettinger, David S. Gilliam, L. K. B. Jordan, Robin L. Sherman, Richard E. Spieler

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

The inshore environment of Broward County, FL consists of three coral reef/hard bottom reef tracts, separated by sand substrate, running parallel to the coast in sequentially deeper water. At quarter nautical mile intervals, for a five mile coastline section, fishes were censused at western, eastern and crest sites of each of the three reef tracts. On SCUBA and using the Bohnsack/Bannerot point-count method, we recorded: fish abundance, species richness, size, and general habitat of an imaginary cylinder 15 m in diameter. The position of each site was recorded by DGPS after census. One hundred and eighty-one sites were censused during …

North Fork Of The New River Benthic Macroinvertebrate Recruitment Study: Final Report, Charles G. Messing

Marine & Environmental Sciences Faculty Reports

Over the last several years, the Broward County Department of Natural Resource Protection has identified a series of major environmental problems in the North Fork of the New River, including illegal wastewater sludge discharge and heavy metal contamination of sediments (DNRP 1993 1994). A qualitative survey suggests that benthic macroinvertebrates have been negatively affected (DNRP 1997). Restoration projects have included removal in the summer of 1997 of a remnant sludge blanket from an area of the channel east of Interstate 95 and north of Broward Boulevard. However, the effectiveness of such dredging in terms of habitat restoration has not previously …

Isotope Ratio Studies Of Marine Mammals In Prince William Sound, Donald M. Schell, Amy Hirons

Marine & Environmental Sciences Faculty Reports

No abstract provided.

Fall 1999, Nsu Oceanographic Center

Currents

No abstract provided.

Final Technical Report For The Enhancement Of Autonomous Marine Vehicle Testing In The South Florida Testing Facility Range, South Florida Ocean Measurement Center (Sfomc) Partners

Marine & Environmental Sciences Faculty Reports

The purpose of this grant was to carry out the six scientific experiments on the South Florida Testing Facility (SFTF) Range. In addition to the enhancements to the range, work was performed on all six with some being successfully completed while research continues on the long term tasks.

Phenotypic Expressions Of Ccr5-Δ32/Δ32 Homozygosity, Giang T. Nguyen, Mary Carrington, Judith A. Beeler, Michael Dean, Louis M. Aledort, Philip M. Blatt, Alan R. Cohen, Donna Dimichele, M. Elaine Eyster, Craig M. Kessler, Barbara Konkle, Cindy Leissinger, Naomi Luban, Stephen J. O'Brien, James J. Goedert, Thomas R. O'Brien

Biology Faculty Articles

Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1.

Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis.

Results: …

Moolapheonoides Utmas, New Species, From Coral Reefs In The Madang Lagoon, Papua New Guinea (Amphipoda, Cyproideidae), James Darwin Thomas

Marine & Environmental Sciences Faculty Articles

Moolapheonoides utmas, new species, is described from coral reefs at Madang, Papua New Guinea. M. utmas differs from other species in the genus by the large tooth-cusp projecting posteroventrally on article 2 of pereopod 7 and the lower dorsal crest of the urosome. It shares an unusual dorsolateral bulbous projection of pereonite segments 3 and 4 with other cyproideid species Hoplopohoenoides obessa Shoemaker from Florida, and Naraphoenoides mullaya Barnard from Australia. Identification keys for the genus Moolaphoenoides are presented and relationships to other genera in the family are discussed.

Summer 1999, Nsu Oceanographic Center

Currents

No abstract provided.

Effects Of Plasma Hiv Rna, Cd4+ T Lymphocytes, And The Chemokine Receptors Ccr5 And Ccr2b On Hiv Disease Progression In Hemophiliacs, Eric S. Daar, Sharyne Donfield, Edward Gomperts, Margaret Hilgartner, Keith Hoots, David Chernoff, Cheryl Winkler, Stephen J. O'Brien, Hemophilia Growth And Development Study

Biology Faculty Articles

We have investigated the effects of plasma HIV RNA, CD4⁺ T lymphocytes and chemokine receptors CCR5 and CCR2b on HIV disease progression in hemophiliacs. We prospectively observed during follow-up 207 HIV-infected hemophiliacs in the Hemophilia Growth and Development Study. Plasma HIV RNA was measured on cryopreserved plasma from enrollment using the Chiron Corporation bDNA (version 2.0) assay. Genotype variants CCR2b-641 and CCR5-Δ32 were detected using standard molecular techniques. Those with the mutant allele for CCR2b, and to a lesser extent CCR5, had lower plasma HIV RNA, and higher CD4⁺ T lymphocytes than did those without these …

Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi

Marine & Environmental Sciences Faculty Articles

Sponges are well known to harbor large numbers of heterotrophic microbes within their mesohyl. Studies to determine the diversity of these associated microbes have been attempted for only a few shallow water species. We cultured various microorganisms from several species of Discodermia collected from deep water using the 'Johnson-Sea-Link' manned submersibles, and characterised them by standard microbiological identification methods. Characterisation of a small proportion (ca. 10%) of the total and potential eubacterial isolate collection with molecular systematics techniques revealed a wide diversity of microbes. Phylogenetic analyses of 32 small subunit (SSU) 16S-like rRNA gene sequences from different micorbes indicated high …

Spring 1999, Nsu Oceanographic Center

Currents

No abstract provided.

Epi-And Mesopelagic Fishes, Acoustic Data, And Sst Images Collected Off Lanzarote, Fuerteventura, And Gran Canaria, Canary Islands, During Cruise "La Bocaina 04-97", F. Bordes, F. Uiblein, R. Castillo, A. Barrera, J. J. Castro, J. Coca, J. Gomez, K. Hansen, V. Hernandez, N. Merrett, M. Miya, T. Moreno, F. Perez, A. Ramos, Tracey Sutton, M. Yamaguchi

Marine & Environmental Sciences Faculty Reports

During cruise "La Bocaina 0497" a series of 14 tows with a commercial pelagic trawl at depths between 20 and 700 m and an acoustic survey with a SIMRAD EK-500 echosounder were carried out in neritic and adjacent oceanic waters off Lanzarote, Fuerteventura, and Gran Canaria, Canary islands. In addition, SST images were obtained to get some informations on the prevailing hydrological conditions in the study area.

Of the 14 trawl tows ten were successful and resulted in capture of a total of 2166 fishes belonging to 81 species, 53 genera and 28 families. Ten species proved to be new …

Sea Turtle Conservation Program, Broward County, Fl 1999 Report, Curtis M. Burney, William E. Margolis

Marine & Environmental Sciences Faculty Reports

No abstract provided.

Skeletal Architecture And Density Banding Analysis Technique For Diploria Strigosa By X-Ray Computed Tomography, Kevin P. Helmle, Richard E. Dodge (Editor)

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Coral skeletal density information is a useful growth parameter and may be coupled with extension rates to determine calcification rates. The conventional process for density determination is often cumbersome and requires some parameters which are difficult to precisely specify (e.g., Mass absorption coefficients). We have developed a technique and windows based computer program which enables the rapid collection of coral density data. The coral skeleton slab is xradiographed with an aluminum wedge. Thickness and density of the aluminum wedge, thickness of the coral slab, density of pure coral aragonite, and digitized images of the coral and wedge x-radiographs provide necessary …

Winter 1999, Nsu Oceanographic Center

Currents

No abstract provided.