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- Sitophila (1)
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Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
Predicting The Identities Of Su(Met-2) And Met-3 In Neurospora Crassa By Genome Resequencing, Kevin Mccluskey, Daren Brown, Erin Bredeweg, Scott E. Baker
Predicting The Identities Of Su(Met-2) And Met-3 In Neurospora Crassa By Genome Resequencing, Kevin Mccluskey, Daren Brown, Erin Bredeweg, Scott E. Baker
Fungal Genetics Reports
A significant number of classical genetic Neurospora crassa biochemical mutants remain anonymous, unassociated with a physical genome locus. By utilizing short read next-generation sequencing methods, it is possible to sequence the genomes of mutant strains rapidly and economically for the purpose of identifying genes associated with mutant phenotypes. We have taken this approach to connect genes and mutations to “methionineless” phenotypes in N. crassa.
A Protocol For Genetic Analysis At Different Stages Of The Nuclear Division Cycle In Neurospora Crassa, Kotaro Tsukada, Shin Hatakeyama, Shuuitsu Tanaka
A Protocol For Genetic Analysis At Different Stages Of The Nuclear Division Cycle In Neurospora Crassa, Kotaro Tsukada, Shin Hatakeyama, Shuuitsu Tanaka
Fungal Genetics Reports
The filamentous fungus Neurospora crassa is an organism that contains multiple nuclei in the asexual conidia and hyphae. Since the nuclei of dormant conidia are arrested at various points in the nuclear division cycle, it has been difficult to analyze drug sensitivity at the specific point of the cycle in N. crassa. In this study, we have established a useful method for analysis at different stages of the nuclear division cycle in N. crassa. This assay will be a reference for researchers to use the synchronized culture in other diverse analyses.
Neurospora 2023 - Program And Abstracts, Neurospora Conference
Neurospora 2023 - Program And Abstracts, Neurospora Conference
Fungal Genetics Reports
Neurospora 2023 - Program and Abstracts
Transposon- And Genome Dynamics In The Fungal Genus Neurospora: Insights From Nearly Gapless Genome Assemblies, Diem Nguyen, Valentina Peona, Per Unneberg, Alexander Suh, Patric Jern, Hanna Johannesson
Transposon- And Genome Dynamics In The Fungal Genus Neurospora: Insights From Nearly Gapless Genome Assemblies, Diem Nguyen, Valentina Peona, Per Unneberg, Alexander Suh, Patric Jern, Hanna Johannesson
Fungal Genetics Reports
A large portion of nuclear DNA is composed of transposable element (TE) sequences, whose transposition is controlled by diverse host defense strategies in order to maintain genomic integrity. One such strategy is the fungal-specific Repeat-Induced Point mutation (RIP) that hyper-mutates repetitive DNA sequences. While RIP is found across Fungi, it has been shown to vary in efficiency. The filamentous ascomycete Neurospora crassa has been a pioneer in the study of RIP, but data on TEs and RIP from other species in the genus is limited. In this study, we investigated 18 nearly gapless genome assemblies of ten Neurospora species, which …
On Computing Relative Effective Population Size Estimates And Parameters From An Equilibrium Cycle Of Hermaphrodite Frequency Fluctuation Due To Mixed Reproductive Modes In Filamentous Fungi, Christopher Toomajian
On Computing Relative Effective Population Size Estimates And Parameters From An Equilibrium Cycle Of Hermaphrodite Frequency Fluctuation Due To Mixed Reproductive Modes In Filamentous Fungi, Christopher Toomajian
Fungal Genetics Reports
Many filamentous ascomycete fungi reproduce primarily asexually, with only occasional sexual generations. This can lead to a departure from the 1:1 mating type ratio that is expected in obligate sexual populations. The relaxed selection on sexual traits also can lead to a decrease in the frequency of female fertile strains in field populations, while male fertility does not similarly decrease since male gametes also can serve as asexual spores. Both changes ultimately impact the strength of genetic drift in populations. The frequency of female sterility likely increases with the time since the last generation of sexual reproduction, such that it …
Relative Efficiency Of Split-Marker Versus Double-Crossover Replacement Protocols For Production Of Deletion Mutants In Strain Ph-1 Of Fusarium Graminearum, Sladana Bec, Gabdiel E. Yulfo-Soto, Lisa J. Vaillancourt
Relative Efficiency Of Split-Marker Versus Double-Crossover Replacement Protocols For Production Of Deletion Mutants In Strain Ph-1 Of Fusarium Graminearum, Sladana Bec, Gabdiel E. Yulfo-Soto, Lisa J. Vaillancourt
Fungal Genetics Reports
The split-marker (SM) protocol has become a popular method for production of knockout mutations in fungi. We used Southern hybridization to compare the performance and efficiency of the SM protocol with the more traditional double-crossover intact marker (IM) method for creating deletions of the mating type genes in Fusarium graminearum. Both methods successfully produced knockouts at a rate of between 24 and 75%: the SM method produced mutants more efficiently for larger constructs (>1 kb), but it was similar to IM for a smaller construct that was 865 bp. Both methods also produced strains with additional ectopic integrations …
Neurospora 2021 - Program And Abstracts, Neurospora Conference
Neurospora 2021 - Program And Abstracts, Neurospora Conference
Fungal Genetics Reports
Neurospora 2021 - Program and Abstracts
A Nonhomologous End-Joining Mutant For Neurospora Sitophila Research, Nicholas A. Rhoades, Elise K. Webber, Thomas M. Hammond
A Nonhomologous End-Joining Mutant For Neurospora Sitophila Research, Nicholas A. Rhoades, Elise K. Webber, Thomas M. Hammond
Fungal Genetics Reports
Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of Neurospora crassa and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: N. sitophila. Specifically, we show that deletion of N. sitophila mus-51 increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the N. sitophila mus-51∆ strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC, Kansas State University, Manhattan, KS).
Excellence By Simplicity: Life And Contributions Of Professor Ramesh Maheshwari (1940-2019), Keyur Adhvaryu
Excellence By Simplicity: Life And Contributions Of Professor Ramesh Maheshwari (1940-2019), Keyur Adhvaryu
Fungal Genetics Reports
Professor Ramesh Maheshwari passed in Bangalore on 30 March 2019. His work generated fundamental understanding in diverse areas of fungal biology including the germination and infection of plants by rust fungi, the life-cycle of Neurospora in its natural habitat; fungal senescence and heterokaryosis, and the production of microconidia in fungi. He was a member of many professional societies including the American Society of Plant Physiologists, Society of Microbiologists of India, and the Indian Phytopathological Society, and he served on the Editorial Board for Journal of Genetics. He nurtured scientific curiosity in his students and colleagues.