Life Sciences Commons^™

Degradation Of Hypomodified TrnaIMet In Vivo Involves Rna-Dependent Atpase Activity Of The Dexh Helicase Mtr4p, Xuying Wang, Huijue Jia, Eckhard Jankowsky, James T. Anderson

Biological Sciences Faculty Research and Publications

Effective turnover of many incorrectly processed RNAs in yeast, including hypomodified tRNA_i ^Met, requires the TRAMP complex, which appends a short poly(A) tail to RNA designated for decay. The poly(A) tail stimulates degradation by the exosome. The TRAMP complex contains the poly(A) polymerase Trf4p, the RNA-binding protein Air2p, and the DExH RNA helicase Mtr4p. The role of Mtr4p in RNA degradation processes involving the TRAMP complex has been unclear. Here we show through a genetic analysis that MTR4 is required for degradation but not for polyadenylation of hypomodified tRNA_i ^Met. A suppressor of the trm6-504 mutation …